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유산망아지에서 말herpesvirus 의 면역조직학적 및 immunogold 기법에 의한 전자현미경적 진단
신태균,이두식,진재광,임윤규,조길재 濟州大學校 農科大學 動物科學硏究所 1997 動物科學論叢 Vol.12 No.1
To investgate the causative Virus in aborbon of horse in Cheju, we examined the spleen of affected foal by immunohtochemistq and immunogold electron microscopy using EHV - specific antisera. Immunohistochemical staining revealed that a large number of EHV immunoreadive cells were deteded in the red pulp as well as in the sinudoidal cells in the affected spleen. By the immunogold electron microxopy(EM) of crude preparation of EHV culture, electron dense gold paricles were labelled along the envelope of amorphous virus parhcles with vanng sizes of 50-200nm. We suppose that immunohistochemical method is good for the diagnosis of tissues in EHV infections, and immunogold EM is recommended for the study of virus dia gnosis of fluid specimen as well as the ultrastustural study.
Hollow-Fiber LPME를 이용한 잔류농약 검출
조현우,이상섭,신윤지,장미애 경기대학교 기초과학연구소 2008 기초과학논문집 Vol.21 No.-
A method for the determination of trace amount of pesticides in aqueous samples using hollow fiber-liquid phase microextraction(HF-LPME) and capillary gas chromatograph/micro electron capture detector(GC/μ ECD) has been established. HF-LPME method has been optimized with respect to several experimental parameters including the effects of extraction solvent, extraction time and extraction temperature for the determination of pesticides from aqueous samples. The calibration curves for pesticides showed good linearity (above r²=0.98). The established method is feasible for the determination of trace amounts of pesticides in several aqueous sample. The limit of detection(LOD) and the limit of quantitation(LOQ) have been found to be 0.05~1.0 and 0.5~2.5 ng/mL, respectively. The established HF-LPME method for the analysis of pesticides from aqueous sample can be used for the determination of biological, food and environmental samples.
광주시 대기오염과 일별 사망의 상관성에 관한 시계열적 연구 : 1998~2001년
조용성,이종태,김윤신,현연주,박원석 대한보건협회 2003 대한보건연구 Vol.29 No.2
This study is performed to examine the relationship between air pollution exposure and mortality in Gwangju for the years of 19% - 2001. Daily counts of death were analyzed by general additive Poisson model, with adjustment for effects of seasonal trend, air temperature, humidity, and day of the week as confounders in a nonparametric approach. Daily death counts were associated with CO(current day), 03(6 day before), PMdcurrent day), NO₂(1 day before), SO₂(2 day before). Increase of 29.74 pg/m (interquartile range) in PM_(10)was associated with 1.4 % (95% CI = 0 % - 2.8 %) increase in the daily number of death. This effect was greater in children(1ess than 15 aged) and elderly(more than 66 aged). We concluded that Gwangju had 1 - 24 % increase in mortality in association with IQR in air pollutants. Daily variations in air pollution within the range currently occurring in Gwangju might have an adverse effect on daily mortality. These findings also support the hypothesis that air pollution, at levels below the current ambient air quality standards of Korea, is harmful to sensitive subjects, such as children or elderly.
조용성,이홍석,김윤신,이종태,박진수 한국환경과학회 2003 한국환경과학회지 Vol.12 No.6
To investigate the chemical characteristics of PM_(2.5) in Seoul, Korea, atmospheric particulate matters were collected using a PM_(10) dichotomous sampler including PM_(10) and PM_(2.5) inlet during the period of October 2000 to September 2001. The Inductively Coupled Plasma-Mass Spectromety (ICP-MS), Ion Chromatography (IC) methods were used to determine the concentration of both metal and ionic species. A statistical analysis was performed for the heavy metals data set using a principal component analysis (PCA) to derived important factors inherent in the interactions among the variables. The mean concentrations of ambient PM_(2.5) and PM_(10) were 24.47 and 45.27 ㎍/m³, respectively. PM2 5 masses also showed temporal variations both yearly and seasonally. The ratios of PM_(2.5)/PM_(10) was 0.54, which similar to the value of 0.60 in North America. Soil-related chemical components (such as Al, Ca, Fe, Si, and Mn) were abundant in PM_(10), while anthropogenic components (such as As, Cd, Cr, V, Zn and Pb) were abundant in PM_(2.5). Total water soluble ions constituted 30~50 % of PM_(2.5) mass, and sulfate, nitrate and ammonium were main components in water soluble ions. Reactive forms of NH₄^(+) were considered as NH₄NO₃ and (NH₄)₂SO₄ during the sampling periods. In the results of PCA for PM_(2.5), we identified three principal components. Major contribution to PM_(2.5) seemed to be soil, oil combustion, unidentified source. Further study, the detailed interpretation of these data will need efforts in order to identify emission sources.
서울시 대기오염과 일별 사망의 상관성에 관한 시계열적 연구 (1998~2001년)
조용성,이종태,김윤신,홍승철,김호,하은희,박혜숙,이보은 한국대기환경학회 2003 한국대기환경학회지 Vol.19 No.6
This study was performed to examine the relationship between air pollution exposure and mortality in Seoul for the years of 1998~2001I. Daily counts of death were analyzed by general additive Poisson model, with adjustment for effects of seasonal trend, air temperature, humidity, and day of the week as confounders in a nonparametric approach. Daily death counts were associated with CO (current day), 0₃(current day), PM_(10) (current day), NO₂, (1 day before), SO₂, (1 day before). Increase of 41.711㎍/㎥ (interquartile range) in PM_(10) was associated with 1.3% (95% CI = 0.7~1.9%) increase in the daily number of death. 0₃concentrations resulted in an increased risk of 1.3% for 23.86 ppb in all-aged mortality [RR = 1.013 (1.004-1.023)]. This effect was greater in children (less than 15 aged) and elderly (more than 65 aged). After ozone level exceeds 25 ppb, the dose-response relationship between mortality and ozone was almost linear. We concluded that Seoul had 1~5% increase in mortality in association with IQR (interquartile range) in air pollutants. Daily variations in air pollution within the range currently occurring in Seoul might have an adverse effect on daily mortality. These findings also support the hypothesis that air pollution, at levels below the current ambient air quality standards of Korea, is harmful to sensitive subjects, such as children or elderly.
Tetracyclic Isoquinoline유도체의 합성
조수동,정윤성,김정주,안철진,신동수 Institute of Genetic Engineering Changwon National 1998 Gene and Protein Vol.2 No.1
To develop new pharmacological charactization of isoquinoline derivatives, we have synthesized 3-benzyl-9,10-dimethoxy-6,7,11b,12-tetrahydropyridazino [4',5': 5,6] [1,4] -oxazino [3,4-а]isoqinolin-4(3H) -one. We have developed a convenient cyclization of novel isoquinoline derivatives starting from 3,4-dimethoxy phenethylamine using boron trifluoride diethyl etherate.
李允中,申秉澈,曺正吉,李東宣 成均館大學校 科學技術硏究所 1989 論文集 Vol.40 No.1
A simple, rapid and high sensitive method for the determination of prostaglandin F_2α (C_20H_34O_5) is described. 1-bromoacetylpyrene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. PG F_2α was derivatized quantiatively into fluorescent compound by reacting with 1-bromoacetylpyrene in the presence of 18-crown-6 in acetonitrile. The derivative was separated on a normal phase column in isocratic elution mode using the secondary mixture of chloroform and tetrahydrofuran as the mobile phase. The effluent was monitored by fluorometer. The calibration plots for the peak heights versus concentrations of PG F_2α were observed to he linear.