Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

정상면역을 가진 성인에서 발생한 식도 방선균증

김현수 ( Hyun Soo Kim ),천종운 ( Jong Woon Cheon ),김민수 ( Min Su Kim ),정창길 ( Chang Kil Jung ),김경록 ( Kyung Rok Kim ),최재원 ( Jae Won Choi ),강동우 ( Dong Woo Kang ),김선영 ( Sun Young Kim ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Hyun Soo Kim, Jong Woon Cheon, Min Su Kim, Chang Kil Jung, Kyung Rok Kim, Jae Won Choi, Dong Woo Kang and Sun Young Kim1 Departments of Internal Medicine and Pathology1, DongKang Medical Center, Ulsan, Korea Actinomycosis is a chronic suppurative disease and caused by Actinomycosis species, principally Actinomyces israelii, which are part of the normal inhabitant on the mucous membrane of the oropharynx, gastrointestinal tract, and urogenital tract. It usually affects cervicofacial, thoracic and abdominal tissue. Cervicofacial type has the highest percentage of occurrence with 50%. Actinomycosis frequently occurs following dental extraction, jaw surgery, chronic infection or poor oral hygiene. It may also be considered as an opportunistic infection in immunocompromised patients such as malignancy, human immunodeficiency virus infection, diabetes mellitus, steroid usage or alcoholism. But, actinomycosis rarely occurs in adults with normal immunity and rare in the esophagus. We report an unusual case of esophageal actinomycosis which was developed in a patient with normal immunity and improved by therapy with intravenous penicillin G followed oral amoxicillin, and we also reviewed the associated literature.

Promotional Strategies of Local Drugstores

Kim,Seung-Mi,Lee,Sang-Yoon,Kim,Pan-Jin,Kim,Nam-Myun,Youn,Myoung-Kil 한국유통과학회 1999 유통과학연구 Vol.1 No.1

The retail business of drugstore was introduced to Korea for the first time 10 years ago. Since Olive Young introduced a retail store in the name of drugstore in 1999 for the first time in Korea, new distribution channel combining drugstore, cosmetic products and dairy products, etc has been made. At initial stage, the new distribution channel grew up slowly because of low specialty and economic stagnation. However, the three big distribution channels, that is to say, Olive Young (CJ), Watsons (GS) and W Store (Kolon Well Care), etc, were established to produce new distribution system following large-scaled discount stores as well as convenience stores. The purpose of the study is to investigate ways making Korean style drugstore be new retail business in addition to traditional markets, department stores, E-Mart and other general super markets and to examine problems preventing the drugstore from being promoted and to find out solutions. The speciality retailers that is called a category killer attacking department stores as well as marts is expanding market quickly. New consumption trend that gives priority to wellbeing is being expanded in accordance with high level of standards of living life: The drugstore is thought to be new alternative of distribution because it keeps special products. Young ladies who are main customers of drugstores respond to the trend sensitively to have more buying power that is thought to be promising. And, consumers' desire has become concrete and special. This is because consumers want not only convenient shopping but also special shopping system that is current trend. These days, so called Multi-shop and Total shop and other special shops have been recently opened. Special multi-shop has been concentrated on fashion product and miscellaneous goods so far: Health total wellbeing shop shall be popular in accordance with wellbeing trends. Drugstores can play an important role. Drugstores were opened for the first time ten years ago. In

QT연장 증후군 환자에서 Head-Up Tilt Test에 의해 유발된 Torsades De Pointes 1례

전익수,차태준,김길수,김동완,김규종,김찬욱,김성만,주승재,이재우 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8

제외 의미의 5가지 'but+전치사' 구조 유형 비교

안병길(Ahn, Byeong-kil),김두식(Doo-Shick Kim) 경희대학교 언어정보연구소 2013 언어연구 Vol.30 No.2

There are a few expressions which mean "exception" in English; among which are 'but' and 'but for.' The but-structures have been analyzed as so-called 'but-cleft' by Kim(2005), where Kim proposed that <X but Y> correlatives are required to satisfy the 3 constraints on their occurrences: the endorsement(E) condition, the semantic condition(S) of 'part-whole'(S) and a structural or functional parallelism(P) condition between X and Y. This study has dual goal: 1) to show that a slightly revised version of the E/S/P-Conditions by Kim's (2005) for <X but Y> construction can be applied for the distinction in use between <X but Y> construction and its alternative form <X but + preposition Y> and 2) to identify five different types of <X but + preposition Y> constructions in terms of the optionality/kinds/grammatical statuses of an occurring preposition, the meanings/grammatical statuses of 'but' and the exclusive uses for the <X but + preposition Y> structures of 'revised' E/S/P-Conditions.

기종성 방광염을 동반한 기종성 신우신염 1예

김선두,김순제,이길도,김정수,김지훈,윤호상,송종오,문언수 대한노인병학회 2000 Annals of geriatric medicine and research Vol.4 No.3

CURRENT CONVEYOR에 의한 전류측정 프로우브 및 비접지 인닥턴스의 실현

金明起,金斗泳,金吉重 동아대학교 공과대학 부설 한국자원개발연구소 1980 硏究報告 Vol.4 No.2

Current conveyors as relatively newer active building blocks have been introduced by Smith and Sedra, and many possible applications have been explored during the past few years. The operation of the current conveyors is based on the principle of conveying equal currents at its output and input ports at different admittance levels. In this paper, a second generation current conveyor (CC-Ⅱ) with negative sign is applied to the design of a simple current measuring probe and to the synthesis of the floating inductance. Experiments were performed by using the CC-Ⅱ consisting of operational amplifiers realized by M. Sharif Bakhtiar and P. Aronhime. Experimental results agreed well with the theoretical values, and confirmed that the frequency limitation was confined by the frequency response of CC.-Ⅱ. If operational amplifiers with high frequency capability were used to realize the CC-Ⅱ, the frequency limitation could be improved.

Mirizzi 증후군의 변형된 분류와 치료

김형철,강길호,채만규,김성용,백무준,이문수,박상흠,이문호,김창호,송옥평,조무식,박희주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Purpose : The Mirizzi syndrome is relatively rare and preoperative diagnosis of this disease is difficult. In 1978, Morelli suggested the subclassification of the Mirizzi syndrome into acute or chronic form. We experienced 5 cases of acute form. We analysed clinical features, preoperative radiologic findings and operative findings of 18 cases including acute forms which were diagnosed as Mirizzi syndrome and should suggest the modified classification of Mirizzi syndrome for choice of appropriate treatment. Method : From January 1995 to December 1998, 18 cases, of which 8 cases were diagnosed at Soonchunhyang University Chunan Hospital, and 10 cases were reported in the Korean Journal were retrospectively analysed with regard to clinical features, preoperative radiologic findings and operative findings. According to the clinical features, whole cases were divided into type Ⅰ(acute form) and type Ⅱ(chronic form) and then each type of cases were subclassified according to preoperative radiologic findings and operative findings. Results : Of 18 cases there were 5 cases in type Ⅰ(27.8%), 13 cases in type Ⅱ(72.2%). Type Ⅱb was most common. Type Ⅰa cases were treated only with cholecystectomy. We applied cholecystectomy, T-tube choledochostomy and patch technique in type Ⅰb and thpe Ⅰc cases. Cholectystectomies including removal of gallstones and internal drainage procedures were done in type Ⅱ chronic forms. Conclusion : The acute form(Type Ⅰ) of Mirizzi syndrome was suggested by Morelli might be subclassified into typeⅠa,Ⅰb and Ⅰc following the presence of the necrotic defect in common hepatic duct. Through the modified classification of Mirizzi syndrome based on clinical feature, preoperative radiologic findings and operative findings, we can choice appropriate treatment.

폐결핵으로 오인된 SIADH를 동반한 편평상피 세포폐암 1예

김순제,류하근,김선두,이길도,박봉안,류주성,유재등,김인숙,신현준,문언수 대한노인병학회 1999 Annals of geriatric medicine and research Vol.3 No.4

山野草의 園藝化에 관한 硏究

김정길 群山大學校 自然科學硏究所 1991 自然科學硏究 Vol.6 No.-

山野草의 園藝化 可能性을 살피기 위하여 우리의 생활주변에 있는 野生草를 採集하여 分布, 生態, 栽培, 利用方法을 調査한 바를 要約하면 다음과 같다. 1. 엉겅퀴(Cirsium japonicum var. ussuriense ??.) 국화과 採集은 군산시 米龍山. 多年生草本으로 6-8월에 淡紫色의 꽃이 핀다. 性質이 强健하여 재배하기 쉽고 分株實生으로 번식하며 盆植, 花壇植栽, 生花에도 쓰인다. 또 어린순과 뿌리를 食用으로 한다. 藥用으로는 吐血, 血便, 鼻血, 外傷出血에 全草를 건조하여 달여서 服用하고 外傷에는 씻기도 한다, 이 꽃은 유럽지방에서는 기독교의 聖花와 결혼성취의 꽃, 落雷를 막아주는 神의 꽃, 스코틀랜드王家의 紋章으로 하는등 民俗的 傳設이 있다. 이 꽃은 모양이 장엄한 인상적인 꽃으로서 C. japonicum Dc. 같은 원예품중이 있다. 앞으로 더 많은 種間雜種育成과 根再生法등의 硏究가 필요하다(Fig.1). 2. 떡쑥(Gnaphatium affine D.??), 국화과 採集은 군산시 미룡산 路邊. 2年生草로서 4-6월에 淡黃色의 꽃이 피는데 全草에 白色의 線毛가 密生한다. 햇빛이 있는 路邊에 雜草와 같이 生育하나 白色털이 많이 있어서 언듯 高山植物과 같이 보이며 나름대로 觀賞美가 있어 보인다. 實生繁殖은 어렵고 自生한 어린苗를 分에 植生하면 잘 자란다. 옛날에는 잎 줄기를 죽에 넣거나 떡을 만들어 먹었다고 하며 藥用으로는 全草를 開花期에 채취하여 건조한 것을 천식, 감기, 근육통에 달여서 服用한다. 또 印度에서는 收斂劑나 傷藥으로 사용한다. 앞으로 實生法과 揷木法의 硏究가 必要하다(Fig.2). 3. 이고들빼기(Youngia denticulata ??), 국화과 採集은 全南 突山島 丘陵地. 2年草로서 8-9월에 黃色꽃이 핀다. 어린잎은 가축의 먹이나 김치로 食用하기 때문에 種子를 파종하거나 自生의 幼苗를 移植한다. 藥用으로는 개화기에 꽃과 줄기를 채취하여 말린 것을 분말로 하여 腫氣가 생긴 곳에 참기름을 섞어 환부에 바른다. 잎과 줄기가 暗紫褐色을 띠고 있는 곳에 黃金色의 아름다운 꽃이 피게 되면 한층 더 돋보이기 때문에 花卉的 가치가 있어 보인다. 他種과 잘 交雜하는 性質이 있어 種間育種이 可能하다. 4. 타래난초(Spiranthes sinensis(??.) ??), 난초가 採集은 군산시 미룡산 묘지 잔디밭. 多年草로서 5-8월에 분홍색 꽃이 螺族狀으로 꼬인 穗狀花序로 밑에서 위로 피어 올라간다. 번식은 自生株 부근에서 幼苗를 캐어 移植하거나 種子를 水苔床에 取播한다. 꽃이 나사모양으로 꼬여가며 피는 것이 흥미로우나 줄기가 가늘고 잎이 작기 때문에 많은 個體를 集團으로 심는 것이 운치가 있어서 좋다. 生態的 育種開發이 必要하다.(Fig. 4). 5. 백선(Dictamus dasycarpus ??), 운향과 採集은 군산시 미룡산 南面 低地. 多年草이며 높이 1m, 全體에 강한 香氣가 풍긴다. 꽃은 5-6월에 白色 또는 淡紫色으로 총상꽃차례로 핀다. 재배는 陽地의 石灰質을 함유한 砂壤土가 적당하며 번식은 種子파종한다. 개화기까지는 4-5年 걸리기 때문에 더 빠른 증식은 뿌리를 揷木한다. 成分으로 Dictamine 등이 포함되었으며 藥用으로 黃疸, 解毒, 强心作用에 쓰인다. 漢方의 貴重한 藥劑로서 開發價値가 높으며 取木, 實生法의 硏究가 더 必要하다(Fig. 5). 6. 까치수영(Lysimachia barystachys ??), 앵초과 採集은 군산시 미룡산 路邊. 陽地에서 보통 볼 수 있는 키가 높은 多年草이며 6-8월에 줄기끝에 많은 白色 꽃이 總狀으로 密着하고 花序는 上部가 한쪽으로 기울고 길이 10-30cm이다. 耐冬性이며 適濕한 壤土에 잘 生育하므로 花壇植에 좋고 번식은 春秋에 分株나 實生으로 한다. 원예종으로는 L. fortunei와 L. barystachys와의 交雜種이 있다. 成分은 Saponin과 Primverase가 함유되었으며 어린순을 삶아서 水洗後 食用으로 한다. 또 全草를 利尿와 月經不調등의 藥으로 쓰인다. 花卉으로나 食用과 藥用에 有用하므로 種間雜種育成, 分株, 種子繁殖등의 硏究가 더욱 必要하다(Fig.6). 7. 돌나물Sedum sarmentosum ??), 돌나물과 採集은 군산시 팔마산 기슭. 多年草이며 약간 습기가 있는 곳에서 자라며 가지가 갈라져서 地面을 벋고 뿌리가 내린다. 잎은 3개식 輪生하고 5-6월 별모양의 黃色 꽃이 핀다. 山草와 多肉植物의 中間的 存在이며 花壇·岩壁植栽, 盆植, 切花등으로 쓰인다. 排水가 좋고 腐葉土가 많은 輕土가 적당하다. Glycoside등의 成分이 있고 채소로 김치를 만들어 먹거나 藥用으로는 帶下症, 鮮血劑, 인후통, 肝炎, 뱀과 벌레 물린데 쓰인다. 不稔性으로 揷木으로 번식하며 앞으로 種間雜種育成, 葉揷등에 관한 硏究가 必要하다.(Fig. 7). 8. 창질경이(Plantago lanceolata L.), 질경이과 採集은 군산시 미룡산 路邊. 多年草로서 根莖은 굵고 肉質이며 葉은 모두 根出葉이고 披針形이다. 5-8월 穗狀花序로 白色 꽃이 핀다. 葉種은 유럽 原産인데 歸化하였으며 民間藥으로 쓰였던 民俗的 傳承의 話題가 많다. 또 自生種으로는 P. asiatica種이 있다. 成分은 씨에 plantasan과 葉에 aucbin등이 함유하고 利尿와 去痰劑로 사용한다. 陽地 長日性 植物이며 꽃대가 훤칠하니 높아 高潔美를 풍김으로 花卉化함도 좋게 생각되며 種子栽培法을 더 硏究하였으면 한다(Fig. 8 1-2). 9. 패랭이꽃(Dianthus sinensis L.), 석죽과 採集은 군산시 미룡산 低地. 多年草로서 줄기 높이 30cm이며 全草에 粉白色을 띤다. 잎은 對生으로 線形이다. 6-8월에 갈라진 줄기끝에 淡黃色의 꽃이 1개씩 핀다. 재배하고 있는 品種은 變種으로서 꽃잎이 系狀으로 細裂한 D. sinensis var. laciniata와 査季味性인 D. sinensis var. semperflorens, 꽃잎의 끝이 길고, 깊게 갈라진 D. superbus var. longcalycinus 및 D. barbatus var. japonicus 등이 있다. 種子를 봄파종 하면 다음해 8월에 꽃이 피고 가을 파종 하면 다음해 5월에 꽃이 핀다. 揷木과 取木은 3周內에 發根하며 6월이 適期이고 8월에 하면 겨울에 개화한다. 화단, 盆植, 生花로 觀賞하는데 敍情的인 분위기를 나아낸다. 陽地 中日性 植物이며 種間雜種育成이 더욱 有望하다(Fig. 9). 10. 꿀풀(Prunella vulgares var. lilacina ??), 꿀풀과 採集은 군산시 미룡산 산록. 多年草로서 높이 20cm정도이고 잎은 對生이며 길둥근 모양으로 톱니가 있다. 全草에 白色 털이 있다. 根部는 地下莖이 벋어 새순이 나온다. 그러나 變種인 var. aleutica는 地下莖이 없고 꽃이삭이 작다. 別種인 P. prunelliformis는 초장이 낮고 꽃이 크기 때문에 관상가치가 높아서 옛날부터 培養되고 있다. 藥用은 口內炎, 扁桃炎, 結膜炎 등에 使用되고 있다. 꿀풀은 芳響이 있어 密源으로 愛用되고 있기도 하며 앞으로 實生, 分株, 揷木, 種間·生態育種의 硏究도 바람직할 것이다(Fig. 10). This study, based on observation on distribution ecology, cultivation, and utilization of wild flowers, is a diagnosis of their horticultural possibilities. 1. Cirsium japonicum var. ussuriense ??. Station : Mt. Mi-ryong, Gun-san. This perennial herb, with light purple flowers from June through Ausust, is easy to cultivate by virtue of its strong properties. Propagation by ramification and seeding. Ornamental use: in Pots or in a flower bed, also for flower arrangement. Immature sprouts and roots are edible. Medicinal use : blood vomitting, bloody excrement, noseblood, and external wound cure. Juice from dried whole body is applied to each case above mentioned. The flower is recognized as a sacred one of Christanity in Europe, a matchmaking one, the God's flower protecting from thunderstroke, an emblem of the Scotish royal family. Besides, it has some legendary folk tales. There is a breed for horticulture, C. japonicum Dc., the flower of which has impressive looks of solemnity. More investigation on hybridization between species and root regrowth process is needed(fig. 1). 2. Gnaphatium affine D.D??. Station : the road side of Mt. Mi-ryong, Gun-san. A biennial grass with light yellow flowers from april through June. It is usually found on road sides in the sun. Its whole body is thickly covered with white pluffs, which make the herb look like an alpine plant and also add some ornamental beauty. Transplanting its wild young grass into a pot is favourable, while seeding has not been so successful. The leaves are said to have been used for food such as gruel and rice cake. Juice from dried plant collected during the flowering season is used for asthma, a cold, muscular pain cure and as cosmetics like an astringent. More investigations on seeding and cutting is needed(Fig. 2). 3. Youngia denticulata K??. Station: a low hill in Dol-san island, Chollanamdo. A biennial herb with yellow flowers from August through September. Young leaves are used as food for livestocks or Kim-chi. Seeding and transplanting are horticultural methods. Flowers and stalks picked during the flowering season, mixed with sesame oil, are applied to boils. In addition to dark purplish brown leaves and stalks, beautiful flowers of golden yellow color make its floricultural value even higher. Hybridization between species is possible by virtue of its well-crossing properties(Fig.3) 4. Spiranthes sinensis (??.) ??. Station: A grave yard lawn in Mt. Mi-ryong, Gun-san. A perennial herb with pink flowers from May through August. The flowers come out in a spiral form consisting of several spikes of flowers. Transplanting young sprouts of native wild grass of seeding on sphagnum bed are used for propagation. The flower attracts attention because of its getting twisted like a screw. Collective planting is good to see because the stalks thin and the leaves are small. Ecological breeding is recommended(Fig. 4). 5. Dictamus dasycarpus ??. Station: the southern low ground in Mt. Mi-ryong, Gun-san. This one-foot-high perennial herb sends out very sweet fragrance. It has white or light purple flower in a raceme from May through June. It grows well in sandy calcareous soil in sunny place. Seeding for propagation. It takes about four or five years for the flowers to come out, so root cutting is generally used for rapid propagation. Dictamine is a component of this herb. Medicinal use: jaundice, detoxification, a heart stimulant. This is a precious medicinal stuff worth developing its use. More investigation on cutting and seeding is needed(Fig. 5). 6. Lysimachia barystachys ??. Station: the road side in Mt. Mi-ryong Gun-san. A tall perennial herb usually found in a sunny place. From June through August white flowers come out and get dense in raceme form at the ends of the stalks. The inflorescence, whose upper part leans down, is ten to thirty centimeters long. This plant is suitable for garden planting because it has cole-bearing properties and grows well in rich soil of right humidity. Propagation by ramification or seeding in Spring or Fall. A species made by hybridization between L. fortunei and L. barystachys is used for horticulture. Saponin and Primverase are the components. The boiled sprouts are edible after being washed. The whole body is medicinally used for urination and menstrual irregularity, etc. This herb is used not only as a horticultural stuff but as food, medicine. Various methods of hybridization between species, ramification and seeding must be developed(Fig. 6). 7. Sedum sarmentosum ??. Station: the foot of Mt. Pal-ma, Gun-san. A perennial herb living in humid place. It stretches its split branches over the road and there come out new roots. the leaves verticillate one after the other. Star-shaped yellow flowers bloom from May through June. It is an intermediate being between wild herb and fleshy plants. Gardening, rock gardening, pot planting and cutting are used. Well-drained light soil with much leaf mold is suitable. Glycoside is one of the components. This grass is used for Kim-chi and for medicinal cure such as leucorrhoea, fresh blood making, a soar throat, inflammation, and snakes or insects bites. Only cutting is used because of its sterility. But more investigation on hybridiz ation between species and leaf cutting is needed(Fig. 7). 8. Plantago lanceolata L. Station: the road side of Mt. Mi-ryong, Gun-san. A perennial herb whose rootstock is thick and flesh, the leaves are radical and lanceolate. From May through August white flowers bloom in the form of a spike. This species, of which the origin is found in europe, was naturalized and used to be a home remedy for so long a time. Seeds have the component of plantasan, leaves of auchin. Medicinal use: urination and an expectorant. This herb is considered worth horticulturing because its tall and graceful body gives us noble feeling. Besides, it has a property of long day plant. More investigation on propagation by seeding is needed(Fig. 8-1, 2). 9.Dianthus sinensis L. Station ; low place of Mt. Mi-ryong, gun-san. A perennial herb which is about thirty centimeters high and white colored. Opposite leaves are linear. From June through August light purple flowers bloom at the end of each split branch. Several varieties are cultivated such as D. sinensis var. laciniata whose petals are subdivided like thin thread, the four season blooming D.sinensis var. semperflonens, D.superbus var. longcalycinus whose petals are long and deeply split and D. barbatus var. japonicus, etc. Seeds sowed in spring make flowers on August the next year, in Autumn on May the next year. In case of cutting and layering, roots come out within three weeks. June is right time for cutting and layering. If they are done in August, you can see flowers in Winter. Ornamental use: in flower bed or in pots, and for flower arrangement. This plant gives us some lyrical atmosphere. It is middle day plant living in a sunny place. Hybridization between species is a promising method(Fig. 9). 10. Prunella vulgaris var. lilacina ??. Station: Mt. Mi-ryong, Gun-san. A perennial herb which is about twenty centimeters high. The leaves are opposite and sawtoothed. It has white fluffs in its whole body. Subterranean stems of root grow and make new sprouts come out. But a variety, var. aleutica has not sub-stem and has small petals. P. prunelliformis, a different type of this species, is short and has big petals, so it has been cultivated from the past on. Medicinal use: stomatitis, tonsillitis, and conjunetivitis. This herb gives out fragrance and is favored as boney resources. More investigation on seeding, ramification, cutting, hybridization between species, ecological species breeding is needed(Fig. 10)