Anti-Inflammatory and Cytoprotective Effects of TMC-256C1 from Marine-Derived Fungus <i>Aspergillus</i> sp. SF-6354 via up-Regulation of Heme Oxygenase-1 in Murine Hippocampal and Microglial Cell Lines

Kim, Dong-Cheol,Cho, Kwang-Ho,Ko, Wonmin,Yoon, Chi-Su,Sohn, Jae Hak,Yim, Joung Han,Kim, Youn-Chul,Oh, Hyuncheol MDPI AG 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.4

<P>In the course of searching for bioactive secondary metabolites from marine fungi, TMC-256C1 was isolated from an ethyl acetate extract of the marine-derived fungus <I>Aspergillus</I> sp. SF6354. TMC-256C1 displayed anti-neuroinflammatory effect in BV2 microglial cells induced by lipopolysaccharides (LPS) as well as neuroprotective effect against glutamate-stimulated neurotoxicity in mouse hippocampal HT22 cells. TMC-256C1 was shown to develop a cellular resistance to oxidative damage caused by glutamate-induced cytotoxicity and reactive oxygen species (ROS) generation in HT22 cells, and suppress the inflammation process in LPS-stimulated BV2 cells. Furthermore, the neuroprotective and anti-neuroinflammatory activities of TMC-256C1 were associated with upregulated expression of heme oxygenase (HO)-1 and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in HT22 and BV2 cells. We also found that TMC-256C1 activated p38 mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways in HT22 and BV2 cells. These results demonstrated that TMC-256C1 activates HO-1 protein expression, probably by increasing nuclear Nrf2 levels via the activation of the p38 MAPK and PI3K/Akt pathways.</P>

조직학적 진단된 chronic necrotizing pulmonary aspergillosis 2예

김연숙,정숙인,기현균,김춘관,김신우,한정호,김성민,백경란,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

CNPA는 기존의 폐질환 및 폐절제술로 인해 국소방어기전에 저하가 있거나 비특이적 전신면역상태저하를 갖고 있는 환자들에게서 주로 발생하는 만성적인 공동성 폐질환이다. 현재까지 우리나라에서는 CNPA의 보고가 없는 상태이고, 저자들은 최근 조직학적으로 진단된 CNPA 2예를 경험하였기에 보고하는 바이다. 저자들이 경험한 CNPA 환자들은 만성알코올 중독 및 기관지확장증과 같은 기저질환과 폐절제술을 받은 병력이 있었다. Aspergillus가 폐실질을 침윤하고 있는 조직소견과 조직배양에서 Aspergillus가 검출되어 조직학적 진단이 가능하였고, amphotericin B의 정주요법 및 경구 itraconazole 투여와 폐절제술 등으로 성공적으로 치료되었다. 아직 많은 임상의들에게는 낯선 질환인 CNPA의 치료성적은 환자의 동반질환 및 CNPA 자체의 중증도, 진단과 치료 시작의 지연 등에 의해 크게 좌우되므로 만성적으로 진행하는 공동성 폐병변을 갖고 있는 환자에게서 CNPA를 감별하는 것이 중요하다. Chronic necrotizing pulmonary aspergillosis (CNPA) is a chronic cavitary form of pulmonary aspergillosis. Dozens of CNPA cases have been reported in patients with systemic immunologic dysfunction or altered local defense mechanism from preexisting pulmonary disease. Review of literatures revealed that no CNPA cases have been reported in Korea yet. We experienced two cases of CNPA proven by lung biopsy. A 53-year-old alcoholic male in poor nutritional state was admitted with generalized weakness and weight loss. Chest CT revealed a cavitary nodule surrounded with ground-glass attenuation. CT-guided fine needle aspiration and biopsy was done. The biopsy specimen demonstrated dichotomously branching septated hyphae consistent with those of Aspergillus sp. Another case was a 39-year-old man with bronchiectasis who was admitted with persistent hemoptysis. He had a past history of pulmonary tuberculosis. A parahilar lesion with intracavitary soft tissue mass was incidentally detected in high-resolution GT. Left lingular segmentectomy was done due to uncontrolled hemoptysis and CNPA was histologically diagnosed. Both patients were successfully treated with intravenous amphotericin B followed by oral itraconazole. Even though CNPA is unfamiliar to most clinicians, it should be included in differential diagnoses of chronic progressive cavitary pulmonary lesion, especially in patients with immunologic dysfunction.

두개골의 3차원 영상 분석을 위한 전산화단층촬영 방법의 비교 : 상층 두께가 3차원 영상의 계측에 미치는 영향

정호걸,김기덕,박혁,김동욱,정해조,김희중,유선국,김용욱,박창서 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.3

Purpose : To evaluate the quantitative accuracy of three-dimensional (3D) images by means of comparing distance measurements on the 3D images with direct measurements of dry human skull according to slice thickness and scanning modes. Materials and Mathods : An observer directly measured the distance of 21 line items between 12 orthodontic landmarks on the skull surface using a digital vernier caliper and each was repeated five times. The dry human skull was scanned with a Helical CT with various slice thickness (3, 5, 7 mm) and acquisition modes (Conventional and Helical). The same observer measured corresponding distance of the same items on reconstructed 3D images with the internal program of V-works 4.0 (Cybermed Inc., Seoul, Korea). The quantitative accuracy of distance measurements were statistically evaluated with Wilcoxons’ two-sample test. Results : 11 line items in Conventional 3 mm, 8 in Helical 3mm, 11 in Conventional 5 mm, 10 in Helical 5 mm, 5 in Conventional 7 mm and 9 in Helical 7 mm showed no statistically significant difference. Average difference between direct measurements and measurements on 3D CT images was within 2 mm in 19 line items of Conventional 3 mm, 20 of Helical 3 mm, 15 of Conventional 5 mm, 18 of Helical 5 mm, 11 of Conventional 7mm and 16 of Helical 7 mm. Conclusion : Considering image quality and patient’s exposure time, scanning protocol of Helical 5 mm is recommended for 3D image analysis of the skull in CT.

임신부 체중증가와 신생아 체중과의 관계

정효지,김은정,최봉순,최경호,신정자,윤성도 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.6

This study was carried out to find the factors which are related to the weight gain during pregnancy of women and infant birth weight. The information of the general characteristics and pregnancy outcomes of the 506 women who had a delivery during Jan to Dec. 1997 in a hospital at Taegu area were collected from the medical records. The results are as follows. The mean age of the subjects was 29 years old and the average prepregnancy weight was 52.75㎏. They gained 13.51㎏ of weight during the pregnancy. The weight gain during pregnancy was higher in prepregnancy BMI <20kg/m^(2), the infant weight was heavier in groups that had over 14kg of weight gain during the pregnancy than other groups. The prepregnancy BMI was negatively correlated to weight gain during pregnancy (r=0.2825), and positively correlated to number of pregnancy (r=0.2146), number of living delivery (r=0.1409), and infant weight (r=0.1250). The baby weight was positively correlated to weight gain during pregnancy (r=0.1392) and Apgar score(r=0.1627). The results showed that the prepregnancy BMI and weight gain during pregnancy may be the influential factors on the infant weight, thus we need to develop the specific nutritional management program according to the status of prepregnancy weight.

벼 잡종초기세대에서 이삭줄기 대유관속 수와 이삭 특성의 분리양상

김광호,안종국,서경인 建國大學校附設農業資源開發硏究所 2001 農資源開發論集 Vol.23 No.-

The segregation modes and the selection efficiency of large vascular bundle(LBV) number and panicle characteristics were measured in three hybrid populations of rice. F_1 plants of the three crosses showed different direction and degree of dominance in vascular bundle number and panicle characteristics. The panicle neck diameter(ND), number of primary branches(PB) and LVB showed a typical normal distribution in F_2. When F_2 plants were divided into two groups up culm length of 90cm, the distribution of LVB number and panicle characteristics were not different between two populations. The selected and non-selected F_3 populations showed no difference in distribution of LVB number and panicle characteristics. However, F_3 populations grouped by culm length of 90cm showed different distributions in LVB and 100-grain weight among six characteristics observed. In F_2 populations, ND showed positive correlationship with PB, LVB number and grain number per panicle(GN). ND was positively correlated with PB, LVB number, GN and panicle length in selected and non-selected F_3 populations.

嶺湖南地方 瘠薄林地의 오리나무와 아까시나무의 適地에 關한 硏究

金昌浩,鄭印九,吳正壽 동국대학교 농림과학연구소 1969 農林科學 論文集 Vol.3 No.-

The objectives of these studies were to investigate how every kinds of Black Locust and Alder influence on the growth of woods in the Yongnam and Honam district. The result of this study appeared as follows: 1. At the point of soil depth. the deeper the soil depth is, the better for Black Locust and Alder grows well regardless of it. 2. Black Locust shows good growth in loose soil but even in consistant soil Alder was excellently brought up. 3. Black Locust grows well in clay loam while Alder is appropriate to grow in sandy soil. 4. Black Locust requires an adequate soil depth so it grows well in gentle slope area but Alder grows well even in steep slope area which unproductive land enables to grow Alder satisfactorilly. 5. The constitution of cover vegetation could, to some extent, be attained in the superior forest land of Black Locust but it was impossible to make cover vegetatioe in that of Alder : It can therefore, be easily attained the open area between trees of Alder pure forest. 6. Black Locust and Alder are soil improving trees and so the content of effectivn phosphorus had a great effect on tree growth. 7. Black Loust forest land had more organic substance than Alder and this fact was closely connected with tree growth. 8. C. E. C is deeply concerned with the growth of black locust but less concernment related with Alder. 9. The suitable land for Black Locust. 1) A deep soil depth. 2) On less consistancy. 3) Contained much of P_2O_5 in soil. 4) Much of organic substance. 5) On a clay loam. 6) Forest land with greater C. E. C. 10. The suitable land for Alder : 1) Contained much of P_2O_5 in soil. 2) Much of organic substance. 3) With moderate consistancy and effective soil depth 4) on sandy soil. 11. It is assumed that Black Locust requires more soil conditions than Alder. 12. Haenam district of Chulanam-Do where severly damaged by disease was excluded from this survey study.

경쟁불안이 수영 선수들의 경기력에 미치는 영향

김원배,윤영길,현종호 서울대학교 체육연구소 2000 서울大學校 體育硏究所論集 Vol.21 No.2

The purpose of this study was to identify the effects of performance on the competitive anxiety in competitive situation. To achieve the aim of this study, the subjects were surveyed 172 swimming athletes and all athletes who selected the represent from each province was participated in Busan's Korea National Senior Games on 2000. The CSAI-2 Inventory developed from Martens et al(1990) was used to measure the athlete's psychological anxiety level, the method of survey was visited the athletic club school accommodation before 2 days ago in main events. The results was analysed by SPSS/PC and Mean/Standard Diviation were yielded in each factor. The independant t-test was used to know the difference of cognitive anxiety and physical anxiety on sex, athletic carrear, win experience after Middle School. On the basis of the results analyzed in this study, the conclusion were drawn as follows; 1.The difference of anxiety on sex was significantly appeared. The anxiety of girl players was higher than one of boy players. 2.The difference of anxiety on athletic carrear was significantly appeared. The anxiety of less carrear players was higher than one of more carrear players. 3.The difference of anxiety on win experience didn't significantly appeared.

도시거주자의 혈중 카드뮴 농도와 간 및 신장 기능과의 상관성 평가

김호현,임영욱,임종한,양지연,신동천 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.1

The possibility that liver and kidney function is adversely affected by current levels of environmental exposure to cadmium (Cd) as investigated in adult men and women in the general population in Seoul. From February to August in 2001, blood and morning spot urine samples were collected from 136 not occupationally exposed group (age range 20~75 years) at 4 survey sites throughout Seoul. Liver and kidney function parameters in serum and urine were examined by conventional methods. The questionnaire included factors, i.e. sex, age, smoking, alcohol, diet habit etc. The geometric mean values for Cd in blood (Cd-B) were 1.43 μg/l. It seemed prudent to conclude that liver and kidney function as not disturbed by the current environmental exposure to Cd in study.

치과주조용 저카라트 금합금의 시효경화

김형일,김종렬,안호경,장명익,설효정,김교한 대한치과기재학회 1997 대한치과재료학회지 Vol.24 No.2

The age-hardening behavior of a commercial dental casting low-carat gold alloy was investigated by means of differential thermal analysis, hardness test, optical microscopic observation and X-ray diffraction study. The following results were obtained. 1. By the isothermal aging of this alloy at 300∼400℃, the hardness increased markedly at the initial stage and continued to increased gradually for the time, and then decreased dractically after longer aging time. 2. The hardening was attributed to the formation of the metastable AuCu I' ordered phase from the α matrix. 3. The overaging with softening was attributed to the precipitation of the fine lamellar structure which was composed of the Ag-rich α1 and AuCu I ordered phases.

人性檢査를 利用한 MPDS 患者의 心理者 分析

金正浩,曺圭澄 慶北大學校 齒科大學 1984 慶北齒大論文集 Vol.1 No.1

A research scale of 48 items selected from MMPI by McCall et al. was used to compare psychologic aspects of 80 patients with MPDS to control I subjects and control Ⅱ subjects. For this, study subjects were selected 80 patients of MPDS who visited the Dept. of Dentistry, K.N.U.H., from December in 1982 to september in 1983. Control Ⅰsubjects were selected 80 outpatients without MPDS who required dental treatment from December in 1982 to september in 1983. And control Ⅱ subjects were selected 80 persons without MPDS who required no dental treatment and lived in Taegu. The results were as follow: In study subjects, somatic group and behavioral group are markedly higher than control (Ⅰ,Ⅱ) subjects. In somatic group and behavioral group, there was no significant difference between control I subjects and control Ⅱ subjects In somatic group and behavioral group, there was no significant difference between male and female in each subjects. In study subjects, general nature somatic category was higher than head, neck, throat and GIT somatic category. (P<0.05) In study subjects, somatic group and behavioral group gradully increased according to increase in number of MPDS symptom.