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이승은,정태영 한국농화학회 1991 Applied Biological Chemistry (Appl Biol Chem) Vol.34 No.4
The whole volatile flavor concentrate obtained from Jindalrae flower was separated into hydrocarbon and oxygen-containing compound(OCC) fractions, and the OCC-fraction was further separated by column chromatography into nine sub-fractions, respectively. These fractions were analyzed by gas chromatography and combined gas chromatography/mass spectroscopy. One hundred and sixty-two components, including 61 hydrocarbons, 18 aldehydes, 18 esters, 41 alcohols, 3 ketones, 4 oxides, 8 acids, 6 phenols and 3 miscellaneous components, were identified(Received October 28, 1991, accepted December 16, 1991).
이승은,최재훈,이정훈,노형준,김금숙,김진경,정해영,김승유 한국자원식물학회 2013 한국자원식물학회지 Vol.26 No.4
This study was conducted to select some useful plants as functional material candidates. A total of 38 plants were preliminarily screened for the anti-inflammatory and antioxidant activities. The preliminarily selected 8 plants were further investigated to verify the in vitro inhibitory effect on inflammation and oxidative stress. Boehmeria platanifolia (root),Carpinus coreana (branch), and Eupatorium japonicum (leaf) inhibited the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Eupatorium japonicum (leaf) suppressed the expression of cyclooxygenase-2 (COX-2), whereas Boehmeria platanifolia (root) and Prunus yedoensis (branch) inhibited the transcription of nuclear factor-kappa B (NF-κB). Treatment with the extracts (2.5 ~ 20 μg/ml) of Abutilon theophrasti (leaf,flower/seed) and Hemistepta lyrata (stem) did not show toxicity on RAW 264.7 cell proliferation, but treatment with 2.5 μg/ml of Boehmeria platanifolia (root) exhibited cell toxicity. Carpinus coreana (branch) and Prunus yedoensis (branch)showed potent scavenging activities on peroxynitrite. Akebia quinata (flower), Carpinus coreana (branch), and Prunus yedoensis (branch) effectively inhibited reactive oxygen species (ROS). Abutilon theophrasti (leaf), Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) exhibited strong inhibitory capacity with regard to nitric oxide (NO) production. The results suggested that Abutilon theophrasti (leaf) has in vitro anti-inflammatory and antioxidant activities, and that is a useful functional material candidate. 본 연구는 식물자원을 재료로 하여 in vitro 항염증 및 항산화 활성을 분석함으로써 기능성소재로의 활용 가능성이있는 유망 후보자원을 발굴하고자 수행하였다. 이를 위해식물추출물 38종을 대상으로 세포증식에 대한 영향, 염증관련지표(nitric oxide, TNF-α, IL-6, IκBα, iNOS, COX-2)및 항산화항목(DPPH 라디칼 소거능, LDL 산화저해능) 및총페놀 함량에 대한 효과를 탐색하고 결과로부터 8종의 시료를 1차로 선발하였다. 선발된 8종의 시료에 대해서는 다시 농도별로 세포증식에 대한 효과, iNOS, COX-2의 발현및 NF-κB 전사에 대한 영향, peroxynitrite, ROS, nitricoxide 생성에 대한 IC50 값을 분석하여 보다 가능성이 있는식물을 유망후보자원으로 선발하고자 하였다. 그 결과, 소사나무(가지), 등골나물(잎), 으름(꽃) 및 개모시풀(뿌리)은항염증 혹은 항산화활성이 우수하나 농도에 따라 세포독성을 나타내었으므로 활용에 주의가 필요해보였으며, 어저귀(잎)은 항염증활성과 항산화활성이 비교적 우수하고 실험된 처리농도에서 세포독성이 없어 향후 in vivo 활성 검정등 심화연구를 통해 그 활용 가능성을 검토할 필요가 있다고 사료되었다.
온라인 활동 참여와 미디어 이용행태가 온라인 프라이버시 침해 우려에 끼치는 영향 연구
이승은 인문사회 21 2019 인문사회 21 Vol.10 No.3
In modern society, the advancement of technology and the Internet have become important causes of online privacy violation cases. This study analyzes the effects of online media participation and media utilization ability on online privacy violation using 2017 Korean Media Panel survey data, and also analyzes gender differences. Results show that individuals with more online cafe (forum) activities, a good command of basic PC abilities and smart devices, and lower PC utilization are likely to attract a higher possibility of privacy violation. In addition, those with higher PC abilities and smart devices for basic functions and lower PC abilities on an expert level are more likely to feel anxious about online privacy infringement, which was observed in both full and gendered models. The result of considering gender differences shows that individuals with good basic PC abilities and smart devices and lower abilities of higher levels of such devices living in a non-urban area are prone to attack risks of privacy infringement. The research also provided implications based on the study results. 현대사회에서는 첨단기술 및 사물인터넷의 발전으로 인해 온라인 프라이버시 침해 사례가 발생하고 있다. 본 연구는 2017년 한국미디어패널조사 자료를 활용하여 온라인 활동 참여 및 미디어 활용능력의 온라인 프라이버시 침해에 대한 영향을 분석하며, 성별 차이 분석도 진행하였다. 분석결과, 카페 활동에 적극적이고, 우수한 PC・스마트기기의 기본능력과 낮은 PC 고난도 활용수준일수록, 프라이버시 침해 우려 정도가 높을 가능성이 큰 것으로 나타났다. 또한, PC・스마트기기의 기본능력이 우수하고, PC 고난도 활용수준이 낮을수록 온라인 프라이버시 침해 우려가 컸으며, 이는 성별 차이를 고려하지 않은 분석모델 및 남녀집단 모두에서 공통적으로 나타났다. 성별 차이를 고려한 결과는, PC・스마트기기의 기본능력이 우수하나, PC 및 스마트기기의 고난도 활용수준이 낮을수록, 비도시거주자일수록 프라이버시 침해 우려가 높게 나타났다. 논의된 결과를 바탕으로 관련 함의를 제시하였다.
이승은,양하나,손건우,박혜림,조정제,안현종,박증석,박용식 한국바이오칩학회 2015 BioChip Journal Vol.9 No.2
MicroRNAs (miRNAs) have been implicated in the regulation of physiological and pathophysiological processes such as vascular disease. Analysis of miRNA expression profiles in vascular disease provides new insights into the possible mechanisms underlying and therapeutic targets of disease. Acrolein is a highly reactive component of cigarette smoke, which has been implicated in the development of vascular disease. In this study, we investigated whether miRNAs play a role in the modulation of various gene expression responses to acrolein in human endothelial cells. We analyzed whole-genome miRNA and mRNA expression and found that acrolein induced differential expression of 151 miRNAs in endothelial cells. We further classified a number of target genes of the differentially expressed miRNAs. Among the differentially expressed miRNAs, miR-342-3p and 422a were upregulated, and the expression levels demonstrated a high inverse correlation with their targets, the vascular disease-related genes CLDN1 and PON1. Thus, integrating specific patterns of miRNA and mRNA levels may improve the diagnosis of vascular diseases induced byexposure to environmental pollutants such as acrolein.