항산화제 섭취가 탈진적 운동 시 장딴지근의 GPx 및 GR의 활성에 미치는 영향

정성태,김연수,이왕록,박익렬,강현주 서울대학교 체육연구소 2001 서울大學校 體育硏究所論集 Vol.22 No.1

The purpose of this present study was to investigate the effect of maximal exercise and a long-term antioxidants supplementation on antioxidant enzyme(GPx and GR) activity of gastrocnemius muscle in rats. Twenty four male Sprague-Dawley rats(4 weeks old) were used as subjects. The subjects were divided into 4 groups such as, CON(n=6 in control group), NAE(n=6 in non antioxidants exercise group), AC(n=6 in antioxidants control group) or AE(n=6 in antioxidants exercise group). Non-antioxidants group(CON & NAE) fed general food(Samyang, Inc., Korea) for 16 weeks. Antioxidants group(AC & AE) fed the general food and the mixed antioxidants which were consist of vitamin E(200 lu/kg/day), vitamin C(50 mg/rat/day), vitamin B6(250 ug/kg/day), β carotene(300 mg/kg/day) and selenomethionine(0.1 mg/mg/day), The weight of each subject group was weighed 2-3 times per week. After the diet experiment for 16 weeks, exercise groups(NAE & AE) completed acute treadmill running(speed increased gradually to 25 m/min, 15% uphill grade, ∼50±10 min) until exhaustion. Immediately after exhaustive treadmill exercise, all the subjects were sacrificed for the isolation of gastrocnemius. Then, GPx(Glutathione Peroxidase) and GR(Glutathione Reductase) of the gastrocnemlus muscle were assayed. The protein concentration in the muscle and heart was determined by the Lowry method. After finishing the analysis of antioxidant activity, statistical analysis was carried out by two-way ANOVA and independant t-test. The results of this study can be summarized as follows: Maximal exercise increased the GPx and GR activity of gastrocnemius muscle, Further, a long-term antioxidants supplementation more significantly increased the GPx and GR activity of gastrocnemius muscle than those of non-antioxidants group. In conclusion, the present results clearly show that the exhaustive maximal exercise gave rise to increase oxidative stress which led to oxidative damage so that the antioxidant enzyme activity of gastrocnemius muscle were increased. Further, it has been found that the long-term antioxidants supplementation could decrease oxidative stress in the tissues so that the antioxidant enzyme activity of skeletal muscles was more increased.

뇨(尿)중 3-Methylhistidine 함량의 측정과 이용

정수현,서형주,김윤숙,이효구,강덕호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.2

기존의 방법을 일부 수정하여 뇨중 3-methylhistidine을 분석하였다. 뇨중 3-methylhistidine을 fluorescamine 유도체화하여 HPLC에 주입하고 C_18 column과 10 mM acetonitrile/sodium phosphate buffer(pH 7.5)로 분리·용출시켜 형광검출기로 측정하였다. 3-methylhistidine의 체류시간은 7분 이내이었으며, histidine과의 분리상태도 서로 간섭함이 없이 양호하였다. 뇨에 3-methylhistidine을 첨가하고 이를 분석하였을 때의 회수율은 93∼106%로 높은 수준이었다. 체육학과 남학생중 웨이트 트레이닝 단련자와 비단련자를 대상으로 조사한 단기간의 웨이트 트레이닝에 따른 뇨중 3-methylhistidine 함량의 변화는 두 집단 모두 웨이트 트레이닝후의 3-methylhistidine 분비량이 유의하게 증가하였다. A modified method is given for the precolumn derivatization and subsequent high-pressure liquid chromatographic seperation of 3-methylhistidine from urine. The elution contained isocratic solution with acetonirile and 10 mM sodium phosphate(pH 7.5) requires less than 7 min. The recoveries of 3-methylhistidine from urine control were 93% to 106%. 3-Methylhistidine determinations were performed on urine samples from volunteers who were both male trained and non-trained physical undergraduates. As the result, urinary 3-methylhistidine content of volunteers increased significantly after weight training.

프롤린 함량 증가 및 내건성 강화 관련 새로운 RING-Type E3 유비퀴틴 리가아제 애기장대 atrzf1 돌연변이체의 생리적 기능 연구

주현우,민지희,정문수,김철수 전남대학교 농업과학기술연구소 2021 농업생명과학연구동향 Vol.59 No.-

The covalent attachment of ubiquitin to proteins plays a fundamental role in the regulation of cellular function through biological events involving abiotic of biotic stress responses, immune responses, and apoptosis, Here, we characterize the biological function of the Arabidopsis thaliana RING Zinc Finger 1 (AtRZF1) in dehydration response. AtRZF1 was significantly reduced by drought stress. The atrzf1 mutant was less sensitive to osmotic stress than the wild-type during early seedling development, wheareas transgenic pants overexpressing AtRZF1 were hypersensitive, indicating that AtRZF1 megatively regulates drought-mediated control of early seedling developmeng. Moreover, the ectopic expression of the AtRZF1 gene was very significantly influential in drought sensitiv parameters including proline content, water loss, membrance ion leakage and the expression of dehy-dration stress-relat genes. ARZF1 is a functional E3 ubiquitin ligase, and its conserved C3H2C3-type RING domain is likely important for the biological function of AtRZF1 in drought response. Together, these results suggest that the E3 ligase AtRZF1 is an important regulator of water deficit stress during early seedling development.

시각의 노화를 고려한 노인종합복지관의 색체계획에 관한 연구

정준수,임환준,이현희,박용환 대한건축학회 2003 대한건축학회논문집 Vol.19 No.7

As human become aged, he become color weak due to yellowed eye sight. The 87% of information for human behavioral decisions are visually perceived. Therefore, the color weakness will affect his behaviors in many ways. Most of previous research regarding color planning for the aged people focuses on discovering their preferred colors and reflecting the colors on facility color planning. But these research failed to identify confused range of color cognitions due to the color weakness of the aged. In this research, targeting the aged in multi-purpose senior centers, we analyze their color distinctions and confusions using computer graphic tools. Our research discovers several important points. First, there are differences in color cognitions between the young facility designers/staffs and the aged facility residents. Second, these differences lead to the differences in the abilities of spatial cognitions between the young and the aged. Third, the difference in the spatial distinctions if mainly caused not by color itself but by color adjacencies. Based upon these findings, we propose that color planning of facilities for the aged be made not from the view of the designers but from the view of the aged, considering yellowed eye sight of the aged.

진화 알고리듬을 위한 객체지향 모델링과 클래스 라이브러리 구현

정호연,이수연,곽재승,김용주,박기태,현철주 한국경영과학회 2000 經營 科學 Vol.17 No.2

In evolutionary algorithm, there exist various models for the evolution of the population with respect to schemes and strategies for reproduction, in the application of the algorithm to a specific problem, one model suitable to the problem is to be properly chosen and a program expert or a software is needed to help implement and test a designed algorithm. In this study, object oriented modeling and the class library for simple evolutionary algorithms(SEA) with one population is developed. The library proposed here can be used as a generalized tool for solving problems in a wide range of domains.

맥주맥 F_1세대의 조합능력 검정

정원복,오주성,황필성,김수동,서세정,현종내,김대호 東亞大學校 2002 東亞論叢 Vol.39 No.-

맥주맥의 이면교잡에 의한 F_1세대에 대한 조합능력을 검정한 결과를 요약하면 다음과 같다. 8개 형질에 대한 GCA, SCA, RCA효과를 검정한 바 조합능력은 전 형질에서 GCA, SCA, RCA가 모두 유의하였는데 간장, 수장, 망장, 간직경, 곡핍폭, 1수립수 1000립중은 GCA가 SCA보다 크고, 품종간 GCA효과는 진양보리가 간장에서 부(-)이고, 삼도보리가 수장·망장·간직경·1000립중에서, 사천6호가 망장과 간직경에서, 진광보리가 곡립장에서, 두산29호가 곡립폭·1수립수·1000립중에서 각각 정(+)으로 높았다. SCA효과에서 수장은 두산29호×두산8호 조합이, 망장은 진양보리×두산29호 조합이, 간직경은 사천6호×진광보리 조합이, 곡립장은 진양보리×두산8호 조합이, 곡립폭은 진광보리×두산8호 조합이, 1수립수는 진양보리×삼도보리 조합이, 1000립중은 사천6호×두산8호 조합 및 두산29호×두산8호 조합이 각각 정으로 높았고, 간장은 삼도보리×진광보리 조합이 부로서 높았다. RCA효과에서 수장은 두산8호×남향보리 조합이, 망장은 진양보리×삼도보리 조합이, 간직겨은 사천6호×진양보리 조합이, 곡립장은 사천6호×진광보리 조합이, 곡립폭은 진양보리×삼도보리 조합이, 1수립수는 사천6호×두산29호 조합이, 1000립중은 진양보리×두산29호 조합이 각각 정으로 높았고, 간장은 사천6호×두산29호 조합이 부로서 높았다. Seven barley varieties in F_1 generation of the possible crosses among them were used to estimate the combining ability for eight characters, i.e., culm length, spike length, awn length, culm diameter, grain length, grain width, number of grains per spike and 1000 grains weight. Data for F_1 hybrid and parents were subjected to the analysis method proposed by Griffing. The results obtained were summarized as follows. mean squares of general combining ability(GCA), specific combining ability(SCA) and reciprocal combining ability(RCA) were significant for all characters observed. Mean squares of GCA were higher than those of SCA and RCA in culm length, spike length, awn length, culm diameter, grain width, number of grains per spike and 1000 grains weight. Variety Dusan#29 showed the highest GCA effect for number of grains per spike and positively high effects of GCA were observed from variety Samdo bori in spike length, awn length, culm diameter, 1000 grains weight, and from variety Jinkwang bori I grain length, from Dusan#29 in grain width, umber of grains per spike, 1000 grains weight. Also Jinyang bori showed negatively high GCA effect in culm length. In SCA effects, hybrids in Jinyang bori × Samdo bori were exhibited positively high for number of grains per spike and high SCA effects for 1000 grins weight were found positively in Sacheun#6×Dusan#8 and Dusan#29×Dusan#. In RCA effects, hybrids in Sacheun#6×Dusan#29 were exhibited positively high for number of grains per spike and high RCA effects for 1000 grains weight were found positively in Jinyang bori×Dusan#29.

급성흉통으로 내원한 환자에서 심근경색증을 조기 발견하기 위한 TROP-T?Rapid Assay의 유용성

정준영,이창현,김현정,도호석,이삼범,도병수 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Study Objectives : In the past, measurement of cardiac enzymes(i.e. CK and/or CK-MB, GOT, LDH)and myoglobin, cardiac specific serum troponin-T were widely used for the diagnosis of acute myocardial infarction, but it need a long time and have low specificity. Thus new diagnostic modality, TROP-T?? Rapid Assay, is very easy and rapid qualitative methods, is needed for the early detection of acute myocardial infarction. Methods : Total CK, GOT, LDH, myoglobin and serum troponin-T activities were measured in patients presenting with acute chest pain at the emergency department and at the same time TROP-T? Rapid Assay was done. Final diagnosis and diagnostic usefulness was evaluated. Results : Overall diagnotic sensitivity and specificity of initial results were as follows; TROP-T? Rapid Assay, 77.3, 100.0; myoglobin, 63.6, 70.4; CK, 95.5, 96.3; GOT, 86.4, 96.3; LDH, 90.9, 92.6; serum troponin-T, 86.4, 96.3, respectively. Conclusion : The TROP-T?? Rapid Assay is simple method and has a short time and nearly same sensitivity and specificity compared to other cardiac enzymes. Thus this is considered useful method in the early detection of acute myocardial infarction at emergency department.

Radiocobalt의 體內 汚染에 對한 除染效果

정인용,정현우,김태환,진수일,윤택구 대한방사선 방어학회 1988 방사선방어학회지 Vol.13 No.1

국내 原子力 産業의 施設增大로 放射線核種汚染의 가능성이 날로 증가되고 있음에도 불구하고 종사자 및 인근주민에 대한 診療對策에 관한 연구가 전무한 실정에 있어 이에 대한 기초자려마련의 일환인 應急處置方案을 수립코자 58CoCl2 1μCi를 마우스 (NIH-(GP))의 腹腔內에 投與한 후 CoNa3 DTPA 8.4mg/0.2ml saline, CoNa3 DTPA 8.4mg/0.2ml saline, saline 5ml등을 각각 投與하였으며, cobalt의 全身殘存量, 體內分布 및 尿內 含有된 量을 測定하기 위해 投與 後 4, 8, 12, 48시간, 그리고 7일에 MCA의 Ge-detector로 放射能을 計測하였고, 또한 각 實質臟器內 殘存된 cobalt의 放射能을 測定하기 위하여 각 group당 6마리의 마우스를 屠殺解體하여 測定하였던바 다음과 같은 결론을 얻었다. CoNa3 DTPA 處置群에서는 汚染된 放射性 cobalt의 全身殘存率의 減少 및 排泄率 增加에 유효한 效果가 있었으며, system contamination에 대한 방어효과는 CoNa3 DTPA, CoNa3 DTPA 그리고 saline 順으로 유효하였다. 결론적으로 본 실험결과로 볼 때 放射性 cobalt의 體內汚染에 대한 緊扱措置는 CoNa3 DTPA와 다량의 물을 동시에 投與함으로써 體內汚染된 放射性 cobalt의 排泄을 促進시킬 것으로 사료된다. In case of the acute intake of radionuclide, an early medical treatment may be necessary, but the little is established the procedures to decontaminate the victims of internal contamination in Korea. The purpose of the present investigation is to study chemical agents to remove radiocobalt from the victims and to provide a more reliable procedure for the treatment. The removals of radiocobalt from the NIH-(GP)mice injected intraperitoneally with lμCi of 58Co as CoCl2 were investigated with doses of either CaNa3 DTPA 8.4mg/0.2ml saline, CoNa3 DTPA 8.4mg/0.2ml saline, or saline 5ml. The radioactivity was determined by MCA and Ge-detector on 4, 8, 12, 48 hours and 7 days for the whole body, organ distribution and urine excretion. Six mice per each group were sacrificed for the measurement of cobalt retention in the parenchymal tissue. The cobalt trisodium chelate had a pronounced effect on reducing the whole body retention and increasing the excretion rate. Regarding to the systemic protective effects, CoNa3 DTPA, CaNa3 DTPA and saline were effected significantly in order. In conclusion, the extrapolations from these results to human were suggested that the rapid administration of cobalt trisodium chelate and an amount of saline to the contaminated person after internal contamination of radiocobalt were markedly increasing the decontamination effects.

역행성 골수강내 금속정을 이용한 대퇴골 과상-과간 골절의 치료

정필현,황정수,강 석,김종필,전용진 대한골절학회 2004 대한골절학회지 Vol.17 No.2

단일경사열선을 이용한 3차원 유동장의 자동측정방법

정의준,김무현,김수태 울산대학교 1992 공학연구논문집 Vol.23 No.2

퍼스널 컴퓨터 및 스텝핑 모터를 사용한 자동계측장치를 개발하였고 이러한 자동계측장치를 이용하여 단일경사열선으로 유동장의 3차원 속도성분을 측정하였다. 검정실험을 통해 얻어진 검정실험식의 사용범위를 알아보기 위해 검정실험식의 사용범위를 알아보기 위해 검정실험식으로부터 구한 속도, 스윌각 및 피치각들을 실제 측정값들과 비교 검토하였다. The 3-D velocity components were measured by single slanted hot wire. An automatic measuring system which was controlled by means of a personal computer and stepping motors was developed. Absolute velocity, swirl angle and pitch angle are calculated from the outputs of hot wire anemometer using the calibration equations. The difference between the measured and calculated values was investigated.