Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

설악산국립공원 분비나무 서식지의 경관지수 분석^a

박홍철 ( Hong-chul Park ),이호 ( Ho Lee ),김윤영 ( Yoon-young Kim ),이나연 ( Na-yeon Lee ) 한국환경생태학회 2017 한국환경생태학회 학술대회지 Vol.2017 No.1

내사시에서 양안 내직근후전술 시 기능적적도부 및 팍스의 수술양을 고려한 군 간 수술성적비교

강병완,박상우,윤경철,허환,Byung Wan Kang,MD,Sang Woo Park,MD,PhD,Kyung Chul Yoon,MD,PhD,Hwan Heo,MD,PhD 대한안과학회 2012 대한안과학회지 Vol.53 No.1

Purpose: To compare the surgical outcomes of functional equator-considering and Parks’ methods in infantile esotropia and partially accommodative esotropia. Methods: The medical records of 96 patients with infantile esotropia and partially accommodative esotropia who underwent bilateral medial rectus muscle recession by functional equator-considering and Parks’ methods were reviewed retrospectively. The surgery success rate at 1 month, 3 months, 6 months and 12 months was compared by the deviation angle measurement. Results: Overall success rates were not statistically different between the 2 groups. Additionally, there were no differences in surgical success rate of functional equator-considering and Parks’ methods in infantile esotropia and partially accommodative esotropia. When the patients were divided into 2 groups based on 40 PD, no significant difference in success rate between the 2 groups was observed. When patients were divided into 2 groups based on 22 mm of axial length, there was no statistical difference observed. Postoperative overcorrection was 2 (4%) in the functional equator group and 3 (6.5%) in Parks’ method group and postoperative undercorrection was 9 (18%, 19.6%) in each group. The incidence of undercorrection in the Parks’ method group was significantly higher in the greater than 22 mm axial length group (7, 35.0%) than in the less than 22 mm group (2, 7.7%) (p = 0.029). Conclusions: There was no statistically significant difference in surgical success rate between the functional equator and Parks’ methods in infantile esotropia and partially accommodative esotropia. J Korean Ophthalmol Soc 2012;53(1):138-144

한국인에서의 무과립구증 발병율 조사연구 -제4상 임상시험을 위한 기초자료조사-

안윤옥,박병주,이무송,김병국,구홍회,서철원 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

열대 지역에서 통일형 초다수성 벼 품종의 생육 및 수량성 비교

이지윤,준현,성환,손영보,송유천,한상익,박동수,권영업,이철원,이종희 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

수술적 방법을 이용한 안면부 거대혈관종의 치료

박재구,이윤호,김진환,민경원,박철규,권성택,김석화 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.6

Hemangioma is one of the most common congenital tumors in the region of the face and neck. Although histologically benign, these facial masses are clinically malignant for their deforming and inexorable growth, especially in so-called cavernous hemangioma. Today, hemagiomas have been treated by various methods; steroids, electrocoagulation, injection of sclerosing agent, cryotherapy, radiation therapy, laser therapy, and surgical treatment, etc. The response of treatment varies with the types, surgical skills, regions of hemangioma, the age of patient, but the appropriate method of the treatment has not been yet established. In the case of massive hemangioma, which involves most part of the face and neck, we prefer surgical treatment and consistently obtain better result than other treatment modalities. Wide excision and proper reconstruction of the face helps the patient for their better life aesthetically and functionally.

환자에서 분리한 Klebsiella pneumoniae의 장독소 생성능 및 활성에 대하여

윤정중,이태연,박철희 단국대학교 신소재기술연구소 1997 신소재 Vol.7 No.-

임상에서 가져온 99주에 대한 재 실험 결과 40주의 분리균은 생물, 화학적 성상이 Klebsiella pneumoniae ATCC 13883과 동일한 성상을 나타내었으며, K99 균주의 장독소 생성능을 CYE 배지와 CAYE 배지에서 비교하였을 때, CYE 배지에서의 독소 생성능이 높았다. 또한 균주의 배양시간별 장독소 생성능 측정을 위해 배양 후 12시간부터 24시간까지 3시간 간격으로 측정해 본 결과, 21시간 배양했을 때 장독소 생성능이 가장 높았고 마우스 접종시간별 장 독성능을 알아보기 위해 접종 후 시간별로 장 독성능을 측정해 본 결과, 접종 후 4시간후에 장 독성능을 측정했을 때의 효과가 가장 높았다. 따라서 이상의 결과를 종합하면, 환자로부터 분리한 Klebsiella pneumoniae K99 균주는 기존에 보고된 것과는 다소 차이가 있는 생물 및 생화학적 특징을 나타내는 장독소를 생산하는 것을 알 수 있었다. In this study, the enterotoxin of Klebsiella pneumoniae isolated from clinical patients in Korea was characterized. Enterotoxin of Kelbsiella pneumoniae has been known to be secreted in the water and electrolytes in the small intestines of human resulted in diarrhea. For this reason, Klebsiella pneumoniae strains were isolated from patients at hospitals in Seoul, Korea and were investigated for their ability for enterotoxin production by the method of suckling mouse assay. The results were as follows; 1. For their ability of enterotoxin production by the culture media, the casamino acid yeast extract medium was more effective than the casamino acid yeast extract salt medium. 2. For their ability of enterotoxin production by the culture time, enterotoxin their peaked at 21 hrs of incubation. 3. Maximal cytotoxicity was achieved at 4 hrs after the administration of the enterotoxin into the mouse.

주거지역 오수발생 특성 및 원단위 산정

박철휘,양경수,배윤선 대한상하수도학회 2002 상하수도학회지 Vol.16 No.4

The purpose of this study is to investigate the quantity of discharged sewage unit loading factor and the quality of various pollutants in domestic (detached house and apartment) and commercial site. Measurement were worked at Bucheon and Seogwipo city. One was selected because it was a developed lately community, the other was chosen because it was constructed for long period from 1973 to 1992. The sewage was sampled every two hours a day for a week per each season. Data for the quantity of used water, the number of population and areas were also investigated. The result of the study were as follows; (1) The average unit water use flowrate were 215.7l pcd for detached house, 200.6l pcd for apartment, 8.9l/㎡/d for commercial site in Bucheon city, and 212.5l pcd for detached house, 187.7l pcd for apartment, 9.7l/㎡/d for commercial site in Seogwipo city. (2) The average unit discharged sewage flowrate were 179.7l pcd for detached house, 187.4l pcd for apartment, 8.1l/㎡/d for commercial site in Bucheon city, and 159.4l pcd for detached house, 153.1l pcd for apartment, 5.6l/㎡ /d for commercial site in Seogwipo city. (3) The conversion ratio from water to sewage were 83.4% for detached house, 93.4% for apartment, 91.8% for commercial site in Bucheon city, and 75.0% for detached house, 82.2% for apartment, 57.5% for commercial site in Seogwipo city. (4) The average unitloading factor of pollutant were COD_cr 44.4 gpcd, BOD_5 23.3 gpcd, SS 14.3 gpcd, T-N 6.7 gpcd, T-P 0.8 gpcd for detached house, COD_cr 62.1 gpcd, BOD_5 29.5 gpcd, SS 21.4 gpcd, T-N 7.0 gpcd, T-P 0.8 gpcd for apartment. (5) The maximum sewage generation based on factors (water supply, running rate, valid water rate and etc.) could be predicted and compared with survey data.

혈뇨의 감별진단을 위한 이형적혈구의 형태학적 연구

윤철종,박정오,문희주,윤기은 韓國電子顯微鏡學會 1999 Applied microscopy Vol.29 No.3

전체 성인의 약 4%에서 정도 차이는 있지만 혈뇨가 있다고 볼 때 그 원인을 구명하는 일이 중요하다. 그 원인이 사구체성 신염인지 기타 그 외의 비사구체성 원인에 따른 질환인지를 쉽게 감별하는 일이 중요하다. 본 연구자들은 혈뇨를 동반한 소아과 환자중에서 신 생검 조직검사까지 한 5명의 환자에서 혈뇨를 표본 제작하여 먼저 위상차현미경적 표본제작법을 통하여 적혈구를 감별하였고 방법을 달리하여 주사형 전자현미경으로 요침사를 관찰하여 형태학적 고찰을 통하여 이형적혈구를 보다 명확한 3차원구조를 관찰할 수 있었다. 침생검을 통해 얻은 조직학적 소견으로는 사구체의 기저막을 통과한 혈구들의 요로를 통하여 배출과정을 광학현미경 및 투과형 전자현미경으로 확인할 수 있었다. 본 연구에서 육안적 혈뇨의 색은 유출되는 적혈구의 양과 상관관계가 있으며 용혈된 정도가 심한 경우에 콜라색 혈뇨가 있음을 알 수 있었다. 이들 결과에서 본 연구자들은 사구체성 질환을 동반한 유극적혈구 및 환형 적혈구 등과 같은 변형된 적혈구를 주사형 전자현미경으로 명확한 관찰을 함으로 사구체성 질환으로 인한 혈뇨의 감별에 주사형 전자현미경이 가장 유용한 검사 방법이라고 생각된다. Examination of the morphology of red blood cells in the urine has been shown to be a promising adjunct in determining whether hematuria represents glomerular or nonglomerular bleeding. This is due to distortion of RBCs as they pass across the basement membrane of the glomerular capillaries. It is concluded that is method can greatly help the clinician in distinguishing between glomerular and non glomerular bleeding in patients with hematuria and channeling such patients toward the most appropriate investigations. We have experimented dysmorphic red blood cells that 5 patients of the hematuria are distorted with irregular outlines and often have small blebs extruding from the red cell membrane. Tried urinary sediments were seen with phase contrast microscope and confirmed scanning electron microscope. There are seen acanthocytes, anulocytes, ghost cells and sphero-echinocytes in dysmorphic erythrocytes. Clinical diagnosis was referred from the result of the biopsy-proven. Scanning electron microscopic findings of the hematuria are good diagnostic tool that disclose in distorted red blood cells from patients with glomerular disorders.

주제학습을 통한 ICT 활용 교육 모형 개발

박선주,김정랑,김철,이윤형 光州敎育大學校 初等敎育硏究所 2002 初等敎育硏究 Vol.17 No.-

In coming 21st century, which is forecasted that it will be a knowledge-information oriented society, abilities of information acquirements are required. For this, the Ministry of Education has been encouraging the business of information oriented school education and promoting application of ICT more than 10% in all classes of the 7th curriculum. Moreover, after extending applied classes into 20% by 2005, the Ministry of Education will promote editing textbooks by subjects reflecting the content of ICT application. But, actually, classes applied ICT aren't fulfilled in education fields. The main reason for this is that teachers don't understand fully ICT application model. On the basis of the preceding studies, this study designed subject-settlement class model to develop students' abilities of information acquirements. The subject-settlement class model is made up of 5 steps--confirmation of studying subjects, searching information and collecting, using information, arrangement of results, communication of information, and evaluation. By experiencing search of information, arrangement of information and creation of new information, the application of this model helps the students develop abilities of information acquirements. Nowadays, development of information communication and of computer makes the world change from information oriented society into knowledge oriented society. Future society will focus on the ability of production to create knowledge information. This study is the result presented as a part of the ability of creating information. Furthermore, the development of various learning and teaching models, which are required in information oriented age, are expected.