Methanol 자화성 효모에 의한 균체 단백질 생산

권태종,이동희,정호권,최태부 건국대학교 1992 學術誌 Vol.36 No.2

SCP producing yeast JAM -3032 that has high productivity potential with methanol as carbon and energy source was isolated from soil, and conditions for SCP production, nutritional values, and alcohol oxidase of the microorganism were investigated. Isolated strain JAM-3032 was identified as Pichina on the morphological, cultural, physiological and biochemical characteristics. The production of cell mass reached at maximal level after 54 hrs cultivation at 28℃, pH 6.0 in the medium containing methanol 3.0%, yeast extract 0.8%, K2HPO4 0.1%, KH2PO4 0.1%. MgSO4ㆍ7H2O 0.05%, alanine 0.01%, nicotinic acid 0.04%, and biotin 0.04%. Dry cells of Pichia sp. JAM -3032 was composed of proteins 53.3%, carbohydrates 31. 5%, nucleic acids 5.6%, lipids 2.9%, ash 6.4% and amino acid composition of the proteins was satisfied in FAO reference. Biosynthesis of the alcohol oxidase of the strain JAM -3032 was induced by methanol. The enzyme was purified by treatment of ammonium sulfate fractionation, DEAE-Sephadex A-50 ion exchange chromatography and Sepharose 6B gel nitration. The purified enzyme was homogeneous on SDS-PAGE, and molecular weight was estimated to be about 520,000 D that was consisted of 8 subunits containing FAD as coenzyme. The optimal pH and temperature for the enzyme reaction was 7.5 and 37℃, respectively. Activation energy was 17.1Kcal/mol and half inactivating temperature was 40℃. The enzyme activity was inhibited by Ag+, Cu2+ and Hg2+ ions remarkably. The enzyme acted on methanol specifically, and its Km value was 0.89 mM.

Aspergillus sp.(C-58) 菌株가 生産하는 Inulin 分解酵素에 관한 硏究 : 第1報:액체 배양에 따른 효소생산의 배양조건 PartⅠ:Effect of Culture Conditions on the Submerged Cultivation

權泰種 건국대학교 1980 學術誌 Vol.24 No.2

A strain of Aspergillus species (C-58), isolated from 52 kinds of soil sample, was found to produce a strong inulase in the culture filtrate. Selected strain produced extracellular inulase when cultured in a medium containing not only glucose but also pentose, hexose and inulin, respectively. And obtained results were as in the following. 1.Selected strain was identified to Aspergillus species. 2.Culture filtrate is responsible for the hydrolysis not only of the simple β-fructoside such as sucrose but also of the polysaccharide, inulin. 3.The most effective starting concentration of glucose was 1.5% and reasonable period of shaking culture was 8 days in the preculturing. 4.The enzyme production was found to increase significantly with the addition of corn steep liquor (1.0%), NH4H2PO4 and (NH4)2PHO4 for the nitrogen sources to the medium. 5.The enzyme production was increased with the addition of NaCl, KCI and MgSO4 7H2O for the salts. 6.Optimum initial pH for the production of inulase by shaking culture was 5.0. 7.The addition concentration of CaCO3 was 1.0% after 24 hrs cultivation in the cultures.

Aspergillus sp.(C-58) 菌株가 生産하는 Inulin 分解酵素에 關한 硏究 : Part2. The effect of culture conditions on the soild cultivation 第二報 固體培養에 따른 培養條件

權泰鍾 건국대학교 1981 學術誌 Vol.25 No.1

A strain of Aspergillus species C-58 was produced much inulin hydrolyzing enzyme(EC 3.2.1.7) in wheat bran solid medium than glucose liquid medium Optimal conditions for the inulase production by Asp. sp. C-58 strain on wheat bran solid medium were established. The results were as follows. 1.Optimal content of moisture on the inulase production at the wheat bran solid medium was about 50% 2.Inulase production on the wheat bran medium from Asp. sp. C-58 strain was increased by addition of 3%-glucose solution. 3.Organic nitrogen sources, inorganic nitrogen sources and inorganic salts which there were no or very little increase in wheat bran medium. 4.Effectual initial pH on the inulase production were pH 4.0 to 5.0. 5.Optimum culture period on the inulase production was to be 5 days.

스테인리스강 용접부의 잔류응력이 피로특성에 미치는 영향

권종완,양현태 한국공작기계학회 2001 한국생산제조학회지 Vol.10 No.5

In the weldments, the crack propagation rate is changed due to the residual stress. The crack propagation rate is high in the region with the residual stress. However it shows the same behavior with the base metal in the region that does not include the residual stress. The fatigue crack growth rate for the material with residual stresses can be predicted more precisely by using the effective stress ratio. The difference between experimental results and prediction results seems to be due to the redistribution of the residual stresses and microstructural change.

α-Amylase Inhibitor 생산균 Streptomyces sp. AI-118의 분리 및 동정

권태종,서동진 建國大學校 附設 産業技術硏究所 1992 논문집 Vol.17 No.-

a-amylase의 효소작용을 저해하는 물질을 생산하는 균주를 토양에서 분리, 동정하였으며 저해물질 생산의 최적 조건을 검토하였다. 본균을 방선균 종정법에 따라 결과 새로운 균주로 판명되어 Streptomyces sp. AI-118이라고 명명하였다. 본 균주는 탄소원으로 corn starch 3.0%, 질소원으로 soy bean meal 1.0%, 무기염으로 NaCI 0.05%, MnSO₄·4H₂O 0.0001%, CuSO₄·5H₂O 0.0001%를 사용한 배지에서 initial pH 6.0, 30℃ 그리고 배양 72시간일 때 최대의 저해물질을 생산하였다. A microorganism, strain AI-118, capable of producing a new a-amylase inhibitor in culture broth was isolated from soil and identified as Streptomyces sp. on the morphological, cultural and physiological characteristics. The preferable medium composition has been determined to be as follows:3.0% corn starch, 1.0% soy bean meal, 0.05% NaCl, 0.05% K₂HPO₄, 0.0001%MnSO₄· 4H₂O and 0.0001% CuSO₄·5H₂O(pH6.0). The inhibitor was maximally produced after 60-80hr cultivation in a jar fermentor at 30℃.

Photoshop을 이용한 하악 후퇴 수술후의 연조직 안모 예측

권대근,김종배 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.4

Various computer software has been applied to predict soft tissue profile after orthognathic surgery. In this study, the Photoshop program was introduced to simulate the surgical result. The purpose of the study was to evaluate reliability of the program in prediction of profile changes as a result of mandibular set-back surgery. Based on predicted hard tissue changes, soft tissue changes were recorded with pre- and post-operative lateral cephalogram of 26 patients with skeletal Class Ⅲ deformity. All patients underwent bilateral sagittal split osteotomy. The mean mandibular set-back was 6.2㎜ (Pog) and soft tissue moved 6.3㎜, 100% in Pog' point post-operatively. Difference between the between real horizontal soft tissue changes and predicted changes has not been detected statistically. However, the result revealed vertical soft tissue profile prediction was unsatisfactory. This prediction method can be used as additional informative tool for the patient explanation.

갑상선기능항진증과 원발성항인지질항체증후군에 동반된 무도병 환자 1례

박종원,김동현,박태환,권오상 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

대장암 환자에서의 담즙 암태아성 항원(CEA)

김종우,박용태,정철운,권성원,장항석,손석우,안대호,이경포 대한대장항문학회 2000 Annals of Coloproctolgy Vol.16 No.6

Naringinase 생산균의 분리 및 효소의 생산과 정제

권태종 건국대학교 산업기술연구원 1999 건국기술연구논문지 Vol.24 No.-

미숙한 감귤과 포도에 존재하며 쓴맛을 띠는 naringin을 분해하는 naringinase를 강력하게 생산하는 미생물을 분리하기 위하여 76점의 토양과 25점의 부패한 과일로부터 총 582 균주의 사상균을 분리하였다. 분리한 균주들 가운데 가장 naringinase 생산력이 우수한 YK-517을 배양한 결과 및 형태학적 성질로부터 Aspergillus niger YK-517로 동정하였다. 공시균주로부터 naringinase 생산성을 조사한 결과, 탄소원으로 1% naringin과 질소원으로는 0.5% yeast extract였고 인산염으로는 0.1% K₂HPO₄가 가장 높았다. 또한 금속염으로는 0.1% CaCO₃였으며 초발 pH는 5.0으로 하여 30℃에서 72시간 배양하였을 때 naringinase 생산이 가장 높았다. Naringinase는 Ultrafiltration과 Hydroxyapatite column chromatography 및 Sephadex G-150 column chromatography에 의하여 정제되었다. 정제된 Naringinase는 그 분자량이 약 60,000이였고 2개의 동일 subunit로 구성되었다. In order to produce naringinase which hydrolyzes bitter naringin in underripe citrus and grape, fungal strains were isolated from soil and rotted fruits. The strain YK-517 which showed the highest activity among 582 fungi was identified as Aspergillus niger on the cultured and morphological characteristics. A. niger YK-517 strain produced the enzyme maximally when grown at pH 5.0 and 3 0℃ for 72 hours in the medium consisting of 1% naringin, 0.5% yeast extract, 0.1% K₂HPO₄ and 0.1% CaCO₃. The enzyme was purified by ultrafiltration, hydroxyapatite column chromatography and Sephadex G-150 gel filtration. Molecular weight of the purified enzyme was estimated at about 60,000 dalton composed of 2 identical subunit.

역행적 골수정 삽입술을 이용한 대퇴골 과상부 골절의 치료

권진우,신승호,조원호,성기호,김기환,손경태,이상훈,박세원,배종문,권태우 대한골절학회 2003 대한골절학회지 Vol.16 No.4