효소 단백 가수분해물의 항균 활성

주정현,이상덕,이정옥,오만진,이기춘 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

밀 단백질에 효소가수분해 할 때 생산되는 peptide의 항균활성과 천연항균제로서의 이용가능성을 검토하기 위하여 실험을 행하였다. 밀 단백질에 7종의 단백질가수분해효소를 작용시켜 생성된 가수분해물의 항균활성을 측정하고 한외여과, membrane filtration, HPLC를 이용하여 항균성peptide를 분리 정제한 후 분자량과 아미노산 결합 순서를 측정한 결과는 다음과 같다. 밀 단백질에 7종의 단백질 분해효소를 적용시켜 제조한 가수분해물중 Asp. saito protease를 적용시켜 얻어진 peptide 만이 항균활성을 나타내었다. Asp. saito protease는 37 ℃, pH 6.0에서 작용시킨 경우에 항균활성이 가장 높았으며, 50℃ 이상에서는 활성을 나타내지 않았다. 밀단백 효소가수분해물은 membrane filtration 에 의하여 분자량 1.000~3.000에서 항균활성이 나타났다. Membrane filtration으로 얻어진 항균활성분획을 HPLC로 분리한 결과 retention time 31.1~31.8 min에서 항균활성을 나타내었다. 밀단백 효소가수분해물은 121℃에서 15분간 가열하여도 효소활성이 유지되는 매우 안정한 화합물이었다. 항균활성분획을 MALDI-mass로 질량을 분석한 결과 1.633이었다. 항균성 peptide의 아미노산 결합순서는 cysteine, glycine, prolin, prolin, polin, valine, valine, alanine, alanine, arginine의 순서였다. This study was carried out to investigate whether peptide produced from wheat protein by enzyme hydrolysis can be used as a natural antimicrobial agent. Antimicrobial peptide was obtained form wheat protein by protease of 7 species. The produced antimicrobial peptide was purified through ultrafiltration membrane filtration and HPLC, and molecular weight and amino and sequence of the purified antimicrobial peptide were determined. Among hydrolysate produced from wheat protein by protease of 7 species, antimicrobial activity was observed for the peptide obtained from Asp. satio protease. The Asp. satio protease did production antimicrobial hydrolysate showing the highest antimicrobial activity at reaction condition of 37℃ and pH 6.0, but not at reaction condition above 50℃. Wheat protein hydrolysate was fractionated by membrane filtration and showed antimicrobial activity between molecular weight 1,000 - 3,000. The antimicrobial activity fraction obtained by membrane filtration was separated through HPLC and showed antimicrobial activity in the peak of retention time 31.1 - 31.8 min. Since after wheat protein protease hydrolysate was heated during 15 min at 121℃, antimicrobial activity was maintained we could be conviction as heat-stable peptide. Molecular weight of antimicrobial peptide identified by MALDI-mass was 1,633. Amino acid sequence of antimicrobial peptide was cysteine, glycine, prolin, prolin, prolin, valine, valine, alanine, alanine and arginine.

NeX-Pert를 활용한 프로젝트 관리에 관한 연구

오기환,정한일,엄주태 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.2

In recent, as the application domain of information system becomes large, the project management concept is applied to not only the large-scale construction projects but also the development of an information system. Most of the ERP vendors release methodologies and software for project management. The success stories of applying the project management concept to the large-scale construction projects and development of an information system have been reported. However a case study for applying the project management technology to small-scale projects was not reported yet. This paper is intended to show the case study for applying the project management technology to small-scale project using the project management software, NeX-Pert.

양극성 반고관절 성형술을 이용한 대퇴 경부 골절의 치료

오정환,조병주,안의환 건국대학교 의과학연구소 1998 건국의과학학술지 Vol.8 No.-

Problems of nonunion, avascular necrosis of the femoral head, and loss of fixation are frequently encountered in the management of femoral neck fractures and high complication rates after closed or open reduction and internal fixation technique were reported due to poor bone quality or lack of cooperation in elderly patients. So, another option of hemiarthroplasty for the management of the femoral neck fractures were recommended, especially in elderly patients. We reviewed 20 bipolar hemiarthroplasties which had been performed from April, 1990 to March, 1996 for the femoral neck fractures and followed up for a minimum of 1 year and are reporting their results.

일회통과 관류실험시 물의 수송 : 관류액의 종류와 삼투압의 영향 Effects of Some Perfusates and Their Osmolality

이정화,이현주,용철순,오두만 영남대학교 약품개발연구소 1995 영남대학교 약품개발연구소 연구업적집 Vol.5 No.-

The single pass perfusion experiments were performed in anesthetized rata to investigate the effects of perfusates and their osmolality on the water transport and to determine the correlation between the extent of water transport and the volume change of perfusate. Phenol red was used as a nonabsorbable marker. In normal rats, when perfused at a flow rate of 0.5 ml/min 2-(N-morpholino) ethanesulfonic acid (MES) aid Sorensen's phosphate hullers showed minimal net water transport as 0.125 and 0.173 %/cm of intestinal length, respecetively. Hypotonic perfusate of 200 mOsm㎏ of water and hypertonic perfusate of 400 mOsm/㎏ of water generated significant water transport compared with isotonic perfusate of 300 mOsn/㎏ of water. There was a linear correlation between the attent of water transport and the volume change of perfusate, suggesting that the volume change can be used as a measure of water transport.

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

열화된 SUS 316강의 시간-주파수 해석에 의한 비파괴평가

이주석,오정환,이건찬,남기우 韓國非破壞檢査學會 1999 한국비파괴검사학회지 Vol.19 No.4

지금까지 퓨리에 변환이 신호 처리법으로 가장 널리 사용되고 있다. 그러나, 이 방법은 신호의 주파수 성분이 시간에 대하여 어떻게 변화하는지를 표현하지 못한다. 따라서, 최근 이와같이 비정상신호를 표현하지 못하는 퓨리에 변환의 단점을 보완하여, 신호의 시간과 주파수에 대한 정보를 동시에 표현할 수 있는 시간-주파수 해석법들이 개발되기 시작하였다. 본 연구에서는 인공열화된 SUS 316강의 초음파 신호를 시간-주파수 해석법으로 분석하였다. 특히, 단시간 퓨리에 변화법과 위그너 빌 분포법을 이용하여 초음파 신호의 주파수 성분과 특성을 분석하였다. Fourier transform has been one of the most commonly used tools in study of frequency characteristics of signal. However, based on the Fourier transform, it is hard to tell whether a signal's frequency contents evolve in time or not. Recently, to overcome Fourier transform fault, not to represent non-stationary signal, time-frequency analysis methods are developed and those can represent informations of signal's time and frequency at the same time. In this study, we analysed ultrasonic signal for degraded SUS 316 with time-frequency analysis method. In particular, the methods such as short time Fourier(STFT) and Wigner-Ville distribution(WVD) were used to extract frequency contents and characteristics from ultrasonic signals.

육백산(강원도, 삼척시)의 거미상

김주필(Joo Pil Kim),예용택(Yong Taek Ye),오종화(Jong Hwa Oh),예성훈(Seong Hun Ye),성민규(Min Gyu Seong),이정준(Jeong Jun Lee),김태우(Tae Woo Kim),김대희(Dae Hee Kim),지승환(Seung Hwan Ji),이형민(Hyeong Min Lee) 한국거미연구소 2016 한국거미 Vol.32 No.1

2015년 8월 3일부터 5일까지 한국거미연구회 회원들은 강원도 삼척시 육백산을 채집 조사한 결과 22과 49속 63종이 채집 조사되어 이에 보고하는 바이다. The authors investigated the spiders of the Mt. Yukbaek from August 3th to August 5th, 2015. In the present paper 63 species of 49 genera belong to 22 families were identified. These specimens are deposited at the Joo-Pil Spider Museum, Namyangju-Si, Gyeonggi-Do.

세가지 니켈 티타늄 파일의 휨과 비틀림 조건에서의 응력 분포에 관한 3차원 유한요소 연구

김태오,이찬주,김병민,박정길,허복,김현철 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.4

이 연구의 목적은 세가지 니켈-티타늄 파일의 휨과 회전 조건 하에서의 응력 분포를 유한요소 모형을 이용하여 비교하는 것이다. ProFile .06/#30, ProTaper와 ProTaper Universal의 F3파일을 마이크로컴퓨터 단층촬영을 하고 reverse engineering을 통하여 세 니켈 티타늄 파일의 구조를 얻고 삼차원 유한요소모형을 제작하였다. 니켈 티타늄 합금의 비선형적인 물리적 성질을 반영하고 ABAQUS 프로그램을 이용하여 휨과 회전 조건 하에서의 기계적인 움직임을 수학적으로 예측 분석하였다. U-형태의 단면 구조를 가진 ProFile이 모형 가운데 가장 좋은 휨 성질을 나타냈다. 동일한 휨량 조건에서는 볼록한 삼각형 단면의 ProTaper가 다른모형보다 많은 힘을 필요로 하였으며, 반면에 가장 높은 von Mises 응력은 ProTaper Universal의 단면에서 움푹 파인 부위에 집중되었다. ProFile 모형은 동일한 크기의 회전력에 대해 가장 큰 응력 집중을 U-형 구 부위에 나타냈다. ProTaper 모형은 다른 모형에 비해 동일량을 비틀기 위해 더 많은 힘을 필요로 하였으며, 반면에 동량의 비틀림에서는 가장 높은 von Mises 응력이 ProTaper Universal의 단면에서 움푹 파인 부위에 집중되었다. Flexibility and fracture properties determine the performance of NiTi rotary instruments. The purpose of this study was to evaluate how geometrical differences between three NiTi instruments affect the deformation and stress distributions under bending and torsional conditions using finite element analysis. Three NiTi files (ProFile .06 / #30, F3 of ProTaper and ProTaper Universal) were scanned using a Micro-CT. The obtained structural geometries were meshed with linear, eight-noded hexahedral elements. The mechanical behavior (deformation and von Mises equivalent stress) of the three endodontic instruments were analyzed under four bending and rotational conditions using ABAQUS finite element analysis software. The nonlinear mechanical behavior of the NiTi was taken into account. The U-shaped cross sectional geometry of ProFile showed the highest flexibility of the three file models. The ProTaper, which has a convex triangular cross-section, was the most stiff file model. For the same deflection, the ProTaper required more force to reach the same deflection as the other models, and needed more torque than other models for the same amount of rotation. The highest von Mises stress value was found at the groove area in the cross-section of the ProTaper Universal. Under torsion, all files showed highest stresses at their groove area. The ProFile showed highest von Mises stress value under the same torsional moment while the ProTaper Universal showed the highest value under same rotational angle.

工業敎育大學 敎育改善에 관한 硏究(Ⅲ)

宋周鎬,李昌岬,洪元杓,孟琦錫,任洪彬,李廷玟,李載元,李來烈,權五憲,金振淳,李文洙 충남대학교 공업교육연구소 1982 論文集 Vol.5 No.1

This paper aims at providing a concrete plan to improve technical and vocational teacher education in the College of Industrial Education of Chungnam National University. It is intended to propose a practical four-year regular and short-term course. Also, the estimated required facilities is included in this study as well as their management. The main conclusions and recommendations are summarized as follows; 1) The numbers of students enrollment should be reduced to 220 students which is the approximate number of future demand. 2) In order to offer to the students a broader education and to cope with the increase/decrease in demand of technical and vocational teacher within a specific field, the 8 department existing at the present (Department of Architecture Engineering Education, Civil Eng. Educ., Metallurgical Eng. Educ., Mechanical Eng. .Educ., Electrical Eng. Educ., Electronic Eng. Educ., Industrial Chemistry Educ. and Industrial Technology Educ.) would be combined into 5 (Department of Construction Engineering Education, Mechanical Eng. Educ., Electrical Eng. Educ., Industrial Chemistry Educ. and Industrial Technology Educ.). 3) The short-term course program is recommened to provide additional training and education to those candidates who already have experience in technical fields to meet an occasional demand. 4) It is necessary to add the Teaching Profession Section in the administrative organization of the College.

접속방법에 따른 접지도체 굵기 제안식

崔洪圭,吳廷錫,宋榮朱 弘益大學校 産業技術硏究所 2004 産業技術 Vol.14 No.-

접지도체의 굵기를 결정하는 국외 규격은 재료의 특성값인 물리적 정수까지 적용하여 광범위하게 적용할 수 있는 반면 수식이 복잡한 문제점이 있고 JIS와 국내규격은 수식은 간단하나 제약조건이 많은 문제점이 있다. 또한, 접지도체의 접속방법에 따라 최대허용온도가 다르게 적용되므로 접지도체 굵기 산정 방식이 복잡하고 적용 방법이 상이하여 시공현장에서 엔지니어들이 적용하는데 많은 어려움이 있다. 따라서, 본 논문에서는 새로운 접지도체 굵기 산정방식을 제시하고 접속방법에 따른 접지도체 굵기 산정 간이식을 제시한다.