Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

HSC 기둥의 내진성능에 대한 횡보강근의 효과

한병찬,황선경,성수용,정수영 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

In seismic-resistant design of buildings of ductile frame, the possibility of formation of plastic hinge at column end demands sufficient member ductility. The paper summarizes results of an experimental study of reinforced concrete columns with high-strength materials and subjected to simulated seismic loadings. The experimental program consisted of 6, approximately one-third-scale, rectilinearly confined, high-strength reinforced concrete columns with concrete compressive strengths of approximately 700kgf/cm2. The primary variables of the testing program were the volumetric ratio, spacing, and configuration of transverse reinforcement. Experimental results indicate as follows : 1.There is no expectation of improved effectiveness of deformability by the types of transverse reinforcement when using high yielding strength transverse reinforcement. 2.When using high yielding strength transverse reinforcement, it is possible to widen the spacing that are designed based on seismic provision of ACI 318-89 and get an appropriate deformability as well. 3.The significant parameter to determine the deformability is the spacing of transverse reinforcement in the HSC columns.

고지방식을 섭취한 랫드에서 김치유산균의 콜레스테롤 저하효과

조미선, 노정미, 임형준, 한선경, 신명수, 이완규 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

전통음식에 대한 경북지역 주부들의 의식 및 실태조사

한재숙,한경필,성선향,조연숙,박경숙,김현옥,정종기 동아시아식생활학회 2000 동아시아식생활학회지 Vol.10 No.6

The purpose of this study was to survey the awareness and use for traditional foods of housewives in Kyong-buk area. The results were as follows : Residental areas were city(43.9%) and the county(56.1%). Region were divided as the north resources circle(28.3%), the east coast circle (21.0%), the middle west circle (21.7%), and the south city circle (29.0%). The performance degree of times and seasons of the year customs was Seolnal, Chuseuk, Daeboreum and Dongji in order. Preparation foods of Seolnal were deukguk, vegetablejeon, fishjeon, namul and gangjeung in order. Preparation foods of Chaseuk were songpeon, vegetablejeon, namul, fishjeon and fruits in order. The country foods were sikhae, muk, pumpkin, golbangiguk, yakga and potato in order.

원주의 향토 음식 발굴 및 인지도에 관한 연구 : 원주시민과 조리인을 대상으로 Comparison between Residents and Cooks

한경선,송병춘 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.4

The purpose of this research was to have a proper understanding, to have a concern about native local foods and to discover unknown them in Wonju. The data was obtained by use of questionaries to residents(1977 persons) and cook(172 persons) in Wonju and statistical analysis was performed . Frequency and percentage were acquired using SPSS program. To turn out the signisicant difference between general conditions and each groups t-test and F-test were used. The results of this survey had briefly summarized as follows 1. The results were total 99 kinds of food revealed as a native local foods and 9 kinds of the special products of Wonju. 2. The main materials were potato, com, buckwheal, acom, pheasant, mushroom, arrowroot, and wild edible greens. 3. The local foods in Wonju were were formed with it's own natural material and cooked by original cooking method. 4. The highest perceptional score items were the hot Tang(Hwangtaehaejangguk, chueotang, Oritang, Gamjatang, Minmulmaeuntang, Tojongdakbaeksuk, Hwanggibaeksuk, Eomnamusamgyetang), and the Bap(Bibimbap, Ssambap, Sanchae-jeongsik) and the noodles(Potato sujebi, Dongchimimakhukso, Olchaengiguksu, Jangsujebi, arrowroot noodle,Gamjaongsimi)

항암제 AG60이 흰생쥐 위점막 상피세포의 DNA합성에 미치는 영향 : 자기방사법적연구

박경호,안의태,고정식,하상선,한영복,정영신,홍은경,유보림,김상건,이경영,강종구 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

철근콘크리트 연속보에서 부분프리스트레스 도입에 의한 휨보강 효과에 관한 연구

한병찬,윤현도,박완신,황선경,임재형,문정호 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

A strengthening technique for reinforced concrete beams using external unbonded reinforcement offers advantages in speed and simplicity of installation over other, established, strengthening techniques. The purpose of this paper is to investigate the capabilities of a new retrofitting technique, namely external prestressing(Bow), for flexural strengthening of beams. The paper provides a general description of structural behavior of beams strengthened using the technique. Results of 2 physical tests on strengthened reinforced concrete continuous beams are reported and compared. It is shown that the technique can provide greater strength enhancement to lightly reinforced sections and that provision of deflectors enhances efficiency.

혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례

선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

가와사끼 질환에서 감마글로불린의 대량 정주 용법의 효과에 관한 연구

이경희,하태선,박범수,한헌석,하성훈 충북대학교 의과대학 충북대학교 의학연구소 1994 忠北醫大學術誌 Vol.4 No.1

가와사끼병으로 진단받고 고용량의 감마글로불린(2 gm/㎏)을 정맥주사 받은 17명의 환아를 대상으로 임상적 효과와 관상동맥의 변화에 미치는 영향을 연구하여 다음의 결과를 얻었다. 1. 대상 환아의 연령은 평균 2.03세로 3개월에서 5세 사이에 분포하였고, 2세 이하가 58.8%를 차지하고 남여의 비는 1.125 : 1이었다. 2. 가와사끼 진단 기준의 6가지 주증상 중 5가지는 전환아에서 나타났으며, 경부 림프선 종창만 64.7%에서 나타났다. 발생 계절은 봄과 겨울에 76.5%의 환아가 발생하였다. 3. 대량의 감마글로불린 주사 직후 평균 11.4시간에 고열이 없어지고 다른 주증상들도 없어지기 시작하였으며, 대부분(82.3%)이 주사후 20시간 이내에는 소실되었다. 주사중 3례(17.6%)에서 오한 및 일시적인 체온의 상승을 보였으나 주사를 중단할 필요는 없었다. 4. 발병 4주 이내에 관상동맥의 확장이 관찰된 경우는 8례(47.1%)였으나, 이들은 모두 경도의 확장이었다. 발병 2개월 후에 시행한 심초음파도에서는 2명(11.7%)에서만 경도의 확장을 보였고, 나머지는 모두 정상으로 회복되어 있었다. 5. 최저 헤모글로빈치, 최대 C-반응성 단백질, 최대 총백혈구수, 최고 적혈구 혈침속도, 혈소판 최고치는, 발병 4주 이내와 2개월 후에 관상 동맥의 변화가 있는 군과 정상인 군 사이에 의미 있는 차이는 없었다. 이상의 결과로 가와사끼병의 치료에 있어서 단일 고용량의 감마글로불린을 정맥주사하는 방법이 심각한 부작용 없이 임상적 증세를 빨리 호전시키며, 관상동맥의 합병증을 줄이는데 효과적임을 알 수 있었다. 따라서 모든 가와사끼병의 급성기에 가능한 빨리 단일 고용량의 감마글로불린을 정맥주사하는 것을 추천한다. The authors studied the effect of single high-dose intravenous gammaglobulin(2 gm/㎏) on the clinical symptoms and coronary artery abnormalities in seventeen Kawasaki patients and obtained the following results. 1. The patients' ages ranged from 3-month to 5-year, with mean age of 2.03-year. The patients under 2-years comprised 58.8%. The ratio of male to female was 1.125. 2. All of the patients showed 6 major diagnostic criteria with one exception of cervical lymphadenopathy(64.7%). The disease occurred mainly spring and winter season(76.5%). 3. Fever subsided within 20 hours' of intravenous gammaglobulin infusion in most of the patients(82.3%) with mean value of 11.4 hours, Transient side effects such as fever and chilling sense, developed in 3 cases(17.6%), but there was no need to quit infusion. 4. Echocardiographically, mild coronary arterial dilation was observed in 8 cases(47.1%) within 4 weeks' of onset. After 2 months' of onset, only 2 cases(11.7%) showed mild dilation. 5. There were no significant difference between coronary arterial dilation group and normal group before 4wk and after 2 mo of onset, in terms of hemoglobin nadir, maximal CRP, maximal WBC count, peak ESR, and peak platelet count. Conclusively, single high dose intravenous gammaglobulin infusion in Kawasaki disease showed effective improvement of clinical symptoms and decrease of coronary arterial abnormality. There fore all the children with Kawasaki disease should be treated with single high-dose intravenous gammaglobulin infusion as soon as possible.

지방산 유지 원료에 따른 고형비누의 특성에 관한 연구

정명선,배한수,이봉연,민경혜,류덕환 계명대학교 생활과학연구소 2001 科學論集 Vol.27 No.-

The experimental results on the washing efficiency of detergent made from corn oil, wasted cooking oil and pig fat oil are as follows ; Laundry factors in this experiment are detergent concentration, laundry temperature and laundry time. 1.The surface tension of each detergent showed the lowest in 0.2% concentration corn oil 60.84, wasted cooking oil 61.11, pig fat oil 60.73dyne/cm. 2.The wasthing efficiency based on different concentration of detergents showed the highest in 0.2% concentration. 3.The highest washing efficiency appeared in 0.2% concentration 40℃ temperature and 40~50 min. time. There showed no difference in the washing efficiency according to the volume of water. It can be said that the frictional force of bearing rather than the volume of water worked on the efficiency.