면역 억제 마우스 모델 및 Jurkat 세포에서 T세포 조절을 통한 괭생이모자반 추출물의 면역 활성 증가 효과

김동섭,정윤우,김민지,한인준,김건,김춘성,성낙윤 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.4

본 연구에서는 괭생이모자반 추출물의 면역증강 효과를 확인하기 위하여 CPA 유도를 통한 면역억제 동물모델과 T 세포주인 Jurkat을 활용하여 분석하였다. 동물모델에서 괭생이모자반 추출물 투여는 체중과 더불어 면역조절에 관여하는 중요한 조직인 비장 및 흉선에서 CPA에 의한 조직 무게 감소의 억제 효과가 있음을 확인하였다. 또한, 혈액학적 평가를 통해 백혈구, 림프구, 호중구, 단핵구의 수치와 면역글로불린인 IgM, IgA 및 IgG의 발현량이 괭생이모자반 추출물 투여를 통해 CPA에 의한 수치 감소를 억제하는 것을 확인하였다. 괭생이모자반 추출물을 투여는 CPA에 의한 비장 총 세포 수 및 NK 총 세포 수의 감소를 억제하였으며, 면역세포 활성 지표 인자인 IL-2 및 IFN-γ가 CPA군 대비 증가함을 확인하였다. 또한, NK 세포 활성화 분석을 통해 괭생이모자반 투여가 NK 세포 활성 증가를 유도할 수 있음을 확인하였다. 비장 조직에서 H&E 염색 및 면역조직화학 분석을 통해 조직학적 병변과 T 림프구의 대표 표현형인 CD3 및 CD4의 발현량을 측정한 결과, 괭생이모자반 추출물 투여는 WP 및 RP 영역이 CPA에 의해 감소하는 것을 억제하였으며, 면역조직화학 분석을 통해 CD3 및 CD4의 발현량이 CPA군 대비 증가하는 것을 확인하였다. T세포(Jurkat) 모델에서 괭생이모자반 추출물 처리는 T세포 증식능을 증가시키고, 과민면역반응의 지표인 IL-4 분비능의 증가 없이 IL-2/IFN-γ 사이토카인 분비능을 증가시키는 것으로 확인되었으며, 이는 세포 증식 및 cytokines 분비와 밀접한 관련을 하는 MAPK의 세포 내 조절을 통하여 이루어짐을 확인하였다. 이러한 결과로 미루어 보아 괭생이모자반 추출물의 투여는 T세포 및 B세포의 활성을 통하여 CPA 유도 면역저하 동물모델에서 면역증강 효능을 나타내는 것으로 사료된다. 괭생이모자반 추출물은 면역 활성 유도 기능성과 관련하여, 건강기능식품 원료로 개발하는 데 있어 그 잠재적 가치가 매우 클 것으로 사료된다. Recent epidemics of immune diseases have increased interest in foods that can regulate immunity. This study examined the effects of Sargassum horneri extract (SH) on the immune function in in vivo and in vitro models. The SH treatment inhibited the decrease in body, spleen, and thymus weights in cyclophosphamide (CPA) induced immune-suppressed animal models. It improved the total number of white blood cells, neutrophils, lymphocytes, and monocytes, as well as the levels of immunoglobulin (Ig) M, IgG, and IgA, which are indicators of immune-enhancing activity in the blood. The natural killer (NK) cell activity is an essential indicator of immune activity, and SH administration increased the number of spleen cells and NK cells in CPA-treated immune-suppressed mice. In addition, Jurkat cells were used, as a T cell line to verify the immune activation mechanism of SH. The results showed that the SH treatment increased cell proliferation, cytokine production (IL-2 and INF-γ), and protein expression of MAPK. Overall, SH has value as a material for functional foods because of its immune-enhancing efficacy.

자기전해연마공정의 복합파라미터 최적화에 관한 연구

김동섭,최민석,김정두 한국생산제조학회 1995 한국생산제조시스템학회 학술발표대회 논문집 Vol.1995 No.6

축류터빈의 관통유동해석 - 다유선해석과 평균반경해석의 비교분석 -

김동섭,Kim, Tong Seop 대한기계학회 1998 大韓機械學會論文集B Vol.22 No.8

A throughflow analysis program for axial flow turbines is constructed, which can handle not only the two-dimensional multi-streamline (streamline curvature) method but also the one-dimensional mean line method. Calculations are performed for single stage and multi-stage axial flowturbines. For a wide operating range, the performance and flow field calculated by the present streamline curvature method are close enough to the test data. It is also revealed for the single stage turbine that the present analysis leads to far better correspondence with the experiment than other researchers" throughflow analyses. A special focus is put on the comparison of the results between the streamline curvature analysis and the mean line analysis. It is found that the mean line analysis can not predict the performance for highly off-designed conditions as accurately as the streamline curvature method, which shows the importance of considering the spanwise variation of loss and flow.

Antimicrobial Activity of Various Parts of Tomato Plants Varied with Different Solvent Extracts

김동섭,Yurina Kwack,Jung Heon Lee,Changhoo Chun 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.2

The antimicrobial activity of acetone, hexane, dichloromethane, and methanol extracts from leaves, stems, immature green fruits, and red fruits of tomato plants was examined against six phytopathogens. The minimum inhibitory concentration (MIC) of the acetonic extracts from these four plant parts was lower than that of the other solvents. Among the acetonic extracts, tomato leaves had a lower MIC than the other tomato parts. The acetonic extract from tomato leaves was therefore selected as a source of antimicrobial substances. The acetonic extract from tomato leaves inhibited mycelial growth of Fusarium oxysporum f. sp. lycopersici, Glomerella cingulata, and Rhizoctonia solani. Mycelial growth of R. solani treated with acetone extract from leaves showed more susceptibility than the other phytopathogens. Using 0.31 mg/ml of the acetonic extract from leaves, mycelial growth of R. solani on days 1, 2, and 3 decreased by 50.0, 52.1, and 64.0%, respectively, compared with acetone solvent treatment. The antimicrobial compounds effective against R. solani were identified as linolenic acid and caffeic acid by bioautography and GC-MS. These two compounds were used to treat six phytopathogens to confirm their antimicrobial activities. Linolenic acid inhibited mycelial growth of R. solani, while caffeic acid showed only slight antimicrobial activity. Results indicated that we propose extracts from tomato leaves which included antimicrobial compounds may provide a new lead in the pursuit of new biological sources of agrochemical candidates.

비행청소년을 통해 본 언론매체와 청소년 범죄

김동섭 한국언론진흥재단 1992 新聞과 放送 Vol.- No.257

실내 환경에서 드론 군집 비행을 위한 모션 캡쳐 시스템의 마커 위치 인식률 개선

김동섭,김강민,이규만 제어로봇시스템학회 2024 제어로봇시스템학회 국내학술대회 논문집 Vol.2024 No.7

왕겨 유래 실리카 하이드로겔의 개질처리에 의한 침강실리카 특성 변화

김동섭,성용주 한국펄프.종이공학회 2022 펄프.종이기술 Vol.54 No.1

In this study, the changes in the properties of the prepared precipitated silica were evaluated depending on the conditions of the modification process of the silica hydrogel originated from rice husks. Rice husk silica hydrogel was prepared through a sol-gelation process after alkali soaking extraction of rice husk. The silica hydrogel was modified by adjusting the solution concentration and temperature conditions in acidic and alkali solutions. As a result, the changes in surface chemical properties and the improvement of quality characteristics such as whiteness and impurity removal were confirmed through the modification process. The modification treatment in an acidic atmosphere minimized the decrease in production yield and showed great effects on the whiteness and the removal of impurities such as alkali metals. In a alkaline condition, the improvement in the whiteness was clearly shown, but the reduction in yield increased. It was shown that the effects of the residual alkali metals and the organic substances on the properties of the precipitated silica could be controlled by the control of the modification process conditions. .

대학생의 개인별환경이 창업동기, 창업교육 및 창업의도에 미치는 영향-의정부 4년제, 2년제 대학을 중심으로-

김동섭 한국외식경영학회 2017 외식경영연구 Vol.20 No.6

The purpose of this study is to examine the effects of individual environmental factors of university students to entrepreneurial intentions, education, and motivations. Based on literature search, four variables which compose the decision of establishing business. They were individual environmental factor, entrepreneurial intentions, education, and motivations. These four variables were tested through statistical methodology such as reliability analysis, factor analysis, correlation analysis, and regression analysis. These results lead to the conclusion that is a Challenge Spirit, Riskiness, Initiative, Self realization, apply to aptitude, Family business succession, Fellow trader, Interdisciplinary Study Course, Participate in Club activities. The result of this study would help to explore and specify factors on entrepreneurial minds of university students. There is room for further investigation about policy, plan for support, Related bussiness in Entrepreneurial. 본 연구의 목적은 의정부시의 4년제, 2년제 대학생들을 대상으로 대학생의 개인별 환경이 창업동기, 창업교육 및 창업의도에 미치는 영향을 조사하였다. 본 조사는 설문조사를 통해 신뢰성 분석, 요인분석, 상관분석을 실시하였다. 본 조사는 문헌조사의 선행연구를 이용하여 연구의 가설을 정하고 4가지 요인으로 변수를 추출하였다. 4가지 요인은 개인별환경, 창업동기, 창업교육, 창업의도로 검증되었다. 결론적으로 4가지 요인을 통해 도출된 내용은 도전정신, 새로운일, 어려운 위험에 대한 모험정신, 커러큘럼, 자아실현, 적성적용, 가업승계, 동업친구, 연계전공교육, 동아리활동, 적극성 경력의 필요성 등이 창업동기,창업교육,창업의도에 영향을 주는 것으로 검증되었다. 이러한 연구조사를 기반으로 다른 사회적인 현상들을 구성하여 연구한다면 미래의 청년 창업의 솔루션으로 새로운 방안을 탐색할 수 있으리라 사료된다.

분산전원 연계변압기의 전압 탭을 고려한 Volt-Var 제어 곡선 적합성 연구

김동섭,김현진,조종민,백자현 대한전기학회 2022 전기학회논문지 Vol.71 No.10

This paper studies and proposes the Volt-Var control curve considering DER(Distributed Energy Resources) transformer voltage tap. As DER is increasing around the world, DER is required to have a system support function for power system stability. Recently, even in Korea, the power system support function is required for the DER. In particular, the Volt-Var reactive power function will be used to solve the overvoltage problem caused by the increase in generation of DER in distribution system. DER in the domestic distribution system uses a transformer to connect into the distribution system, and the transformer voltage tap is set differently depending on the system situation. Therefore, this paper analyzes the suitability of the Volt-Var control curve in the domestic system by considering the transformer voltage tap, and suggests a method for setting the Volt-Var control curve suitable for the domestic system

Relationship between spatial organization and biological function, analyzed using gene ontology and chromosome conformation capture of human and fission yeast genomes

김동섭,한승수 한국유전학회 2016 Genes & Genomics Vol.38 No.8

Cells regulate functionally related genes cisand trans-contacts in order to perform specific biological roles. To understand the cryptic spatial genomic contexts underlying these biological functions, we analyzed the gene association data from the gene ontology (GO) database and the genomic spatial organization data obtained by analysis of chromosome conformation capture (3C)-based data from the Sequence Read Archive, where GO and 3Cbased data were used to measure functional similarity and spatial proximity, respectively, between genomic loci. In the human genome and the fission yeast genome, we observed that correlation between the two measures was statistically significant on a genome-wide scale. Specifically, it is also confirmed that the genomic spatial architecture is affected by functional similarity of genes by showing better correlation of functional similarities with spatial distances estimated by contact frequencies than those estimated by genomic distances for cis-contacts. Furthermore, we analyzed distances between the genomic segments sharing the same GO term using the two-sample t test, found that the genomic segments identified by various GO terms are spatially located closer than the average distance over statistically-valid contacts, and provided a list of the GO terms. The results suggested that genomic loci with similar biological functions are situated in close proximity to each other in the nuclear space by aggregating functionally related genes in a short spatial range.