연부조직 육종에서 Cyclin D_(1) 유전자의 증폭에 대한 연구

유재훈,정동준,이정은,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The molecular mechanisms of the soft tissue sarcoma is not elucidated. In general, deregulations of the several genes such as oncogenes, antioncogenes, genes regulating apoptosis and cell cycle are known to take part in the carcinogenesis of most carcinomas. Moreover, deregulation of the cell cycle is one of the major carcinogenic mechanisms. The cyclin D_(1) is the most important gene regulating the transition, G_(1). to S, which is the most importan check point in cell cycle. The cyclin D_(1) gene amplification was reported in various carcinomas, but not in sarcomas. In this study, we studied the cyclin D_(1) expression in 10 liposarcomas, 5 rhabdomyosarcomas and 5 malignant fibrous histiocytomas by imunohistochedmistry, and real-time polymerase chain reaction. The results were as follows; 1. Immunohistochemically, liposarcoma and malignant fibrous histiocytomas expressed moderate degree of cyclin D_(1), however expression was noted in few rhabdo- myosarcomas. 2. The cycline D_(1) gene amplification was not different between normal and sarcomas(p>0.05). 3. There was no positive correlation between cyclin D_(1) expression by immunohistochemistry and cylin D(1)gene amplification. Above results suggest that cyclin D_(1) gene amplifacation does not contribute to the oncogenesis of the sarcomas, and cyclin D_(1) expression by immunohistochemistry does not correlate with the gene amplification. As the sample cases are limited in this study, forther studies with more samples are needed.

기술가치 평가모델의 비교 및 분석

兪日根,李在勳 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

Since the technology is one of the important factor for the development of countries or companies, evaluation of technology became essential for investment on venture company, technology trading and M&A. The appropriate evaluation of technology is so important yardstick of competence of business that, in this thesis, the theories and the present conditions of technology evaluation model in several institutes are compared and analysed and also the desirable direction for the future right model are suggested. Technology evaluation model is basically composed of three parts: cost-market-income approach method, technology factor method, real option method. We have to choose proper model among several methods because there are various technology evaluation methods and they have different merits and demerits. So that, due to some limits for each model, there is no complete one which can drive the right valuation by itself at one time. Thus, right valuation seems to be required that several different methods of each model should be applied and analysed at the same time and then decide the right answer that satisfies the suggested conditions.

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

Low doses of ionizing radiation suppress doxorubicin-induced senescence-like phenotypes by activation of ERK1/2 and suppression of p38 kinase in MCF7 human breast cancer cells

신재식,우상혁,이형찬,홍승우,유두현,홍석일,이왕재,이명석,진영우,안성관,진동훈,박인철 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

심상훈련이 스포츠 경기력에 미치는 효과 : 이론적 고찰 A Theoretical Observation

유진,김재훈,박봉섭 중앙대학교 스포츠과학연구소 2002 體育硏究 Vol.- No.17

An athlete must train his body and practice image training on an everyday basis in order to keep his competitive power in sports games and to improve it. This study is focused on the concept and theory of image training and its characteristics and actual effect along with the relevant case studies. Also, through thorough observations, the optimal psychological preparation for athletes to increase their competitive power in sports games was comprehensively studied. For image training to take effect and lead to the improvement in competitive power, both athletes and instructors should always try to find out ways to practice image training in a theoretical way, while working out to find out a psychological preparation method for each of the athlete, with more comprehensive point of view. Meanwhile, those who are interested in the psychological preparations to improve competitive power of athletes might need to take into consideration that most of what have been studied here are based on the cases of western athletes and were founded on the basis of their culture, and try to work out to provide a foundation on which to build an image training and psychological preparation strategy for Korean athletes.

일부 서울지역 실내.외 공기중 휘발성 유기화합물질(VOCs)의 농도조사

정제면,손종렬,유인성,변상훈 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Our communities have been exposed to polluted air. Polluted air could cause the serious injury such as respirational impediment, disease and so on, and the air pollution could be most serious problem in our life. Unfortunately, we didn't have consider these problems seriously, and we had make no solutions for these problems. Although the concentration of indoor and outdoor didn't show the high values, VOCs existed in indoor and outdoor environment. The indoor concentration was more higher concentration than the outdoor concentration, that indicated the indoor environment was more polluted. This study showed that the investigation of a continuous environment polluted were needed.

녹차 및 결명자 추출물의 교정용 브라켓과 치면 사이의 경계부에서 분리된 mutans streptococci에 대한 항균작용

임성훈,서정순,윤영주,김광원,유소영,김화숙,국중기,이병래,차종희,박재윤 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.5

본 연구는 치아우식증에 관련된 Mutans streptococci 표준균주 및 임상분리 균주의 성장억제를 유도할 수 있는 천연 생약추출물을 검색하기 위하여 실시되었다. 녹차추출물은 CHMC-2032를 사용하였으며, 결명자 추출물은 50% 에탄올을 이용하여 얻었다. 이들 추출물의 Mutans streptococci 표준균주 및 교정환자들에서 브라켓과 치면 사이의 경계부에서 분리된 각각 10 균주씩의 S. mutans 및 S. sobrinus에 대한 최소성장억제농도를 액체배지 희석법으로 구하였다. 그 결과 CHMC-2032의 S. mutans 및 S. sobrinus의 표준 균주, S. sobrinus의 대부분 임상분리 균주에 대한 최소성장억제농도는 5 mg/ml이었다. 그러나 결명자 추출물에 의한 S. mutans 및 S. sobrinus에 대한 세균 성장억제 효과는 미미하였다. 본 연구 결과 치아우식증의 예방적 측면에서 결명자차보다는 녹차를 마시는 것이 유리하며, CHMC-2032를 이용하여 구강양치용액을 제조하여 사용할 경우 교정환자를 포함하여 대부분의 사람에게서 치아우식증 예방 효과가 있으리고 추정된다. Mutans streptococci is the major causative factor in dental caries. Especially, orthodontic patients with fixed appliance are a risk group for dental caries. Because fixed appliances attached on teeth may change the environment of dental plaque, the enamel decalcification or dental caries around the bracket and band is a major side effect of orthodontic treatment. The aim of this study was to search plant extracts that have antimicrobial effect on mutans streptococci. Seed-extract of Casio tora were prepared with ethanol and CHMC-2032, the leaf-extracts from Camellia sinensis extract, was obtained extract, 2 type strains and 20 clinical isolates of mutans streptococci isolated from the interface between orthodontic brackets and tooth surfaces in the orthodontic patients were used in this study. The minimal inhibitory concentration of CHMC-2032 was 5 mg/ml on the S. mutans KCTC 3065, S. sobrinus KCTC 3088, and 8 clinical isolates of S. sobrinus. However, there was no antibacterial effect of seed-extract of C. tora on mutans streptococci. These data suggest that green tea may be more effective than the tea prepared from C. tora in the prevention of enamel decalcification or dental caries around brackets.

선형유도전자펌프내의 자기유체 유동에 관한 수치적 연구

최훈기,유근종,박태봉,은재정,장남영 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

Numerical analysis is performed for magnetic and MHD flow fields in Electro-Magnetic(EM) pump. A finite volume method is applied to solve magnetic field governing equations and the Navier-Stokes equations. Vector and scalar potential methods are adopted to obtain the electric and magnetic fields and the resulting Lorentz force in solving Maxwell equations. The magnetic field and velocity distributions are found to be influenced by the magnitude of the Reynolds number and the phase of applied electric current. Computational results indicate that the magnetic flux distribution with changing phase of electric current is characterized by a pair of counter-rotating closed loops. The velocity distribution of the flow field is influenced on the intensity of Lorentz force.

강원도 가칠봉의 식물자원

이재훈,장수길,유기억 강원대학교 환경연구소 2004 Journal of the Environment Vol.1 No.-

Plant resources of the Gachilbong were investigated from May, 2004 to October, 2004. Vascular plants of Gachilbong were composed of 86 families, 294 genera, 457 species 1 subspecies, 70 varieties and 10 formae, totaling 538 taxa. Twenty six species among the 538 species were Korean endemic, and the specific plants based on the distribution pattern appointed by Ministry of Environment were 96 taxa, and estimated 422 points. The naturalized plants were composed of 8 taxa. Life form spectra was H-D₄-R_(5)-e type, and the use of plant resources were composed edible 41.9%, pasture 35.7%, medicinal 32.9%, ornamental 10%, respectively.

혈당 측정에 있어서 반사광 비색법과 전기 화학 전극법의 비교

황유성,이도훈,우재근 충북대학교 의과대학 충북대학교 의학연구소 1995 忠北醫大學術誌 Vol.5 No.1

당뇨병환자에서 적절한 혈당을 유지하기 위한 목적으로 시험지봉 검사 방법이 1970년대 초 처음으로 개발된 이후 많은 자가 혈당기들이 고안되어 사용되고 있으며, 대부분이 반사광 비색법을 이용한 자가 혈당 측정기로 현재 국내에는 Glucometer(Ames), Glucoscan(Eiken), One TouchTRM Ⅱ(Lifescan), Accu-check Ⅲ(BM)등이 사용되고 있다. 저자들은 본원에서 이미 사용하고 있었던 반사광 비색법을 이용한 시험지봉으로 LIFESCAN사의 One TouchTM Ⅱ와 전기 화학 전극법을 이용한 Medisense사의 CompanionTM 2에 대하여 정밀도, 직선성, 비교방법과의 상관관계 및 혈액량과 헤마토크리트에 의한 영향, 관찰자간의 변이, 항응고제가 미치는 영향 등 측정에 영향을 미치는 여러 인자들에 대하여 평가를 시행하였다. 검사일내 정밀성(within day precision)은 두 기기 모두 5% 정도이었고, 검사일간 정밀성(day-to-day precision)은 CompanionTM 2가 3.14%∼6.53%, One touchTM Ⅱ가 4.69%∼8.13%의 변이계수를 보였다. 두 기기 모두 직선성이나 비교방법과의 상관관계가 우수하였다. CompanionTM 2와 One touchTM Ⅱ사이의 상관관계는 y=1.040x+21.679, r=0.9716이었다. 두 기기 모두 혈액량, 관찰자, 항응고제에 의해서는 영향을 받지 않았으며, 헤마토크리트에 의해서는 헤마토크리트가 낮을 때는 높은 혈당치를, 헤마토크리트가 높을 때는 낮은 혈당치를 나타냈다. The purpose of this study is to assess two monitors of blood glucose : One touch?? Ⅱ(Lifescan Inc.), which is using reagent strips by reflectance photometry and CompanionTM 2(Medisense Inc.), which is using electrochemical electrode method. Glucose was determined on capillary and venous blood samples from 70 adult diabetics by three different methodologies : One touchTM Ⅱ, CompanionTM 2 portable glucose meters (capillary) and the Hitachi 7150 analyzer(venous), which is using hexokinase method. In within-run precision studies for One touchTM Ⅱ and CompanionTM 2, coefficient variations (CVs, %) were 1.92∼5.09, 2.95∼4.79 at three different levels, respectively. In between-day percision studies for One touchTM Ⅱ and CompanionTM 2, CVs(%) were 4.69∼8.13, 3.14∼6.53 at three different levels, respectively. The linearity studies revealed r=0.9996 in One touch?? Ⅱ, r=0.9977 in CompanionTM 2, which were satisfactory. Correlation equation and coefficient for results by One touchTM Ⅱ and CompanionTM 2 vs those by hexokinase method(x) were : y=0.8087x-8.9566(r=0.9824) for One touchTM Ⅱ, y=0.8885x+4.4992(r=0.9807) for CompanionTM 2, which were satisfactory. The glucose levels were falsely high at low hematocrit concentration and falsely low at high hematocrit concentration in both monitors. The volume of blood applied to the test strips is not critical. We concluded that "One touchTM Ⅱ" using reagent strip by reflectance photometry and "Companion?? 2" using electrochemical electrode method is suitable for self monitoring of blood glucose.