축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

알레르기성 비염 환자에서 절지동물성 특이항원의 양성율 및 항원간 일치율

황규윤,남해선,박준수,권준택,백병준,김장묵,오천환,조성란 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

The current study was designed to evaluate the positive rates of arthropod allergens by Korean inhalant panel of MAST-CLA in patients with allergic rhinitis. One hundred sixty patients, diagnosed to allergic rhinitis in an Out Patient Center of Soonchunhyang University Chunan Hopsital during Aug 1998 to July 2000, were studied. 1. The overall positive rate among 35 specific allergens was 59.4% (95 patients). 2. The arthropod related allergen accounted for 82.1% (78 patients) of the positive rate. 3. Each positive rate of Dermatophagoides farinae (Df), Dermatophagoides pteronyssinus (Dp), house dust (Hd), and cockroach mix (Cm) was 45.0% (72 patients), 43.1% (69 patients), 28.1% (45 patients), and 8.8% (14 patients), respectively. 4.Significant agreement between arthropod allergens was observer (P < 0.05). 5. The obseved agreement between Df and Dp was 95.6%, showing most significant Kappa index (Kappa = 0.911, P < 0.001). There results suggested that although MAST-CLA is a useful measure of identifying specific allergens, the specificity of two mite allergen relatively low, implying because of common allergen between mites in Korean panel of MAST-CLA. Our data also supported that arthropod is the major cause of allergen in patients with allergic rhinitis, however, the MAST-CLA should be modified in order to exclude common allergen of mite and to include more specific mite allergen.

알레르기 환자에서 혈청 IgE와 특이항원 양성율의 일치도

남해선,권준택,박준수 순천향의학연구소 2000 Journal of Soonchunhyang Medical Science Vol.6 No.2

To evaluate usefulness of serum IgE to determine specific allergens, authors conducted priest IgE and MAST-CLA allergen-specific IgE assays in 78 allergic patients diagnosed at Soonchunhyang University Chunan Hospital during October 1998 through July 2000. During the study period, inhalent and food Korean panels were applied in 57 (73.1%) and 21 outpatients (26.9%), respectively. Kappa index was calculated to evaluate the agreement of priest IgE with Total IgE and allergen-specific IgE by MAST-CLA. 1. The men (±SD) age was 13.2 (±14.1) year-old and male accounted for 62.8%. 2. The overall positive rate of priest IgE was 66.7% and mean (±SD) level of priest IgE was 271.2 (±265.6) IU/ml 3. Patients with more than one specific allergen and with elevated total IgE by MAST-CLA were 25 (32.1%) and 33 (42.3%), respectively. 4. Significant agreement between total IgE and allergen-specific IgE was shown, but strength of agreement was slight (Kappa = 0.186, P = 0.046), 5. While no significant agreement between priest IgE and total IgE was observer, the priest IgE agreed significantly with allergen-specific IgE (Kappa = 0.153, P=0.043) In conclusion, our data suggested that serum priest IgE is more likely associated with allergen-specific IgE than total IgE in MAST-CLA assay. Furthermore, it is recommended that Korean panel of MAST-CLA should contain more specific allergens.

알코올성 췌장염 환자에서 CYP2E1 유전자형의 다형성

김일환,염윤기,채석,권준택,김형기,박상흠 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Cytochrome P4502E1(CYP2E1) is inducible by ethyl alcohol and activates procarcinogenic N-nitrosodimethylamine(NDMA), benzene, urethane and other lower molecular weight compound. CYP2E1 is also involved in metabolism of certain drugs, for example, alcohol, acetaminophen, tamoxifen, theophylline, flouorinated anesthetics, and chlorozoxazone. CYP2E1 activity was shown to be polymorphically distributed in humans and has been suggested to play a role in hepatocellular carcinoma and alcoholic related disorders. Although genetic predispodition to alcoholism and alcoholic liver disease has been reported, genetic susceptibility to alcoholic pancreatitis is still a matter of debate. The aims of this study were to investigate the allelic frequency of CYP2E1 Rsa I polymorphism in Koreans with alcoholic pancreatitis patients. We investigated the frequency distribution of CYP2E1 Rsa I polymorphism in 212 unrelated healthy Koreans and 64 alcoholic pancreatitis patients. Detection of the CYP2E1 alleles was performed by polymerase chain reaction and restriction fragment length analysis. The genomic DNA was isolated from peripheral blood with conventional phenol: chloroforn extraction method. The allelic frequencies of c1 and c2 in healty volunteers were 0.85 and 0.15, respectively. The allelic frequencies of c1 and c2 in alcoholic pancreatitis patients were 0.8 and 0.2, respectively. The rare type (c2/c2) of CYP2E1 Rsa I polymorphism was 0.005 and 0.031 (p<0.001) in healthy volunteers and alcoholic pancreatitis patients, respectively. However, the CYP2E1 genotype showed no significant relation to the alcoholic pancreatitis patients. It was suggested that the risk of alcoholic pancreatitis diagnosed clinically seem to be not associated with the CYP2E1 genotype.

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

Chung-Tack Han,Myoung-Jun Kim,Seol-Hee Moon,Yu-Rim Jeon,Jae-Sik Hwang,Chunja Nam,Chong-Woo Park,Sun-Ho Lee,Jae-Bum Na,Chan-Sung Park,Hee-Won Park,Jung-Min Lee,Ho-Song Jang,Sun-Hee Park,Kyoung-Goo Han 한국독성학회 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverseeffect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

Acute and 28-Day Subacute Toxicity Studies of Hexane Extracts of the Roots of Lithospermum erythrorhizon in Sprague-Dawley Rats

Han, Chung-Tack,Kim, Myoung-Jun,Moon, Seol-Hee,Jeon, Yu-Rim,Hwang, Jae-Sik,Nam, Chunja,Park, Chong-Woo,Lee, Sun-Ho,Na, Jae-Bum,Park, Chan-Sung,Park, Hee-Won,Lee, Jung-Min,Jang, Ho-Song,Park, Sun-Hee,H Korean Society of ToxicologyKorea Environmental Mu 2015 Toxicological Research Vol.31 No.4

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

염색가공공장 폐수로부터 폐열회수를 위한 열펌프 기술의 적용성 검토

박영태(Young-Tae Park),함성원(Sung-Won Ham),허만우(Man-Woo Huh),공성용(Sung-Yong Gong),장기창(Ki-Chang Chang),박성룡(Seong-Ryong Park),박준택(Jun-Tack Park) 한국염색가공학회 2001 韓國染色加工學會誌 Vol.13 No.3

보행속력과 동작의 부드러움과의 상관관계에 관한 연구

탁계래,한영민,최진승,이정한,임영태,전재훈,박상균,박승하,Stefanyshin Darren 한국운동역학회 2006 한국운동역학회지 Vol.16 No.1

적혈구 탐식소견을 보인 원발성 형질세포성 백혈병 1 예

박경현,이종명,박동철,이진관,이승표,정세주,홍영애,허충,박후군,조준탁 대한내과학회 1989 대한내과학회지 Vol.37 No.2

Primary plasma cell leukemia (PCL) is a very rare form of plasma cell dyscrasia. Phagocytosis of cellular elements by malignant cells is generally considered to be a feature of monocytic-histiocytic disorders, althogh it has been reported to occur rarely in other forms of neoplasia. The patient was an 18-year old male who presented weadness, fever, nasal bleeding and hepatosplenomegaly of 1.5 months duration. Peripheral blood examination revealed severe anemia, thrombocytopenia and a normal leukocyte count with 32% plasma cells. On serum and urine protein electrophoresis, monoclonal gammopathy was found. The analysis of the abnormal gamma-globulin showed IgA and kappa type on immunoelectrophoresis. A bone marrow smear from the right anterior superior iliac spine disclosed hypercellularity with 66.1% plasma cells (48.7% immature type), Some of the plasma cells showed erythrophagocytosis. The number of other cellular elements in marrow was decreased. A partial remission was obtained through the following treatment with a combination of vincristine, cyclophosphamide, Mephalan and prednisolone, But increased plasma cells in peripheral blood, anemia and development of extramedullary plasmacytoma appeared 6 months later. We thought that this case fulfilled the primary PCL criteria.

담관암을 동반한 Caroli 병 1 예

박경현,이은경,박동철,김용림,이진관,허충,박후근,정세주,조준탁,이수기,김규란 대한내과학회 1991 대한내과학회지 Vol.39 No.6

Caroli's disease is a rare congenital disorder characterized by segmental saccular dilatation of the intrahepatic bile ducts, but the mode of it's inheritance is not clarified. It is commonly complicated with cholangitis, cholelithiasis, hepatic abscess, septicemia and is frequently associated with other congenital disorders, such as congenital hepatic fibrosis, choledochal cyst, medullary sponge kidney, etc. Some authors regard this disease as a premalignant condition because of the high incidence (7%) of the bile duct carcinoma. Recently, we experienced a case of Caroli's disease complicated with cholangitis, cholelithiasis, clonorchiasis, cholangiocarcinoma in the dilated intrahepatic duct and adenocarcinoma in the common hepatic duct. Diagnosis was made by abdominal ultrasonography, percutaneous transhepatic cholangiography, abdominal computed tomography and intraoperative wedge biopsy of the liver. Roux-en-Y hepaticojejunostomy after segmental resection of the common hepatic duct and cholecystectomy was performd. Our report describes Caroli's disease associated with bile duct carcinoma and reviews the literature on the disorder.