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Kainic Acid가 미성숙 오리 망막에 미치는 영향에 관한 전자현미경적 연구
김무강,이근좌,신태균,Kim, Moo-Kang,Lee, Geun-Jwa,Shin, Tae-Gun 한국현미경학회 1992 Applied microscopy Vol.22 No.1
In order to identify the effects of the kainic acid in the retina kainic acid (120 nmol/$5{\mu}l$) were injected through the pars plana of the ciliary body into the duck eye ball at 7th day after hatching. The animals were sacrificed by decapitation on the 1st, 4th and 10th day after injection of the kainic acid. The specimen was processed for the light and electron microscopes respectively. The results obtained were as followings 1. The inner nuclear, inner plexiform and ganglion cell layers of the retina showed marked changes in all of the groups. 2. The cells in the inner nuclear layer showed marked degenerative changes of the organelles including the pyknoses and destruction of the nucleus on the 1st day after injection. These destructive changes of the cells were progressively disappeared until the 10th day after injection. 3. After injection of the kainic acid the neurites of the inner plexiform layer were swollen at the first, and thereafter those changes were gradually disappeared until the 10th day. 4. In the ganglion cell defected by the kinic acid, the enlargement and segmentation of the endoplasmic reticulum and pyknosis of the nucleus were-observed in all the groups, although the lipid droplets were only shown in the 4th day. From the above results, the evidence suggests that kainic acid gives to the toxic effect on the nuurons of the retina with exception of the photoreceptors.
절수에 의한 Mongolian gerbil 신장조직의 변화에 관한 형태학적 연구
김무강,이근좌,정영길,송치원,이경열,박일권,이철호,이기훈,현병화,김길수,Kim, Moo-Kang,Lee, Keun-Jwa,Jeong, Young-Gil,Song, Chi-Won,Lee, Kyeng-Youl,Park, Il-Koun,Lee, Chul-Ho,Lee, Ki-Houn,Hyun, Byung-Hwa,Kim, Gil-Soo 한국현미경학회 1997 Applied microscopy Vol.27 No.4
Mongolian gerbil (Meriones unguiculatus) has been as an animal model for studing the neurological diseases such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animals. In order to accomplish the this research, first of all another divided the laboratory animals 5 groups of which each group include the 5 animals. In this study were investigated the histological structure in the kidney, measured the plasma osmolalities at the time of sacrifice of indivisual animals, and the body weights every day during water-deprived. The results obtained in this study were summarized as followings: 1. The body weights and decreasing rates of the body weight in water-deprived mongolian gerbil groups were continuosly decreased. 2. The plasma osmolalities were increased from the 5th water-deprived day, after then the gradually increasing reached nearly its equilibrium state at the 10th water-deprived day. 3. The urine volumes were abruptly decreased from the 2th water-deprived day, after then the gradually decreasing patterns were reached nearly its equilibrium state at the 10th day, and stopped the 11th day. 4. In the light microscopical observation of the kidney, glomerular capillary loop thickening, mesangial matrix increasing, sclerosis, glomerular cystic atrophy, interstitial fibrosis, tubular dilatation, mononuclear interstitial inflammation, interstitial mineralization, and hyperplasia of the collecting duct epithelium in the cortex area, were observed from the 10th water deprived day, and the lesions were gradually severe changed as the time lapse. 5. In the electron microscopical findings of the kidney, the degenerative changes of endothelial cell, podocyte and mesangial cell in glomeruli were initially observed on the 10th water-deprived day as well as the degeneration of microvilli and intracellular organelle in the renal tubules.
Localization of S-100 Protein in the Salivary Glands of Rat, Cat and Human
Kim, Moo-Kang,Yoon, Won-Kee,Lee, Keun-Joa,Ryu, Si-Yun,Cho, Sung-Whan,Kim, Sang-Keun 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-
소의 뇌에서 최초로 분리된 칼슘 결합 S-100 단백의 동물별 또는 장기별 분포 부위와 그 농도의 차이를 규명하기 위하여 S-100 단백 항혈청을 흰쥐, 고양이, 사람의 대타액선에 면역조직화학적 방법으로 처리하여 연구하였던 바 다음과 같은 결론을 얻었다. 1. 대타액선에 분포하는 S-100 단백의 분포 부위와 그 농도는 동물과 타액선의 종류에 따라 차이가 있었다. 2. 이하선의 선조관은 흰쥐, 고양이, 사람 모두에서 S-100 단백 항혈청에 의해 뚜렷이 염색되었고 사람에서는 선포 주위에 수상돌기양 세포도 염색되었다. 3. 악하선은 흰쥐에서는 점액선 선포, 고양이는 반대로 장액선 선포가 약하게 염색되었으나 사람의 선포에서는 양선포 모두에서 염색성을 관찰할 수 없었다. 4. 설하선의 선조관 상피는 각 동물 모두 염색되었으며 고양이에서는 수상돌기양세포도 염색되었고 사람에서는 점액선포는 염색되나 장액선포의 염색성은 없었다.
고양이, 돼지 및 오리의 망막에 존재하는 S-100 단백질의 분포에 대한 면역화학적 연구
김무강,김상근,이근좌 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-
소의 뇌에서 처음으로 분리된 S-100 protein이 각 동물 망막에 존재하는 상태를 규명하기 위하여 S-100 protein antiserum을 이용하여 고양이,돼지, 오리의 망막을 면역화학적 방법으로 처리하여 그 분포상태를 조사하였던 바 다음과 같은 결론을 얻었다. 1. 고양이, 돼지, 오리의 망막에 분포하는 S-100 protein의 분포부위와 그 정도가 동물에 따라 서로 달랐다. 2. 고양이와 돼지에서는 각신경세포층 및 각 망상층에 모두 존재하였으나 오리에서는 각신경세포만 존재 하였다. 3. 각 동물 망막에 존재하는 S-100 protein의 분포를 규명하는 것은 앞으로 각 동물의 망막연구에 필요한 각 층들을 구별 하는데 좋은 지침이 될 수 있다.
한국재래산양 태아 및 신생아 척수에서 GFAP 면역반응세포에 관한 형태학적 연구
송치원,정수연,이근좌,이강이,이경열,박일권,박미선,정승혁,조규완,김무강,Song, Chi-Won,Chung, Soo-Youn,Lee, Keun-Jwa,Lee, Kang-Iee,Lee, Kyoung-Youl,Park, Il-Kwon,Park, Mi-Sun,Chung, Seung-Hyuk,Cho, Gyu-Woan,Kim, Moo-Kang 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4
Glial fibrillary acidic protein(GFAP) is one of the intermediate filaments, and used as an astrocyte detection marker. GFAP distribution has been studied on the fetal, neonatal and aged brains. In this study, the GFAP immunoreactive cell localization and distribution in the fetal (30, 45, 60, 90, 105 and 120 days of gestation) and neonate spinal cords of Korean native goat were investigated by immunohistochemistry. Nonpolar radial glial cells initiated to appear at 45 days of gestation. GFAP-immunoreactive processes were extended from central canal to pia matter. Bipolar immumoreactive cells were transformed to monopolar and multipolar immunoreactive cells at 45 days of gestation. Multipolar astrocytes of 60 days of gestation were found within white and gray matters of spinal cord. The number of GFAP-immunoreactive cells were gradually decreased from 90 days of gestation until newborn neonate. The intensity of GFAP immunoreactivity was gradually decreased from 95 days of gestation until newborn neonate. These results suggest that the radial glial cells within the gray and white matters of spinal cord are very fast developed.
감마선 조사에 의한 발생과정 중인 마우스의 소뇌발달에 관한 연구
박일권,이경열,박오성,김성화,이근좌,이강이,민태선,육홍선,변명우,김무강,Park, Il-Kwon,Lee, Kyoung-Youl,Park, O-Sung,Kim, Sung-Hwa,Lee, Guen-Jwa,Lee, Kang-Yi,Min, Tae-Sun,Yook, Hong-Sun,Byun, Myung-Woo,Kim, Moo-Kang 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.2
In order to study about the lobule and layer formation and cell migration of the mouse cerebellum from at the birth to 15th day effected by 2.5, 5 and 10 Gy r-raddiation at the 19th pregnancy. The routine tissue preparation and staining procedure, Immunohistochemical staining method by the several antibody and western brotting method were utilized from the birth to the15th day. The results were as followings. 1. The body and cerebellum weights were more slowly increase of the the 2.5 Gy, 5 Gy and 10 Gy irradiation group compare to the control group, and the health condition of the 2.5 Gy group was a little bad. but the 10 Gy group was more severe and begun to die from the 12th day after birth. 2. The thickness, proliferation and migration of the 2.5, 5 and 10 Gy irradiated external granular cells from the maginal zone to the medullary area forming the molecular layer from the 6th day to the 15th day after birth were thinner, weaker and more slower according to the radiated dosages than the control group in the cresyl violet staining. 3. The proliteration, migration and lobulation of the 5 Gy radiated groups from the first day to the 15th day after birth were more weak, incomplete and irregular shape in the immunostaining with Dab, Cdk5, P35, calbindin and Zebrin antibody. 4. In the western blotting analysis using the Reelin, Dab, Cdk5 and P35 antibody. The Bands were in the 60 KD, 80 KD, 33 KD and 35 KD, and there were no differences between the control and irradiated groups in the molecular band except the Reelin. 5. As a results, the proliferation and migration of the outer granular and purkinje cells, and lobulation of the cerebellum by the several dosaege of the ${\gamma}$-ray radiation were proportionally incomplete according to dosage.
고양이 3종(FPV, FHV, FCV) 불활화 백신의 효과
이성민,윤인중,최환원,이근좌,이경열,김무강,Lee, Sung-min,Yoon, In-joong,Choi, Hwan-won,Lee, Keun-jwa,Lee, Kyoung-youl,Kim, Moo-kang 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.3
This study tested the effect of a trivalent (feline panleukopenia; FPV, feline viral rhinotracheitis; FHV, feline calicivirus infection; FCV) inactivated vaccine in cats. The vaccine was tested for the safety in guinea pigs, mice and cats. Also, it was tested for the efficacy in cats. The vaccine was inoculated to cats at 7~9 and 10~12 weeks of age (conventional schedule) and the serological response to vaccination was assessed and was compared to the unvaccinated group. All cats were bled by jugular venipuncture for FPV, FHV and FCV specific serological test (virus neutralizing antibody, VN) at 7~9, 10~12 and 13~15 weeks. After last bleeding, all cats were inoculated with each virus (FPV : orally $2ml\;10^{7.5}\;TCID_{50}/ml$, FHV : nasally $1ml\;10^{7.0}\;TCID_{50}/ml$ and FCV : nasally $1ml\;10^{7.0}\;TCID_{50}/ml$). The Vaccine verified excellent protective effect in guinea pigs, mice and cats. The VN antibody titers of the unvaccinated group cats against FPV, FHV and FCV were <2~16, on the other hand the vaccinated group cats were $512{\sim}{\geq}4096$, 64~1024 and 64~1024, respectively. When all cats were challenged with virulent viruses, the survival rates of the vaccinated group cats were over 80%, while the survival rates of the unvaccinated group cats were less 20%. The typical clinical signs were not observed in the vaccinated group cats, but the typical clinical signs and histopathological lesions were observed in the unvaccinated group cats. As the result of tests, the VN values obtained in this study appeared to be high enough to protect cats from viral challenges. The trivalent (FPV, FHV, and FCV) inactivated vaccine seemed to be very effective, for prevention of feline viral diseases (FPV, FHV, and FCV).
Apoptosis of Germ Cells after Vasectomy in Rats
최종윤,조성환,류시윤,지영흔,이근좌,손화영,Choi, Jong-yun,Cho, Sung-whan,Ryu, Si-yoon,Jee, Young-heun,Lee, Geun-jwa,Son, Hwa-young The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3
The pathological mechanism of impaired spermatogenesis after vasectomy has not been completely investigated. In this study, we examined pathological changes of the testis and the Fas-Fas ligand (FasL) mediated signaling pathway in apoptotic germ cell death after vasectomy in rats. Ten-weeks old Sprague-Dawley rats were underwent bilateral vasectomy and sacrificed after 1 day, 2 days, 3 days, 5 days, 1 week, 2 weeks, and 4 weeks of surgery and the testes were removed. Histopathological evaluation of spermatogenesis was performed by hematoxylin-eosin and periodic acid-Schiff-hematoxylin staining. To elucidate the pathophysiology of seminiferous tubule damage, terminal dUTP nick end labeling staining, electrophoresis assay of DNA fragmentation, and Western blotting analysis for Fas-FasL were performed. Relative weights of testes were decreased from 5 days after vasectomy. Germ cell degeneration were first found in the spermatogonia and spermatocytes at stages I-VI, and XII-XIV seminiferous tubules. Mean incidence of apoptotic germ cells after vasectomy progressively increased to peak in 5 days, and then gradually decreased to the control levels in 2 weeks after vasectomy. The expression of Fas-FasL reached maximum level at 5 days after vasectomy and then declined. In conclusion, impaired spermatogenesis after vasectomy associated with an increase in germ cell apoptasis, which is partly mediated by the activation of Fas-FasL.