미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자에서 혈장 Endothelin의 변화

서예경,정상원,남직화,신병호,전종민,김정국,김동희,하승우,김보완 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 혈관내피세포에서 생성되는 endothelin(ET)는 강력한 혈관수축작용을 가진 펩타이드로 당뇨병 환자에서 혈관내피세포의 손상여부에 따라 혈중 농도가 증가할 수 있으며 혈관합병증과 관련이 있다는 보고가 있다. 그러나. 혈중 ET치의 상승과 당뇨병성 합병증의 상관성에 대해서는 논란이 많다. 이에 저자들은 인슐린 비의존성 당뇨병 환자에서 미세알부민뇨의 동반유무에 따른 혈중 ET치의 변화 및 혈장 ET치와 요중 알부민 배설률과의 상관성을 검토하였다. 대상 및 방법 : 인슐린 비의존성 당뇨병 환자중 미세알부민뇨를 보인 22명과 정상알부민뇨를 보인 43명을 대상으로 하였으며 혈장 ET를 측정하여 요중 알부민 배설률, 당뇨병성 망막증 및 당뇨병 관련인자들과의 관계를 비교하였다. 결과 : 당뇨병성 망막증이 합병된 군에서 망막증이 합병되지 않은 군에 비하여 혈장 ET치가 유의하게 높았던 반면 (각각 1.9±0.66, 1.5±0.76pg/㎖, p<0.05) 미세알부민뇨를 보인 군과 정상알부민뇨를 보인 군사이에 혈장 ET치는 통계학적으로 유의한 차이가 없었다(각각 1.9±1.14, 1.7±0.69pg/㎖). 또한 요중 알부민 배설률과 혈장 ET치 사이에 유의한 상관관계는 없었으며 미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자군에서 혈장 ET치는 당뇨병의 관련인자들과도 유의한 상관관계가 없었다. 결론 : 인슐린 비의존 당뇨병 환자에서 혈장 ET치가 초기 당뇨병성 신증의 합병여부나 미세알부민뇨의 정도를 반영하기는 어려울 것임을 시사한다. Background : Endothelin(ET), a potent vasoconstritor peptide released from vascular endothelial cells, is known to be increased in diabetic patients with vascular damage and related with diabetic angiopathy. However, whether plasma ET levels may be related to diabetic complications is controversial. We studied the change of ET levels in non-insulin dependent diabetics with microalbuminuria and relation between ET and urinary albumin excretion rate. Methods : Plasma ET levels of 22 non-insulin dependent diabetics with microalbuminuria and 43 patients with normoalbuminuria were measured and relations of plasma ET to diabetic complications such as urinary albumin excretion rate, retinopathy and other associated factors were studied. Results : Plasma ET levels were significantly higher in patients with diabetic retinopathy than those without retinopathy(1.9 ± 0.66 vs 1.5 ± 0.76 pg/㎖. p<0.05). The difference, however, between diabetics with and without microalbuminuria was not significant(1.9 ± 1.14 vs 1.7 ± 0.69 pg/㎖). There were no correlations of plasma ET to urinary albumin excretion rate and to the other associated factors in non-insulin dependent diabetics. Conclusion : These results suggest that plasma ET levels may not seem to act as a predictor of diabetic nephropathy or microalbuminuria in non-insulin dependent diabetics.

7,12-Dimethylbenz(a)anthracene(DMBA)에 의해 유발된 생쥐 피부종양의 특성

장예진,한상철,김완종 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.2

Skin tumor of hairless mouse (Mus musculus) induced by 7,12-dimethylbenz(a)anthracene (DMBA) was observed by light and electron microscopes. On histologic examination, it was revealed that the tumor type was the squamous cell carcinoma. The skin tumor tissue had the thick epidermis and was more keratinized than the case of normal skin. Electron microscopy of the tumor cells, in comparison with normal cells, showed a number of cytoplasmic processes and a cytoplasmic bridge between adjacent cells. Also, the ratio of heterochromatin to euchromatin was high in the tumor cell. There were plentiful free ribosomes and numerous mitochondria in the cytoplasm, suggesting the use of for rapid proliferation. At the dermal-epidermal junction, the basal lamina of tumor cells showed the intermittent discontinuities, through which their cytoplasmic processes penetrate, indicating invasion or metastasis of the tumor cells.

Thermoplasma acidophilum의 20S Proteasome에 의한 ATPase-활성적 단백질분해에 관한 연구

우기민,장예진,조만희,김창세,김완종,조성호,이상한 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

The eukaryotic 26S proteasome is an ATP/ubiquitin-dependent proteolytic complex consisting of the 20S core particle and 19S ATPase complex. However, because of its complexity and unstable properties, this study was carried out to present more simple and stable model for the ATP-activated proteolytic complex in prokaryotes which can take the place of the eukaryotic 26S proteasome. For this purpose, recombinant Thermoplasma 20S proteasome (T20S) and Methanococcus MS4, a sequence homolog of one ATPase subunit in the 19S ATPase complex, were successfully isolated from Escherichia coli (E. coli). The α and β subunits of T20S expressed in E. coli could assemble for themselves, and showed the peptide-hydrolyzing activity. Whereas both T20S and R20S (the 20S complex from rabbit skeletal muscle) had the highest peptidase activity against Suc-LLVY-AMC, a good substrate for chymotrypsin-like peptidase activity, the specific activity of T20S was slightly lower than that of R20S. In addition, several reagents such as KCI, SDS, and ovalbumin were shown to have different effects on the peptidase activities between T20S and R20S. When the ATPase activity of the purified MS4 were assayed , the Km for ATP was about 0.5 mM, and casein could stimulate the activity more than 2 fold without the change in Km. This result implicates that protein-activated ATPase may induce the conformational change of casein, and therefore suggests that MS4 ATPase may activate the proteolytic activity of the 20S proteasome via accelerating the recognition and translocation of the protein substrates.

Streptozotocin-당뇨병 백서에서 당뇨병 이환기간에 따른 대동맥의 이완능 저하 및 Oxygen-Free Radical에 의한 이완능의 손상정도

김정국,김신우,김수동,서예경,하승우,김보완,백운이,권삼,강승완 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

목적: 당뇨병의 이환기간이 혈관의 내피세포 의존성 및 비의존성 이완능의 저하에 미치는 영향과 이환기간에 따라 oxygen-free radical(OFR)에 의한 동맥이완능의 손상정도에 차이가 있는지를 알아보고자 하였다. 방법: Streptozotocin(STZ)으로 당뇨병이 유도된 3 주 및 10주째의 백서군과 연령이 비슷한 정상 대조백서군의 적출 대동맥환을 norepinephrine으로 수축시킨 상태에서 내피세포 의존성 이완제인 acetylcholine(ACh) 및 비의존성 이완제인 nitroglycerine(NTG)에 대한 이완능을 즉정하여 비교 관찬하였다. 또한 xanthine과 xanthine oxidase의 혼합으로 생성된 H₂O₂에 대동맥환을 노출시킨뒤 역시 ACh과 NTG에 대한 이완능의 손상정도를 비교하였다. 결과: 3주및 10주째 당뇨병 백서의 대동맥환은 모두 대조군 백서의 대동맥환에 비해 ACh에 대한 이완능이 유의하게 감소하였으며 10주째 당뇨병 백서군에서의 ACh에대한 이완능의 저하는 3 주째 당뇨병 백서군에 비해 유의하게 심하였다.H₂O₂에 노출시킨 뒤 ACh에대한 이완능은 3주 및 10주 째 당뇨병 백서군에서 모두 대조군에 비해 유의하게 심한 손상을 보였으나 3주와 10주째 당뇨병 백서군 사이의 손상정도의 차이가 통계학적으로 유의하지는 않았다. 한편 3주 및 10주째 당뇨병 백서군 모두 NTH에 대한 대동맥환의 이완능은 대조 백서군과 별다른 차이가 없었으며 또 H₂O₂에 노출시킨 뒤에도 NTG에 대한 이완능의 손상은 관찰되지 않았다. 결론: STZ-당뇨병백서에서 대동맥의 이완능은 당뇨병에 이환된지 3 주째에 이미 ACh에 대한 내피세포 의존성 이완능이 선택적으로 저하되며 당뇨병의 이환기간이 길수록 이완능의 저하가 심하였고, OFR에 의한 동맥 이완능의 손상도 내피세포 의존성 이완능에만 선택적으로 나타나나 이환기간에 따라 OFR에 의한 이완능 손상정도에는 뚜렷한 차이가 없음을 시사하고 있다. Objectives: Diabetes mellitus produces histological evidence of endothelial as well as changes in vascular function in experimental animal. Oxygen free radicals interfere with of destroy endothelium-dependent relaxation in normal and diabetic blood vessels. The present study was investigated whether the duration of diabetic state can affect the severity of impairment in endothelium-dependent and/or independent relaxation and the sensitivity to oxygen free fadical (OFR)-induced damage in diabetic rat vasculature. Methods: The relaxative responses of aortic ring precontracted with norepinephrine in vitro to cumulative concentrations of acetylcholine (ACh) and nitroglycerine (NTG) were conducted in 3- and 10- week streptozotocin (STZ)-diabetic rats and agematched controls. After exposure to H₂O₂generated by mixing xanthine and xanthine oxidase, the degree of impairment in relaxative responses to ACh and NTG were also compared between 3- and 10-week diabetic rats. Results: The relaxative responses of aortic rings produced by cumulative concentrations of ACh were significantly lower in both 3- and 10-week diabetic rats than in the age-matched controls. The impairments of relaxative responses to ACh in 10-week diabetic rats were significantly greater than those in 3-week ones. After H₂O₂ exposure, the impairments of relaxative responses to ACh in 3- and 10-week diabetic aortas was also significantly greater than those in the control in the control ones. However, the severity of H₂O₂-induced damage in 10-week diabetic aortas was not significantly different than that in 3-week ones. In contrast, aortic rings of 3- and 10-week diabetic rats could still be fully relaxed by NTG, even after exposure to H₂O₂. Conclusion: These findings suggest that in SRZ-diabetic rat aortas selective impairment of endothelium-dependent relaxation to Ach can be found early even over a 3-week period after the induction of diabetes and the impairment may be greater in those with the longer duration of the diabetic state and that the impairment of the vascular relaxation by OFR is also selective to endothelium-dependent response but the sensitivity of OFR-induced damage may not be related to the duration of the diabetic state.

Layered double hydroxide nanomaterials for bifunctional ORR/OER electro-catalyst

Ye Wanli,Kim Tae Woo,Park Dae-Hwan 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.6

As climate change continues to intensify global warming, energy security has become a major concern to the international community. Given the high energy density, portability, and environmental friendliness of energy conversion and storage devices such as fuel cells, they are being used as clean and effi cient energy sources and widely researched and applied. In a series of nanoscale materials for the oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) at the electrode of the fuel cells, they are considered to be promising non-precious metal-based electricity catalysts and there has still been a great opportunity for further development and optimization. One of the ceramic nanomaterials, layered double hydroxides (LDHs) have their unique two-dimensional layered structure, LDHs and their nano-hybrids also have high activity, low cost, long life, and are environmentally friendly. This article provides a comprehensive introduction to the structure of LDH, the latest research progress on the ORR activity and the ORR/OER dual function of the electrochemical catalysts with LDH nanomaterials.

S-445 Outcomes Analysis of Surgical and Medical Treatments for Patients with Primary Aldosteronism

( Ye Seul Yang ),( Kyeong Seon Park ),( Jung Hee Kim ),( A Ram Hong ),( Dong-hwa Lee ),( Min Kyong Moon ),( Sung Hee Choi ),( Chan Soo Shin ),( Sang Wan Kim ),( Seong Yeon Kim ) 대한내과학회 2016 대한내과학회 추계학술발표논문집 Vol.2016 No.1

Objective:?Patients with aldosterone-producing adenomas are treated using surgery, and patients with idiopathic hyperaldosteronism receive medical treatment using mineralocorticoid receptor antagonists (MRA). However, the outcomes of surgical and medical treatment for primary aldosteronism (PA) remain unclear. Therefore, we compared the outcomes of surgical and medical treatment for PA and aimed to identify a specific subgroup that can benefit from medical treatment.?Design:?Retrospective observational study.?Methods:?We identified 269 patients with PA (unilateral excess: 221 cases; bilateral excess: 48 cases) from 2000-2015 at the Seoul National University Hospital and two other tertiary centers. The main outcomes were the amelioration of hypertension and hypokalemia.?Results:?Treatment improved hypertension in the surgical treatment group (78.2%) and the medical treatment group (55.6%) (P?<0.001). At the last follow-up, hypokalemia was normalized in the surgical treatment group (97.1%) and the medical treatment group (93.7%;?P?= 0.046). Among patients with unilateral aldosterone excess, surgery provided advantages in resolving hypertension and normalizing hypokalemia without worsening renal function. Among patients who were >60 years old, surgical and medical treatment provided similar amelioration of hypokalemia and hypertension (71.4% vs. 56.5%,?P?= 0.243; 88.6% vs. 100%, p=0.244; respectively). The surgical treatment group exhibited lower postoperative eGFR and higher serum potassium levels, compared to the medical treatment group.?Conclusions:?Surgical treatment provided better hypertension and hypokalemia outcomes among patients with PA, compared to medical treatment. However, MRAs may be appropriate for elderly patients with impaired renal function.

Biodistribution of poly clustered superparamagnetic iron oxide nanoparticle labeled mesenchymal stem cells in aminoglycoside induced ototoxic mouse model

Ye Ji Ahn,Wan Su Yun,최진실,김우철,Su Hoon Lee,Dong Jun Park,박정은,Jaehong Key,서영준 대한의용생체공학회 2021 Biomedical Engineering Letters (BMEL) Vol.11 No.1

Recently, application of stem cell therapy in regenerative medicine has become an active field of study. Mesenchymal stem cells (MSCs) are known to have a strong ability for homing. MSCs labeled with superparamagnetic iron oxide nanoparticles (SPIONs) exhibit enhanced homing due to magnetic attraction. We have designed a SPION that has a cluster core of iron oxide-based nanoparticles coated with PLGA-Cy5.5. We optimized the nanoparticles for internalization to enable the transport of PCS nanoparticles through endocytosis into MSCs. The migration of magnetized MSCs with SPION by static magnets was seen in vitro. The auditory hair cells do not regenerate once damaged, ototoxic mouse model was generated by administration of kanamycin and furosemide. SPION labeled MSC’s were administered through different injection routes in the ototoxic animal model. As result, the intratympanic administration group with magnet had the highest number of cells in the brain followed by the liver, cochlea, and kidney as compared to those in the control groups. The synthesized PCS (poly clustered superparamagnetic iron oxide) nanoparticles, together with MSCs, by magnetic attraction, could synergistically enhance stem cell delivery.

Antihypertensive Effects of P. acidiactici LRCC 5229 (LP5229) and L. casei LRCC 5301 (LP5301)

Ye Jin Lim,Yoon Seok Kim,Wan Heo,Seok Min Yoon,Sung Keum Seo,Mi Ri Park,Si Young Yang,Jun Ho Kim,Jin Hyup Lee,Young Jun Kim 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

A Study on the Condition of the Visually Impaired in Korea

Wan-Seok Lee,Ki-Hun Ye 대한시과학회 2016 대한시과학회지 Vol.18 No.3

목 적: 사회적 소외계층으로 약자인 시각 장애인의 실태파악을 통해 시각 장애인에 대한 관심의 필요성 을 인식시키고, 시각 장애인 연구에 기초자료로 사용하고자 한다. 방 법: 한국보건사회연구원에서 조사한 2011년 장애인실태 자료를 분석하였으며, 조사값은 SPSS 18.0을 사용하여 분석하였고, 유의수준은 p<0.05를 기준으로 하였다. 결 과: 시력장애는 남성은 149,720명(58.3%), 여성은 103,429명(40.3%), 그리고 시야결손장애는 남성 2,444명 (1.0%), 여성은 1,248명(0.5%)으로 시각장애인은 남성이 여성보다 다소 많은 수치를 나타내었다. 발생 시기는 출생 전 또는 출생 시가 8,186명(3.2%), 돌 이전 10,452명(4.1%), 그리고 돌 이후 23,8037명 (92.6%)로 나타났다. 여성의 장애 등록 증가율이 남성보다 2배 높았으며, 시각장애인의 3개월 동안 월 평균 소득은 51.4만원으로 나타났다. 결 론: 시각 장애인의 시력 실명 예방과 시력 회복을 위한 재활 프로그램 등이 정부 차원에서 필요하며, 무엇보다 더불어 함께 사는 사회로 가기 위해서는 장애인의 인권과 권리에 대한 깊은 관심이 필요할 것이다. Purpose: This research is going to show the necessity of a concern and the data as basis for the research of the visual disabled through condition analysis of the visually impaired as second-class citizen. Methods: The date were analyzed through Korea Institute for Health and Social Affairs document 2011. Statistical analyses were performed with SPSS 18.0 and p-value less than 0.05 was considered statistically significant. Results: Visual impairment of male and female was 149,720 people(58.3%) and 103,429 people(40.3%), and visual field defect impairment was male 2,444people(1.0%) and female 1,248people(0.5%). Visual disabled of male was more than female. Time of occurrence showed antenatal or birth 8,186people(3.2%), before the celebration of a baby’s first birthday 10,452 people(4.1%), and after the celebration of a baby’s first birthday 238,037 people(92.6%). Female disabled registration rate increasement showed twice higher than that of male’s. 3 month average earnings of the visually impaired showed 51.4 ten thousand won. Conclusions: There is need for blindness prevention and rehabilitation program for recovery of eyesight for the disabled at government level. In addition, we need to have interest about the human rights of the disabled.

The Huge ovarian mass mimicking a uterine sarcoma

( Ye Jin Seon ),( So Ryeoung Kim ),( Ji Suk Han ),( Hee Young Ahn ),( Eun Jin Heo ),( Yun Dan Kang ),( Jong Soo Kim ),( Jin Wan Park ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Uterine sarcomas are rare mesodermal tumors that account for 3-7% of uterine cancer. They are a heterogeneous group of tumors and have poor prognosis. It is difficult to diagnosis at preoperatively, that the positive predictive value using image study such as Abdominal Pelvis computed tomography (AP-CT) or Pelvis magnetic resonance (MRI) scan is very low. We present a rare case of the primary ovarian fibrothercoma mimicking uterine sarcoma. A 89-years old postmenopausal women present with progressive abdominal pain and palpable abdominal mass. AP-CT revealed about 16cm sized uterine was suspected uterine sarcoma. Tumor markers are normal range. She underwent exploratory laparotomy. There are a huge irregular hard mass with several feeding vessels originated right ovary and grossly normal uterus and left adnexa. A surgical exploration was performed within whole abdomen. there was no metastatic nodules. we performed Right salpingo-oophorectomy and requested frozen biopsy at pathologist. It was a ovarian stromal tumor. Therefore, we performed total hysterectomy, Left salpingo-oophorectomy and both pelvic lymph node biopsy. The final pathologic diagnosis documented an ovarian fibrothecoma. This case reported an unusual case of the primary ovarian fibrothecoma mimicking uterine sarcoma.