각시붕어의 알발생과 부화자어

朴奉燮,朴奉憲 조선대학교 농업연구소 1986 農業硏究 Vol.1986 No.1

1986년 5월 15일 전라남도 무안군 몽탄면 양장리 파군교에 위치한 하천에서 채포한 각시붕어를 실험실 내에 설치된 수조에서 사육하던 중, 이들을 인공 수정시켜 알 발생 과정과 부화자어를 관찰한 결과를 요약하면 다음과 같다. 성숙란은 가느다랗게 늘어난 서양배 모양으로, 난경은 2.45∼2.75×1.45∼1.65㎜이며, 난황은 담황색이며, 불투명하고, 유구가 없는 분리 심성란이다. 사육 수온 17.0∼25.5℃ 범위에서 수정 후 39시간만에 처음으로 부화되어 나왔다. 부화 직후의 자어는 전장이 2.65∼2.70㎜(평균 2.68㎜)로서 난황의 앞부분은 등쪽과 배쪽으로 향하는 한쌍씩의 돌기를 형성하며, 근절수는 13∼14개이다. 부화 후13일째의 전장 6.5㎜인 자어는 황색소포가 두부 및 등쪽에 나타나며, 흑색소포도 처음으로 난황앞ㆍ등쪽ㆍ두부뒷쪽ㆍ미부의 제 7∼10 근절 사이에 나타난다. 부화 후 30일째의 전장 8.5㎜인 자어는 펄조개에서 나온 직후의 자어와 같이 난황이 완전히 흡수되어 있고, 등지느러미 앞부분에는 흑색소포가 증가한다. 부화 후 약 2개월째의 전장 14.4㎜인 치어는 D. Ⅲ11∼12, A. Ⅲ11∼12, P. 10V.7로 정수에 달하며, 몸 쪽에 비늘이 형성되고, 등지느러미 위에 본 종 치어기의 특징인 원형의 흑색 반점이 뚜렷해진다. A Study on Egg Development and Larvae of Pseudoperilampus uyekii MORI. Pseudoperilampus uyekii MORI is commonly distributed in the fresh waters of Korea. On May 15 in 1986, mature adults of rose bitterling were caught in the watercourse of Yang-Chang, Mong-Tan, Moo-An, Jeong-Nam, South Korea. The authors fertilized the eggs employing dry method in the laboratory on May 15 in 1986. Hatched larvae were reared in small aquariums at 17~25.5℃ (average around 21.2 ℃). Mollusks, Anodonia woodiana in the gill chamber of which, rose bitterling lay eggs were caught in order to study natural spawning of the rose bitterling in the same watercourse. The eggs of this species are not adhesive and demersal. The size of the eggs varies from 2.45 to 2.75mm in long diameter and 1.45 to 1.65mm in short diameter. The eggs are cylindrical in from when they are extruded from ovipositor, immediately after entering water, but they acquire their distinctive from of a greatly elongated pear. Hatching took place in ca. 39 hours after fertilization. The newly hatched larvae were 2.65~2.70mm in total length possessing yolk sac and 13~14 myotomes. Thirteen days after hatching, the prelarvae attained 6.5mm in total length, and the first mdanuphores appeared all the head, and the anterior part and sides of the yolk sac. One month after hatching, the postlarvae attained 8.5mm in total length and emerged from the gills of the mollusks. Then the yolk sac was completely resorbed. Two months after hatching, the rose bitterling attained 14.4mm in total length, and entered the fingerling period of life. All the rays already present were the D. III, 11-12, A. III, 11-12, P. 10, V.7, and a distinguishing feature is the presence of a black pigment spot in the lobe of the dorsal fin.

長城人工湖의 環境要因 및 植物性 Plankton 調査

朴奉燮,朴奉憲,梁聖鎬 조선대학교 농업연구소 1987 農業硏究 Vol.1987 No.1

장성호에 대한 환경적 요인이 Plankton의 생산과 박테리아의 분포에 어떠한 작용을 하고 있는가 즉 무생물이 생물에 미치는 영향을 알아보기 위하여 1986년 4월부터 12월까지 연4회(봄, 여름, 가을, 겨울)에 걸쳐 실시한 장성호의 조사 결과는 다음과 같다. 환경 요인으로서 장성호의 수온은 2차 조사(여름)에서 표층수가 28.5℃이었고, 수심 21.8m에서 수온 10℃로서 그 차이는 18.5℃이었고, 수심 6∼15m에서 thermoolin을 형성하고 있었다. 이어 2차 조사에서의 용존 산소는 수심 10∼15m에서 Oxycline을 이루고 있으며 이때 수심 15m에서 DO는 6.1㎎/ℓ이었다. 그리고 연평균 BOD는 0.79㎎/ℓ이었으며, 탁도는 연평균 5.6NTUs, pH는 7.2, 투명도는 연평균 1.75m, 전기전도도는 연평균 65.32V/㎝이었다. 또한 암모니아성 질소의 연평균치는 0.262m이었고, 인산은 0.103ppm으로 나타났다. 장성댐 저수지의 수질은 DO, pH, BOD, 투명도 등으로 볼 때 환경 기준 상수원수 1급에 해당하는 양호한 수질이다. Plankton 및 박테리아의 생물 조사로서는 식물성 Plankton에는 남조류 6속 7종, 규조류 10속 15종, 녹조류 18속 24종으로서 총 34속 46종을 조사하였고, 우점종은 Asterionella gracillima, Lyngbya Contorta, Melosira granulata, Melosira SP., Microcystics aeruginosa, Symedra Ulna, Euglena SP 등의 7종이었고, 이 중 Microcystics, Asterionella, Melosira의 3종은 Water bloom 현상과 관계가 깊다. 박테리아 조사는 일반 세균의 경우 가을에 평균 34.1×10?cells/gsoil, 암모니아산화 세균은 4.51×10?cells/gsoil, 아초산박테리아는 7.5×10?cells/gsoil, 곰팡이는 5.4×10³cells/gsoil, 방선균은 7.4×10⁴cells/gsoil이었다. In this study it was investigated how che environmental factors in the artificial lake of Chang-sung affect the birth and growth of plankton and the distribution of bacteria, that is, the effects of the environmental factors on the living things. The investigation has been procedured seasonally from April to December in 1986, and the results obtained are the followings. As for the environmental factors, the surface-water temperature turned out to be 28.5℃ in the second investigation (summertime), and the temperature in the depth of 2l.8m was 10℃, therefore its difference was 18.5℃, and thermocline was formed in the depth of 6~15m. Also the disolved oxygen in the second investigation formed oxycline in the depth of 10~15m. At this time the amount of DO was 6.1 mg/l in the depth of 15m, the annual mean BOD 0.79mg/l, the annual mean turbidity 5.6NTUs, the annual mean pH 7.2, the annual mean depth of clearness 1.75m, the annual mean conductivity of electricity 65.32 Micromohs/cm the annual mean value of ammonical nitrogen 0.262ppm, and phosphoric acid 0.103ppm. In view of DO, pH, BOD, depth of clearness, the lake of Chang-sung had the good purity of water for the service water source in the light or environmental standard. In this study living things like plankton and bacteria were investigated. A total of 34 genuses 46 species of the Phytoplankton was classified into 6genuses 7species of Cyanophyta, 10 genuses 15 species of Bacteriophyta, and 18 genuses 24 species of Among them the dominant species were Asterionella gracillima, Lyngbya contorta, Melosira granulata, Melosira Sp, Microcystics aeruginosa, Synedra, ulna, Euglena sp, and Microcystics, Asterionella, Melosira were closely related with the phenomenon of water bloom. In the investigation of bacteria, the annual mean bacteria was 34.1 × 10^6cells/g soil, ammonia oxydative bacteria 1.51×l0^5cells/g soil, acetobacteria 7.5×10^5cells/g soil, fungus 5.4 × 10^3cells/g soil, and actinomycetes 7.4 × 10^cells/g soil.

수영이 정신지체아의 체력향상과 Dopamine의 농도에 미치는 효과

박봉섭 한국특수교육총연합회 2004 현장특수교육연구보고서 Vol.- No.-

龍溪山一帶 山火跡地의 初期植生과 二次遷移에 관한 硏究

朴奉燮,鄭洪來 조선대학교 농업연구소 1986 農業硏究 Vol.1986 No.1

본 調査는 由火跡地의 初期植生 二次遷移의 初期段階를 究明하기 위해서 실시하였고 그 結果는 다음과 같다. ① 由火跡地인 龍?山 一帶에서 調査된 維管動植物은 總 97科, 262屬, 391種, 64變種이고 調査地 內에서 出現된 植物은 62科, 139屬, 149種, 12變種이었다. 標本抽出한 方形區 內에서 出現된 種數는 一次年度(1984)의 由火地에서 31種, 5次年度(1978)의 由火地에서 71種으로 5次年度가 多樣하게 나타났다. ② 調査地에서의 優点種順位는 김의털 SDR₄:80.95, 억새 SDR₄:78.55, 조록싸리 SDR₄: 72.53, 털진달래 SDR₄:64.90 順이었고 調査地의 生活型 組成은 H-D?-R?-e型으로 나타났다. ③ 遷移度(DS)는 B₄池所가 DS=1422로 가장 높았고 B₁, B₂, B₃地所에서는 DS=913∼965로 類似하였다. 種의 多樣度는 B₃地所가 ?=1.2158로 가장 높고 B₄地所가 ?:0.9925로 낮게 나타났다. 均等性指數(e)는 e:0.7393∼0.7673으로 각 池所間에 類似한 값을 나타내어 出現된 種數가 비슷하며 種이 비교적 均等하게 分布하고 있음을 보여 주었다. ④ 土壤 內의 pH, organic matter, N, P, K, Ca의 함량은 表土層(0∼5㎝)이 6∼30㎝層보다 전반적으로 그 含量이 높게 나타나고 있어 非山火地의 狀態로 回復되어 가는 傾向이었다. 따라서 山火 후의 植生回復過程과 土壤成分의 變化 사이에는 密接한 關聯이 있음을 알 수 있었다. ⑤ 耐火性이 강한 떡갈나무, 졸참나무, 때죽나무, 털진달래 조록싸리 등은 뿌리와 줄기에서 再生하고 있었다. ⑥ 소나무 林地는 山火 후 植生回復이 늦어지고 種類組成도 貧弱하였고, 荒廢化 되기 쉬운 地域으로 調査되었다. This paper is a part of sereies of the investigation of early vegetation, secondary succession and soil in the forest fire area. The forest fire had occurred in April, 1978 and February, 1984. The site is located in the north eastern facing slope at about 1,200 meters from peak of Mt. Yongkae (626 meters high). The investigation conducted nine times from August 11, 1983, to August 16, 1984, The results are summarized as follows : 1. The floristic composition in the investigated area was 97 families, 262 genera, 391 species and 64 varieties. Among them, the floristic compositions of burned area were 62 families, 139 genera, 149 species and 42 varieties, 31 species ill first year and 91 species in the fifth year in the sampling sites have been found. 2. Festuca, Miscanthus sinensis var. Purpurascens Lespedeza Maximowiczii, and Rhododendron mucronulatiwn var. ciliatwn were dominant species in the burned area and biological spectra showed the H-D_1-R_5-e type in the burned areas both in the year of 1978 and 1984. 3. The degrees of succession (D3) of B_4 stand was comparatively the highest than those of the burned areas and B_1, B_2 and B_3 stands were most similar, and species diversity index(H) of B_3 stand was comparatively highest than those of the other stands and B_4 stand was decreased among the sampling sites. It showed that the evenness index between B_1, B_2, B_3 and B_4 stands were most similar among the sampling sites. 4. Compared with those of 0-5cm depth, the soil properties of pH, organic matter, nitrogen, phosphorus, and calcium in 6-30cm depth were decreased. It was assumed that these results were due to the forest fire. 5. Quercus dentata, Quercus serrata, Styrax japonica, Rhododendron mucronulatun var. Ciliatwn and Lespedeza maximowiczii which are fire-resistant sprouted again in the roots and the trunks. 6. After-forest fire, pinus densiflora forest were late in vegelation recovery, were poor in species composition, and were regarded as easily derastated regions.

魚類全身의 透視標本製作에 관한 比較硏究 : 홍어, 참서대 및 참가자미를 中心으로

朴奉燮,吳永男 조선대학교 농업연구소 1980 農業硏究 Vol.1980 No.1

본인은 動物體를 解剖하지 않고 魚類全身을 그대로 保存液속에서 透明하게 만들어 다음과 같은 成果를 얻었다. 1. 不透明한 生物體의 內部構造를 立體論으로 透視할 수 있게 되었다. 2. 軟欺性 魚類인 홍어(Raja kenojei Muller et Benle), 참서대(Areliscus joyneri Gunther) 및 참가자미(Pseudopleuronectes herznsteini Jordan et Snyder)등의 骨? 分布狀態를 完全하게 透視한 수 있게 되었다. 3. 珍標本을 製作한여 視聽覺敎育에 活用할 수 있게 되엇다. By making the whole bodies of fish transparent in the preserving liquids without dissecting them, the investigator reached the following conclusions: 1. It enabled to see through the inner structures of turbid bodies ,of fish minutely and in three dimensions: 2. It enabled to see through completely the distribution of bone structures of Raja kenojei Muller et Henle, Areliscus joyneri Gunther and pseudopleu.ronectes herzensteini Jordan et Snyder ; 3. Manufacturing reare specimens based on this technique, the way of utilizing them to audiovisual education was developed.

우리나라 主要 水稻品種의 耐蟲性에 관한 硏究 : 浮塵子類를 中心으로 on the resistance to the Planthoppers

朴奉燮,金奎眞 조선대학교 농업연구소 1978 農業硏究 Vol.1978 No.1

本昭究는 稱作害蟲으로서 問題化되고 있는 浮塵子類中 벼별구, 흰등멸구, 끝동매미충에 對한 抵抗性程度를 밝히고자 全國 各道 ?勵品種을 包含한 主要品種 40個와 最近에 育成된 優良系統 6個를 公試하여 2~3齡의 若蟲 接種 耐性의 反應을 檢討하고 벼멸구에 對한 抵抗性品種과 感受性品種의 親和性을 調査한바 다음과 같은 結果를 얻었다. 1. 抵抗性品種 Mudgo와 感受性品種 萬頃에서의 벼멸구 飼育日數에 따른 蟲休의 增加量은 接種當時에 比하여 接種後 l5日에 Mudgo에서는 2倍程度였으나 萬頃에서는 3倍以上의 增加量을 보였다. 2. 感受性品種(萬頃)과 抵抗性品種(Mudgo)에서의 벼멸구 飼育日數別 生蟲數와 死蟲數 比較에서 接種後 15日에서 Mudgo 74.0% 萬頃 31.2%로서현저한差異가 있었다. 3. 벼멸구에 對한 抵抗性 檢定에서는 優良系統의 KR-108-243-l, HR 529-41-3-2가 高位抵抗性 反應을 보였고 基他 大部分의 品種들이 感受性反應을 보였으나 赤수, 사도미노리, IR 24, IR 8等이 中度感受性 反應이었다. 4. 횐등멸구에 對한 反應에서는 赤是 優良系統中의 KR 108-243-1, KR 109-154-2, HR 529-45-3-2가 高位抵抗性이었고 ?勵品種 및 優良品種은 感受性이었으며, 水原82號, 統一, IR8, 八錦, 裡里309, 湖光, 中國31號 等이 中度受性反應을 보였다. 5. 끝매미충에 對한 檢定에서는 優良系統의 KR108-234-l, HR 529-135-1이 抵抗性이었고, 벼멸구, 흰등멸구에 抵抗性인 Mudgo가 感受性이었고, 統一, 水原82號, IR24, 密成, 中國31號가 中度感受性反應이었으며, 其他 品種들은 高位感受性이었다. 6. 本試驗의 結果 KR 105-243-1이 供試된 浮塵子類에 抵抗性反應이었으며, 感受性이기는 하나 比較的 벼멸구, 횐등멸구에 IR8, 벼멸구, 끝동매미충에 IR24의 Indica系統들이 흰등멸구, 끝동매미충에 統一, 水原82號가 中度感受性이었다. The objective of this study to clarify the varietal resistance to green leaf-and plantho-pper and the evaluate the nature of the resistance to the insects in connection with the Antibiosis. This study was investigated the reaction of, the varieties to insects with 46 rice varieties including recommended varieties of Korea, At seedling stage they were in fested with second or third inster nymphs. The results were as follows. 1. The body weight of Brown planthopper was increased more than 3 times' of the weight obtained before caging on susceptible rice variety Mankyung at 15 day after infestation but only 2 times on resistance mudgo. 2. The mortaliy of the Brown plant hopper was significatly difference between resistance and susceptible rice plant, showing 74 percent in resistance Mudgo while 31.2 percents in susceptible Menkyung at 15 days after caging. 3. Most of the rice varieties tested showed highly susceptible reaction to Brown plant-hopper except Mudgo, HR108-243-1 and HR 529-41-3-2 as resistance, and Aka-moch, Satominori, IR 24 and IR 8 as moderataly susceptible. 4. In the test of varietal resistance to white bached planthbpper Mudgo, KR108-243-1, KR109-154-2 and HR 529-45-3-2 were resistance, and Suweon 82, Tongil, IR 8 Palkeum, Iri 309, Hokwang and chukoku #31 were moderatly susceptible. 5. Most of the varieties tested were observed highly resistance to green leafhopper, but Tongil, Suweon 82. IR 24, Milsung and Chuhoku #31 were moderatly susceptible. 6. The new bred lines HR 108-243-1 are multiple resistance to Brown planthopper, green leafhopper and white backed planthopper, and some others showed moderatly susceptible as follows. IR 8 : Brown planthopper, white backed planthopper. IR24 : Brown planthopper, green leafhopper. Tongil : Suweon#82, Chukoku#:31. White backed planthopper, Green leafhopper.

테니스 스포츠상해 및 예방에 관한 연구

박봉섭,강형숙 한국스포츠리서치 2007 한국 스포츠 리서치 Vol.18 No.6

動物의 冬眠이 血液像에 미치는 影響 : 미꾸리 (Misgurnus anguillicaudatus), 두렁허리 (Fluta alba), 개구리 (Rana nigromaculata nigromaculata), 까치살모사(Agkistrodon halys halys), 집박쥐 (Pipistrellus abramus) 의 冬眠時 血液의 血球値 血色素値 및 血液水分含量을 中心으로 Effects of the Hibernation on the Blood, Pictures with Special Reference to the Corpuscles, Hemoglobin Content, and Water Volume of the Blood during Hibernation in the Loaches (Misgurnus anguillicaudatus ), Eels(Fluta alba), Frogs (Rana nigromaculata),

朴奉燮 朝鮮大學校 文理科大學 1973 論文集 Vol.1 No.-

With respect to the counts of red blood corpuscle and white blood corpuscle, the content of hemoglobin, and the content of water in blood during hibernation of the pisces, amphibia, reptilia, and mammalia, the following results were obtained: Loaches (Misgurnus anguillicaudatus) and eels(Fluta alba) began hibernation at 5-6℃ of water temperature ; frogs (Rana nigromaculata nigromaculata) and snakes (Agkistradon halys halys) at 5-6.5℃ of air temperature; and bats (Pipistrellus abranus)at 5.7℃ . The loaches and eels started complete hibernation at 5-6.5℃ of water temperature; the temperature of shelter beneath the earth during hibernation was 10.62℃ (at 20 cm on November)and 11.29℃ (at 30 cm on November) respectively, while bats on the earth showed 38℃ of the body temperature. The temperature of water and air when these animals began awakening was at 10℃ for loaches and at 10-15℃ for eels repectively; at 8.8-10℃ for frogs and snakes, and bats with 14.3℃ of body temperature at 11.4℃ in air temperature. Arousal from hibernation was: at 25-26℃ of water temperature for loaches; at 23-28℃ for eels; at 25-27℃ for frogs and snakes; and at 11.4-11.9℃ (in air temperature) for bats with 36-37℃ ofthe body temperature. The erythrocyte count, leucocyte count, and hemoglobin content of hibernation, awakening, creeping, normal activity, and inactivity resulted in: the values of these gradually increased in normal activity. In other hands, the content of water in the blood decreased until creeping period. It may be concluded that the hibernation depends on the external environmental factors which are PH of water, soil, water, and air temperature, and that results the changes of blood pictures.

Skeletal Muscle Troponin I (TnI) in Animal Fat Tissues to Be Used as Biomarker for the Identification of Fat Adulteration

박봉섭,오영경,김민진,심원보 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.6

In this study, the existence of skeletal muscle troponin I (smTnI), well-known as a muscle protein in fat tissues, and theutilization of smTnI as a biomarker for the identification of fat adulteration were investigated. A commercial antibody(ab97427) specific to all of animals smTnI was used in this study. Fat and meat samples (cooked and non-cooked) of porkand beef, and chicken considered as representative meats were well minced and extracted by heating and non-heating meth-ods, and the extracts from fat and meat tissues were probed by the antibody used in both enzyme-linked immunosorbentassay (ELISA) and immunoblot. The antibody exhibited a strong reaction to all meat and fat extracts in ELISA test. On theother hand, the results of immunoblot analsis revealed a 23 kDa high intensity band corresponding to the molecular weightof smTnI (23786 Da). These results demonstrate that the existence of smTnI in all animal fat tissues. Since there are mon-oclonal antibodies specific to each species smTnI, smTnI in fat tissues could be used as a biomarker to identify or determineanimal species adulterated in meat products. Therefore, an analytical method to identify fraudulent fat adulteration can bedeveloped with an antibody specific to each species smTnI.

수영이 정신지체인의 체력요인과 도파민 농도에 미치는 영향

박봉섭,변재종,이창규 한국특수체육학회 2004 한국특수체육학회지 Vol.12 No.2

The purpose of this study was to find out the effects of swimming exercise programs on muscular function, cardiorespiratory function, agility, flexibility, balance, and dopamine concentration in the mentally retarded. The subjects were 5 mentally retarded students of high school for special education. The physical fitness factor and dopamine concentration were measured before and after 22-week swimming exercise(2 times/week, 60minutes/session). The statistical procedures used to the analysis were paired t-test. The results were as follows; First, the increase of physical fitness factors was shown in the cardiorespiratory function and flexibility. Second, the increase of dopamine concentration was shown. It could be concluded in this study that swimming exercise program had significant effects on the improvement of physical fitness and dopamine concentration of the mentally retarded.