한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

전격성 제1형 당뇨병 1예

노동현,김도형,김나래,박종선,이창헌,김미경,최영식 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

전격성 제1형 당뇨병은 특발성 당뇨병환자에서 임상 경과가 짧고 진단 당시 심각한 대사 합병증을 동반하면서 췌장 효소 수치가 높고 췌도 특이 자가항체가 음성인 새로운 아형으로 알려져 있으며, 일본과 일본 외에서의 유병률에는 차이가 있는 것으로 보인다. 아직 전격성 제1형 당뇨병의 발생 기전은 명확하지 않으며 유전적 감수성이 있는 개체에서 바이러스 감염 등의 환경적인 인자와의 연관성이 가능한 병태생리로 제시되고 있으며 이에 대한 추가적인 연구가 요구된다. 저자들은 케톤산증으로 내원하여 전격성 제1형 당뇨병을 진단받은 증례를 경험하였기에 문헌고찰과 함께 보고 하는 바이다. Some patients with idiopathic type 1 diabetes have a fulminant disorder characterized by the absence of insulitis and of diabetes-related antibodies, a remarkably abrupt onset and high serum pancreatic enzyme concentrations. This is referred to as fulminant type 1 diabetes. A 29-year-old man suffering from abdominal pain, nausea and vomiting consulted to our hospital. Laboratory findings revealed high blood glucose level and the evidence of diabetic ketoacidosis, but the serum HbA1c was normal nevertheless. The low level of plasma C-peptide indicated the loss of endogenous insulin secretion. The patient satisfied the criteria for the diagnosis of fulminant type 1 diabetes. After the patient has been treated with insulin, his symptoms were improved and abnormal laboratory data were normalized. We report this case with a review of the literature

Prevalence of Clonorchis sinensis Metacercariae in Fish from Water Systems of Seomjin-gang (River)

Woon-Mok Sohn,Byoung-Kuk Na1,Shin-Hyeong Cho,Mi-Yeoun Park,Cheon-Hyeon Kim,Min-Ah Hwang,Kyeong-Woo No,Ki-Bok Yoon,Hyun-Cheol Lim 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.3

상부우장관 증사이 없는 한국인에서 Helicobacter pylori 감염의 혈청학적 유병률에 관한 전국적 역학조사

김나영(Na Young Kim),김재규(Jae Gyu Kim),김진호(Jin Ho Kim),김학양(Hak Yang Kim),김상우(Sang Woo Kim),김재준(Jae Jun Kim),노임환(Im Hwan Roe),심재건(Jae Geon Sim),안형식(Hyeong Sik Ahn),윤병철(Byung Chul Yoon),이상우(Sang Woo Lee),이용 대한내과학회 2000 대한내과학회지 Vol.59 No.4

Background : Helicobacter pylori infection occurs throughout the world and causes various gastroduodenal diseases in all age groups. The prevalence of H. pylori infection varies among countries and races. The aim of this study was to evaluate the seroprevalence of H. pylori infection in healthy people in Korea. Methods : From March 1998 to October 1998, 5,732 asymptomatic subjects responded to the self-assessment questionnaires from 54 hospitals were enrolled. Serum level of anti-H. pylori IgG was measured by ELISA test. Results : The overall prevalence of H. pylori infection was 46.6% and showed no significant difference between male (47.2%) and female (45.9%). According to the geographic areas, the highly prevalent provinces were Kangwon (53.4%), Cheju (52.9%) and Jeonra provinces (50.6%), while Seoul (41.9%) was the lowest prevalent area. The seroprevalence increased with age and was the highest at 40's (78.5%). The characteristic feature of this study was that the infection rate increased steeply in three age groups (10-12, 16-19 years old and 30's). In Seoul, there was no different prevalence rate among the districts studied.Conclusion : The nation-wide seroprevalence of H. pylori infection in Korea is higher than that of the developed countries. We hope that this study provides the landmark for the study of H. pylori infection in Korea.(Korean J Med 59:388-397, 2000)

무증상 한국인에서 Helicobacter Pylori 감염의 위험인자

김나영(Na Young Kim),김재규(Jae Gyu Kim),김진호(Jin Ho Kim),김학양(Hak Yang Kim),김상우(Sang Woo Kim),김재준(Jae Jun Kim),노임환(Im Hwan Roe),심재건(Jae Geon Sim),안형식(Hyeong Sik Ahn),윤병철(Byung Chul Yoon),이상우(Sang Woo Lee),이용 대한내과학회 2000 대한내과학회지 Vol.59 No.4

Background : The prevalence of Helicobacter pylori (H. pylori) infection varies between countries and between social classes. The aim of this study was to identify risk factors for with Helicobacter pylori infection in asymptomatic Korean population. Methods : Sera were collected from 2,687 females and 3,049 males (mean age, 29.1 y; range, 1 m-79 y) in Korea from Mar 1998 through Oct 1998. All asymptomatic subjects completed assessment questionnaires. An enzyme-linked immunosorbent assay was performed to detect IgG antibody to H. pylori.Results : The overall seroprevalence observed was 46.6% and showed no statistical diffrerence between female (45.9%) and male (47.2%). The seroprevalences in children (neonate-15 y) and adult (16-79 y) were 17.2% and 66.9%, respectively. According to multivariate analysis, variables such as sex, age, geographic area, crowding (number of person per room) in childhood, economic status in childhood, and types of housing in childhood were significantly and independently associated with H. pylori seroprevalence of adults. In children, age, geographic area, income, mother's education, and drinking water source were significant risk factors of H. pylori infection. Conclusion : Socioeconomic condition and close person to person contact in childhood are the significant determinants for H. pylori infection in adult. Drinking water source is an another important risk factor for H. pylori infection in children, suggesting the fecal to oral transmission in Korea.(Korean J Med 59:376-387, 2000)

간호대학생과 비 간호계열 대학생의 HIV/AIDS와 B형 간염에 대한 지식 및 태도

이형숙(Lee, Hyeong Suk),박해나(Park, Haena),김건희(Kim, Keun Hee),김미리(Kim, Mi Ri),김민지(Kim, Mingee),김빛나(Kim, Bit Na),김은경(Kim, Eun Kyung),김은영(Kim, Eun Yong),김진희(Kim, Jin Hee),성혜진(Seong, Hye Jin),송미선(Song, Misun) 한국보건간호학회 2015 韓國保健看護學會誌 Vol.29 No.3

Diphenylcyclopropenone 감작에 관한 연구

이국형(Kuk Hyeong Lee),김도원(Do Won Kim),나건연(Gun Yoen Na),김점용(Jum Young Kim),서기석(Ki Suk Suh) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.3

N/A A Study of Diphenylcyclopropenone Contact Senaitization Kuk Hyeong Lee, M.D., Do Won Kim, M.D., Gun Yoen Na, M.D.*, Jum Young Kim, M.D.,*, Ki Suk Suh, M.D.** Depertment of Dermatology, Kyungpook National University School of Medicine, Taegu, Korea Department of Dermatology, Pusan Armed Forces General Hospital, Pusan, Korea* Department of Dermatology, Kosin Medical College & Kosin Medical Center, Pusan, Korea** To facilitate measurement of cell mediated immune responses in the healthy adult, a method for quantitative clinical evaluation of contact sensitization to dipheny-lcyclopropenone(DPCP) was devised. Subjects consisted of 77 healthy men(21-24 years old) who were divided into four groups based on concentrations used in sensitization:4%(16 subjects), 2%(21 subject), 1%(20 subjects), and 0,5%(20 subject) DPCP. Each subject received a single 2cm topical application of 0.1ml of DPCP solution in acetone on the shoulder, which was washed it 48 hours later. Sensitization was expressed by the occurence of a sopntaneous flare-us(SFU) after eight to 16 days or a positive reponse on low concentration challenge on day 7, 14 and 21. The over-all rate of sensitization was 88.3%. Sensitization rate did not vary with concentration of DPCP(p>0.005). The average time for SFU occured was 9.9-12.2 days ; a positive response after low concentration challenge test occured on day 14. These results suggests that sensitization of DPCP can be achieved within 2 weeks by a single topical application of 0.1ml of 0.5% DPCP (Kor J Dermatol 29(3) : 292-297, 1991)

Dose-Response Effects of Epichlorohydrin on Male Reproductive Function in Rats

Kim, Kang-Hyeon,Shin, In-Sik,Lim, Jeong-Hyeon,Kim, Sung-Hwan,Park, Na-Hyeong,Moon, Chang-Jong,Kim, Sung-Ho,Shin, Dong-Ho,Kim, Jong-Choon Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.4

Present study was conducted to investigate potential effects of epichlorohydrin on testicular and epididymal function in male rats. The test chemical was administered to adult male rats by gavage at dose levels of 0, 3.125, 12.5, and 50 mg/kg/day for 7 days. Testicular and epididymal function were assessed by measurement of reproductive organ weight, testicular spermatid count, epididymal sperm count, motility and morphology, and histopathology in rats. At 50 mg/kg, a decrease in the sperm motility and an increase in the incidence of sperm abnormalities were observed. Histopathological examinations revealed an increase in the incidence of histopathological changes including cell debris in the ducts, vacuolization of the epithelial cells, oligospermia, and epithelial disruption in the proximal caput epididymidis. At 12.5 mg/kg, an increase in the incidence of histopathological changes of the epididymidis was found. There were no treatment-related effects at 3.125 mg/kg. These results show that 7-day repeated oral administration of epichlorohydrin to male rats results in adverse effects on sperm motility, sperm morphology, and epididymal histology at $\geq$ 12.5 mg/kg/day.

Lowering the concentration affects the migration and viability of intracerebroventricular-delivered human mesenchymal stem cells

Kim, Hyeong Seop,Lee, Na Kyung,Yoo, Dongkyeom,Lee, Jeongmin,Choi, Soo Jin,Oh, Wonil,Chang, Jong Wook,Na, Duk L. Elsevier 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Due to their widely known therapeutic benefits, mesenchymal stem cells have been proposed as a novel treatment option for a wide range of diseases including Alzheimer's disease. To maximize these benefits, critical factors such as delivery route, cell viability, and cell migration must be accounted for. Out of the various delivery routes to the brain, the intracerebroventricular (ICV) route stands out due to the widespread distribution that can occur via cerebrospinal fluid flow. The major objective of this present study was to observe how altering cell concentration influences the migration and viability of human umbilical cord blood derived-mesenchymal stem cells (hUCB-MSCs), delivered via ICV injection, in the brains of wild-type (WT) mice. C3H/C57 WT mice were divided into three groups and were injected with 1 × 10<SUP>5</SUP> hUCB-MSCs suspended in varying volumes: high (3 μl), middle (5 μl), and low (7 μl) concentrations, respectively. Lowering the concentration increased the migratory capabilities and elevated the viability of hUCB-MSCs. These results suggest that cell concentration can affect the physiological state of hUCB-MSCs, and thus the extent of therapeutic efficacy that can be achieved.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cell concentration must be considered for stem cell therapy. </LI> <LI> More widespread distribution in the brain is achieved with ICV injections of lower cell concentrations. </LI> <LI> A higher number of engrafted cells was observed with ICV injections of higher cell concentrations. </LI> <LI> Lowering concentration increased viability and enhanced migration. </LI> </UL> </P>

Anti-Fibrotic Effect of Urushiol (Rhus Verniciflua Stokes) on Thioacetamide-Induced Liver Fibrosis of Mice Model

( Hyeong Seop Kim ),( Ye Rin Choi ),( Mi Gun Hong ),( Min Jea Shin ),( Sang Jun Yoon ),( Na Young Lee ),( Hyun Ji Ye ),( Sang Hak Han ),( Dong Joon Kim ),( Gi Soo Youn ),( Ki Tae Suk ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Liver fibrosis constitutes a serious problem worldwide due to its rapidly leads to cirrhosis, and even cancer. However, there is no clear medical treatment other than treating the cause. Urushiol has been known to have anti-inflammatory and anti-oxidative effects on various disease. We investigated the effect of urushiol on liver fibrosis induced by thioacetamide (TAA). Methods: Thirty C57BL/6 mice were randomly divided into 3 groups and urushiol was dissolved in vehicle (DMSO) for in vivo experiments: (1) control group; (2) TAA (normal saline as solvent, 300 mg/kg/day, 3 times/week, i.p.) + vehicle (gavage). (3) TAA (300 mg/kg/day, 3 times/week, i.p.) + urushiol (0.128 mg/ mL/day, 28 times, gavage). These groups were analyzed by immunohistochemical staining, reverse transcription polymerase chain reaction, and microarray. Results: The TAA + Urushiol group improved the deposition of fibrillar collagen (Sirius Red (%) 1.55 ± 0.25 vs. TAA, P<0.0001), Fibrosis stage reduced (1.64 ± 0.20 vs. TAA, P<0.0001) and Inflammation activity decreased concurrently (1.17 ± 0.16 vs. TAA, P<0.0001). The analysis of mRNA expression showed that Col1a1 (2.30-fold change vs. TAA, P<0.0001) and TIMP-1 (1.48-fold change vs. TAA, P<0.05), IL-6 (3.03- fold change vs. TAA, P<0.0001), JAK2 (0.89-fold change vs. TAA, P<0.05), STAT3 (3.26-fold change vs. TAA, P<0.0001) downregulated significantly. As shown microarray analysis, downregulation of Col1a1 (1.12-fold change vs. TAA, P<0.05), Chka, lipid metabolic process gene (2.21-fold change vs. TAA, P<0.001), Pnpla3, patatin-like phospholipase domain containing 3 (1.57-fold change, P<0.001) Contrast, Orm2, regulation of immune system process gene (2.28-fold change vs. TAA, P<0.001) Saa1, serum amyloid A (2.25-fold changes vs. TAA, P<0.05), Saa2 (3.09-fold change, P<0.001) Conclusions: Our results indicated that urushiol has therapeutic effect on liver fibrosis. Although it is needed more clinical research, urushiol can be a potential agent for the treatment of liver fibrosis.