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Helicobacter pylori와 대장균의 Shuttle Vector 개발
조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-
In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.
Four Year Surveillance of the Vector Hard Ticks for SFTS, Ganghwa-do, Republic of Korea
Myung-Deok Kim-Jeon,Seung Jegal,Hojong Jun,Haneul Jung,Seo Hye Park,Seong Kyu Ahn,Jinyoung Lee,Young Woo Gong,Kwangsig Joo,Mun Ju Kwon,Jong Yul Roh,Wook-Gyo Lee,Young Yil Bahk,Tong-Soo Kim 대한기생충학열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.6
Myung-Deok Kim-Jeon,Song Mi Moon,Sung Suck Oh,Haeyoung Kim,고연자,Seung Jegal,Se Youn Han,Mi Yeon Lee,Young Woo GONG,박윤수 대한미생물학회 2022 Journal of Bacteriology and Virology Vol.52 No.2
The rural areas are the main outbreak sites of severe fever with thrombocytopenia syndrome (SFTS). To identify the seroprevelance of SFTS in rural island, we conducted a serosurveillance study of SFTS virus (SFTSV) of the general public in Mui Island, a representative rural island of Incheon, South Korea. A total of 203 participants (female 127, male 76, 30 to 97 years old, median 67) without symptoms or signs of SFTS were collected via a convenience sampling. Nested reverse transcription PCR was conducted for both the S and M segments of SFTSV gene. Indirect immunofluorescence assay was also performed for immunoglobulin G (IgG) and immunoglobulin M (IgM) against SFTSV. Only 1 participant (0.5%) was positive to SFTSV gene without IgM against SFTSV. In addition, 12 participants (5.9%) were positive to IgG against SFTSV. Seropositive rate of IgG against SFTSV was higher in age group >65 than ≤65 (9.6% vs. 1.1%, OR 9.202; 95% CI, 1.165–72.692; p=0.026) and was not statistically significant according to sex, occupation and duration of residence in Mui Island. This study suggests that SFTSV readily have infected humans in the rural island of Incheon, South Korea. Therefore, reinforced surveillance about SFTS is needed, focusing on medically vulnerable area such as an island.
김완섭,김영석,이혜경,이문성,오명진,심찬섭,전훈배,김영민,임상복,조영덕,송상훈,진소영,문종호,정인섭,허경렬,전진 대한소화기학회 2000 대한소화기학회지 Vol.35 No.4
Neuroendocrine neoplasms of the duodenum constitute 5% of all gastrointestinal carcinoids, but primary ampullary neuroendocrine tumors are very rare. Carcinoid tumors have been described as the most common primary ampullary neuroendocrine tumor. We experienced a 54-year-old man with small cell neuroendocrine carcinoma arising in the ampulla of Vater. Abdominal CT scan revealed dilated common bile duct and enhancing mass at the ampulla of Vater. Duodenoscopic findings showed a huge protruding mass with ulcerative orifice at the ampulla of Vater. Microscopic examination of the tumor revealed small monotonous anaplastic cells. The tumor cells were characterized with hyperchromatic nuclei and high nuclear to cytoplasmic ratio, frequent mitoses. Immunohistochemical staining was strongly positive for neuron specific enolase. We report this case with a review of literatures.
김지명(Kim, Ji-myung),이승현(Lee, Seung-hyun),전용덕(Jeon, Yong-deok),이재섭(Lee, Jae-seob) 대한건축학회 2019 대한건축학회 학술발표대회 논문집 Vol.39 No.1
The purpose of this study is to propose an economic evaluation model of exterior wall materials considering defects and maintenance. In previous studies, the evaluation of exterior wall materials includes only subjective standard by customers and architects; The economic assessment does not consider the life cycle cost or maintenance cost of the material. As a result, there has been a decline in value, such as building defects and rising maintenance costs. In this study, authors propose a comprehensive economic evaluation that considers material properties, defect occurrence, maintenance cost, and life cycle cost, which are not considered in previous studies, when judging the value of exterior wall materials. The economic evaluation method of the exterior wall materials proposed in this study can be used as a decision model.
Shi Hye Jin,Kim Nam Yee,Eom Sun Ah,Kim-Jeon Myung Deok,Oh Sung Suck,Moon Bag Sou,Kwon Mun Ju,Eom Joong Sik 대한의학회 2022 Journal of Korean medical science Vol.37 No.21
Background: Since the global coronavirus disease 2019 (COVID-19) pandemic, nonpharmacological interventions (NPIs) such as extensive and comprehensive hand hygiene, mask-wearing, and social distancing have been implemented globally. This study aimed to investigate changes in respiratory viruses other than severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) that occurred following the implementation of these NPIs. Methods: From January 2018 to December 2021, influenza-like illness patient specimens and specimens from the Korea Influenza and Respiratory Viruses Surveillance System were analyzed at the Incheon Metropolitan City Institute of Public Health and Environment. Oropharyngeal or nasopharyngeal swab samples from respiratory infection patients were transferred in a virus transport medium at 4°C. After RNA or DNA extraction, respiratory virus-specific genes for human influenza virus (IFV), adenovirus (ADV), parainfluenza virus (PIV), respiratory syncytial virus (RSV), human rhinovirus (hRV), human coronavirus, human bocavirus, and human metapneumovirus were detected by individual real-time reverse transcription polymerase chain reaction. Results: A total 3,334 samples were collected. After NPI was implemented, the detection of respiratory viruses other than SARS-CoV-2 decreased overall. The yearly detection rate of respiratory viruses was decreased from 69.5% (399/574) in 2018 and 73.3% (505/689) in 2019 to 19.8% (206/1,043) in 2020 and 34.9% (365/1,028) in 2021. The epidemic was more prominent in respiratory viruses such as IFV and RSV, which were considered dominant viruses, especially those with viral envelopes. Among viruses that were not considered dominant, hRV showed no clear change before and after NPI, while PIV showed a rapid increase compared to the existing dominant viruses between October–December 2021, after the increase in the number of gatherings started at the end of September and the “Relaxing COVID19 and mitigation policy,” which was implemented on November 1. Conclusion: NPI seems to have influenced the isolation and transmission of respiratory viruses in South Korea. In the future, additional studies focusing on the isolation and transmission patterns of respiratory viruses following NPI are needed.
Kang, Sa-Haeng,Jeon, Yong-Deok,Moon, Kwang-Hyun,Lee, Jeong-Ho,Kim, Dae-Geun,Kim, Wook,Myung, Hyun,Kim, Jong-Sung,Kim, Hyun-Ju,Bang, Keuk-Soo,Jin, Jong-Sik Mary Ann Liebert, Inc. Publishers 2017 Journal of Medicinal Food Vol. No.
<P>Inflammatory bowel disease, including Crohn's disease and ulcerative colitis (UC), is a group of inflammatory conditions of the colon and small intestine. UC is a chronic inflammatory disorder of the colon and rectum that includes intervals of acute exacerbation. Although recent studies have suggested that proinflammatory cytokines might have initiated the inflammatory responses in UC, its etiology remains unclear. Aronia berries are rich in dietary polyphenols such as phenolic acids, anthocyanins, flavonoids, and proanthocyanidins with various health benefits, including antioxidant, anti-inflammatory, and antiaging activities. The objective of this study was to determine whether Aronia berry can be an effective intervention for the treatment of UC. BALB/c mice were administered 5% dextran sulfate sodium (DSS) to induce UC. They were then given Aronia berry extracts at concentrations of 10 or 100 mg/kg. During the induction of UC, the expression levels of nuclear factor-kappa B were increased in colonic epithelial cells and immune cells, leading to increased proinflammatory cytokine levels. Aronia berry extract significantly improved the clinical signs of DSS-induced UC, including body weight loss, colon length shortening, and disease activity index increase, with histological markers of colon injury. Furthermore, oral administration of Aronia berry extract inhibited prostaglandin E-2 production in DSS-induced colitis and decreased the levels of nitric oxide, interleukin-6, and tumor necrosis factor-alpha in lipopolysaccharide-stimulated macrophages. These results suggest that Aronia berry extract could efficiently ameliorate clinical signs and inflammatory mediators of UC. Therefore, Aronia berry might be a promising natural treatment for UC.</P>
박영일,전호종,Seung Jegal,Myung-Deok Kim-Jeon,Jong Yul Roh,Wook-Gyo LEE,Seo Hye PARK,Seong Kyu AHN,Jinyoung LEE,Young Woo GONG,Mun Ju Kwon,Tong-Soo KIM 한국곤충학회 2020 Entomological Research Vol.50 No.1
Owing to global climate change, the global resurgence of vector-borne infectious diseases and their potential to inflict widespread casualties among human populations has emerged as a pivotal burden on public health systems. In this study, the prevalence of flaviviral diseases transmitted by mosquitoes, and their target vector diversity, abundance, and distribution was investigated to enable the mapping of hotspots for these diseases. For the surveillance of the vector mosquitoes carrying flaviviruses during April to November 2015, female mosquitoes were collected to study whether they carried pathogens from abroad at seven locations in Incheon Metropolitan City (Incheon) as a typical urban area and Hwaseong-si (= city, Hwaseong) of Gyeonggi-do (= province) as a rural area. A total of 15 species belonging to seven genera (29,102 female mosquitoes) were collected with blacklight and BG-Sentinel™traps at a collection rate of 260 per trap/night from whole collection locations. The most collected mosquito species in Incheon were Aedes vexans nipponii (species ratio (SR), 29.9%) and the Culex pipiens complex (SR, 28.8%), followed by Anopheles sinensis s.l. (SR, 27.9%) and Ochlerotatus koreicus (SR, 7.1%). From the results of viral RNA detection, five flaviviruses were found in 20,981 individuals (excluding An. sinensis 696 pools) in the Cx. pipiens complex and Ae. vexans nipponii. Three Japanese encephalitis virus (JEV)-positive pools were from the Cx. pipiens complex, a Chaoyang virus pool was found from Ae. vexans nipponii, and the remaining unidentified flavivirus pool was from Cx. pipiens. The three JEV-positive pools were phylogenetically grouped as genotype V. The results of our study demonstrate that enhanced monitoring and long-term surveillance of these vector viruses are of great public health importance.
전호종,Seung Jegal,Myung-Deok Kim-Jeon,Jong Yul Roh,Wook-Gyo LEE,Seo Hye Park,Seong Kyu Ahn,Jinyoung LEE,Young Woo GONG,Mun Ju Kwon,박영일,Tong-Soo Kim 한국곤충학회 2020 Entomological Research Vol.50 No.2
Owing to climate change, the global resurgence of vector-borne infectious diseases has emerged as a critical public health issue. Orientia tsutsugamushi is the etiological agent of tsutsugamushi disease (scrub typhus) a mite-borne acute febrile disease occurring in the Asia-Pacific region. We investigated the prevalence of tsutsugamushi disease transmitted by chigger mite vectors living on rodents. Using sticky-type chigger traps for three months during 2016–2018, 1,057 chigger mites were collected (chigger mite index, 1.31) from four locations in the Hwaseong-si area of Gyeonggi-do, Republic of Korea. Five species distributed among three genera were identified. In addition, 94 rodents were captured (collection rate: 7.83%) using Sherman live traps over the course of three months (April, October, and November) during 2016–2017. Three rodent species were captured and identified and the striped field mouse (Apodemus agrarius) was the dominant rodent host species in the surveyed area. A total of 10,469 ectoparasitic chigger mites were recovered from the 94 rodents, from which 13 species distributed among four genera were identified. Of the 5,250 chigger mites examined, Leptotrombidium pallidum was most abundant (n = 2,558), followed by L. orientale, L. scutellare, L. zetum, Euschoengastia koreaensis, L. subintermedium, and Neotrombicula tamiyai. Of the examined chigger mites, no groups recovered from rodent hosts tested positive for O. tsutsugamushi. This study provides fundamental regional information on vector-borne disease data collection in the Hwaseong-si area, Gyeonggi-do, and will further contribute to formulating disease control and prevention strategies.