독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

Pulse YAG Laser 제어 시스템에 의한 공구강의 조직 특성에 관한 연구

옥철호,서영백,배춘익,박흥식,전태옥 동아대학교 생산기술연구소 1999 生産技術硏究所硏究論文集 Vol.4 No.2

Case Organization Characteristics of tool steel (SK5) was investigated after Pulse YAG laser control system irradiation. In the case of beam passes, martensite formed in the melt zone and in former pearlite regions of the austenization zone exhibited very high vickers hardness values. Molten depth and width depend on defocusing distance and radius, micro structure, hardness were investigated as a function of defocusing distance, pulse width, power density and black color painting have a energy absorption more than other any color painting.

엔드밀 고속평면 가공시 표면형상 해석

배효준,이상재,서영백,박흥식,전태옥 동아대학교 생산기술연구소 2002 生産技術硏究所硏究論文集 Vol.7 No.1

High speed machining system have been used in industrial, because it is effective that a material manufactured with various shape. Recently a end-milling processing is need the high-precise technique with good surface roughness and rapid time in aircraft industry, automobile part and molding. Therefore this study proposed to decide best manufacturing cutting condition for surface roughness and rapid manufacturing time by using computer image processing system and 3D modeling. Until the 16,000 rpm, the surface roughness is decreased rapidly, but it is not over that. The 22,000 rpm is the spindle speed with the optimum surface in the high speed end-milling. In the case of the feed rate with 2,000 mm/min and 8,000 mm/min, the surface roughness is better than 4,000 mm/min and 6,000 mm/min. By using the 3D modeling, it is effectively represented shape characteristics of working surface in high speed end-milling.

마찰조건의 판정을 통한 기계습동면의 Condition Monitoring

조연상,김동호,서영백,박흥식,전태옥 동아대학교 생산기술연구소 1999 生産技術硏究所硏究論文集 Vol.4 No.1

Wear particles can be collected from the lubricants of machinery and its morphology is directly related to the damage to the interacting surfaces from which the particles originated. The morphology of the wear particles on friction condition are therefore directly indicative of wear processes occuring in machinery. This paper was undertaken to condition monitoring of the lubricated machine surface through decision of friction condition by neural network model. In order to describe the characteristics of wear debris with various shapes and sizes, the four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) of wear particles on friction condition are used as inputs to the network and learned the friction condition of five values.

Fractal 차원을 이용한 마멸입자의 형태인식에 관한 연구

오동석,조연상,서영백,박홍식,전태옥 동아대학교 생산기술연구소 1999 生産技術硏究所硏究論文集 Vol.4 No.1

The morphological identification of wear particles is a very effective means for machine condition monitoring and fault diagnosis. In order to describe morphology of various wear particles, the wear test was carried out under different experimental conditions. And fractal descriptors was applied to boundary and surface of wear particles with image processing system These descriptors to analyze shape and surface wear particles are shape fractal dimension and surface fractal dimension. The shape fractal dimension can be derived from the boundary profile and surface fractal dimension can be determined by sum of intensity difference of surface pixel. The morphological identification of wear particles can be effectively obtained by two fractal dimensions.

전경대원에서 발생한 결핵의 임상 양상

문창기,박상준,조민구,김영중,김소연,김윤권,정준오,안석진,김은실,서승오,김지훈,최원제,이윤영,박형기,최규영,김현근 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background: The purpose of this study was to document the incidence and clinical characteristics of patients with tuberculosis (TB) in combat and auxiliary police, living in a group, in Korea where the incidence rate of active TB in a general population is higher than in Western countries. Materials and Methods:We retrospectively reviewed the medical records of all tuberculosis patients diagnosed at National Police Hospital from January 2002 through December 2004. Results:In 2002-2004, a total of 156 cases of tuberculosis were identified with the mean (Standard deviation) age of 20.6 (±1.0) years. Of these, 134 (85.9%) patients were registered as new cases, 11 (7.1%) as relapse, 2 (1.3%) as failure, 5 (3.1%) as treatment-after-default cases whereas 4 (2.6 %) patients were not included in any categories. Average annual new TB rate and smear-positive TB rate were 86.5/10^(5) and 17.4/10^(5) person-years, respectively. In 12 of 31 smear-positive cases, time from onset of symptoms to diagnosis was more than 30 days. Two multidrug-resistance TB cases were identified and two suspected outbreak episodes of TB had occurred during 3 years. Conclusion:There was no statistically significant difference in the incidence rate of new cases of TB between the general population aged 20 to 29 years and combat and auxiliary police in Korea. 배경 : 국내 결핵의 발생은 감소 추세에 있으나 여전히 후진국형 발생양상을 보이고 있다. 특히 집단생활을 하는 젊은이들에서 발생하는 결핵의 유행이 간헐적으로 알려지고 있어 우려를 낳고 있다. 이에 저자들은 최근 전경대원의 결핵 발병 양상에 대해 알아보고자 본 연구를 시행하였다. 재료 및 방법 : 2002년 1월부터 2004년 12월까지 3년간 경찰병원에서 결핵 진료를 받은 전경대원의 의무기록을 후향적으로 분석하여 연도별 결핵 신환발생률, 도말양성결핵 신환율, 진단 지연, 집단발병, 다제내성결핵을 조사하였다. 결과 : 연도별 결핵 신환자 수(10만명당 발생추정치)는 2002년 50명(83.6명), 2003년 42명(83.7명), 2004년 42명(93.5명)이었고 도말양성 폐결핵 신환자 수는 2002년 10명(16.7명), 2003년 11명(21.9명), 2004년 6명(13.4명)이었다. 3년간 도말양성 폐결핵 환자는 31명이 있었고 그 중 12명은 호흡기 증상 발생 30일이 지나서 진단을 받았다. 다제내성 결핵은 2명이 있었으며 결핵의 집단발병이 의심되는 사례는 2차례 있었다. 결론 : 전경대원과 20-29세 연령군 일반인의 결핵 신환발생률, 도말양성 폐결핵 발생률의 통계적으로 유의한 차이는 없었다.

기계구동계의 손상상태 진단을 위한 컴퓨터 영상해석

서영백,김동호,이광영,박흥식,전태옥 동아대학교 공과대학부설 생산기술연구소 1998 生産技術硏究所硏究論文集 Vol.3 No.1

Microscopic examination for the morphological estimation of wear debris on the oil-lubricated moving system is an accepted method for machine condition and fault diagnosis. However wear particle analysis has not been widely accepted industry because it is dependent on expert interpretation of particle morphology and relies on subjective assessment criteria. This paper was undertaken to estimate the morphology of wear debris on the oil-lubricated moving system by computer image analysis. The wear test was performed under different sliding conditions using a wear test device made in our laboratory and wear testing specimen of the pin-on-disk-type was rubbed in paraffine series base oil. In order to describe characteristics of debris of various shape and size, four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) have been developed and outlined in the paper. A system using such techniques promises to obviate the need for subjective, human interpretation of particle morphology in machine condition monitoring.

단서자극과 표적자극의 표상된 위치의 일치성이 주의기제의 작용에 미치는 영향

서준호(Jun-Ho Seo),이형철(Hyung-Chul O. Li) 한국인지과학회 2009 인지과학 Vol.20 No.4

본 연구의 목적은 단서와 표적의 표상된 위치의 일치성이 주의기제의 작용에 중요한 역할을 하는지 회귀억제 연구방법을 이용하여 규명하는 것이었다. 실험 1에서는 청각단서와 시각표적을, 실험 2에서는 시각단서와 청각표적을 사용하였고, 단서와 표적이 제시되는 표상된 위치와 물리적 위치의 일치성에 따라 일치조건과 불일치조건으로 구분하였다. 실험결과, 실험 1에서 표상된 위치의 일치성에 따라 일치조건과 불일치조건을 구분하였을 경우 일치조건에서 반응촉진이 관찰되었다. 하지만 물리적 위치의 일치성에 따른 일치/불일치조건에서는 반응시간 차이가 관찰되지 않았다. 실험 2에서도 표상된 위치의 일치성에 따라 일치조건과 불일치조건을 구분하였을 경우 일치조건에서 반응촉진이 관찰되었으나 매우 제한적이었다. 이러한 실험결과는 단서와 표적의 표상된 위치의 일치성이 주의기제의 역할 중 반응촉진에 중요한 역할을 하며, 주의 연구에서 물리적 위치와 표상된 위치를 구분해야할 필요성을 시사한다. The purpose of the present research was to examine whether the attention mechanism employs physical or represented location of the cue and target. To achieve this, we have employed the paradigm of facilitation of response as well as inhibition of return. In the experiments, valid and invalid conditions were defined by the position consistency of the cue and the target in the aspect of either physical or represented location. We used auditory cue and visual target in Experiment 1 while visual cue and auditory target in Experiment 2. As a results, in Experiment 1, effect of facilitation of response in valid condition was found when the valid/invalid conditions were defined in the aspect of represented location. In Experiment 2, effect of facilitation of response in valid condition was found when the valid/invalid conditions were defined in the aspect of represented location. In all the other conditions, no effect was found when the conditions were defined in the aspect of physical location. No effects of inhibition of return were found in Experiment 2. These results imply the possibility that attention mechanism operates based on objects' represented location rather than on their physical location. More importantly, the present research suggests that it is necessary to separate represented location from physical location of the target and the cue in the experiment of facilitation of response and inhibition of return in the future.

Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats

Kwon, O Jun,Kim, Min Yeong,Shin, Sung Ho,Lee, Ah Reum,Lee, Joo Young,Seo, Bu-il,Shin, Mi-Rae,Choi, Hyun Gyu,Kim, Jeong Ah,Min, Byung Sun,Kim, Gyo-Nam,Noh, Jeong Sook,Rhee, Man Hee,Roh, Seong-Soo Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by<I> in vitro</I> 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO<SUP>−</SUP>). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-<I>κ</I>B) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis<I> via</I> regulating NF-<I>κ</I>B mediated inflammation related to oxidative stress. </P>

컴퓨터 비전 기술을 활용한 건설 안전보건 관리

서준오,Seo, Jun-O 한국건설관리학회 2016 건설관리 : 한국건설관리학회 학회지 Vol.17 No.4