Streptococcus salivarius의 요소분해효소 활성에 관한 연구

정상백,최호영,민병순,박상진,이진용,최기운 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

Dental caries is induced by organic acids produced by oral bacteria. In order to prevent dental caries, therefore, it is essential to maintain neutral pH in the oral cavity. Urea plays a major role in oral pH homeostasis. Urea is hydrolyzed by bacterial ureases to ammonia, causing a pH elevation, Streptococcus salivarius has been shown to be a major contribution to oral ureolysis. Synthesis of urease by S. salivarius appears to be constituitive, but can be greatly enhanced by low pH. It is, therefore, conceivable that ureolytic activity of S. salivarius from a carious lesion is greater than that of the bacterium from a healthy tooth. In the present study, urease activity of S. salivarius isolates from dental plaque of carious lesions was compared with that of the isolates from plaques of the teeth and the dorsum of the tongue; 45 S. salivarius strains were isolated from carious lesions(>C2) of 21 individuals with dental caries and 30 strains from 10 individuals without dental caries. The results were as follows: 1. All the 21 individuals with dental caries harbored ureolytic S. salivarius whereas 3 of 13 individuals without dental caries harbored non-ureolytic strains of S. salivarius. 2. All the 45 S. salivarius isolates from carious lesions showed urease activity. In contrast, of 30 isolates from individuals without dental caries, 17 isolates(56.7%) did not demonstrate urease activity, or if any, very little(<5μmol/min/mg). 3. Urease activity of the isolates from carious lesions was greater than that of the isolates from individuals without dental caries:the urease activity ranged from 42 to 381μmol/min/mg and from 0 to 208μmol/min/mg, respectively. 4. At acid pH(5.5), the isolates which showed intermediate urease activity at pH 7.0 demonstrated even higher activity whereas the isolate with no or lower urease activity did not show any significant difference in their activity. However, the isolates with the greatest urease activity from both individuals with and without dental caries, exhibited a rather much lower urease activity at pH 5.5. The overall results suggest that isolates may have their own urease activity but the isolates exposed to chronic acidic environment of the carious lesion might elevate urease activity of S. salivarius, which in turn, might influence on survival of S. salivarius itself and other bacteria establishing a new oral bacterial ecosystem.

기름골 기름의 산화안정성 및 지방산조성

송진,방진기,박희운,박충범,성낙술 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

착유방법을 달리하여 얻은 기름골 기름을 37℃와 60℃에서 저장 중 산화안정성을 조사한 결과는 다음과 같다. 1. 저장 중 기름의 산가는 기간이 경과함에 따라 증가하였는데, 37℃에서 저장한 것보다 60℃에서 저장한 것이 높게 나타났고, 유기용매로 추출한 기름의 산가가 가장 높았다. 2. 저장 중 기름의 과산화물가는 37℃에서 저장한 기름의 경우 점차적인 증가를 보이다 저장 120일 이후 급격한 증가를 보이고 150일 이후 다시 감소하는 경향을 나타내었고, 60℃에서 저장한 기름은 37℃에서 저장한 기름보다 같은 저장기간에서 과산화물가 더 높게 나타났고, 그 경향은 저장 210일째까지 증가가 지속되어졌다. 3. 지방산은 oleic acid가 가장 주된 지방산으로 존재하고 있었고, 지방산은 저장기간이 경과함에 따라 palmitic acid, stearic acid, linolenic acid에서 근소한 증가와 oleic acid, linoleic acid는 약간의 감소 경향을 보였으나 저장에 따른 뚜렷한 변화는 볼 수 없었다. This study was carried out to investigate the oxidative stability and fatty acid composition during storage of chufa oil. Chufa oil was roasted at 170℃, 150℃, non-roasting or solvent extracting and stored at 37℃ or 60℃. The acid value and peroxide value of chufa oil at 60℃ were higher than their at 37℃. The acid value of chufa oil showed the trend of increase in all the samples and that of solvent-extracted oil was the highest during the storage period. The peroxide value of oils was gradually increase on the 120th day but significantly increased between 120th and 150th day. Oleic acid was the major fatty acid in chufa oil. Palmitic acid, stearic acid and linolenic acid increase and oleic acid and linoleic acid decreased during storage period. They were changed slightly and the obvious differents were not observed according to storage period.

필름뱃지의 개인선량평가 알고리즘 개발

정운관,백승진,박재덕 조선대학교 에너지.자원신기술연구소 2000 에너지·자원신기술연구소 논문지 Vol.22 No.2

The purpose of this paper is to develop dose evaluation algorithm for film badge and describe the results of the personnel dosimetry performance test in KINS. Reference radiations irradiate seven categories, except the neutron category prescribed in the ANSI N13.11-1993, and evaluation algorithm have been obtained from dose characteristic curves. The performance criterion in a given category, sum of the absolute value of the bias and the standard deviation value of the performance quotient, were estimated by the use of film badge algorithm and dose equivalents according to the standard method. As a result, the performance index in a given category showed a good agreement less than tolerance level, for the deep and shallow dose equivalents or absorbed dose Development algorithm can be used for dose evaluation of film-badge personnel dosimetry.

개인피폭선량 알고리즘의 평가

정운관,심재학,김영진 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.2

This paper describes experimental evaluation of exposure dose algorithm for film badge and the results of the personnel dosimetry performance test. Reference radiations irradiate seven categories, except the neutron category prescribed in the ANSI N13.11-1993. The performance criterion in a given category, sum of the absolute value of the bias and the standard deviation of the performance quotient, was estimated by the use of film badge algorithm and dose equivalents according to the standard method. As a result, the performance index in a given category showed a good agreement less than tolerance level, for the deep and shallow dose equivalents or absorbed dose, and this study is satisfied with quality decision cnterion of the film badge personnel dosimeter and intercomparison results.

6MV 전자 선형 가속기 광자선의 전자오염

유명진,김동원,정운혁 대한방사선 방어학회 1988 방사선방어학회지 Vol.13 No.1

부산의대 부속병원의 전자선형가속기 (Mevatron 67) 장치를 이용하여 표면선량의 원인이 되는 광자선의 전자오염에 대한 연구를 하였다. 표면선량은 조사야의 크기에 따라 증가하는 경향을 나타내었다. 빔내에 트레이를 설치했을 때와 트레이를 제거했을 때 표면선량의 변화와 전자오염정도을 관찰하였다. 전자오염을 감소시키기 위해서 구리 필터를 트레이 밑에 부착하여 그 효과를 분석하였다. 아울러 거리를 변화시켜가며 선량의 변화를 관찰하여 최대의 피부보호효과를 유지할 수 있는 최적의 SSD와 TSD를 구하였다. 이런 모든 결과들은 방사선 치료환자의 피부보호에 이용될 것이다. The 6 MV photon beam of a linear accelerator (Mevatron 67) was studied for electron contamination. The surface dose, attributable almost entirely to contamination electrons, has a linear dependence on field width for square fields and an inverse square dependence on distance from the bottom of the fixed head assembly. Build-up and surface dose measurements were taken with and without an acrylic blocking tray in place. Further measurements were made with a copper filter designed to reduce secondary electrons emitted by photon interactions with the acrylic tray. The results are discussed in relation to skin sparing effect for radiation therapy patients. To achieve the maximum skin sparing effect, the selection of the optimum SSD and TSD is needed.

3차원 그래픽스를 이용한 실시간 로봇 모니터링 및 예측

김진구,정다운,전재욱 成均館大學校 科學技術硏究所 1997 論文集 Vol.48 No.1

In industrial automation lines, the main method to transfer information,, about robots is using some digital I/O in robot systems. Through these digital I/O, the state of each robot task is monitored whether it has been performed correctly or not. But this method can only communicate some informations among predefined states of each robot task and do no informations about other states during each robot task. In order to monitor detailed states of robot systems during each task, robot systems have used a video display or a graphics system. Some researches have used graphics systems to make real-time display and replay in addition to off-line simulation. This paper proposes a technique to monitor real time robot motions and to predict robot motions by using 3-d graphics. A graphics system is used to represent a virtual robot so that some c ordination between a real robot and this virtual robot can be achieved. With this coordination, monitoring and prediction of robot, motions will be performed.

광케이블 유지보수를 위한 수동 B-NT1 모듈 개발

엄진섭,함청운 江原大學校 産業技術硏究所 1995 産業技術硏究 Vol.15 No.-

The reference model for UNI (User Network Interface) of B-ISDN is consisted of three function groups (B-NTI, B-NT2 and B-TE). In this study, we constructed passive B-NT1 module which can be utilized for optical link management and repair, one of main functions of B-NT1. For the convenience of installation and management, this module is only consisted of passive optical devices. And at a local exchange center linked with the module, the broken zone of optic link is able to be monitored without interrupting services. In addition, we performed the power burget analysis for the design of efficient optic link including this module.

골아세포 MC₃T₃-E₁의 증식과 기능에 Insulin Like Growth Factor Ⅰ, Ⅱ 및 부갑상선 호르몬이 미치는 영향

윤현구,정호연,양인명,김성운,김진우,김광원,김영설,최영길,서광식 대한내과학회 1992 대한내과학회지 Vol.42 No.1

MC₃T₃-E₁골아세포군을 이용하여 IGF-Ⅰ과 Ⅱ를 투여하고 DNA합성과 골아세포기능을 측정하기 위해 PTH에 대한 C-AMP반응정도, alkaline phosphatase 활성도를 측정하여 다음과 같은 결과를 얻었다. 1) IGF-Ⅰ과 Ⅱ는 모두 DNA합성이 어느 농도까지는 증식효과를 나타내지만 그 이후에는 억제되는 양상을 보였다. 2) PTH에 대한 골아세포의 C-AMP반응으로 증가되었지만 IGF-Ⅰ과 Ⅱ의 투여시 농도변화에 따른 의미있는 변화는 없었다. 3) 본 실험의 조건에서 alkaline phosphatase 활성도는 증가되지 않았다. 이상의 결과로 MC₃T₃-E₁ 골아세포에 IGF-Ⅰ과 Ⅱ는 세포의 증식을 자극하여 PTH는 C-AMP생성을 촉진하는 효과가 있었다. The effect of insulin-like growth factor (IGF) Ⅰ and Ⅱ on cloned MC₃T₃-E₁ cells that had osteoblastic activity was examined by [³H] thymidine incorporation into DNA, cyclic AMP responsiveness to parathyroid hormone (PTH), and alkaline phosphatase activity. IGF Ⅰ and Ⅱ stimulated the growth and proliferation of osteoblastic cells at 20ng/㎖ and 50ng/㎖, respectively. We next examined the effect of IGF-Ⅰ and Ⅱ on the expression of osteoblastic activity. Adenylate cyclase response of PTH to IGF-Ⅰ and Ⅱ-treated cells showed maximal stimulation at 10 and 50㎚PTH, respectively, but IGF Ⅰ and Ⅱ induced limited activity of osteoblastic function. In conclusion, IGF-Ⅰ and Ⅱ were potent stimulators to the growth of osteoblastic cells but did not have an immediate effect on expression of osteoblastic function in this experiment.

서울 올림픽에 관한 年齡別 意識構造 調査

金永煥,崔龍雲,鄭眞宇 慶北大學校 師範大學 體育學硏究會 1986 體育學會誌 Vol.14 No.-

With the subject of 870 citizen in Taegu, 29 events, based on the success of '88 Seoul Olympics, were prepared and distributed and analyzed. The result is as follows: 1. The intelleetual level of Olympics is common(56.4) by TV(43.4), effective public media, and newspaper(33.9). 2. The success of Seoul Olympic is optimistic(69.7), Accordingly, national economy will be activated(66.9), and also, politics, diplomacy and security will be effective(33.3). 3. The experted events of medals in seoul Olympics equal in Los Angeles and the mumber of expected medals 10 to 12(26.2). 4. The maneuvering of interference from North Korea. of Seoul Olympics suggests high possibility(80.9) and the participation of communist countries is estimated in half(57.1) 5. The participation of professional performersis pro(39.6) and com(35.9) and the comcenturated training of younger generation is positive Also, the degrea of participation is very high(89.1). 6. The prospect for business of the Ministry of Atheletics will be dull(70.5), and the precedence of national atheletics depends on the institutional guarantee for the opportunity of participation(39.8) and the expension in facilities(30.9).

Treadmill exercise inhibits hippocampal apoptosis through enhancing N-methyl-D-aspartate receptor expression in the MK-801-induced schizophrenic mice

Jin Woo Chung,Jin-Hee Seo,Sang-Bin Baek,Chang-Ju Kim,Tae-Woon Kim 한국운동재활학회 2014 JER Vol.10 No.4