Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

구조물의 강성구조에 따른 파동 전파 특성의 이해(Ⅰ)

조미라,홍창국,김기봉 중앙대학교 건설환경연구소 2001 環境科學硏究 Vol.12 No.2

본 연구에서는 뛰어난 현장 적용성으로 구조물의 비파괴 건전도 평가 방법으로 주목받고 있는 충격반향기법의 신뢰성 향상과 정확도 개선을 위하여 콘크리트 구조물에서 전파되는 탄성파의 전파특성을 연구하였다. 이를 위하여 유한 요소해석기법과 동강성행렬법을 도입하여 수치실험을 수행하였다. 또한 이로써 기존에 보고되고 있는 총격반향기법의 수정이론을 검증하고 구조물의 전단강성구조에 따른 P파와 SH파의 전파 특성 등에 의한 평가방법이 미치는 영향을 평가하였으며 이를 바탕으로 충격반향기법의 실험자료 분석 및 해석에 있어서 보다 신뢰성 있는 결과를 도출할 수 있었다. The propagation of the elastic waves transmitting concrete structures was investigated to improve the reliability of the impact echo test, which is recognized as an excellent nondestructive method for the assessment of structural integrity. The finite element analysis and the analytical method of the dynamic stiffness matrix method were introduced for the investigation of the wave propagation, The study was first dedicated to the verification of the modified impact echo test (Kim, et at.,2001 ; Cho,2001), and then to the effects of factors such as stiffness structure and wane type (P-wave and SH-wave). This research enabled the impact echo test to be more reliable in the interpretation and analysis of the measurements.

방사선 조사 마우스에서 소장움세포의 Apoptosis 발생에 미치는 생약의 효과

김성호,안미라,나승렬,이종환,김재하,조성기,장종식,신동호 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

Apoptosis의 유발을 억제하는 한약제를 파악하여 apoptosis와 관련된 정상적 또는 질병에 대한 연구에 자료를 제공하고자 방사선에 의해 유도된 apoptosis를 지표로 한의학적 처방에서 많이 사용되는 대표적 한약제(24종)의 효과를 검증하였다. 용안육, 산조인, 원지, 인삼, 복령, 목향, 천궁, 백작약, 승마, 시호 및 눈꽃동충하초 투여군에서 apoptosis는 감소되었으며, 이들 생약제는 apoptosis와 관련된 질병의 예방 및 치료에 적용할 수 있을 것이다. 추 후 이들 생약의 작용 기전에 대한 연구가 요구된다. This study was performed to determine the effect of several herbs on radiation-induced apoptosis in jejunal crypt cells. Longyanrou(Euphoris longana), Suanzaoren(Zizyphus vulgaris), Yuanzhi(Polygala tenuifolia), Rensan(Panax ginseng), Fuling(Poria cocos), Muxiang(Saussurea lappa), Chuanxiong(Cnidium officinale), Baishaoyao(Paeonia lactifolia), Shengma(Cimicifuga heracleifolia), Chaihu(Bupleurum falcatum) and Dongchongxiacao(Paecilomyces japonica) reduced the frequency of radiation-induced apoptosis(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Longyanrou, Suanzaoren, Yuanzhi, Rensan, Fuling, Muxiang, Chuanxiong, Baishaoyao, Shengma, Chaihu and Dongchongxiacao might be useful inhibitors of apoptosis, especially since these are relative nontoxic natural products.

체표에 의하여 표시한 기초 대사량의 체중에 의한 환산

김남주,박현녀,이은령,정미라 이화여자대학교 의과대학 1980 梨花醫學誌 Vol.- No.12

We tried to convert the basal metabolic rate (B.M.R) of 493 males and 434 females on the basis of surface area of body who measured at our Department of Physiology 1963~1979 to that on the basis of body weight. 1) Our B.M.R. calculation on the basis of square meter is almost similar to early reports of Dr. Dong Jun Kim. 2) B.M.R. on the basis of body weight shows declining tendency in both males and females from 5 years to 35 years as was on the basis of the body surface area. 3) B.M.R. of the male is higher than that of the female. 4) The B.M.R. on the basis of body weight may be applicable to calculate energy expenditure as well as energy requirement in the daily life.

복부비만 여성 대상 걷기 운동 프로그램의 비만관련 지표 및 심폐체력 개선 효과

이미라,김완수 김천과학대학 2007 김천과학대학 논문집 Vol.33 No.-

The purpose of this study was to investigate the effects of a 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, on obesity-related indices (body weight, body mass index, waist circumference, waist-to-bip ratio, body fat percent, and total body fat) and aerobic fitness in Korean women with abdominal obesity. The subjects were 11 middle-aged women with abdominal obesity (waist circumference ≥80 cm). The intervention consisted of walking for 45-60 min per day at an intensity of 40(50) to 60(65)% of heart rate reserve(HRR) for 3 days per week Subjects of this study had decreases in obesity-related indices overall. Decreases in body weight, waist-to-hip ratio, body mass index, waist circumference, and total body fat were statistically significant. Aerobic fitness was increased after the intervention and this increase was close to statistical significance. A 12-wk brisk walking program consisted of the exercise amount, which is comparable with the current minimum physical activity recommendation associated with health benefits, could be an effective strategy to reduce obesity-related indices in middle-aged Korean women with abdominal obesity. Whether higher amount of exercise is required to promote significant improvement in aerobic fitness in this population needs to be investigated in well-controlled further studies.

The efficacy of bench-step aerobic program in reducing some cardiovascular risk factors in overweight or obese women

이미라,김완수 대구대학교 인문과학연구소 2008 人文科學硏究 Vol.32 No.-

본 연구의 목적은 12주 스텝운동 프로그램이 과체중(23 ㎏/㎡≤체질량지수<25 ㎏/㎡) 및 비만(체질량지수≥25 ㎏/㎡) 여성의 심혈관 위험 요인(비만관련 지표와 혈당 및 지질성분)에 미치는 효과를 알아보기 위한 것이다. 선발된 25명의 과체중 및 비만 여성 중 프로그램에 90% 이상 참여하고 프로그램 전과 후에 이루어진 검사를 수행한 15명의 프로그램 수행 전과 후의 비만관련 지표(체중, 체질량지수, 체지방율 및 총체지방), 혈당, 중성지방 그리고 고밀도지단백콜레스테롤을 비교하였다. 본 프로그램은 주 3회, 12주 동안 이루어졌고, 45분에서 60분 동안 예비심박수의 40/50%에서 50/60%의 운동 강도로 진행되었다. 운동량은 건강상의 이점 획득 목적으로 현재 권고되는 최소 운동량에 근거하여 결정되었다. 12주 스텝운동 프로그램 후 대상자의 체지방율이 유의수준에 가깝게 감소되었고(T=2.121, P=.052), 체중, 체질량지수와 체지방이 유의하게 감소되어(T=5.270, P=.000; T=2.658, P=.019; T=3.661, P=.003), 전반적으로 비만관련 지표에 미치는 효과가 지지되었다. 혈당(T=1.850, P=.087), 중성지방(T=-1.249, P=.234) 및 고밀도지단백콜레스테롤(T=.084, P=.934)은 유의한 변화를 보이지 않았다. 본 연구결과는 선행 연구결과들과 함께 12주 스텝운동 프로그램이 전반적으로 비만관련 지표를 유의하게 감소시킴으로써 과체중 및 비만 여성의 심혈관 질환 위험을 감소시킬 수 있는 효과적인 운동 프로그램이 될 수 있음을 보여주고 있다. 반면 혈당과 혈중 지질성분 개선을 위해서는 운동량의 증가 또는 근량 증가 및 내장지방 감소 효과를 초래할 수 있는 운동 프로그램으로의 수정 등과 같은 전략들이 부가되어야 한다는 것을 시사하고 있다. 그러나 본 연구 포함 스텝운동 프로그램의 심혈관 위험 감소 효과를 검증한 현재까지의 연구들은 연구 설계상의 제한점을 갖고 있고, 따라서 일반화를 위한 무작위화되고 통제된 연구가 필요하다.