안와 연부조직에서 발생한 간엽성 연골육종-1증례 보고-

강유미,지미경,강석진,김병기,김선무 대한병리학회 1989 Journal of Pathology and Translational Medicine Vol.23 No.2

CHANGES IN BODY AND ORGAN WEIGHTS, HEMATOLOGICAL PARAMETERS, AND FREQUENCY OF MICRONUCLEI IN THE PERIPHERAL BLOOD ERYTHROCYTES OF ICR MICE EXPOSED TO LOW-DOSE-RATE -RADIATION

강유미,신석철,진영우,김희선 대한방사선방어학회 2009 방사선방어학회지 Vol.34 No.3

We exposed ICR mice to low-dose (0.2 Gy) and low-dose-rate (0.7 mGy/h) -radiation (137Cs) in the Low-dose-rate Irradiation Facility at the Radiation Health Research Institute to evaluate systemic effects of low-dose radiation. We compared the body and organ weights, number of blood cells (white and red blood cells and platelets), levels of biochemical markers in serum, and frequency of micronuclei in polychromatic erythrocytes between low-dose irradiated and nonirradiated control mice. The ICR mice irradiated with total doses of 0.2 and 2 Gy showed no changes in body and organ weights, number of blood cells (white and red blood cells), or frequency of micronuclei in the polychromatic erythrocytes of peripheral blood. However, the number of platelets (P = 0.002) and the liver weight (P < 0.01) were significantly increased in mice exposed to 0.2 and 2 Gy, respectively. These results suggest that a low-dose-rate of 0.7 mGy/h does not induce systemic damage. This dose promotes hematopoiesis in the bone marrow microenvironment and the proliferation of liver cells. In the future, the molecular biological effects of lower doses and dose rates need to be evaluated.

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.2

Survival Rate and Thymic Lymphoma Incidence in High- and Low-dose-rate Irradiated AKR/J Mice and Commonly Expressed Genes

강유미,신석철,김차순,양광희,진영우,김효윤,김희선 한국실험동물학회 2009 한국실험동물학회 학술발표대회 논문집 Vol.2009 No.2

Bioceramic-Poly D,L-Lactic-co-Glycolic Acid(PLGA) Scaffold에접종한 인간지방조직-유래중간엽줄기세포의골형성

강유미,홍순갑,도병록,김해권,이준영 한국발생생물학회 2011 발생과 생식 Vol.15 No.2

본실험은bioceramic을첨가하여만든다공성poly D,L-lactic-co-glycolic acid(PLGA)-scaffold가인간지방조직에 서유래된중간엽줄기세포(human adipose tissue derived mesenchymal stem cells, ATMSCs)의골형성과정에효과적인지를알아보고자수행하였다. ATMSCs를well plate에접종하여골형성유도(osteogenic induction, OI) 배양액으로28일동 안배양하였다. OI배양액군의증식률은세포접종후14일까지는세포증식이활발하게진행됐지만, 21일이후세포의 증식이둔화되는양상을보였다. 반면, 기본배양액군은 꾸준한세포증식을보이며21일이후에는OI배양액군 보다더높은증식을나타냈다. OI배양액군의 alkaline phosphatase(ALP) 활성은 세포배양 21일까지는 증가했지만, 8일에는 감소한 반면에 기본배양액군은 계속 감소하는 양상을 띠었다. OI배양액군의 세포는 배양 21일에는 뚜렷한 nodule의 형성을 관찰할 수 있었고, nodule에 칼슘의 축적이 일어남을 확인하였다. ATMSCs를 scaffold에 접종하여 OI배양액으로 배양하였다. Scaffold 내 골아세포 분화에 따른 ALP 활성은 PLGA scaffold와 Bioceramic-PLGA scaffold 모두에서 세포 배양 21일에 급격히 증가하였고, Bioceramic-PLGA scaffold의 ALP 활성이 PLGA scaffold보다 크게 증가하였다. 칼슘과 인의 함량 역시 Bioceramic-PLGA scaffold에서 높게 나타났으며, Bioceramic-PLGA scaffold의 Ca/P ratio가 PLGA scaffold보다 높게 나타났다. 생체내에 이식된 scaffold의 생분해성과 광물화는 bioceramic-PLGA scaffold에서 더욱 뚜렷하게 관찰되었다. 본 실험의 결과들을 종합해 볼 때 ATMSCs의 골형성능은 well plate보다 scaffold가 더 효과적이며, bioceramic이 scaffold의 세포 부착률과 ALP 활성을 증가시켜 골형성능에 효과적으로 작용하는 것으로 생각된다. 또한 bioceramic이 ATMSCs의 골형성 분화에 따른 광물화단계의 scaffold 내 칼슘과 인의 함량을 증가시키는 것으로 사료된다. 생체 내 scaffold의 생분해성은 PLGA scaffold보다 Bioceramic-PLGA scaffold가 빠른 분해를 나타내며, 광물화에 따른 칼슘 침착이 더 활발한 것은 scaffold에 포함된 bioceramic이 생체 내 세포의 부착, 증식, 분화를 증가시켜 골형성을 촉진시키는 물질로 작용한 것으로 사료된다.

고선량률 및 저선량률 전리방사선 조사된 AKR/J 마우스에서 특이하게 발현되는 암 진행도 관찰지표 유전자

강유미,신석철,양광희,김차순,김지영,남선영,진영우,최승진,김희선 대한방사선방어학회 2010 대한방사선방어학회 학술발표회 논문요약집 Vol.2010 No.11

Immune-related cytokine activations and suppression of ovarian cancer in low-dose irradiated C57BL6/N mice

강유미,최무현,최승진,김희선 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.8

Effects of Incretin-Based Therapies on Diabetic Microvascular Complications

강유미,정창희 대한내분비학회 2017 Endocrinology and metabolism Vol.32 No.3

The morbidity and mortality associated with diabetic complications impose a huge socioeconomic burden worldwide. Therefore, the ultimate goal of managing diabetes mellitus (DM) is to lower the risk of macrovascular complications and highly morbid microvascular complications such as diabetic nephropathy (DN) and diabetic retinopathy (DR). Potential benefits of incretin-based therapies such as glucagon-like peptide 1 receptor agonists (GLP-1 RAs) and dipeptidyl peptidase-4 (DPP-4) inhibitors on the diabetic macrovascular complications have been recently suggested, owing to their pleiotropic effects on multiple organ systems. However, studies primarily investigating the role of these therapies in diabetic microvascular complications are rare. Nevertheless, preclinical and limited clinical data suggest the potential protective effect of incretin-based agents against DN and DR via their anti-inflammatory, antioxidative, and antiapoptotic properties. Evidence also suggests that these incretin-dependent and independent beneficial effects are not necessarily associated with the glucose-lowering properties of GLP-1 RAs and DPP-4 inhibitors. Hence, in this review, we revisit the preclinical and clinical evidence of incretin-based therapy for DR and DN, the two most common, morbid complications in individuals with DM. In addition, the review discusses a few recent studies raising concerns of aggravating DR with the use of incretin-based therapies.

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.25 No.5

결혼이주여성의 성공적 적응과정에 관한 연구

강유미,신혜종 한국상담학회 2010 상담학연구 Vol.11 No.4

The purpose of this study was to provide an in-depth understanding of the successful adjusting process of the immigrant wives and their spouses. The authors have followed the procedure of the ‘Grounded Theory’ suggested by Strauss & Corbin (1998) with four couples who were introduced by the Multi Cultural Family Center staffs as couples ‘successfully settled’ into the community. In-depth interviews with semi-structured questionnaires were conducted and the collected data rendered 164 concepts and 27 sub-categories which was deduced to 11 categories. The followings are the key categories resulted by the paradigm axial coding. International marriage was suggested as the casual condition, ‘cultural difference’ and ‘conflict’ as the central phenomena. Contextural conditions that influenced the result were ‘advance information’ and ‘cultural similarity.’ Mediating conditions were ‘spouse support’, ‘family support’ and ‘community support.’ the Action/Interaction strategies were ‘multi-cultural understanding’ and ‘adjusting to each other.’ The end result ‘adaptation with stability’, and the key category ‘adjusting with understanding each others’ difference’ were also deduced from the data. The authors have induced the four stages to successful adjust of immigrant wives which follows: the stage of cultural differences and conflict; the stage of understanding and trying, the stage of adjusting to each other, and the stage of adjustment with stability. We also found two types of adjustment, ‘early adjusting group’ and ‘adjusting after some conflicts group.’ The result has shown that ‘cultural differences’ and ‘conflict’ was influenced by ‘advance information’ and ‘cultural similarity.’ The ‘multi-cultural understanding’ which is respecting each other’s culture, understanding and accepting each other as is was the key factor of the couple for the wives to adapt into the society. 본 연구는 국내에 거주하고 있는 국제결혼부부의 적응과정에서 결혼이주여성이 어떠한 경험을 하였는지를 탐색하고 성공적인 상호적응의 과정을 이해하기 위해서 수행되었다. 이를 위해 한국남성과 결혼하여 충남북부지역에 살고 있는 결혼이주여성과 그 배우자 4쌍을 대상으로 심층면접을 실시하고 근거이론방법을 적용하여 자료를 분석하였으며, 연구 결과는 다음과 같다. 결혼이주여성의 적응은 ‘갈등 후 적응’과 ‘초기 적응’의 두 가지 유형으로 나눌 수 있으며, 상호적응의 과정은 문화차이와 갈등 단계, 이해와 배려단계, 서로 맞추어 가는 단계, 안정된 적응단계의 4단계로 나타났다. 국제결혼부부의 성공적인 상호적응에서 중요한 핵심 범주는 ‘서로의 차이를 이해하고 맞추어 감’으로 나타났으며, ‘사전정보’ 와 ‘문화적 동질성’이 상호적응에 영향을 미치는 맥락적 조건으로 작용하였다. ‘배우자의 지지’와 ‘가족의 지지’, ‘사회적 지지’가 안정된 적응을 위한 중재적 조건으로 나타났다. 연구 결과를 토대로 후속 연구를 위한 제언을 하였다.