공황장애의 임상적 특성

고은정,최영희,박기환,이정흠 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.2

Objectives : To clarify clinical features of panic disorder and to compare the characteristics of cognitive patterns, avoidance behaviors, and coping strategies among patients who have panic disorder with or without agoraphobia. Methods : Family backgrounds, cognitive patterns in the first panic attack, coping strategies, and avoidance behaviors were compared among the three groups classified by panic patients with mild agoraphobia(mild PDA=66), severe agoraphobia(severe PDA=71), and without agoraphobia(PD=21) Results : 1) Severe PDA reported significantly high panic attack frequency and more dysfunctional level than PD. 2) Catastrophic thinking of 'dying' in PD was significantly frequent than the other groups. Other fears of 'going craze or loss of control' in severe PDA were the highest among the three groups, though it was not significant. Specific thinking process or interpretation in the first panic attack was not significantly different among the three groups. 3) As avoidance behaviors became worse, the frequency of avoidance behaviors and the patters of avoidance behaviors increased. Conclusions : The results suggested that as avoidance behaviors became worse, the frequency of panic, maladaptive functioning and the patterns of avoidance behaviors increase. This might be why we need to decrease avoidance behaviors through exposure as the treatment.

만성 C형 간염 환자에서 페그인터페론 알파2a와 리바비린 병합 치료중 발생한 벨마비 1예

김일환,장제혁,유충헌,최규남,고정해,김윤정,서광원,김지현,박성재,박은택,이연재,이상혁,설상영 인제대학교 2008 仁濟醫學 Vol.29 No.-

페그인터페론과 리바비린 병합요법은 만성 C형 간염의 일차 치료법이다. 저자들은 만성 C형 간염 환자에서 페그인터페론 과 리바비린 병합 요법 중에 발생한 벨마비 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는 5년 전부터 만성 C형 간염을 앓아온 48세 남자이며, PEG-IFN α-2a 135μgm 피하주사 주1회와 하루 1200㎎의 리바비린을 투여하였다. 치료시작 후 9개월째 환자는 오른쪽 안면의 근력약화를 호소하였으며 벨마비로 진단되었다. 페그인터페론과 리바비린 병합요법을 지속하면서 관찰하였다. 환자의 벨마비는 페그인터페론 치료를 중단하지 않았음에도 3개월후 증상이 회복되고 이후 벨마비 재발 없이 현재 경과관찰 중이다. 만성 C형 간염에서 페그인터페론과 리바비린 병합 요법시 벨마비의 발생 가능성을 염두에 두어야 하겠다. A Case of Bell's Palsy Associated with Combination Therapy of Pegylated Interferon Alfa-2a (PEG-IFN) and Ribavirin for Chronic Hepatitis C Virus Infection Pegylated interferon alfa(PEG-IFN α) and ribavirin therapy is the first line treatment for chronic hepatitis C. Mild complications of the therapy are common, but more serious complications are rare. We report here a case of Bell's palsy that occurred in a patient with chronic hepatitis C virus infection during combination therapy of PEG-IFN α-2a and ribavirin. The patient was 49-year-old man with chronic hepatitis C (genotype 1b) for 8 years. He had compensated liver cirrhosis with splenomegaly. Therapy with PEG-IFN α- 2a 135mcg/week and ribavirin 1200mg/day was initiated. After 9 months of the therapy, the patient showed a loss of muscular tone on the right side of his face. A diagnosis of Bell's palsy was made. The Bell's palsy resolved over 3 months despite continuation of the combination therapy.

보신 관광에서 감염된 수입성 Sparganosis 1예 : Intake of Thailand Snake during Trip

서환조,박명재,고경식,백영한,조유정,양현종,류경남,정형근,전정열 대한감염학회 1992 감염 Vol.24 No.1

Human sparganosois was reported more than 100 cases in Korean population. However no case reported yet in Korea as a patient was infected by sparganum from other geographical area, such as Thailand. The case we are reported here is a 38 year-old Korean male who had been suffered from mass being a small, round and soft consistance in upper portion of his right thigh. The patients stated that he had an opportunity to eat living snake in Bangkok, Thailand during trip. As therapy praziquantel (75㎎/㎏/day) was given p.o. for 14 days, but in vain to cure. Following medical treatment, surgical exicision was performed to remove a alive, soft, whitish-milky, mobile and elongated sparganum. Serlogical test by micro-ELISA for sparganum-specific antibody revealed a high serum antibody level of absorbance 0.67 (positive criterion: absorbance 0.22).

Nocardia orientalis 변이주에 의한 고농도 혼합당을 이용한 반코마이신 생산

김창호,고영환,고중환 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

반코마이신 생성에 미치는 탄소원의 영향을 조사하였다. 조사한 탄소원중 포도당, 말토스, 과당이 반코마이신의 생성에 양호하였으나 포도당 7.5% 이상의 농도에서는 반코마이신의 생성이 억제되었다. 그러나 말토스는 20%까지 반코마이신의 생성을 억제하지 않았다. 말토스와 포도당을 혼합하여 배양한 결과 3 : 1 내지 4 : 1 비율로 하였을 때 가장 양호한 결과를 나타내었다. 포도당 농도를 3%로 고정후 말토스를 첨가하여 발효조에 의한 배양을 실시한 결과 당농도가 16%까지 catabolite repression 현상을 보이지 않고 반코마이신 생성이 용이하였다. 상업적으로 가격이 싼 탄소원을 이용하기 위하여 포도당, 말토스 및 다당류(말토트리오스아 말토테트로스)를 함유하고 이는 가수분해 전분을 이용하여 반코마이신 발효를 실시하였다. 배지내 초기 포도당 농도를 3.0%로 맞춘 후 배양중 말토스 이상의 다당류들이 균체에 의하여 이용되는 과정을 알아보기 위하여 발효액 상등액을 HPLC를 이용하여 시간에 따른 각 당들의 농도 변화를 살펴보았다. 배양 시작 후 38시간동안 포도당이 단독으로 소모되면서 균체의 성장이 빠르게 일어났다. 균체의 성장이 멈춘 후 말토트리오스 및 말토테트로스가 분해되면서 말토스와 포도당의 농도가 증가되었고 이때 포도당의 농도는 증가하여 3.0% 이내로 유지되면서 반코마이신의 생성이 계속되었다. The effects of carbon sources on vancomycin production were investigated using Nocardia orientalis CSVC 3300. Among carbon sources tested, glucose, maltose and fructose were effective for the production of vancomycin. Glucose was favored for growth, but decrease the production of vancomycin at the concentration above 7.5%. In comparison, maltose did not decrease the production of vancomycin up to the concentration of 20%. When the mixture of glucose and maltose was used in the ratio 1:3 to 1:4, the highest production of vancomycin was achieved. When glucose concentration was set at 3.0%, catabolite repression could not be observed up to total sugar concentration of 16.0%. Fermentation was carried out using commercial hydrolyzed starch composed of glucose, maltose, maltotriose and maltotetraose. The initial glucose concentration was set at 3.0% and subsequent oligosaccharide consumption was monitored by checking their supernatant with HPLC. During initial cultivation for 38 hour, glucose was the sole carbon source leading to rapid growth. After cell growth stopped, the maltose and glucose concentrations increased due to degradation of maltotriose and maltotetraose, but glucose level was maintained at around 3.0%. After 70 hour fermentation, maltose slowly converted to glucose, and vancomycin production continued during the period.

미숙아에서 위식도 역류와 무호흡간의 관계

고재성,김재영,박경란,김병일,최중환,서정기 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.6

인체 유방암종에서 Cyclic-Dependent Kinase Inhibitor, p27^Kip1 발현의 의의

송상용,김덕환,서연림,고영혜,김대식,남석진,양정현 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

Dietary Calcium and 1,25-Dihydroxyvitamin D₃ Regulate Transcription of Calcium Transporter Genes in Calbindin-D9k Knockout Mice

KO, Sang-Hwan,LEE, Geun-Shik,VO, Thuy T. B.,JUNG, Eui-Man,CHOI, Kyung-Chul,CHEUNG, Ki-Wha,KIM, Jae Wha,PARK, Jong-Gil,OH, Goo Taeg,JEUNG, Eui-Bae Society for Reproduction and Development 2009 Journal of Reproduction and Development Vol.55 No.2

<P>The effect(s) of oral calcium and vitamin D<SUB>3</SUB> were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (<I>CaBP-9k</I>) and calbindin-D28k (<I>CaBP-28k</I>), transient receptor potential cation channels (<I>TRPV5</I> and <I>TRPV6</I>), Na<SUP>+</SUP>/Ca<SUP>2+</SUP> exchanger 1 (<I>NCX1</I>) and plasma membrane calcium ATPase 1b (<I>PMCA1b</I>), in <I>CaBP-9k</I> KO mice. Wild-type (WT) and KO mice were provided with calcium and vitamin D<SUB>3</SUB>-deficient diets for 10 weeks. The deficient diet significantly decreased body weights compared with the normal diet groups. The serum calcium concentration of the WT mice was decreased by the deficient diet but was unchanged in the KO mice. The deficient diet significantly increased duodenal transcription of <I>CaBP-9k</I> and <I>TRPV6</I> in the WT mice, but no alteration was observed in the KO mice. In the kidney, the deficient diet significantly increased renal transcripts of <I>CaBP-9k</I>, <I>TRPV6</I>, <I>PMCA1b</I>, <I>CaBP-28k</I> and <I>TRPV5</I> in the WT mice but did not alter calcium-relating genes in the KO mice. Two potential mediators of calcium-processing genes, vitamin D receptor (VDR) and parathyroid hormone receptor (PTHR), have been suggested to be useful for elucidating these differential regulations in the calcium-related genes of the KO mice. Expression of VDR was not significantly affected by diet or the KO mutation. Renal <I>PTHR</I> mRNA levels were reduced by the diet, and reduced expression was also seen in the KO mice given the normal diet. Taken together, these results suggest that the active calcium transporting genes in KO mice may have resistance to the deficiency diet of calcium and vitamin D<SUB>3</SUB>.</P>

Effective Reconstruction of Stereoscopic Image Pair by using Regularized Adaptive Window Matching Algorithm

Ko, Jung-Hwan,Lee, Sang-Tae,Kim, Eun-Soo The Korean Infomation Display Society 2004 Journal of information display Vol.5 No.4

In this paper, an effective method for reconstruction of stereoscopic image pair through the regularized adaptive disparity estimation is proposed. Although the conventional adaptive disparity window matching can sharply improve the PSNR of a reconstructed stereo image, but there still exist some problems of overlapping between the matching windows and disallocation of the matching windows, because the size of the matching window tend to changes adaptively in accordance with the magnitude of the feature values. In the proposed method, the problems relating to the conventional adaptive disparity estimation scheme can be solved and the predicted stereo image can be more effectively reconstructed by regularizing the extimated disparity vector with the neighboring disparity vectors. From the experimental results, it is found that the proposed algorithm show improvements the PSNR of the reconstructed right image by about 2.36${\sim}$2.76 dB, on average, compared with that of conventional algorithms.

In vitro Derby Imaging of Cancer Biomarkers Using Quantum Dots

Ko, Mee Hyang,Kim, Soonhag,Kang, Won Jun,Lee, Jung Hwan,Kang, Hyungu,Moon, Sung Hwan,Hwang, Do Won,Ko, Hae Young,Lee, Dong Soo WILEY-VCH Verlag 2009 Small Vol.5 No.10

<P>Semiconductor quantum dots (QDs), which have broad absorption with narrow emission spectra, are useful for multiplex imaging. Here, fluorescence derby imaging using dual color QDs conjugated by the AS1411 aptamer (targeting nucleolin) and the arginine–glycine–aspartic acid (targeting the integrin α<SUB>v</SUB>β<SUB>3</SUB>) in cancer cells is reported. Simultaneous fluorescence imaging of cellular distribution of nucleolin and integrin α<SUB>v</SUB>β<SUB>3</SUB> using QDs enables easy monitoring of separate targets in the cancer cells and the normal healthy cells. These results suggest the feasibility of a concurrent visualization of QD-based multiple cancer biomarkers using small molecules such as aptamer or peptide ligands.</P> <B>Graphic Abstract</B> <P>In vitro derby imaging of RGD peptide targeting integrin α<SUB>v</SUB>β<SUB>3</SUB> protein (green in image) and AS1411 aptamer targeting nucleolin protein (red) using two different quantum dots (605 and 655 nm) is successfully visualized in HeLa cells, simultaneously. Fluorescent imaging signatures of the overlay (yellow) from the integrin α<SUB>v</SUB>β<SUB>3</SUB> and nucleolin show the co-localization in the cellular membrane. <img src='wiley_img/16136810-2009-5-10-SMLL200801580-content.gif' alt='wiley_img/16136810-2009-5-10-SMLL200801580-content'> </P>

Optimization of in vitro Seed Germination of Taraxacum platycarpum

Jung-Hwan LEE,Youngkwan KIM,Da-Young PARK,Yangkang SO,Kuk-Young JUNG,Kisung KO 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10