노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

飮食物쓰레기의 效率的 管理를 위한 物理的 特性 및 處理

권효정,정영헌,김임경,심언봉,오광중 부산대학교 환경문제연구소 2003 環境硏究報 Vol.21 No.-

This study was conducted to manage food wastes in Korea efficiently. We carried out an experiments on food wastes generated from Yeonjae gu, Pusan as a representative boundary according to the measurement of apparent density, generation quantity, physical characteristics, proximate analysis, and elemental analysis as well as drying and carbonizing, and also tested the hazardous materials in fertilizer and compost from public and private recycling center in operation. The purposes of this study are to investigate current status of food wastes recycling methods and facilities, to examine the fuelization of food wastes, and to suggest the cost effective food wastes treatment plans. Based on the results of the analysis of apparent density and water content in food wastes, it was revealed that the apparent density of food wastes was in inverse proportion to the water content. The water content of food wastes was highly influenced by the fruit and vegetables such as watermelons. These results indicate that the food wastes with about 75 ~80wt% initial moisture content should be reduced under 70wt% moisture content by drying process pier to throwing away. Comparing the heating values of the food wastes which were calculated by Dulong's equation and Steuer's equation it was revealed that Steuer's equation(HHV 5,186 kcal/kg, LHV 880 kcal/kg) was more compatible than Dulong's equation(HHV 4,676 kcal/kg, LHV 330 kcal/kg) because the results of proximate analysis for the food wastes were very similar to those of sewage sludges. Additionally, to reduce water contents of food wastes under 60wt% as acceptable water contents for fuelization, it was also found that blending with 25wt% chars made from food wastes was more efficient than the drying of food wastes itself at 105°C, 45minutes in view of completion time. Furthermore, these chars generated from food wastes (about LHV 6,608 kcal/kg) comparable to commercial charcoal(LHV 7,134 kcal/kg) generated from oak trees and lower ash contents(under 15wt% of chars) than those.

고온을 받은 콘크리트의 공학적 특성

권영진,김용로,장재봉,김무한 한국화재소방학회 2004 한국화재소방학회논문지 Vol.18 No.1

본 연구는 잔골재 및 혼화재 종류, W/C에 따른 콘크리트의 폭열성상 및 압축강도 초음파속도 등의 공학적 특성을 검토함으로서 폭열 방지 및 화재 피해를 입은 콘크리트 구조물의 재사용과 보수보강, 안전도평가 등에 기초적인 자료를 제시하고자 한다. 폭열성상을 살펴보면, 잔골재에 따라서는 바다모래를 사용한 경우 폭열이 발생하지 않았거나 약간의 폭열이 나타나는 정도이나 재생잔골재나 부순모래를 사용한 경우에는 폭열이 심하게 나타났다. 또한 혼화재 종류에 따라서는 대체로 유사한 폭열성상을 나타내었다. 한편, W/C 30.5%의 고강도 영역에서는 대부분 폭열하였으나 W/C 55%의 보통강도 영역에서는 대부분 폭열하지 않았다. 잔존 압축강도는 W/C 55%의 경우 평균 45%로 나타났으며, W/C 30.5%의 경우에는 평균 6fl%로 나타났다. 초음파속도는 잔골재 종류 및 W/C, 가열시간에 따라 다르게 나타났으며, 가열후 3개월이 가열후 1개월보다 악 1.3-8.4%의 초음파속도 회복을 보였다. The purpose of this study is to present data for the reusing, rehabilitation and estimation of safety of RC structure damaged by fire, and for the prevention of explosive spalling by investigation the properties of explosive spalling, compressive strength and ultrasonic pulse velocity according to kinds of fine aggregate, admixture and water-cement ratios. In explosive spalling properties with kinds of aggregate. explosive spalling does not appear or little at surface in the case of used sea sand, but the case (of using recycled sand or crushed sand is worse and worse. Property with the kind of admixture does not appear specially. And high strength concrete with W/C 30.5% was taken spalling, but 55% does not appear. It is found that residual compressive strength after exposed at high temperature showed 45% in W/C 55%, and 64% in W/C 30.5% of its original strength averagely. Ultrasonic pulse velocity is different with kinds of aggregate, W/C, and heating time. When 3 month age after heating ultrasonic pulse velocity is recovered abut 1.3%-8.4% of its 1 month age after heating.

소득작물 전후작지 적응 극단기성 벼 ‘중모 1032’

손영보,이종희,조준현,이지윤,오성환,송유천,박동수,박노봉,김상열,여운상,남민희,권영업 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

허혈성 심질환에 있어서 아데노신 급속 정맥투여후 관동맥압의 변화에 관한 연구

봉종대,오종용,배성한,신원용,김철현,이광희,최태명,현민수,김성구,권영주 순천향의학연구소 1998 Journal of Soonchunhyang Medical Science Vol.4 No.2

Background: Adenosine interacts with A1 receptors present on the extracellular surface of cardiac cells, activating K+channels in a fashion. It produces coronary vasodilatation and AV nodal block. The half-time is one to six second and has ultra-short action. In ischemic heart disease, fractional flow reserve(FFR) can be achieved safely with intravenous adenosine infusions at a rate of 150㎍/kg/min which cause maximal coronary hyperemia. Purpose: We investigated the patients who have had ischemic heart disease(n=8). After adenosine intravenous administration(150㎍/kg/min), the effect cause maximal hyperemia of coronary artery. During peak maximal hyperemia, we reported clinical findings, coronary hemodynamics and electrocardiogram findings. Methods: After diagnostic coronary angiography and left ventriculography, catheter was advanced into the ostium of coronary artery. We investigated coronary hemodynamics such as systolic coronary artery pressure, diastolic coronary artery pressure, mean coronary artery pressure and heart rate. To assess the use of adenosine for determination of fractional flow reserve, hemodynamics and electrocardiogram were measured at basal state and during peak maximal hyperemia. Results: Symptoms developed 1.5-2 minute after adenosine intravenous administration and each symptom disappeared 2-2.5 minute after adenosine administration. Two patients represented asymptomatic appearance and six patients complained of flushing, chest pain, palpitation and headache. In eight patients with ischemic heart disease, 1.5 minute after venous administration of 150㎍/kg/min of adenosine, coronary artery pressure decreased and heart rate increased significantly. Conduction abnormalities after administration of 150㎍/kg/min of adenosine were transient 2nd degree AV block 2 cases(25%). Conclusions: Adenosine is a potent ultra short-acting vasodilator. Although coronary artery pressure decreased and heart rate increased and mild cardiovascular symptoms occurred after continuous infusion of 150㎍/kg/min of adenosine, it is useful as an agent for determination of fractional flow reserve.

위암발증 원인에 대한 생활습관 및 영양섭취에 있어서 농촌지역 주민 조사 : 강원도 화천군 주민의 Helicobacter pylori 감염률을 중심으로 Infection of Helicobacter pylori in Whachon Area, Kang-Won Do

권태봉,이정선,우영국,이명헌,정철원,주진순 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.1

위암발증의 원인을 밝히기 위하여 설문조사를 통하여 강원도 화천군에 거주하는 주민들의 일반사항, 생활습관 및 영양섭취실태와, ELISA법으로 Helicobacter pylori 감염률을 조사하였다. 조사 대상자는 40∼69세까지의 지역주민 169명으로 남자 79명, 여자 90명이었으며 그 결과는 다음과 같다. 조사 대상자의 학력은 65.7%가 초등학교 이하였으며, 월수입은 60%가 50만원 이하였다. 대상자의 62.7%가 농업에 종사하였고 자녀수는 64.3%가 3∼5명이었으며 대상자의 34.5%가 흡연을 하고 있었고 68.4%가 음주를 한다고 대답하였다. 대상자의 전체 평균신장은 156.7㎝로 여자는 150.1㎝, 남자는 164.4㎝ 이었다. 평균체중은 61.1㎏으로 여자의 체중은 57㎏, 남자는 65.7㎏이었으며 BMI는 전체 평균 25.5%로 여자는 24.8%, 남자는 20.1%이었다. Helicobacter pylori의 감염률은 전체 조사대상자의 66.3%이었으며, 여자의 67.7%, 남자의 64.6%가 감염되었고 연령별로는 40대의 70%, 50대의 62.2%, 60대의 69.4%가 각각 감염된 것으로 나타났다. 일상 생활 요인에 따른 Helicobacter pylori의 감염률은 흡연의 정도가 심하고 학력이 낮으며 월수입이 낮을수록 높은 경향을 보였다. Helicobacter pylori의 감염여부에 따라 감염자와 비감염자의 영양섭취량을 조사해 본 결과 비감염자는 감염자와 비교해서 에너지, 총단백질, 동물성 단백질, 칼슘, 비타민 C 섭취량이 높은 경향을 보였으며 철분과 niacin의 섭취량은 낮은 경향을 보였다. To examine the relationship between the casual infection of gastric cancer and lifestyle and nutritional status, we surveyed 169 persons, 90 females and 79 males, aged 40∼69 years from June 7 to June 14, 1997, in Whachon area, Kang-Won Do, Korea. For this purpose, we investigated Helicobacter pylori infection and identified the effect of sex, age, smoking, alcohol consumption, economic status, occupation, life-style and food intake on Helicobacter pylori infection. Helicobacter pylori status was evaluated using an enzyme-linked immunosorbant assay(ELISA) for anti-Helicobacter pylori immunoglobulin G(IgG). Helicobacter pylori infection was present in 66.3% of total subjects, namely, 67.7% of female and 64.6% of male and its prevalence increased with smoking, low education level and low monthly income. Energy, total protein, calcium and vitamin C intakes in the negative Helicobacter pylori infection group were higher than those in positive Helicobacter pylori infection group. However iron and niacin intakes were lower in the Helicobacter pylori negative group than in the positive group.

화재피해를 입은 콘크리트의 공학적 특성에 관한 실험적 연구

권영진,김무한,김용로,장재봉 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.1

A fife outbreak in a reinforcement concrete structure looses the organism by the different contraction and expansion of hardened cement pastes and aggregate, and cause" cracks by thermal stress, leading to the deterioration of the durability So concrete reinforcement structure is damaged partial or whole structure system Therefore accurate diagnosis of deterioration is needed based on mechanism of fire deterioration In general concrete structures Fundamental information and data on the properties of concrete exposed to high temperature are necessary for accurate diagnosis of deterioration In this study, normal and high strength concrete were exposed to a high temperature environment And than fundamental data for the character change of concrete heated highly were presented by measuring characters of explosive spalling, ultrasonic pulse velocity and compressive strength before and after heating It is intended to present data for the exact diagnosis and selection of rep31r and reinforcement system of the deteriorated concrete heated highly

개 大腦皮質의 ATPase에 關한 速度論的 硏究

權奉圭,朴暎淳 圓光大學校 1984 論文集 Vol.18 No.2

本硏究에서는 개 大腦皮質의 小胞體에 結合된 ATPase의 一般的 特性과 detergents의 效果 및 tetracaine의 作用機能에 對하여 硏究하였다. 1. ?? 活性에 미치는 detergents의 影響은 高濃度(0.125~2%)에서 Lubrol=WX) trton X-100 > Sodium deoxycholate > digitonin의 順이었다. 그러나 非 이온性 detergents와는 다르게 低濃度(0.01-0.1%)의 Sodium deoxycholate는 ATPase 活性을 增加시켜 주었으며 ??의 順이었다. 이는 各 ATPaserk 서로 다른 狀態로 小胞體膜에 結合하고 있다는 것을 보여 주는 것이다. 2. 4mM MgCl₂ 濃度下에서 ?? 最適濃度는 0.1 mM 이었으나 MgCl₂가 不在일때는 ??의 最適濃度는 變化를 일으켰다. 만일 小胞體를 -10℃와 Sodium deoxycholate로 處理하면 MgCl?의 最適濃度는 變化를 일으키는 것으로 보아 溫度와 detergents는 PTPase의 蛋白質 構造를 變形시키는 것으로 보이며 tetracaine으로 處理하면 ??의 最適濃度는 0.1 mM에서 0.15 mM로 變化시켜 주는 것으로 보아 ?? 依存性 ??는 tetracaine에 의하여 分子水準에서 變形이 일어나 ??의 吸收와 放出을 妨害시켜 活動電位의 역치가 상승되는 것으로 생각되어 진다. Arrhenis plots에서 형성되는 切線은 이를 뒷 바침 해 준다.

평판구조물의 좌굴거동 추적을 위한 새로운 해석방법의 개발

권영봉 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.1

본 연구의 목적은 박판구조물의 좌굴 및 후좌굴의 거동을 추적하기 위한 비선형유한대판법 프로그램의 개발에 있으며 형상함수로는 판요소의 횡방향은 다항식을 종방향으로는 종형함수를 사용하였다. 전통적인 선형좌굴해석에서는 고려할 수 없는 기하학적인 초기부정형과 잔류응력 및 이의 영향으로 좌굴을 일으키기 전에 발생되는 휨변형을 고려할 수 있는 비선형좌굴 해석기법으로서 계산능률 및 정확도를 향상시키기 위하여 대수 및 수치적분을 혼합하여 사용하였으며 기존의 유한대판법과는 달리 다양한 단부구속조건 및 하중에 제한없이 적용이 가능하다. 초기부정형을 가진 평판과 평판요소로 구성된 박판단면에 적용하여 기존의 결과들과 비교하여 적용가능성을 평가하고 좌굴의 초기부정형에 대한 민감도를 연구하였다. The purpose of this paper is to provide and verify an analytical method, based on the spline finite strip method, which can be used to investigate the buckling mode and stress of thin-walled structures. Geometric imperfection and initial stress of plate and plate assemblies, which are resulted from various preloadings and may cause prebuckling deformations before buckling, are included in the analysis, Both numerical and algebraic integrations have been performed for matrix computations to improve the versatility so that the stress field originated from the initial and prebuckling deformations can be included. It can be applied to sections with simple or non-simple boundary conditions and subjected to arbitrary loading. The method has been applied to investigate the buckling behavior of plates and plate assemblies subjected to compression with initial imperfections and residual stresses. The sensitivity of the buckling behavior to the initial deformations are also studied.

신경회로망 제어기를 이용한 PID 파라미터 추정에 관한 연구

權重東,裵銀敬,金恩基,全基英,李承桓,吳鳳煥,李勳九,金容珠,韓慶熙 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

In this paper, supposed to solve these problem to PID parameters controller algorithm using ANN. In the proposed algorithm, the parameters of the controller were adjusted to reduce by on-line system the error of the speed of IM. In this process, EBPA NN was constituted to an output error value of an IM and conspired an input and output. The performance of the self-tuning controller is compared with that of the PH) controller tuned by conventional method (Ziehler-Nichols). The effectiveness of the proposed control method is verified thought the Matlab Simulink and experimental results.