실험적 녹농균성 각막염에 대한 Cefotetan의 치료 효과

오명주,박정규,백태현,최대경 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

This study was carried out for the purpose of evaluation of effect of cefotetan against the pseudomonas induced keratitis. The corneal infection caused by pseudomonas organisms have a poor prognosis and their increased prevalence and general resistance to therapy make them a serious problem for to ophthalmologists. So, the author introduced experimental keratitis in the rabbit cornea by Ps. aeruginosa and Compared effects of the cefotetan and tobramycin. The cefotetan is a new antibiotic of cephalosporin system which is effective in vivo and vitro against not only gram positive but also gram negative organism and is stable against the β-lactamase. The results obtained were as follows: 1. Clinically, cefotetan and gentamicin were both effective against experimentally induced .pseudomonas keratitis and there was no significant difference between the efficacy of cefotetan and that of tobramycin. 2. In culture study, the test after treatment showed significantly fewer colonies on the platesfrom the cefotetan, tobramycin treated groups than those from the saline treated group, and there was no significant difference between cefotetan treated, and tobramycin treated group.

결핵균 30-kDa와 32-kDa 단백항원의 분리정제와 마우스 비장세포 증식에 미치는 영향

백태현,오명주,김화중,조은경,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Identification and characterization of individual components of M.tuberculosis have long been a focus of research on tuberculosis. The 30-kDa and 32-kDa antigens are major constituents of M bovis BCG and M. tuberculosis culture fluids. Because 30-kDa and 32-kDa antigens are partially identical, these antigens are difficult to purify in large amounts by biochemical techniques. This study was performed to purify the 30-kDa and 32-kDa antigens complexes primarily purified by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 gel filtration. And then further purification for separation of the two antigens was accomplished on preparative isoelectric focusing. Recovery of 30-kDa and 32-kDa antigens during above the purification procedures were 28% and 14%, respectively, and 147.0 and 59.8-fold purification were showed, respectively. On silver stained SDS-PAGE gels, the purified 32-kDa antigen gave a single band at 32-kDa molecule, while 30-kDa antigen gave one major band at 30-kDa molecule and faint additional band at 32-kDa. The pI of 30kDa-and 32-kDa antigens were 4.3 and 4.6, respectively. The partial identity between these two antigens was observed through the same pattern of reactivity of antigens in the ELISA and precipitation pattern of immunodiffusion. We also examined the immunological activities of both purified antigens by splenocyte proliferation of BCG-infected mice. Proliferative response to the 30-kDa and 32-kDa antigens did not show significant difference. However, macrophage-like cells existed in spleen cells from BCG-infected mice were involved in the suppression of the proliferative response to mycobacterial antigen. These results suggest that the 30-kDa and 32-kDa antigens could be effectively purified by the IEF and reactions of partial identity between the two antigens were found. However, 30-kDa antigen was the more immunogenic antigen than 32-kilodalton.

금강유역 수질오염 실태조사

최신석,오희목,강명규,조통래,이규승,유종국,이영하 忠南大學校 環境問題硏究所 1988 環境硏究 Vol.6 No.1

조사 구역 상류 지점의 평균 수질은 pH가 7.1, DO가 8.3mg/ℓ, BOD가 1.7mg/ℓ, COD가 1.7mg/ℓ, SS가 15.4mg/ℓ, 분변성 대장균이 1.1x10³ MPN/100ml고 상수원 수 1급 내지 2급의 양호한 상태이고 하류지점의 수질은 pH가 6.8, DO가 7.7mg/ℓ, BOD가 2.2mg/ℓ, COD가 2.4mg/ℓ, 분변성 대장균이 1.7x10³MPN/100ml로 상수원 수 2급 내지 3급의 상태를 나타내 하류로 내려가면서 오염도가 증가하는 것으로 나타났다. 청주시의 영향을 크게 받는 미호천 하류의 평균수질은 pH가 7.2, DO가 6.3mg/ℓ, BOD가 16.2mg/ℓ, COD가 15.1mg/ℓ, 분변성 대장균이 1.6x10??MPN/100ml로 심각한 오염 상태를 보여 청주시와 대전시가 금강 본류의 주 수질 오염원으로 나타났다. 영양 염류 (NH₃-N, NO₂-N, NO₃-N, PO₄-P)와 무기성 유해 성분 (Cl-, CN-, F-)의 함량은 지천의 일부 지점을 제외하고는 극히 낮은 수준으로 검출되었으며, 유기인, 페놀류, PCB 성분은 조사기간 중 전혀 검출되지 않았다. 또한 Fe, Zn, Cu, Mn, Cr, Pb, Cd, Hg등의 중금속은 검출되지 않거나 하천 수질 기준 또는 식용수 수질 기준의 허용치를 초과하지 않는 양호한 수준으로 나타났다.

Polyurethane의 혈전방지처리가 Staphylococcus epidermidis의 정착에 미치는 영향

김성민,이규백,백경란,오명돈,민병구,최강원 대한감염학회 1996 감염 Vol.28 No.1

목적 : 최근 생체재료로 만든 각종 카테타와 인공관절, 인공심장 등 인공장치의 사용이 점차 증가하고 있으며, 인공장치의 사용을 제한하는 가장 큰 합병증은 생체재료를 중심으로 일어나는 감염과 혈전증이다. 그러므로 인공장치를 장기간 사용하기 위해, 이들 합병증을 예방할 수 있는 생체재료를 개발하는 것이 중요한 과제가 되고 있다. 인공장치는 체내에 들어가서 혈액과 접촉하게 되면, 혈장단백질과 혈소판이 부착하고 활성화되어 혈소판-섬유소 혈전을 만든다. 이렇게 형성된 혈전은 세균의 부착을 촉진하고, 부착한 세균이 인체방어기전이나 항균제의 영향을 받지 않고 증식 할 수 있는 공간이 된다. 또한 정착한 세균은 혈액과 접촉하여, 혈전의 형성을 촉진한다. 즉, 혈전과 세균정착은 서로 영향을 주면서 인공자치의 오랜 사용을 제한하게 된다. 폴리우레탄에 설폰산과 알부민을 표면처리하면 혈전의 형성이 감소하는 효과가 있다는 것은 보고된 바 있으나, 혈전방지를 위해서 표면처리에 사용한 설폰산, 알부민이 세균 정착 및 감염에 미치는 영향에 대해서는 아직 잘 알려져 있지 않다. 이에 저자들은 설폰산, 알부민, 파라벤으로 처리한 폴리우레탄이 세균의 정착에 미치는 영향에 대해 알고자 본 연구를 시행하였다. 방법 : S. epidermidis RP12 부유액(?/mL)에 대조 폴리우레탄과 설폰산과 알부민, paraben으로 각각 처리한 폴리우레탄을 원반모양으로 만들어 담근 다음, 24시간 또는 48시간 동안 배양하였다. 세균 정착의 정도는 세균 집락수 및 SEM 소견으로 판정하였다. 결과 : 24시간 째 및 48시간 째의 세균집락수는 대조 폴리우레탄이 각각 8.3x10⁴/mL, 1.1×?/mL이었고, 파라벤 처리 폴리우레탄이 각각 9.3x10⁴/mL, 2.1x10⁴/mL (p<0.05), 설폰산 처리 폴리우레탄이 각각 1.2×?/mL, 8.2x10⁴/mL,(p<0.05), 알부민 처리 폴리우레탄이 각각 7.3×?/mL, 1,4×?/mL (p<0.05)이었다. 즉, 세균 집락수가 파라벤 처리 폴리우레탄에서는 유의하게 감소하였으나, 설폰산 처리 폴리우레탄에서는 증가하였다. SEM 소견에서는 설폰산 및 알부민 처리 폴리우레탄에서 세균 정착의 감소를 관찰하지는 못하였다. 결론: 본 연구는 in vitro 실험으로, 파라벤으로 처리한 폴리우레탄은 세균의 정착을 감소시키는 효과가 있었으나, 항혈전성 폴리우레탄은 세균 정착을 감소시키지 못하였다. 인체내에서는 세균과 혈소판, 혈장단백질이 복잡하게 반응을 하면서 혈전과 세균 정착이 서로 영향을 주므로, 인체내에서는 항혈전성 폴리우레탄이 혈전 형성을 감소시키고, 2차적으로 세균 정착을 감소시키고 감염을 예방하는 효과가 있을 가능성도 있으므로 이에 대한 연구가 진행되어야 하겠다. Background : Infection and thromboembolism are major complications of prolonged use of prosthetic devices, such as various catheters, prosthetic joints, and artificial hearts, frequently resulting in severe morbidity, or death. Therefore, the prevention of infection and thrombosis is an important subject in developing new biomaterials of prosthetic devices. The first event on insertion of a prosthetic device into the blood is protein adsorption and activation with platelet adhesion, activation and aggregation, leading to the formation of platelet-fibrin thrombi. The thrombi are susceptible to bacterial incorporation and protect the bacteria from the effects of circulating antibodies, phagocytic cells, and antibiotics. Furthermore, adherent bacteria may induce fibrin and platelet adhesion. Sulfonated polyurethane and albumin-immobilized polyurethane were documented to diminish the adhesion and shape-change of platelet on the polymer in comparison with plain polyurethane, in vitro. The objective of this study was to evaluate the effect of these antithrombotic biomaterials on the bacterial colonization. Methods : Plain, sulfonated, albumin-immobilized, and paraben-incorporated polyurethane(PU) films were prepared as a disk form. Staphylococcus epidermidis RP12 was suspended at a concentration of ?/mL and four types of polymeric disks were immersed in the bacterial suspension. After incubation for 24 and 48 hours, the bacterial colonization on the each disk was determined by the colony forming units(CFU) counting method. The colonized disks were examined by scanning EM. Results : The paraben decreased significantly the number of CFU on PU surface to 2.1x10⁴/mL after 48 hours, compared with 1.1×?/mL on plain polymers. The number of CFU on sulfonated or albumin-immobilized PU was 8.2x10⁴/mL, and 1.4×?/mL, respectively. SEM micrographs showed no reduction of colonization on antithrombotic PUs. Conclusion : Antithrombotic treatments of polymers did not decrease the bacterial colonization on polymers, in vitro, that was compared to the decreasing effect of paraben. However, because the thrombi and bacteria interact with each other, the overall effect of the antithrombotic polyurethanes on the bacterial colonization and infection should be studied in vivo.

성인간호학회지 논문(1989-2000)의 내용과 경향 분석

서문자,김소선,송미순,이명선,오의금,신경림,김옥수,이숙자,이영자,오효숙,정재심,김철규 성인간호학회 2001 성인간호학회지 Vol.13 No.4

"Journal of Korean Academy of Adult Nursing" has been published since 1989 and more than 330 articles have been published up 2000. We reviewed all the articles published in this journal to analyze the contents and trend of published articles and to find out the way of improvement both in quantity and quality of studies in this area. The results are as follows : 1) Ninety percent of articles were quantitative studies. Experimental design was used in 16.1% and quasi-experimental design was adapted in 73.6% of articles. Qualitative research were 10.3% of total articles reviewed. 2) Only 5.1% of articles described theoretical framework and 73.6% of experimentally designed articles included research hypotheses. 3) Among experimental studies, only one was true experimental studie design. Eighty nine point three persent had descriptive survey method among non-experimental studies. Amog the thirty four qualitative studies, phenomenologic(73.5%), grounded theory(14.7%), and ethnographic (11.8%) design were used. 4) Most of research subject were patients and few healthy people, healthcare worker, and patient family. 5) Psychosocial data collection method which used questionnaire were 53.7% and interview. physical measurement, observation were used in 22.8%. 8.2% 5.4% of articles respectively. 6) Total number of keywords were 692, and average number of key words per article was 2.2. Health-related keyword was the most frequently used according to four metaparadigm of nursing (human, health, nursing, and environment). Environment related keyword was rarely appeared. The number and quality of nursing research in "Journal of Korean Academy of Adult Nursing" have been improved continuously. It is difficult to find out special trend or characteristics which is unique in this journal because of relatively short history of adult nursing studies. This review study was performed to analyze adult nursing studies only in the view of quantitative aspect, but it is needed to analyse qualitatively to find philosophical or theoretical trend in nursing.

Morphological and SERS Properties of Silver Nanorod Array Films Fabricated by Oblique Thermal Evaporation at Various Substrate Temperatures

Oh, Myoung-Kyu,Shin, Yong-Seok,Lee, Chang-Lyoul,De, Ranjit,Kang, Hoonsoo,Yu, Nan Ei,Kim, Bok Hyeon,Kim, Joon Heon,Yang, Jin-Kyu Springer US 2015 NANOSCALE RESEARCH LETTERS Vol.10 No.1

<P>Aligned silver nanorod (AgNR) array films were fabricated by oblique thermal evaporation. The substrate temperature during evaporation was varied from 10 to 100 °C using a home-built water cooling system. Deposition angle and substrate temperature were found to be the most important parameters for the morphology of fabricated films. Especially, it was found that there exists a critical temperature at ~90 °C for the formation of the AgNR array. The highest enhancement factor of the surface-enhanced Raman scattering (SERS), observed in the Ag films coated with benzenethiol monolayer, was ~6 × 10<SUP>7</SUP>. Hot spots, excited in narrow gaps between nanorods, were attributed to the huge enhancement factor by our finite-difference time-domain (FDTD) simulation reflecting the real morphology.</P>

Ultimate sensing resolution of water temperature by remote Raman spectroscopy

Oh, Myoung-Kyu,Kang, Hoonsoo,Yu, Nan Ei,Kim, Bok Hyeon,Lee, JoonSeok,Hyung, Gi Woo OSA OPTICAL SOCIETY OF AMERICA 2015 Applied Optics Vol.54 No.10

슬관절경 수술 후 관절강내 Bupivacaine 에 첨가하는 Neostigmine 과 Morphine 의 진통효과

오인영,박춘식,김태엽,김인규,신명근,이상엽 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.1

Application of Multiparametric Flow Cytometry (FCM) to Enumerate the Diagnosis of Pseudomonas aeruginosa and Escherichia coli

Myoung Goo Hwang,Jung Woo Oh,Hiroyuki Katayama,Shinichiro Ohgaki,Jin Kyu Cho 대한환경공학회 2012 Environmental Engineering Research Vol.17 No.1

In this study, multiparametric flow cytometry (FCM) was installed to enumerate the diagnosis of Pseudomonas aeruginosa ATCC 10145 and Escherichia coli K12 (IFO 3301). The nucleic acids (DNA/RNA) were double stained by a LIVE/DEAD bacLight viability kit, involving green SYTO 9 and red propidium iodide (PI), based on the permeability of two chemicals according to the integrity of plasma membrane. As the results showed, the gate for dead bacteria was defined as the range of 0.2 × 100 to 6.0 × 101 photo multiplier tube (PMT) 2 fluorescence (X-axis) and 2.0 × 100 to 2.0 × 102 PMT 4 fluorescence (Y-axis), and the gate for live bacteria was defined as the range of 6.0 × 100 to 6.0 × 102 PMT 2 fluorescence (X-axis) and 2.0 × 100 to 4.0 × 102 PMT 4 fluorescence (Y-axis). In the comparison of the number of the tested bacteria detected by FCM (viability assessment) and plate culture (cultivability assessment), the number of bacteria detected by FCM well represented the number of bacteria that was detected by the colony forming unit (CFU) counting method when bacteria were exposed to isopropyl alcohol and silver/copper cations. Consequently, it is concluded that the application of FCM to monitor the functional effect of disinfectants on the physiological status of target bacteria can offer more rapid and reliable data than the plate culture colony counting method.

Notes : Biochemical Characterization of an ABC Transporter Gene Involved in Cephabacin Biosynthesis in Lysobacter lactamgenus

( Myoung Jin Park ),( Jei Oh Yon ),( Si Kyu Lim ),( Dewey D. Y. Ryu ),( Doo Hyun Nam ) 한국미생물 · 생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3