Delayed Hydride Crack Velocity of Zirconium Alloys with the Direction of an Approach to Temperature

KIM, Young Suk,KIM, Kang Soo,CHEONG, Yong Moo Atomic Energy Society of Japan 2006 Journal of nuclear science and technology Vol.43 No.9

<P>The delayed hydride cracking (DHC) tests were conducted on Zr-2.5Nb compact tension specimens with the test temperatures reached by a heating and a cooling. The Zr-2.5Nb specimens were either furnace-cooled or water-quenched after a hydrogen charging treatment to contain 10 to 100 ppm H. On an approach to the test temperatures by a cooling, both the Zr-2.5Nb specimens showed the DHC velocity increasing with an increasing temperature over a temperature range of 100–300°C, irrespective of the cooling rate. However, on an approach to the test temperatures by heating, the furnace-cooled Zr-2.5Nb showed a DHC arrest at temperatures over 180°C and no DHC at 250°C, and the water-quenched ones did have a DHC growth, even at 250°C. Using Kim’s DHC model we elucidate the DHC arrest in the furnace-cooled Zr-2.5Nb at temperatures over 180°C and the DHC growth in the water-quenched specimen, even at 250°C, upon an approach by a heating.</P>

Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.

Mirizzi 증후군의 변형된 분류와 치료

김형철,강길호,채만규,김성용,백무준,이문수,박상흠,이문호,김창호,송옥평,조무식,박희주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Purpose : The Mirizzi syndrome is relatively rare and preoperative diagnosis of this disease is difficult. In 1978, Morelli suggested the subclassification of the Mirizzi syndrome into acute or chronic form. We experienced 5 cases of acute form. We analysed clinical features, preoperative radiologic findings and operative findings of 18 cases including acute forms which were diagnosed as Mirizzi syndrome and should suggest the modified classification of Mirizzi syndrome for choice of appropriate treatment. Method : From January 1995 to December 1998, 18 cases, of which 8 cases were diagnosed at Soonchunhyang University Chunan Hospital, and 10 cases were reported in the Korean Journal were retrospectively analysed with regard to clinical features, preoperative radiologic findings and operative findings. According to the clinical features, whole cases were divided into type Ⅰ(acute form) and type Ⅱ(chronic form) and then each type of cases were subclassified according to preoperative radiologic findings and operative findings. Results : Of 18 cases there were 5 cases in type Ⅰ(27.8%), 13 cases in type Ⅱ(72.2%). Type Ⅱb was most common. Type Ⅰa cases were treated only with cholecystectomy. We applied cholecystectomy, T-tube choledochostomy and patch technique in type Ⅰb and thpe Ⅰc cases. Cholectystectomies including removal of gallstones and internal drainage procedures were done in type Ⅱ chronic forms. Conclusion : The acute form(Type Ⅰ) of Mirizzi syndrome was suggested by Morelli might be subclassified into typeⅠa,Ⅰb and Ⅰc following the presence of the necrotic defect in common hepatic duct. Through the modified classification of Mirizzi syndrome based on clinical feature, preoperative radiologic findings and operative findings, we can choice appropriate treatment.

馬蹄鐵腎의 一例

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

A case of horseshoe kidney which was found during dissection of cadaver was reported with a brief review of literatures. The main findings were as follows; 1. This horseshoe kidney was supplied by the 4 renal arteries were obtained from the aorta and 1 renal artery was originated from the common iliac artery. 2. The pelvis of the horseshoe kidney were placed anteriorly and the ureters were passed anteriolateral surface to the renal isthmus. 3. In the horseshoe kidney, the renal stone was not observed and the isthmus was consisted of the renal parenchyma.

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

고양이, 돼지 및 오리의 망막에 존재하는 S-100 단백질의 분포에 대한 면역화학적 연구

김무강,김상근,이근좌 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-

소의 뇌에서 처음으로 분리된 S-100 protein이 각 동물 망막에 존재하는 상태를 규명하기 위하여 S-100 protein antiserum을 이용하여 고양이,돼지, 오리의 망막을 면역화학적 방법으로 처리하여 그 분포상태를 조사하였던 바 다음과 같은 결론을 얻었다. 1. 고양이, 돼지, 오리의 망막에 분포하는 S-100 protein의 분포부위와 그 정도가 동물에 따라 서로 달랐다. 2. 고양이와 돼지에서는 각신경세포층 및 각 망상층에 모두 존재하였으나 오리에서는 각신경세포만 존재 하였다. 3. 각 동물 망막에 존재하는 S-100 protein의 분포를 규명하는 것은 앞으로 각 동물의 망막연구에 필요한 각 층들을 구별 하는데 좋은 지침이 될 수 있다.

Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

韓國在來山羊의 綱膜神經節細胞數와 分布에 關한 硏究

金武剛,趙聲煥,申台均 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

The number and distribution of retinal ganglion cells in the Korean native goats was studied with whole mount preparations stained with methylene blue and thionin. The mean area of 5 retinae is 918 mm² and the mean number of total ganglion cells was 1, 602, 000. So the mean number of ganglion cells per mm² was 1,745. The density distribution of the retinal ganglion cells showed that visual streak was located about 2mm dorsal to the optic disc, and area centralis (7,700 cells/mm') was located about 10mm temporally and 2mm dorsal to the optic disc. There was drop in density of retinal ganglion cells about the circumference of the visual streak in dorsal and ventral direction. The functional significance of the findings was also discussed.

각종 동물 설유두의 형태학적인 관찰

김홍선,김무강,김원식,이영호,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The lingual papillae of the mouse, the rabbit, and the cat were observed by light microscope and scanning electron microscope The results were followings 1. The filiform papillae of the mouse were classified to typical filiform papillae, conical filiform papillae with 2 branched tips, and general conical filiform papillae. The outmost layers of the filiform papillae of the mouse keratinted well, middle layer of the anterior portion of the papillae appeaered a lot of small basophilic granules. The fungiform papillae existed between the filiform papillae with the peripheral folds of epithelia. A vallate papilla existed on the posterior portion of conical filiform papillae and their shape was lenticuiar. 2. The filiform papillae of the rabbit revealed various type (5 types). Keratin layer of the papillae existed vertical to long axis of the papillae on the posterior portion of the papillae. Stratified squamous epithelium on the anterior portion of the papillae existed with a few keratin layers and the intercellular materials of strong PAS positive reaction appeared between the these cells. The fungiform papillae of the rabbit existed between the filiform papillae and had a few peripheral folds. The foliate papillae of the rabbit existed on the lateral portion of tongue root, consisted of the paralleled folds. Taste buds located in the mid-lateral portion of the papillae concentrically. 3. The filiform papillae on the anterior portion of the cat tongue shaped anterior and posterior projection, the filiform palillae on the middle portion had a projections of columnar shape, which papillae surface appeared as onion surface. The conical filiform papillae on the posterior portion had many folds on the lower portion of the papillae. The fungiform papillae of the cat existed between the filiforr papillae with a columnar shaped projectionand proturded from the surface. The vallate papillae of the cat existed between the conical filiform papillae and their shape was typical form of vallate papillae.

소 卵胞의 體外培養에 의한 卵子의 體外成熟과 體外受精에 관한 硏究

金相根,南潤伊,山根倫子,金武剛 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

This study was to investigate the effects of the follicular culture from which the oocytes origenate on their subsequent in vitro maturation. The follicular oocytes were cultured in TCM-199 medium containing FCS, hormones, BFF for 24-30 hrs in CO^2 incubator with 5% CO^2 in air at 38.5℃ and then matured oocytes were again 18-20 hrs with motile capacitated spermatozoa in the BO solution containing 100 ㎍/㎖ of heparin. The results obtained in these experiments were summarized as follows : 1.Ovarian follicles were isolated and cultured according to size 1-2 ㎜, 2-4 ㎜, 4-6 ㎜ and 6-8 ㎜ for 24-30 hrs. The rates of GVBD in each group were 80.0%(40/50), 84.4%(38/45), 77.8%(35/45) and 84.0%(42/50), but oocytes maturation were supressed at anaphaes-Ⅰ or telephase-Ⅰ stage. 2. When oocytes cultured for 4 hrs at 35℃ in medium with BFF and hormones after follicular culture for 24-30 hrs, the maturation completed to metphase-Ⅱ stage. However, rates of GVBD in oocytes from follicular culture were higher than oocytes cultured in medium. 3. When oocytes from follicle cultured for 4 hrs at various temperature and culture time were cultured for 24-30hrs the rates of oocytes maturation from follicle cultured at 20℃(43.6%, 24/55), 30℃(47.3%, 26/55) and 35℃(52.7%, 29/55) were significant higher than group cultured at 4℃(24.4%, 11/45). 4. When oocytes from follicles cultured for hrs at 35℃, the rates of oocytes maturation and fertilization rates were 67.3%(101/150), 29.3%(44/150) and oocytes cultured for 24-30hrs at 35℃, thr rates of oocytes maturation and in vitro fertilization were 86.5%(122/141), 53.9%(76/141), respectively.