Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Reliability Considerations of Hybrid Power Systems for Critical Facilities

Choi, Se Jin · Ham, Young Hwan · Kim, Myung Chin 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.27 No.2

본문참고

비복신경이식을 이용한 긴 거리의 말초신경 결손부 수복

이종호,이세영,김명진,이은진,안강민,김성민,최원재,명훈,황순정,서병무,최진영,정필훈 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.2

The surgery of oral and maxillofacial area poses the risk of cranial nerve damage such as trigeminal nerve or facial nerve. Inferior alveolar nerve is prone to damage in the third molar extraction, implant installation, orthognathic surgery, open reduction and rigid fixation, and tumor ablation surgery. On the other hands,facial nerve is likely to be damaged or sacrificed with trauma or parotidectomy. In case of inferior alveolar nerve injury, the incidence is reported to be about 1.3%. The nerve function will almost recover in minimal damage, but it won't recover at last in total damage of a part of nerve unit. In latter cases, nerve regeneration in intended by allograft as nerve substitute or various route of merve condit. But the recovery with autograft is believed to be most relialbe mrthod in the rapair of long-span(longer than 15㎜)nerve defect. We have performed autologous sural nerve graft in the repair of nerve defect, which is caused by resection of benign or malignant tumor. Hereby we report the method of nerve harvesting, recovery of defected peripheral nerve and the complications of donor site with the discussion of sural nerve anatomy.

飽和度에 따른 三軸壓縮强度의 特性 硏究

최명진,고영현,상현규,김팔규 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

Lately,the study of the soil behavior and mainly related to the degree of saturation. But most soils in the ground actually exist not in the saturated condition but in the unsaturated one. The foundation bases adn the soils used for construction material are almost such unsaturated soil as granite soil in Korea. The griaxial test are used in order to examine the strength characters of the unsaturated soil in this research, reappeared in-situ field condition for the undaturated soil through the UU-test in the laboratory and examined strength character. The variation of shear strength accrding to the increase of the degree of saturation is measured as the result and the proposed formula are suggested

Rowe Cell 을 利用한 粘土試料 샘플링의 攪亂程道 推定

최명진,김민욱,전남수,김필규 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

Recently many studies which related to effective sampling method have been progressed and valuable data accumulated in foreign country. But, domestic technique is insufficient to evaluate the degree of disturbance so it is difficult to seek soli parameter. Thus the grasp of the degree of disturbance is effential to increase accuracy of reasonable parametern from laboratory tests. Also, the degree of disturbance of it was determined. Oedometer tests and Rowe cell consolidation rests reappeared in -situ state were conducted. And its result was compared and analyzed to deduces the degree of disturbance. From these tests, It can be inferred that the compression index generally is not affected and swelling index(C )and preconsolidation load (P )are increased because of the disturbance of soil. Also, it can be considered that consolidation curves given by two tests is little affected from desturbance. Conclusively, the results of two tests indicated that void ratio(e) and swilling index is independent of the degree of disturbance and it is reasonable to evaluate the degree of disturbance through it.

내부 유체 유동속도와 지지점 변화에 따른 굴곡파이프의 고유진동수

최명진 경희대학교 산학협력기술연구원 1998 산학협력기술연구논문집 Vol.4 No.-

Based upon the Hamiton's principle, the equations of motions are derived, and the finite element equation is constructed to solve the eigenvalue problem for curved pipe structures. To confirm the scheme used in this study, the results are compared with the results of commercial software ANSYS. When the initial tension is neglected in cured pipes, the natural frequencies took place. However, when the initial tension taked into account, the natural frequencies are not changed with the change o the flow velocity. In free vibrational simulation of pipe systems, it is necessary to calculate the initial state force due to the velocity and the pressure of the fluid flow from the equilibrium, then the force should be included in the equation of motion of the systems to get more accurate natural frequencies. The mechanical properties like stiffness or the location of pipe supports are need to be changed to avoid resonance. The natural frequencies are to be isolated from the frequency range of dominant vibration modes. The angles of elbows do not affect the change of the fundamental natural frequency, but affect the change of the third or higher natural frequencies.

유속과 곡관부의 형상 변화를 고려한 파이프 계의 자유진동 해석

최명진,임경식,이동명 경희대학교 산학협력기술연구원 2000 산학협력기술연구논문집 Vol.6 No.-

A simulation is performed to study the change of the natural frequencies of curved pipe systems containing fluid flow, for different angles and geometry of the pipe systems. Based upon the Hamilton's principle, the equations of motions are derived. This paper consider an initially deformed state caused by the fluid flowing through the pipe at a constant velocity. This deformed state gives rise to initially tension force in the pipe. When the initial force is neglected in cured pipes, the natural frequencies are reduced as flow velocity increases. However, when the initial force is considered, the natural frequencies changed a little with the change of the flow velocity for changing shape(curvature, angle) of curved pipes. In free vibrational simulation of pipe systems, it is necessary to consider the initial force due to the flow velocity and the geometric shape of pipes. Like this, the initial force should be included in the equation of motion of the systems to get more accurate natural frequencies and the change of shape of curved pipes should be considered.

주의력결핍 과잉행동장애 아동에서 틱 장애 동반 여부에 따른 확산 텐서 영상 비교 연구

최지욱,임명호,이창화,박진균,손정우,심세훈,유인규,강현수,정범석 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.5

Objectives : Diffuse tensor imaging (DTI) was applied to explore the difference in regional distribution and extent of white matter (WM) abnormalities in boys with Attention-deficit/hyperactivity disorder (ADHD) versus boys with comorbid ADHD and tic disorders. Methods : Fifteen boys with ADHD (mean age 9.3 +1.8), 24 ADHD boys with chronic tic disorder or Tourette's disorder(9.9 =b 1.2) and 9 age-, gender-matched controls (9.2 d= 1.8) received DTI assessments. Fractional Anisotropy (FA) maps of WM were compared between groups with a voxel-wise analysis after intersubject registration to MNI space. Results : Bo groups, ADHD group and ADHD with tic disorder group, commonly showed decreased FA than healthy control group in left cerebellar middle peduncle and right frontal lobe, increased FA in right middle occipital WM. In the common areas of left cerebellar middle peduncle and right middle occipital WM, comorbid group showed broader areas of significant FA. The comorbid group also showed increased FA in right cerebellar peduncle, additionally. Conclusion : The findings in ADHD group support previous ADHD hypothesis of the functional abnormalities in corticocerebellar circuit, and suggest that ADHD might have more complicated pathology of neuronal circuit including occipital visual system. The comorbid group showed common areas of overlapping but more extensive abnormalities and also had additional WM abnormalities. ADHD with chronic tic disorders may represent a severe form of ADHD with additional regions of abnormal connectivity.

하악골 골절후의 골치유과정에 있어서 방사선학적 추적조사

최진영,김명호,허경희,서병무,이종호,정필훈,김명진 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.5

The results of radiographic study of healing of fractured mandible are often inconsistent with clinical evaluation of bone healing pattern of it. In addition, there is inconsistency between radiographic studies. We think if such inconsistency is resolved, clinical assessment combined with radiologic evidence can be used to guide to more accurate clinical management. The purpose of this study is to evaluate radiological healing pattern of mandible, and to apply its result to clinical use. Panoramic view was used to analyze the change of radiographic finding in 35 patients (27 male, 8 female, ranging 17 to 53 years old) treated for mandibular fracture at the department of oral & maxillofacial surgery, SNUH in the period 1991 to 1998, Patients are all treated by rigid internal fixation using monocortical miniplate. Within 9 weeks, no change is observed at fracture site. Osteogenic change is observed at various time interval (3 to 21 weeks). We have found radiographic change of healing is observed 4 to 6 weeks later to clinical healing, According to this result, we recommend radiographic follow-up at 4 week, 10 week, 10 month.

대퇴골두의 자유진동해석

최명진,백승민 慶熙大學校 材料科學技術硏究所 2000 材料科學技術硏究論集 Vol.13 No.-

Three dimensional finite element model of human femoral head is generated based upon DICOM file which is standard medical image file. Connectivity and Jacobian ratio are checked to input the generated data to commercial finite element software. Eigenvalues and the corresponding eigenvectors are evaluated through free vibration analysis. Free vibration characteristics of femoral head are expected to be utilized a fundamental data to develope and design artificial femurs.