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      • KCI등재후보

        Effect of Boron on the Development of Adventitious Roots in Sunflower Seedlings

        고은정,홍정희 한국생명과학회 2002 생명과학회지 Vol.12 No.6

        유식물의 부정근 발달에 미치는 붕소의 효과고은정·홍정희(부산대학교 생물학과) Three-day-old sunflower(Helianthus annuus L.) seedlings were de-rooted and incubated in the nutrient solutions that contained either deficient or sufficient boron supply, and exposure to various pH and temperatures. In the absence of boron, no adventitious roots were formed in the majority of the seedlings. Boron caused the development of numerous adventitious roots in the lower part of the hypocotyl. The low pH damage was ameliorated by the simultaneous application of calcium, implying the involvement of calcium-requiring process in overcoming proton toxicity. Borate showed a strong ameliorative effect with Ca2+. These results suggest that the primary target of proton toxicity may be linked to a disturbance of the stability in the pectic polysaccharide network, where calcium plays a key role in plant roots. Maximum temperature for the growth of adventitious roots was 25℃. Although cessation of growth is the most apparent symptom of boron deficiency, exogenous ascorbate improved adventitious root growth in plants in the absence of boron. From the results it is suggested that the inhibition of adventitious root resulting from boron deficiency, low pH and temperature damage may be a consequence of disrupted ascorbate metabolism.Key words - Helianthus annuus, adventitious roots, boron, low pH, calcium, ascorbate

      • KCI등재

        바이오에너지용 수수 품종의 재분화율 증진을 위한 배지와 생장조절제 효과

        고은정,성은수,유지혜,길현영,이재근,황인성,김남준,비말쿠말기미레,김명조,임정대,유창연,이주경,김나영 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 ㎎/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 mg l-1 6-benzyl amino purine (BAP) and 0.25 mg l-1 indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 mg l-1) supplemented with IBA (0.25 mg l-1) was effective combination for shoot multiplication. MS medium supplemented with 1 mg l-1 IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor. 본 연구는 수수의 성숙 종자로부터 캘러스유기 및 재분화 효율 조사하기위하여 수행되었다. 배발생 캘러스 형성을 살펴보기 위해 생장조절제 효과를 조사하였다. 수수 성숙 종자로부터 캘러스 유기에 적합한 생장조절제 종류와 농도는 2 ㎎/L 2,4-D인 것으로 나타났다. 배발생 캘러스의 고효율 빈도는 B5 배지에 2 ㎎/L 2,4-D을 첨가하였을 때 가장 양호한 것으로 나타났다. 배발생 캘러스로부터 식물체 재분화는 BAP와 IBA를 넣은 MS 배지가 가장 적절한 것으로 나타났다. BAP 농도가 높을수록 신초재분화가 활발하였다. 따라서, 본 연구 결과로부터 수수 기내배양시 신초와 발근 형성에 효과적인 생장조절제 조합은 0.5 mg l^(-1) BAP와 0.25 mg l^(-1) IBA인 것으로 나타났다.

      • 공황장애의 임상적 특성

        고은정,최영희,박기환,이정흠 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.2

        Objectives : To clarify clinical features of panic disorder and to compare the characteristics of cognitive patterns, avoidance behaviors, and coping strategies among patients who have panic disorder with or without agoraphobia. Methods : Family backgrounds, cognitive patterns in the first panic attack, coping strategies, and avoidance behaviors were compared among the three groups classified by panic patients with mild agoraphobia(mild PDA=66), severe agoraphobia(severe PDA=71), and without agoraphobia(PD=21) Results : 1) Severe PDA reported significantly high panic attack frequency and more dysfunctional level than PD. 2) Catastrophic thinking of 'dying' in PD was significantly frequent than the other groups. Other fears of 'going craze or loss of control' in severe PDA were the highest among the three groups, though it was not significant. Specific thinking process or interpretation in the first panic attack was not significantly different among the three groups. 3) As avoidance behaviors became worse, the frequency of avoidance behaviors and the patters of avoidance behaviors increased. Conclusions : The results suggested that as avoidance behaviors became worse, the frequency of panic, maladaptive functioning and the patterns of avoidance behaviors increase. This might be why we need to decrease avoidance behaviors through exposure as the treatment.

      • KCI등재

        미국 도입 수수 유전자원의 항산화활성과 항균활성

        고은정,유지혜,성은수,이재근,황인성,김남준,유창연 한국자원식물학회 2012 한국자원식물학회지 Vol.25 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        This study was carried out to evaluate the agronomic characteristics and biological activities of Sorghum bicolor germplasms introduced from USA. Plant height, stem diameter, tiller number, leaf length, leaf width, leaf vein color, ear type, ear length and ear width have different from the cultivated accessions. Sweet-N-Sterile (#4) showed the tallest height,widest ear-width and stem diameter. Most of Sorghum bicolor plants may be available to use for bio-energy from these results showing big biomass. Antioxidant activities of 11 cultivars collected from USA were examined by DPPH assay and reducing power. Among the cultivars, Premium stock (#1), Early Sumac (#7), SS Silage (#9) and WGF Grain Sorghum (#11) showed a significantly higher antioxidant activity in comparison to others. Early Sumac (#7) and SS silage (#9)showed more strong reducing power activities than α-tocopherol, a positive control. Premium stock (#1), Sweet-N-Sterile (#4), Early Sumac (#7) and SS Silage (#9) were also showed high antioxidant activities by DPPH assay and reducing power experiment. BMR Gold I (#3) displayed strong antimicrobial activity against Escherichia coli at minimum inhibitory concentrations (125 μg/ml). 본 연구는 미국 수수 수집종의 작물학적 특성 및 생리활성을 검정하기 위하여 수행되었다. 대부분의 미국 수수 수집종은 200 cm 이상의 큰 초장을 보였으며, 간경 또한 2cm 이상의 값을 나타냈다. 수수 수집종 중에서는 Sweet-N-Sterile(#4)이 가장 우량한 수수의 생장 특성을 보였다. 수수의 DPPH를 이용한 활성 검정 결과, Premium stock (#1), Early Sumac(#7), SS Silage(#9), WGF Grain Sorghum(#11) 4개의 수집종에서 기존의 항산화제인 α-tocopherol과 BHA와 비슷한 활성을 보였고 BHT보다는높은 활성을 보였다. 항미생물 실험결과 BMR Gold I(#3)이 가장 높은 활성을 나타냈으며, 특히 Escherichia coli 에서 125 μg/ml으로 높은 활성수치를 나타냈다.

      • KCI등재후보

        치과위생사의 근무환경에 따른 건강상태와 근골격계 자각증상

        고은정,Go, Eun-jeong 대한치과위생학회한국치위생과학회 2019 대한치위생과학회지 Vol.2 No.1

        To prevent the musculoskeletal diseases from repeated dental treatment, the health status and subjective symptom according to the working environment of dental hygienists were examined in 185 Gyeongnam dental hygienists from October 01, 2017 to October 30. The health status of the study subjects (χ<sup>2</sup>=40.21, p<.001), the physical burden of work (χ<sup>2</sup>=47.68, p<.001) and the mental fatigue of work (χ<sup>2</sup>=66.98, p<.001) were significantly different according to working experience. The level of mental fatigue depended on the number of dental hygienists in the clinics (p=0.032). 94.1% of the subjects knew musculoskeletal diseases and there were significant differences according to working experience (χ<sup>2</sup>=77.85, p<.001), the numbers of patients in a day (χ<sup>2</sup>=41.08, p<.001) and daily standing time (χ<sup>2</sup>=6.96, p=.008). Currently, 73.0% of the dental hygienists have musculoskeletal diseases. There was a significant difference according to the number of patients (χ<sup>2</sup>=51.01, p<.001) and daily standing time (χ<sup>2</sup>=25.15, p<.001). The presence of injured parts due to musculoskeletal disorders showed a significant difference according to the numbers of patients (χ<sup>2</sup>=18.98, p<.001) and daily standing hours (χ<sup>2</sup>=33.20, p<.001). The musculoskeletal diseases examination of dental hygienists is needed based on subjective symptoms of musculoskeletal diseases and prevention and management of measures musculoskeletal diseases are required.

      • KCI등재

        산화실리콘 기판위에 증착된 Bi3.25La0.75Ti3O12 박막의 전기적 특성에 관한 연구

        고은정,박광서,남광우,박재문,심동현 한국물리학회 2004 New Physics: Sae Mulli Vol.48 No.6

        The fabrications and the electrical properties of a ferroelectric-gated eld eect transistor were studied with the use of a thermally oxidized SiO2 dielectric layer prepared on an n-type silicon substrate at an oxidation temperature of 800 C at an O2 pressure of 1 atm. The thicknessed of the lms for ozidation times of 30 and 60 min were measured by using small-angle X-ray scattering and were found to be roughly 8 nm and 15 nm, respectively. Ferroelectric Bi3:25La0:75Ti3O12 (BLT) lms was chosen for use in the MFIS structures due to its large polarization. The capacitance-voltage characteristics for both MIS and MFIS capacitors shifted toward the positive voltage at higher frequencies. From the C-V properties of MFIS capacitor structure, the memory window value was measured to be 4 V, which is sucient for use in ferroelectric-gated eld-eect transistors.

      • KCI등재후보

        Application of Laser Based Spectroscopic Monitoring into Soil Remediation Process of PAH-Contaminated Soil

        고은정,김경웅,곽지현,김주용,박기홍,함세영 한국자원공학회 2011 Geosystem engineering Vol.14 No.1

        The application of Laser-Induced Fluorescence (LIF) into soil remediation process is not popular due to the difficulties of the interpretation of remediation process. The monitoring for the lab-scale surfactant enhanced electrokinetic remediation process using the LIF was performed. The variation of the fluorescence intensity and the change of the fluorescence spectral signature reflecting the nature of the surrounding environment were investigated. The diffuse reflectance correction method for the soil matrix properties on fluorescence, and the time resolution for isolation of surfactant which can be overlapped with PAHs spectra of interest were considered to provide the information of contaminants taking place during the remediation process. Eletrokinetic (EK) remediation process as control experiment was also performed and this result was compared with the surfactant-enhanced EK process especially in terms of the spectral signature of PAHs. The LIF monitoring for soil remediation process showed that the relative quantities and the state of contaminant in soil media which can be an important key to assess the feasibility of remediation process.

      • KCI등재

        The Return of an Old Worm: Cerebral Paragonimiasis Presenting with Intracerebral Hemorrhage

        고은정,김승기,왕규창,채종일,Sangjoon Chong,박성혜,천정은,피지훈 대한의학회 2012 Journal of Korean medical science Vol.27 No.11

        Paragonimiasis is caused by ingesting crustaceans, which are the intermediate hosts of Paragonimus. The involvement of the brain was a common presentation in Korea decades ago, but it becomes much less frequent in domestic medical practices. We observed a rare case of cerebral paragonimiasis manifesting with intracerebral hemorrhage. A 10-yr-old girl presented with sudden-onset dysarthria, right facial palsy and clumsiness of the right hand. Brain imaging showed acute intracerebral hemorrhage in the left frontal area. An occult vascular malformation or small arteriovenous malformation compressed by the hematoma was initially suspected. The lesion progressed for over 2 months until a delayed surgery was undertaken. Pathologic examination was consistent with cerebral paragonimiasis. After chemotherapy with praziquantel, the patient was monitored without neurological deficits or seizure attacks for 6 months. This case alerts practicing clinicians to the domestic transmission of a forgotten parasitic disease due to environmental changes.

      • KCI등재후보

        Surgical Results in Pediatric Patients with Intractable Epilepsy: In Aspects of Development and Cognitive Function

        고은정,은종필,최하영 대한신경외과학회 2004 Journal of Korean neurosurgical society Vol.36 No.1

        Objective : This study is designed to investigate whether early surgery can result in a better outcome for seizure control and brain development in pediatric patients with intractable epilepsy. Methods : Preoperative evaluation methods for surgery included history taking, neurological examination, chronic video-EEG monitoring with surface and subdural grid electrodes, magnetic resonance image(MRI), 3-dimensional surface rendering with MRI, PET and SPECT. The Denver Developmental Screening Test II, and the Full Scale Intelligence Quotient(FSIQ) were evaluated for developmental status before and after surgery. Seizure outcome was classified according to Engel's classification. Surgical procedures included temporal lobectomy in four subjects, extratemporal resection in 14, callosotomy in one and tumor removal in one. Results : Seizure outcomes were class I in 11 patients(55%), class II in three(15%), class III in 4(20%) and class IV in two (10%). Under the age of 6 years, the preoperative average developmental delay was 12.4 months. Postoperatively, two of them caught up to their normal developmental status. Over the age of 6 years, the preoperative average FSIQ was 87.5 and the postoperative average FSIQ was 103.3. Conclusion : In pediatric patients with intractable epilepsy, the early surgical treatment is very helpful in the normalization of their brain function.

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