Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Ehlers - Danlos 증후군

주용진,최성문,최영자,성호석 ( Yoing Jin Joo,Sung Moon Choi,Young Ja Choi,Ho Suk Sung ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Ehlers-Danlos syndrome Yoing Jin Joo, M.D., Sung Moon Choi, M.D., Young Ja Choi*, M.D., Ho Suk Sung, M.D. Department of Dermatology, Clooego of Medicine, Inje University, Pusan, Korea We presented a case of Ehlers-Danlos syndrome in a 25-year old female, who had hyperextensibility of skin, skin fragility, cigarette paper-like atrophic scar, joint hyperextensibility and skeletal abnormality of scoliosis since birth. There was no family history. Microscopic and electron microscopic examinations of the skin biopsy specimens from normal appearing skin on the forearm and the site of subcutaneous nodule was compatible with finding of Ehlers-Danlos syndrome. Although the manifestations of this case including marked extensibility of skin, joint, skin fraility and atrophic scar might be in accord with type Ⅰ(gravis) Ehlers-Danlos syndrome, skeletal abnormality of scoliosis did not correspond to this type, but to type Ⅳ(hydroxylysine deficient) Ehlers-Danlos syndrome. We consider this case as a type Ⅳ Ehlers-Danlos syndrome.

五加皮의 效能에 대한 硏究

최민호,이계복,조규원,박진영,김경수,송정석,한종현 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

The purpose of this study is to measure the changes of regional cerebral blood flow (rCBF) and blood pressure (BP) of Cortex Acanthopanacis in rats, and to determine the effect of Cortex Acanthopanacis on phenylephrine (PE) induced contraction of isolated rat thoracic aorts. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure transducer (Grass, USA) in anesthetized adult Sprague-Dawley rats through the data acquisition system composed of MacLab and Macintosh computer. Contractile force was measured with force displacement transducer under 1.5 g loading tension. The result of this experiment was as following; 1. Cortex Acanthopanacis did not effect the changes of rCBF and blood pressure significantly. 2. Contractions evoked by phenylephrine were decreased significantly by Cortex Acanthopanacis 3. L-NNA, ODQ, atropine and indomethacin significantly altered the relaxation of Cortex Acanthopanacis. 4. Propranolol did not change the relaxation of Cortex Acanthopanacis. These results indicate that Cortex Acanthopanacis did not change the rCBF and BP, but Cortex Acanthopanacis can relax PE induced contraction of isolated rat thoracic aorta and that this increasing contraction related to endothelium and various mechanism.

충남대학교병원 마취발전의 경향에 관한 분석 : 1967년부터 - 1996년까지 from 1967 to 1996

최세진,김상수,정규돈,윤석화,신용섭,손수창,이원형,김혜자,이정은 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

To evaluate the specificity and historical trends of the anesthesia in Chungnam National University Hospital, anesthetic experiences of 83,572 in total performed at Chungnam National University Hospital from January 1967 to December 1996 were analyzed statistically and clinically according to age, sex, surgical department, physical status, elective and emergency surgery, premedicants, IV anesthetics, anesthetic methods, inhalational anesthetics, muscle relaxants. The results were as follows : 1. The number of cases has been steadily increased year by year. 2. The ratio of male to female was 52.8% : 47.2%. 3. According to the age, There were 44,905 cases (54%) in the group of 13-45 years. 4. According to the ASA classification of physical status, most of the cases were belonged to the class 1 & 2 (72.5%). 5. The ratio of elective to emergency was 79.4% to 20.6%. 6. Recently, glycopyrrolate has been used increasingly. 7. Thiopental sodium has been mainly used for intravenous induction agent. 8. General anesthesia has been used mainly. 9. Enflurane is the most common inhalational anesthetic agent. but Isoflurane has been used increasingly. 10. Most of muscle relaxants are Pancuronium & Vecuronium(84.5%).

忠州市 主幹線 道路邊 建築外部色彩에 關한 硏究

崔生吉,孫泰鎭,柳顯紀,辛同寅,梁富弘,尹勝照,朴碩鉉 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.2

In order to understand the influence of colours on the urban design of a city, a portion of the central Chung-Ju was chosen as the study area, and three cases were researched as colours. These surveyed colours are analysed in their hue, value and chroma. It was necessary to determine the colours of 272 buildings exterior in all in order formulate the findings presented in this study. The result of this study can be briefly summerised as the following ; 1) The trends in the use of colour turn out like this ; 55.3% of the building were Y(yellow), YR(yellow-green), R(red) Family in Hue, abour 33.9% of them were within 8.0-8.9 and 26.6% were within 7.0-7.9 on the value scale, and about 48.5% of them were within 1.0-1.9, 2.0-2.9 and 3.0-3.9 on the chroma scale. 1) For case 1 on the exterior colours of buildings, about 57.8% were Y and YR family in Hue, about 53.7% were 8.0-8.9 and 7.0-7.9 in Value, and about 49.6% of them were within 1.0-3.9 in chroma. 2) For case 2 on the exterior colours of buildings, about 52.4% were Y and R family in Hue, about 65.1% were 7.0-8.9 in Value, and about 46% were 1.0-2.9 in Chroma. 3) For case 3 on the exterior colours of buildings, about 55.7% were Y and YR Family in Hue, about 72.1% were 7.0-8.9 in Value, and about 31.1% were 2.0-2.9 in Chroma. In summary, the colours of the building surveyed in central Chung-Ju can be characerized as Y, YR, R, N family in Hue, 7.0-7.9, 8.0-8.9 in Value registered in 1.0-1.9, 2.0-2.9, 3.0-3.9 in Chroma. The observed exterior colours show a tendency toward Yellow in Hue, High in Value and low in Chroma. Generally, the majority of the colours is light rather than strong in tone.

전산화단층사진을 이용한 하악골 비대칭 환자의 저작근 평가

최순철,이선복,이진구,이원진,허민석,이삼선 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : To compare the size of the masseter and lateral pterygoid muscle between the affected and the unaffected side of the patients who have the chief complaints of the mandibular asymmetry. Materials and Methods : Twenty two patients (male: 4, female: 18, average age: 21.3 year-old) were radiographed using posterior-anterior (P-A) cephalography and computed tomography (CT). On P-A cephalography, the degree of deviation was determined by the distance from the mentum to the vertical reference line through the crista galli and the anterior nasal spine. On the scanned tracing papers of the maximum cross-sectional area of the masseter and lateral pterygoid muscle using axial CT images, the pixel number was measured. The ratio of the affected : unaffected sides were obtained. For the masseter and lateral pterygoid muscle, the relationship between the muscular volume and degree of skeletal hypoplasia was studied. Results : The half cases showed no skeletal asymmetry. The lateral pterygoid muscle of the affected side was larger significantly than unaffected side (p<0.05). However, there was no significant difference between two sides in the cases of skeletal asymmetry. There was only significant difference in the cases without skeletal asymmetry (p<0.05). Conclusions : To some extent, the slight mandibular hypoplasia could affect the growth of some masticatory muscles.

서울 新村地域의 商街硏究

崔錫珍 동국지리학회 1986 東國地理 Vol.- No.7

Shopping street of an urban community are partly responsible for the economy of the community, and represent the regional characteristics of the urban community. The present study is aimed at examining and explaining the distribution and spatial location of shops and stores located in Shinchon area and their functions. Since there are so many universities located in Shinchon, one of sub-centers of Seoul, university students related functions were focused when examining the functions of the shopping streets. The first consideration, when deciding the regional limit for surveying, is given to the land value distribution(fig. 1). And the shopping streets are divided into sight route for convenience of the analysis. The street 1, the main street of Shinchon area, is characteristic in representing every kind of functions. The street 2, a road leaing to Ewha woman's University, shows a predominance in the business of dress, sundries, foreign goods and leatherware, representing an overall characteristics feature of shinchon shopping area. The street 4, a street leading to Yonsei University, and the street 5, an entry road to Sogang University, are distinctively lined with shops and stores providing entertainment and various kinds of services for university students. And the other street are characterized by various kinds of restaurants and every kind of service shops. In terms of function, the majority of business includes on the whole various kinds of restaurant and daily goods. To be distinctive enough, furniture stores are flourishing along the street 1. In spite of the cultural function as university town, there are rare book stores. In terms of vertical function of the street, among the total 1472 stores surveyed, 939 stores (accounting for 63.8%) are located on the first floor, 116 stores (7.9%) on the second floor, 350 stores (23.8%) on the third floor or higher and 67 stores (4.6%) on the basement, these numerical data show that the vertical development of the store buildings are not satisfactory enough. On the first floor, sundaries stores are heavily distribued, and on the second floor or higher, restaurant, clinics, and entertainment shops are chiefly located. This vertical tendency of function is the similar as that of the downtown area of Seoul. To conclude, shopping street of Shinchon area are characterized by flourishing shops and stores of dress, sundries, foreign goods and restaurant among the streets 2 and 4. How-ever, specialization of business by street is not still differentiated on the whole. This problem must be preferentially taken into account when setting up plans for the redevelopment of this area.

스포츠센터 직원들의 인구학적 특성에 따른 직무스트레스 및 직무만족의 차이

최종삼,조용철,장순용,정석진 龍仁大學校 體育科學硏究所 2002 體育科學硏究論叢 Vol.12 No.1

This study purposes the influence of job stress upon job satisfaction of Sports Centers Employees demonstratively. I set up the employees of sports centers in Seoul and Gyeonggi Province as Population and took 300 employees in 13 sports centers using a stratified cluster random sampling. The actually used data were 278 workers except 22 employees who were judged to respond unfaithfully or to leave out some of the surveyed questions. I made a conclusion as follows through statistical analyses including reliability analysis, frequency analysis, t-test, one-way ANOVA and Scheffe's multiple range test. First, there was a statistical significance according to age, education, position, and working years in the job stress under personal characteristics and personalities of employees in sports centers. Second, there was a statistical significance according to age, education, position, and working years in the job satisfaction under personal characteristics and personalities of employees in sports centers.

Thermus sp. X-1로부터 耐熱性 蛋白質 分解酵素의 最適 生産 條件에 關한 硏究

최정진,권석태 성균관대학교 생명공학연구소 2000 生命工學硏究 Vol.6 No.1

Thermus sp. X-1 was selected as source of thermostable alkaline protease from a survey of genus Thermus. T. sp. X-1 (TX1) protease was found to be inducible. T. sp. X-1 was tested for production of protease by addition of bactotrypton, casein, gelatin, polypepton and sodium glutamate. Gelatin and sodium glutamate were found to be effective for the protease induction. The optimal induction medium for production of protease was composed of 0.6% gelatin, 0.2% sodium glutamate, 0.2% yeast extract and 1X Castenholz salts. The activity of the enzyme in the optimal induction medium increased approx. 2.8-fold compared to the standard medium.

코로나 플라즈마 처리에 의한 HDPE 표면의 개질에 관한 연구

최진숙,전종탁,정봉규,이근대,문명준,이종무 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

We are able to generate the stable plasma in open air and nitrogen using a corona plasma treatment system(CPTS) with a high voltage. This was applied to treat the surface of rather non-polar HDPE(High Density Polyethylene) intended for the improvement of adhesion of over-coated layers such as coatings, printing inks. The surface tension of HDPE was significantly improved by the CPTS. The characteristics of surface layer were evaluated by means of contact angle measurement, ATR-FT/IR spectroscopy, and it was found that functional groups were introduced into the surface layer of the HDPE. The use of nitrogen was increased the surface tension of the HDPE from 33.8 mJ/m^2 to 66.56 mJ/m^2, and highly effective method of HDPE surface modification could be realized.