Progress in the development of heating systems towards long pulse operation for KSTAR

Kwak, J.G.,Bae, Y.D.,Chang, D.H.,Chang, D.S.,Hong, B.G.,Hwang, C.K.,In, S.R.,Jeong, S.H.,Jin, J.T.,Jung, K.S.,Kim, B.R.,Kim, J.,Kim, S.K.,Kim, T.S.,Lee, D.W.,Lee, K.W.,Oh, B.H.,Seo, C.S.,Seo, M.S.,Yoo International Atomic Energy Agency 2007 Nuclear fusion Vol.47 No.5

<P>Construction of the Korea superconducting tokamak advanced research (KSTAR) tokamak is in its final phase. For the long-pulse KSTAR discharges, the ion cyclotron range of frequencies (ICRF) and neutral beam injection (NBI) heating systems are expected to play important roles through a selective heating of ions and electrons, control of the plasma pressure and current profiles, a core fuelling and beam diagnostics for the KSTAR. In addition, the ICRF system is expected to be used for possible discharge cleaning and assisting in the tokamak startup. In this paper, the recent progress in the development of the ICRF and the NBI heating systems is described. The four-strap ICRF antenna has been successfully tested for a voltage up to 41 kV for a pulse length of 300 s (to 46 kV for 20 s) in a test chamber. A prototype KSTAR NBI system has been developed. At present, the system has successfully produced a 1 MW beam power for 200 s and a 3.5 MW output beam power for 4 s.</P>

간호 대학생의 성 지식, 성 태도와 성 건강 간호 장애감 간의 관계

김다연,김은아,노수경,류아인,박나은,박지혜,서정민,심효정,주성민,한진실,이건정,박수민 이화여자대학교 간호과학대학 2017 이화간호학회지 Vol.- No.51

Purpose: The purpose of this study was to investigate correlation among sexual knowledge, attitude and Barriers to patient’s sexual health of nursing undergraduate students and to provide objective data to promote sexual health-care. Method: Using a descriptive correlation, 304 nursing undergraduate students were recruited through convenience random sampling from August 1st to December 20th, 2016. Data were analyzed using average, standard deviation, percentage, t-test, ANOVA, Scheffe test, Pearson’s correlation and Multiple linear regression analysis using the SPSS 21.0 program. Results: The correlation between Sexual knowledge and Sexual attitude is statistically significant(r=.289, p<.01). As the level of sexual knowledge is higher, the sexual attitude is more open. And, Sexual knowledge(r=-.307,p<.01) and Sexual attitude(r=.180,p=.002) had a negative relationship with Barriers to patient’s sexual health. Also, Sexual knowledge was the greatest influencing factor on Barriers to patient’s sexual health. Conclusion: This study showed as the level of Sexual knowledge is higher, the Sexual attitude is more open. As the degree of Sexual knowledge is lower, the Sexual attitude is more conservative, and Barriers to patient’s sexual health is more higher. Also, Sexual knowledge is the most influential factor on Barriers to patient’s sexual health.

Sphingosine-1-phosphate Signaling in Human Submandibular Cells Sphingosine-1-phosphate (S1P) triggers Ca²⁺ signaling and the apoptotic pathway in normal submandibular gland cells, which suggests in turn that S1P affects the progression of Sjog

Seo, J.,Koo, N.-Y.,Choi, W.-Y.,Kang, J.-A.,Min, J.H.,Jo, S.-H.,Lee, S.J.,Oh, S.-B.,Kim, J.-S.,Lee, J.-H. AMERICAN AND INTERNATIONAL ASSOCIATIONS FOR DENTAL 2010 Journal of dental research Vol.89 No.10

The effects of mesenchymal stem cells injected via different routes on modified IL-12-mediated antitumor activity

Seo, S H,Kim, K S,Park, S H,Suh, Y S,Kim, S J,Jeun, S-S,Sung, Y C Nature Publishing Group 2011 Gene Therapy Vol.18 No.5

<P>Owing to its tumor tropism and prolonged transgene expression, mesenchymal stem cell (MSC) has been considered as an ideal delivery vehicle for cancer gene therapies or therapeutic vaccines. In this study, we demonstrated that intratumoral (i.t.) injection of MSCs expressing modified interleukin-12 (MSCs/IL-12M) exhibited stronger tumor-specific T-cell responses and antitumor effects as well as more sustained expressions of IL-12 and interferon (IFN)-γ in both sera and tumor sites than did IL-12M-expressing adenovirus (rAd/IL-12M) in mice bearing both solid and metastatic tumors. Subcutaneous (s.c.) injection of MSCs/IL-12M at contralateral site of tumor exhibited similar levels of serum IL-12 and IFN-γ as i.t. injection, but much weaker antitumor effects in both B16F10 melanoma and TC-1 cervical cancer models than i.t. injection. Although intravenous (i.v.) injection elicited earlier peak serum levels of cytokines, it induced weaker tumor-specific T-cell responses and antitumor effects than i.t. injection, indicating that serum cytokine levels are not surrogate indicators of antitumor effects. Taken together, these results indicated that MSC is more efficient than adenovirus as a cytokine gene delivery vehicle and that i.t. injection of MSCs/IL-12M is the best approach to induce strong tumor-specific T-cell responses that correlate with anti-metastatic effects as well as inhibition of solid tumor growth, although MSCs themselves have an ability to migrate into the tumor site. In addition, MSCs/IL-12M embedded in Matrigel (MSCs/IL-12M/Matrigel) exhibited significant antitumor effects even in immunodeficient mice such as SCID and BNX mice lacking T, B and natural killer (NK) cells, but not in IFN-γ knockout mice. Our findings provide an optimal approach for designing an efficient clinical protocol of MSC-based cytokine gene therapy to induce strong tumor-specific T-cell responses and therapeutic anticancer efficacy.</P>

Purification and characterization of a novel fibrinolytic α chymotrypsin like serine metalloprotease from the edible mushroom, Lyophyllum shimeji

Moon, S.M.,Kim, J.S.,Kim, H.J.,Choi, M.S.,Park, B.R.,Kim, S.G.,Ahn, H.,Chun, H.S.,Shin, Y.K.,Kim, J.J.,Kim, D.K.,Lee, S.Y.,Seo, Y.W.,Kim, Y.H.,Kim, C.S. Society for Bioscience and Bioengineering, Japan ; 2014 Journal of bioscience and bioengineering Vol.117 No.5

A novel fibrinolytic enzyme was purified from Lyophyllum shimeji, a popular edible mushroom in Asia. The enzyme was purified using combination of anion exchange chromatography on a Mono Q 5/5 column and size exclusion gel filtration chromatography on Superdex 200 100/300 column. This purification protocol resulted 80.9-fold purification of the enzyme and a final yield of 5.7%. The molecular weight of the purified enzyme was estimated to be 21 kDa by SDS-PAGE and size exclusion gel filtration. The N-terminal amino acid sequence was found to be ITFQSASP, which is dissimilar from that of known fibrinolytic enzymes. The purified enzyme was a neutral protease with an optimal reaction pH and temperature of 8.0 and 37<SUP>o</SUP>C, respectively. Enzymatic activity was inhibited by Cu<SUP>2+</SUP> and Co<SUP>2+</SUP>. It was also significantly inhibited by PMSF and TPCK. Furthermore, it was found to exhibit a higher specificity for S-7388, a well-known chymotrypsin chromogenic substrate, indicating chymotrypsin like serine metalloprotease. The relative fibrinolytic activity of 5 μg purified enzyme have two fold more activity than 1 unit/ml of plasmin on fibrin plate. Furthermore, purified enzyme preferentially hydrolyzed the Aα-chain followed by the Bβ- and γ-chain of fibrinogen, which is precursor of fibrin. Therefore, these data suggests that the fibrinolytic enzyme derived from edible mushroom, L. shimeji, might be useful for thrombolytic therapy and preventing thrombotic disease.

Rosiglitazone-mediated dendritic cells ameliorate collagen-induced arthritis in mice

Byun, S.H.,Lee, J.H.,Jung, N.C.,Choi, H.J.,Song, J.Y.,Seo, H.G.,Choi, J.,Jung, S.Y.,Kang, S.,Choi, Y.S.,Chung, J.H.,Lim, D.S. Pergamon Press 2016 Biochemical pharmacology Vol.115 No.-

<P>Rosiglitazone is a selective ligand for peroxisome proliferator-activated receptor-gamma (PPAR-gamma), which serves diverse biological functions. A number of autoimmune disease models have been used to examine the anti-inflammatory and immunosuppressive effects of tolerogenic dendritic cells (tDCs). The aim of the present study was to investigate whether rosiglitazone-mediated DC (Rosi-DC) therapy suppressed arthritis in a collagen-induced arthritis (CIA) mouse model. Rosi-DCs were generated by treating immature DCs with TNF-alpha, type II collagen, and rosiglitazone. CIA mice then received subcutaneously (s.c.) two injections of Rosi-DCs. The severity of arthritis was then assessed histopathologically. The phenotypes of the DC and regulatory T (Treg) cell populations in CIA mice were determined by flow cytometry and the effect of Rosi-DCs on the secretion of autoimmunity-inducing cytokines was examined by ELISA. Rosi-DCs expressed lower levels of DC-related surface markers than mature DCs. Histopathological examination revealed that the degree of inflammation in the paws of Rosi-DC-treated mice was much lower than that in the paws of PBS-treated CIA mice. Taken together, these results clearly show that rosiglitazone-mediated DCs ameliorate CIA, most likely via the induction of antigen-specific Treg cells. (C) 2016 Elsevier Inc. All rights reserved.</P>

간호대생과 의대생의 간호사 이미지 비교

구민진,김수영,방정민,서아영,양희진,윤소람,이윤재,이재은,이지연,정윤경,최수정 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48

Purpose: This study aims to analyze the difference in the perception that nursing students and medical students have regarding the image of nurses. Method: The sampling group for this study was made up of 111 nursing students and 117 medicine students, conducted from the 19th August 2013 to 3rd September 2013. The tool used for this study is the “Nurse Image Scale”. The data is analyzed using the SPSS 21.0 program, technical stats, t-test and ANOVA with Scheffe test. Result: There was a notable difference in the results(t=6.94, p<001), with the average image perception score of nurses at 3.84±0.34 amongst nursing students being higher than the 3.50±0.38 amongst Medicine students. The average score of the 4 areas tested, “Qualification of a Nurse”, “Role of a Nurse”, “Social Participation of a Nurse” and “Interpersonal Skills of a Nurse” were all marked higher by the nursing students than the medicine students. The average score became notably higher as the period of practice became shorter with nursing students (F=4.21, p=.043). Furthermore, the average score for the “Qualification of a Nurse” was notably higher as the period of practice became shorter (F=3.98, p=.049). Medical students gave an average score for the “Qualification of a Nurse”(F=3.72, p=.027) and the “Interpersonal Skills of a Nurse”(F=4.11, p=.019) which was relative to the development of a nurse’s image, while the average score for the “Role of a Nurse” was notably higher with a longer period of practice(F=6.65, p=.011). Conclusion: The results of this study suggest that the image perception of a nurse can vary depending on the experience in period of practice. Therefore, together with this study conducted with nursing students and medicine students, there is a need for further studies conducted on image perception of nurses with various experience in period of practice.

Protective efficacy of Streptococcus iniae derived enolase against Streptococcal infection in a zebrafish model

Membrebe, J.D.,Yoon, N.K.,Hong, M.,Lee, J.,Lee, H.,Park, K.,Seo, S.h.,Yoon, I.,Yoo, S.,Kim, Y.C.,Ahn, J. Elsevier 2016 Veterinary immunology and immunopathology Vol.170 No.-

<P>Enolase (ENO) is one of the surface-exposed proteins of Streptococcus iniae, which previously had been identified as a plasminogen-binding protein. In this study, ENO was evaluated to induce cross-protective immunity against S. iniae and Streptococcus parauberis which are major pathogens causing streptococcosis in fish. Immunoblot analysis shows that S. iniae recombinant ENO (rENO) produced in Escherichia coli was cross-reactive with antisera against S. iniae, and S. parauberis serotype land II. In the challenge experiment of streptococcal infection after vaccination in zebrafish, rENO elicited a similar protection with a whole cell bacterin against S. iniae and S. parauberis, which suggests its feasibility as an efficient vaccine against streptococcosis. (C) 2016 Elsevier B.V. All rights reserved.</P>

남녀공학과 여대에 재학 중인 여자대학생의 체형만족도와 자아존중감의 관계

김민정,김보민,김소연,김형주,서민경,박수영,전승연,한누리,함승순 이화여자대학교 간호과학대학 2011 이화간호학회지 Vol.- No.45

Purpose: This study was done to investigate the relationship between satisfaction of body figure and self-esteem of female university students according to the school types. Method: The study sample consisted of 360 female university students who had been attending at co-educational university or women's university in Korea. Data were collected by questionnaires from July 27th to August 6th in 2010. All statistical analyses were conducted using t-test, chi-square test, Fisher's exact test, ANOVA, Kruskal Wallis test, and Pearson’s Correlation Coefficient procedures with SPSS 18.0 program. The level of significance was set at .05. Results: The general characteristics, satisfaction of body figure and self-esteem were not significantly different between co-educational university and women’s university. Although 93.1 percent of female students were under or normal weight according to their BMI, 88.0 percent of female university students were not satisfied with their current body figure and 90.5 percent among them wanted to lose weight. The satisfaction of body figure was related with self-esteem (coed: r=-0.228, p=.002, women's: r= -0.264, p<.001) but there was no difference from school types. Conclusion: The result implied the importance of appropriate education and nursing intervention which would improve satisfaction of body of female university students.

PCR 기법을 이용한 한국재래산양의 α s2-casein 유전자의 특성

이상훈,상병찬,한성욱,김지애,서길웅 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from the analysis of genetic characteristics of α_(S2-) casein gene in Korean Native goat using PCR-RFLP. This study confirmed the amplified product of 1.3kb fragment obtained from the amplification of a α_(S2-)casein loci by PCR. The frequency of a α_(S2-)casein genotype in Korean Native goat was 100% for AA type and the frequencies of a α_(S2-)casein genotype in Saanen were 80 and 20% for AA and AB genotypes. The frequency of a α_(S2-)casein A allele was 1.000 in Korean Native goat, and the frequencies of a α_(S2-)casein A and B alleles were 0.900 and 0.100 in Saanen, respectively. The nucleotide sequence of a α_(S2-)casein gene of Korean Native goat was 99.0% homology with 12 nucleotide sequences difference of that of goat reported in GeneBank(AJ238965.1, AJ238967.1) and was 95.0% homology with 57 nucleotide sequences difference of that of Holstein reported in Gene Bank(M94327). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphisms and sequences for a α_(S2-)casein gene should be used as basic data for preservation and improvement of genetic resources in Korean Native goat breeding.