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ABO 및 RhD 혈액형 검사와 비예기항체 선별검사를 위한 자동화장비 QWALYS-3의 평가
고영현,김지명,구선회,임진숙,박영춘,권계철 대한수혈학회 2011 大韓輸血學會誌 Vol.22 No.2
Background: One hundred years after the discovery of the ABO blood group system by Landsteiner,agglutination is still the most widely used method for determining the blood grouping and for antibody detection. Yet the demand for suitable automated systems is obvious in the field of transfusion. QWALYS-3 (DIAGAST,Loos Cedex, France) is a fully automated system for determining the ABO and RhD grouping and for antibody screening. We evaluated the ABO and RhD grouping and antibody screening test of the QWALYS-3. Methods: The ABO and RhD grouping and the antibody screening test were performed by the QWALYS-3system and the results were compared to the manual methods for the ABO and RhD grouping and to the micro column agglutination method (DiaMed-ID system) for antibody screening. Results: For 574 of 574 clinical samples, there was complete concordance between the QWALYS-3 and the manual methods for determining the ABO and RhD grouping. The concordance rate between the QWALYS-3and the micro column agglutination method for antibody screening in 210 clinical samples was 97.1%. Conclusion: The QWALYS-3 system had a good concordance rate compared to the manual method and the micro column agglutination method. The QWALYS-3 system was robust and it gave accurate results during this evaluation. The QWALYS-3 system will contribute to achieving full automation of all the necessary blood bank tests.
고영현,이민아,신소영,구선회,송정훈,임진숙,권계철 대한진단검사의학회 2011 Laboratory Medicine Online Vol.1 No.4
Background: Toxoplasma gondii can cause devastating disease in the fetus and newborn infant. Serologic testing of pregnant women for Toxoplasma-specific antibodies can be used to identify those women at risk of transmitting Toxoplasma gondii infection. In Korea, despite a few reports on the seroprevalence of Toxoplasma (Toxo) antibody, the incidence of acute or chronic toxoplasma infection during pregnancy has not been well established. We performed a prospective screening for Toxoplasma antibodies to obtain a basic epidemiological data on the seroprevalence of acute and chronic toxoplasma infection. Methods: During a 6-month period, 787 pregnant women at various weeks of gestation were enrolled in the prospective study. Toxo IgG and IgM antibodies were determined by the Abbott AxSYM Toxo IgG and IgM assays. Serum specimens showing positive results of both IgG and IgM antibodies were further tested using the Abbott ARCHITECT Toxo IgG Avidity test. Results: The seropositivities of Toxo-specific IgG and IgM antibodies in this cohort were 2.3% (18/787) and 0.1% (1/787), respectively. No woman showed positive results for both Toxoplasma IgG and IgM antibodies. One specimen showing IgG positive and IgM grayzone results was tested by Toxo IgG avidity test and a low avidity test result (9%) was obtained, suggesting a possible acute primary infection. Conclusions: This study was the first trial on the investigation of the seroprevalence of both IgG and IgM antibodies in Korea, and we found that the seroprevalence of the antibodies was lower than that previously reported.
모래다짐말뚝(SCP) 개량지반의 응력분담비에 관한 연구
고영현,김병일,박용원 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
Lots of soil improvement methods has been developed in order to increase soil properties of soft ground such as newly reclaimed land The sand compaction pile(SCP), which forms a composite ground by driving sand piles into soft ground, is one of the commonly used soil improvement techniques especially for soft clay deposit. In desinging SCP method, it is very important to determine the stress concentration ratio of composite ground. In this study, 3-dimensional analyses were carried out using single and group pile models to evaluate the stress concentration ratio with the area replacement ratio and the stiffness ratio. PENTAGON_3D software is used to analyze the composite ground using modeling with single pile of unit cell concept and group piles. As a result, the distribution of the predicted stress concentration ration with the stiffness ratio(Es/Ec) and the area replacement ratio is around 2.5 ~ 3.5