數種 漢藥材가 肝癌細胞(Hep G2)와 마우스 腹腔 大食細胞에 미치는 影響

康城溶,韓宗鉉,趙南根 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

The action mechanism of herb drugs on the cytotoxicity and apoptosis in tumors undoubtedly are diverse. Apoptosis often is particularly prominent near foci of confluent necrosis, where mild ischemia is likely to be involved in its initiation. Apoptosis in tumors found in living body is occured by cytokines released from macrophage or cytotoxic T-lymphocyte, but the mechanism is not known accurately. A variety of cancer chemotherapeutic agents have been shown to induce extensive apoptosis in rapidely proliferating normal cell population, lymphoid tissues and tumors. The way in which anti-cancer drugs induce apoptosis is unknown. However, there is an additional important consequence of the realization that anti-cancer drugs mediate their therapeutic affects by triggering apoptosis. The activated macrophage can selectively destroy tumor cells Therefore, macrophage-mediated tumor cytotoxicity can have a important significance. Recently, it has been reported that some crude drugs stimulates IFN-v production in T-lymphocyte, and IFN-v stimulate nitric oxide production in peritoneal macrophage. The nitric oxide can affect the cytotoxicity and apoptosis in tumor cells. In this study, antineoplastic activity against human hepatocellular carcinoma cell line(Hep G2) was tested in four species of herb drugs. The plant materials were extracted with water, and the cytotoxic activity was tested using a calorimetric tetrazolium assay(MTT assay), the apoptosis was tested using a DNA electrophoresis and flow cytometry. The nitric oxide production from mouse peritoneal macrophage was tested using a Griess method. The four species of herb drugs were Arisaemitis Tuber, Bupleuri Radix and Coptidis Rhizoma extracts against the proliferation of Hep G2 cells showed cytotoxicity at the concentration of less than 100ug/㎖, and Bupleuri Radix and Coptidis Rhizoma extracts increased the cytotoxicity of mitomycin C and Coptidis Rhizoma extracts increased the cytotoxicity of cisplatin on Hep G2 cells. Bupleuri Radix and Coptidis Rhizoma extracts against the proliperation of BALB/c 3T3 cells showed cytotoxicity, and Coptidis Rhizoma extracts aginist the proliperation of mouse thymocytes showed cytotoxicity at the concentration of less than 100ug/㎖. Arisaemitis Tuber extracts increased nitric oxide production from macrophage.

2007년 대한감염학회 권장 성인 예방접종 권장안

강진한,김홍빈,손장욱,이상오,정문현,정희진,최영화,최정현,최준용,최희정 대한감염학회 2008 감염과 화학요법 Vol.40 No.1

In 2007, the Korean Society of Infectious Diseases recommended an immunization schedule for adults residing in Korea. This recommendation includes four categories: standard immunization for healthy persons, supplementary immunization for persons with underlying illnesses, recommendations for health-care personnel and for international travelers. The Standard Immunization Schedule consists of universal vaccination (tetanus-diphtheria/tetanus-diphtheria-pertussis (Td/Tdap); hepatitis A vaccine for persons aged 20s; human papilloma virus vaccine for women under 26 years old; meningococcal vaccine for military recruits and college students in dormitories; influenza vaccination for persons aged 50 years or more and pregnant women; and pneumococcal vaccination for persons aged 65 years or more) and catch-up vaccination (hepatitis A vaccine for persons aged 30s; hepatitis B vaccine, varicella and MMR vaccines for persons who have uncertain specific immunities). The Supplementary Immunization Schedule recommends 4 vaccines, i.e., pneumococcal, influenza, meningococcal, and Haemophilus influenzae type b, appropriate to the underlying illnesses, and confirmation of the presence of antibodies to hepatitis A and hepatitis B. The Recommendation for Health-Care Personnel includes universal vaccination (influenza and pertussis) and catch-up vaccination (hepatitis B, measles-mumps-rubella and varicella for seronegative health-care personnel). The Recommended Immunization for International Travelers focuses reinforcement of vaccinations for healthy persons and includes certain immunizations necessary for international travel (yellow fever, meningococcal, plaque, tick-borne encephalitis, and cholera). These guidelines can be seen at www.ksid.or.kr.

삼각형 모양에 의한 회절현상에 관한 연구

강성수,성덕용,육도진,홍성대,이원진 대구산업정보대학 2003 논문집 Vol.17 No.-

A review of diffraction phenomena for double slit and regularly triangular aperture has been done. First, as a width b and a space d of double slit vary, a diffraction pattern has been calculated by theory. Second, an intensity of diffraction light and separation between fringes for slit has been measured. And, we can expect that the calculated diffraction pattern for a regularly triangular aperture has three symmetric axes.

陳皮의 腦血流力學에 관한 硏究

康城溶 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

Oriental medicine prescription has been used for the treatment of various clinical symptoms associated with cerebral However, single herb drug does not used cerebral apoplexy and there mechanisms of sction have not been apoplexy. defined, and it is not yet known what effects they have on the hemodynamics of cerebral circulation. The study was aimed to investigate the effect Citri Pericarpium (CP) on the vascular systems including changes The changes in rCBF in blood pressure (BP), and regional cerebral blood flow (rCBF) of male Sprague-Dawely rats. were determinated by laser-Doppler flowmetry 1. Blood pressure was increased by CP in a dose-dependent manner. 2. rCBF was increased by CP in a dose-dependent manner. 3.Pretreatment with propranolol(3mgjkg), methylene blue( Imgjkg), ODQ( 10 ji gjkg) and L-NNA( Imgjkg) was inhibited by CP in a dose-dependent manner in rCBF. 4. Pretreatment with propranolol(3mgjkg), methylene blue( Imgjkg), ODQ( 10 ji gjkg) and L-NNA( Imgjkg) was inhibited These results suggest that CP causes a diverse response of blood pressure and regional cerebral blood flow(rCBF). The increased in rCBF is also mediated by adrenegic 13 recepter, nitric oxide synthzase and guanylate cyclase.

생쥐 2-세포기 수정란의 초급속동결

강만종,이철상,한용만,유대열,이경광 제주대학교 농과대학 제주도축산문제연구소 1991 畜産論叢 Vol.6 No.1

This study was carried out in order to investigate eggects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos Mouse 2-cell embryos. fol-lowing dehydration by exposure to DMSO and sucrose. were directly immersed into liquid nitrogen and thawed in 37℃ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium a t various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO conentrations (82.6%). However, When sucrose concentrations of 0.25 and 0.5M were added to the freezing medium with 3.0M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time(2.5min) in 3.0M DMSO +0.25M sucrose(85.9%). 3. The development rate of embryos at in vitro 2-celL in vivo 2-celL solution control and untreated control was 84.6%, 90.9%, 89.9%. and 89.7%. respectively.

成長과 變化에 대응하는 캠퍼스 計劃에 관한 기초적 硏究

강성택,조용수 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

This study aims at providing a fundamental guide for the growth of university and extension of its facilities. Growth types of campuses were studied and analysed by theoretical and empirical research on six sample universities. Finally, some planning guides for organic growth and spatial composition of campus facilities were proposed for the design of campus masterplan.

오존공정을 이용한 염색폐수의 처리특성에 관한 연구

강용태,원태준,한상윤,고영발,박경덕 東亞大學校 建設技術硏究所 2008 硏究論文集 Vol.32 No.1

The wastewater from textile industry typically containing high alkalify, salts, organic matter, and high color. Textile wastewater is usually treated by anaerobic-aerobic activated-sludge process. The activated-sludge process is effective the reduction of organic matter, but generally does not remove color. Ozone process is one of the most effective process in removal of color for the textile wastewater. Ozone has a strong oxidized dissolution of organism, deceleration, deodorization, fumigation, etc., regarding to water. This study focused on the color removal of textile wastewater. In this study, textile wastewater was treated strongly with ozone at 650mg/hr and the flow rate through ozone contact tank was adjusted to 5L/min. In an application Ozone treatment, Color was generally decreased with time, however, COD removal effect was not that good. In this study, the removal efficiencies of CODcr and Color were 23.5%, 80.4% respectively.

다수의 냉동시스템에 의한 저온 저장고의 에너지 성능평가

강동언,김성만,운종호,이의준,박영하,현명택 濟州大學校 産業技術硏究所 2000 산업기술연구소논문집 Vol.11 No.1

The cool storage system(CSS) is a specific building that is required only cooling load without heating during the whole year. CSS uses a electric energy which is very expensive and is required much amount of energy due to temperature differences between interior and ambient temperatures. The purpose of this study is to assess the energy performance of the multi-unit cooler operating control system. For the prediction of its performance, two types of test (KIER). First cell is equiped with one .are installed a t Korea Institute of Energy Research large unit cooler. and second cell consists of two small unit coolers. According to the results of experiments using these cells. the multi-unit cooler operating control system is found to be effective in energy saving and beneficial for storage due to much less deviation of temperature.

Biosurfactant 생산 효모 Rhodotorula sp. G-1의 분리 및 Biosurfactant 생산

강상모,이철수,김영찬 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

미생물 기원의 계면활성제(microbial-surfactant, biosurfactant)를 생산하는 균주를 찾기위해 여러 지역의 토양에서 생산균주를 분리, 동정하였고 이 균주의 발효생산 최적 조건을 검토하였다. 서울과 근교의 주유소 주변 토양에서 20여 균주를 분리하였으며 이들 중 배양액의 표면장력을 가장 많이 저하시켜 다른 균주에 비하여 상대적으로 활성이 좋은 G-1 균주를 분리하였다. 이 균주의 형태학적 및 배양학적인 특성에 의해 동정한 결과 아직까지 biosurfactant 생산균이라고 알려진 바가 없는 새로운 효모인 Rhodotorula sp. G-1로 동정되었다. 이 균주의 최적 biosurfactant 생산 조건은 탄소원인 soybean oil 4%, glucose 2%, yeast extract 0.5%, 인산염농도 0.1%, pH 6.0, 30℃에서 3일간 진탕배양했을 때 surface tension 활성이 가장 높았다. 그리고 처음에는 탄소원으로 soybean oil만을 첨가하여 배양하다가 glucose를 90시간만에 공급한 경우 처음부터 soybean oil과 glucose를 동시 첨가한 것에 비하여 surface tension 활성이 14% 증가함을 알 수 있었다. 이렇게 하여 biosurfactant 양은 15.0 g/l 농도로 얻을 수 있었다. Some microorganisms including yeasts produce surface tension-decreasing biosurfactants. The strain G-1, the best producer of biosurfactant, was isolated from the soil and identified as Rhodotorula sp., which was not discribed any report. The Rhodotorula sp. G-1 produced biosurfactant from vegetable oils, but failed to produce it from n-alkane or carbohydrate. Yeast extract was found to be more effective for the biosurfactant production as nitrogen source than any other inorganic nitrogen source. The composion of the optimal medium contained the following conponents: soybean oil 4%, glucose 2%, yeast extract 0.5%, KH_2PO_4 0.1%, K_2HPO_4 0.1%, MgSO_4 5%, CaCl_2 0.01% NaCl 0.01%, pH 6.o. The surface tension activity was increased to 14% when, at first, the culture broth was fermented with only soybean oil as carbon sourse, and after 90 hours, feeded glucose, than that of glucose and soybean oil added to it simultaneously. The maxium yield of the biosurfactant was about 15 g/l by, after 90 hours, the feeding method of glucose.

헬리코박터 파이로리 균 진단용 ^13C-요소 캅셀의 개발

용철순,김용일,김지만,강성훈,권기철,이종달,김종국,사홍기,최한곤 영남대학교 약품개발연구소 2002 영남대학교 약품개발연구소 연구업적집 Vol.11 No.-

The purpose of this study was to develop a new ^13C-urea-containing capsule for diagnosis of H.pylori. The urea-containing capsules were prepared with various diluents such as polyethylene glycol (PEG), microcrystaline cellulose, sodium lauryl sulfate and citric acid. The dissolution test, ^13C-urea breath test and stability test were then performed on the capsules. Microcrystalline cellulose and sodium lauryl sulfate retarded the initial dissolution rates of urea. However, PEG increased the initial dissolution rates of urea. Furthermore, two formulae composed of PEG,[^13C-urea/PEG (38/1.9 mg/cap)] and [^13C-urea/PEG (38/1.9/1.9 mg/cap)] had the maximum DOB value. about 16 at 20 min, while the formula composed of only 38 mg ^13C-urea had the maximum DOB value at 30 min. The results indicated that PEG improved the sensitivity of ^13C-urea in the human volunteers. The capsule [^13C-urea/PEG (38/1.9 mg/cap)] was stable for at least six months in 25 and 37℃. Thus, a PEG-containing capsule, [^13C-urea/PEG (38/1.9 mg/cap)] would be a more economical, sensitive and stable perparation for diagnosis of H. pylori.