백서 혈관평활근 세포에서 α-Lipoic acid가 PAI-1 발현, 세포의 증식, 주유능 및 신생내막 형성억제에 미치는 효과

신동우,이동욱,이상준,김혜순,강효경,안종덕,이인규 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:당뇨병의 혈관 합병증의 발생에 있어서 산화스트레스는 중요한 역할을 하는 것으로 알려져 있다. 혈관 평활근세포에서 고혈당은 안지오텐신Ⅱ와 더불어 활성산소족(ROS)을 증가시키며, 산화스트레스에 민감한 전사인자들을 활성화시켜 동맥경화증을 유발하는 것으로 알려져 있다. 이에 저자들은 항산화제로 알려진 알파­리포산이 혈관 평활근세포의 증식속도와 이주에 미치는 영향과 PAI­1발현에 미치는 영향을 조사하였고 이와 동시에 백서의 혈관 손상 모델을 이용하여 알파­리포산이 신생내막 증식을 억제시킬 수 있는지를 알아보았다. 방법:In vitro 실험은 백서의 대동맥 평활근세포를 고농도의 포도당(22mM)과 100nM의 안지오텐신Ⅱ로 4시간 배양하였다. 알파­리포산을 처리 후, PAI­1 mRNA의 발현을 보기 위해 노던 블롯을 시행하였고 평활근세포의 유주능과 증식속도에 미치는 영향을 보기 위해 성장속도의 유주능 분석을 시행하였다. 또한 평활근세포의 유주능에 NF­μB 경로가 미치는 영향을 보기 위해 겔 지연 분석과 NF­μB 보고 유전자 분석을 시행하였다. In vivo 실험으로 백서의 혈관손상 모델을 이용하여 알파­리포산 처리 후 혈관의 신생내막 두께를 비교하였다. 결과:In vitro 실험에서 고농도 포도당과 안지오텐신Ⅱ에 의해 유도된 PAI­1 mRNA 발현증가는 알파­리포산 처리에 의하여 용량에 비례하여 억제되었다(p<0.05). 알파­리포산 처리로 혈관 평활근세포의 유주능은 유의하게 억제되었으나(p<0.01),증식속도는 유의하게 억제되지 않았다. 또한 알파­리포산 처리로 NF­μB 발현도 유의하게 억제되었다(p<0.01). In vivo 실험에서 알파­리포산을 주입한 군에서 혈관손상에 의한 신생내막의 증식이 유의하게 억제되는 것을 보여주었다(p<0.01). 결론:알파­리포산은 백서의 대동맥 평활근 세포의 증식속도는 억제하지 못했으나 유주능은 유의하게 억제하였으며, 혈관 손상 모델에서 신생 내막 증식도 유의하게 억제함을 확인할 수 있었다. 이러한 작용은 알파­리포산에 의한 NF­μB 경로의 억제와 연관이 있는 것으로 사료된다. Background : Exposure to large amounts of glucose causes a characteristic dysfunction and morphologic changes of the endothelium by an increased production of reactive oxygen species(ROS) in diabetes. The plasminogen activator inhibitor-1(PAI-1), which modulates fibrinolysis and cell migration may influence proteolysis and neointimal formation in vascular smooth muscle cells(VSMC). Antioxidants have been proposed to inhibit multiple proatherogenic events. This study investigated the effect of α-lipoic acid on PAI-1 expression and VSMC proliferation and migration both in vivo and in vitro. Methods : In the in vitro study, cultured rat aortic smooth muscle cells(RASMC) were incubated in a medium containing high glucose (22 mM) and 100 nM angiotensin Ⅱ for 4 hour. After α-lipoic acidtreatment, a -migration and growth assay of the RASMC, and a gelmobility shift assay and reportergene analysis for nuclear factor- иB(NF- иB) and northern blot analysis for PAI-1 were performed. In the in vivo study, the effect of α-lipoic acid on neointimal hyperplasia in a rat carotid balloon injury model was evaluated. Results : RASMC migration was inhibited significantly by α-lipoic acid (p<0.01), but their prolife ration was not inhibited. The NF-иB DNA binding activity and NF-иB promoter activity was inhibited by α-lipoic acid significantly (p<0.01). α-lipoic acid inhibited PAI-1 mRNA expression by high glucose and angiotensin Ⅱ in dose dependent manner (p<0.05). In the rat carotid artery balloon injury model, neointimal formation was reduced by α-lipoic acid treatment in a dose dependent manner significantly (p<0.01). Conclusion : α-lipoic acid suppresses migration, but not prolife ration in RASMC. α-lipoic acid also reduce neointima formation in a rat carotid balloon injured model. This effect might be related to the blocking of NF-иB which increase the expression of the genes associated with atherosclerosis including TNF-α, IL-1, IL-6, endothelin-1, MCP-1, VCAM-1, ICAM-1, E-selectin, tissue factor(J Kor Diabetes Asso 25:446~459, 2001).

중학생이 지각한 부모의 학대와 자녀의 부적응

강경아,류점숙,신효식 한국 가정과 교육 학회 1998 한국가정과교육학회지 Vol.10 No.2

This study investigated the relationship between parental abuse and maladjustment perceived by school student. The subjects were 448 middle school students in Kwangju. The main results of this were as follows ; 1. There were found significant differences according to background variables in parental abuse and maladjustment. Physicalabuse was found significantly different according to gender of the child and unreasonable rearing behavior of the parents, but emotional abuse was found significantly different according to job of the father and unreasonable rearing behavior of the parents. And, neglect was found significantly different according to educational level of the father and unreasonable rearing behavior of the parents. Social withdrawal was found significantly different according to gender of the child, family income and unreasonable rearing behavior of the parents, but hyperactivity was found significantly different according to gender of the child and unreasonable rearing behavior of the parents. Agression and reteat were found significantly different according to gender of the child and unreasonable rearing behavior of the parents. Obsesive-compulsion was found significantly different according to unreasonable rearing behavior of the parents. 2. There were significantly positive correlation between parental abuse and maladjustment. 3. The finding of multiple regression analysis demonstrated that neglect, physical abuse, family income, gender of child were found to be important variables in predicting social with drawal. Neglect, unreasonable rearing behavior of the parents, physical abuse, gender of child were found to be important variables in predicting hyperactivity. Neglect, emotional abuse, and unreasonable rearing behavior of the parents were found to be important variables in predicting aggression. Gender of the child, emotional abuse, and unreasonable rearing behavior of the parents were found to be important variables in predicting retreat. And, unreasonable rearing behavior of the parents, neglect, and physical abuse were found to be important variables in predicting obsessive-compulsion.

미술활동을 통한 ADHD 특성 아동의 문제행동 개선 연구

강은주,이효신 한국특수아동학회 2002 특수아동교육연구 Vol.4 No.2

본 연구는 ADHD 아동의 문제행동을 개선하기 위해 미술활동을 적용한 것이다. ADHD 아동들이 문제행동을 나타내는 것은 주의력결함이나 충동성, 즉 행동억제와 관련된 실행기능상의 문제로 파악되므로 미술활동을 통하여 자신의 행동에 대한 통제 훈련이 가능할 것이라는 점이 전제되었다. 대상아동은 DSM-Ⅳ의 진단기준과 주의력결핍과잉행동교사평정지의 선별기준을 만족시키는 3명의 아동으로 선정하였고, 미술활동 프로그램은 활동과정상 소근육운동의 활성과 억제가 요구되는 활동으로 구성하였다. 실험은 3명의 대상아동에 대하여 AB설계법으로 이루어졌으며 중재기간 후, 변화된 행동이 유지되는지도 알아보았다. 문제행동은 자리이탈과 주의산만행동으로 제한하고, 측정은 프로그램 중재 후 수업시간 20분을 비디오 촬영하여 연속기록법으로 행동발생의 빈도를 기록하였다. 연구결과, 3명의 대상아동 모두가 중재기간동안에 문제행동이 평균적으로 감소하였고, 중재를 중단한 유지기간동안에도 문제행동이 기초선수준으로 증가하지 않는 양상을 나타내었다. 따라서 미술활동은 ADHD 아동의 문제행동, 특히 자리이탈과 주의산만 행동을 감소시키는 데에 효과가 있다. The purpose of this study is to investigate the effects of various art activities on the leaving from seats and the distraction of an ADHD child. The subjects of the study was three ADHD children in W elementary school, showed problematical in 9 items regarding attention deficit of DSM-Ⅳ, over 6 items each regarding hyperactivity disorder and impulse and 9 items out of 14 items in an evaluation paper for attention deficit and hyperactivity disorder. The program was composed of art activities effective on improvement of attention power and decrease of hyperactivity disorder. The art activity program was divided into 4 steps; the beginning step(1-4 period), the expression step(5-12 period), the variation step(13-20 period), the completion step(21-23 period) of all 23 periods for 40 minutes a time, 4 times a week from 2002, 5, 27. to 2002, 7, 10. and it was applied by myself. The execution of the study was carried into foundation, arbitration, maintenance stages by AB design mode of single subject study method. Occurrence frequency of the disordered acts of the subjects was measured by continuative recording method. 20 minutes' school class in the foundation and maintenance stages, 40 minutes' program activities and 20 minutes' school class in the arbitration stage were recorded on a video and frequency degree of the act of separating from seats and the act of distraction by each period was written down by an observer. As a result of execution of the study, it showed that the program of various act activities was effective on decrease in the leaving from seats and the distraction of an ADHD children.

전문비서직에 대한 이대 비서학과 학부학생들의 의식구

구효선,김경화,윤혜정,이지연,정은희,강은정,김윤희,신해정 이화여자대학교 법정대학 비서학과 1997 비서학연구 Vol.- No.17

枸杞 Lycium chinense Miller의 果實이 實驗的 糖尿에 미치는 影響

李珩九,姜孝信,鄭址昌 中央醫學社 1975 中央醫學 Vol.28 No.6

The influence of fruit of Lycium chinense Miller on the blood sugar levels and serum transaminase (SGP-T, SGO-T) activities from alloxan-diabetic rabbits were observed. The Lycii Fructus were extracted with methanol, and the extracted sample was orally administrated to normal and alloxan diabetic rabbits. As for the blood sugar levels and serum transaminase activities of normal rabbits no significant variation were detected by administration of the extracted sample. The blood sugar levels and serum transaminase activity of normal rabbits were significantly increased by administration of the alloxan. The increased blood sugar levels of 'the alloxan-diabetic rabbits were gradually decreased by administration of the extracted samples, and were returned to the control levels after 12 day. The increased transaminase activities in serum of the alloxan-diabetic rabbits were significantly decreased after 96 hours. According to the above results, it would be concluded that the extract of Lycii Fructus influences for the recovery of the increased amount of blood sugar levels and serum transaminase activities by alloxan.

시간지연 제어기법을 이용한 유연한 로봇 팔의 위치 제어

김동기,강이석,신효필 忠南大學校 産業技術硏究所 1995 산업기술연구논문집 Vol.10 No.2

A TDC(Time Delay Control) theory is applied to the the position control problems of flexible robot manipulators subjected to unknown dynamics and distrurbances. One link flexible manipulator system with tip mass is represented as a simple spring-mass-damper syseem and AC servo motor is used as the actuator. Based on the system model, the TDC is designed, and the PI-controller is also designed for comparison. The control performance of TDC is evaluated through a series of simulations and experiments. The simulational and wxperimental results show that the TDC is much robust than the PI-controller to the unknown dynamics and disturbances.

柴胡가 Alloxan投與家兎 血淸中 Cholesterol 含量 및 Transaminase 活性度에 미치는 影響

李京燮,姜孝信,鄭址昌 中央醫學社 1975 中央醫學 Vol.29 No.1

The effects of root of the Bupleurum falcatum L. on the serum transaminase (SGP-T, SGO-T) activities and the content of total cholesterol in serum from normal and alloxan-diabetic rabbits were observed. The Bupleuri Radix were extracted with methanol, and the extracted samples were orally administrated to the normal and alloxan-diabetic rabbits. The blood sugar levels, the amount of total cholesterol in serum and the serum transaminase activities of normal rabbits were significantly increased by intravenous administration of the alloxan 80mg/kg. The increased serum transaminase activities of the alloxan-diabetic rabbits were significantly decreased after 96 hours by administration of the extracted sample compared to that of control group. The increased content of total cholesterol in serum of the alloxan-diabetic rabbits were gradually decreased by administration of the extracted samples and returned to the control levels ,after 12 days. According to the above results, it is concluded that the extract of Bupleuri Radix have influence to the recovery of the increased contents of total cholesterol and serum transaminase activities by alloxan.

증발과 응결에 대한 국민 학생들의 개념 조사

최병순,김효남,강순희,신인철 한국교원대학교 과학교육연구소 1993 청람과학교육연구논총 Vol.3 No.1

The purpose of this study was to investigate and analyze the conception of elementary school students on evaporation and condensation, and then to verify the types of the conception. Forty-eight children from six elementary schools were sampled by stratified random sampling in Seoul and other provinces. They responded to the questionaire and the interview. This study was carried out according to the process of the basic study, the preliminary study, and the main study. The materials collected were classified and analyzed according to the types of children's ideas. The findings of this study were as follows: 1. The vocabulary used to describe the evaporation phenomena varied according to the situations, and the scientific term "evaporation" was more frequently used by the older groups. 2. Most children answered that the last location of water were air/sky/cloud. Air/cloud which represents the scientific conception of the location of water were mentioned by children of all ages. The higher the grade of the children were, the more scientific conception the children mentioned, however. 3. Most children referred to the heat as the factor of evaporation. Wind, on the other hand was mentioned by less than 10% of lower graders, and by about 30% of higher graders. 4. The result of asking children whether they thought it was possible to get the evaporated water back showed that about 70% of lower graders denied the possibility of the water being reversible. About 60% of higher graders, however, recognized that the water will be returned as rain or condensation. This increase may be associated with formal teaching of water cycle. 5. In the ideas of evaporation and condensation, some of the children have supernatural ideas and animism, which are most younger chilren's characteristics.

뇌졸중 환자의 인지 기능과 시공간 지각의 관계

노효련,나눈별,김정윤,김은진,조원재,강신욱,Roh, Hyo-Lyun,Na, Nun-Byeol,Kim, Jung-Yoon,Kim, Eun-Jin,Jo, Won-Jae,Kang, Shin-Wook 한국임상보건과학회 2013 한국임상보건과학회지 Vol.1 No.1

Purpose : The aim of this study was to identify the relation of cognition and visuospatial perception in stoke patients. Methods : A total of 16 stroke patients recruited from two hospital located in Donghae, Kang-won participated in this study(Right hemiplegia : twelve, Left hemiplegia : four). All participants scored higher than 20 point on the MMSE-K. To assess the cognitive function, we performed the Korean version of Mini Mental State Examination(MMSE-K), spatial perception domain of Lowenstein Occupational Therapy Cognition Assessment(LOTCA), Figure Color Copy(FCC), Linebisection Test(LBT) and Albert's test in stoke patients. Results: The Korean version of Mini Mental State Examination and spatial perception domain of Lowenstein Occupational Therapy Cognition Assessment were the highest points in sixty ages, Figure Color Copy is difference between Right hemiplegia and Left hemiplegia. Linebisection Test(LBT) and Albert's test had much relation, visuospatial perception had not relationship. Conclusion: Aging was cognition function and visuospatial perception had an interrelationship. visuospatial perception according to affected side in brain had an interrelationship but cognition function and visuospatial perception had not any interrelationship.

Three-dimensional and topographic relationships between the orbital margins with reference to assessment of eyeball protrusion

Kang Jae Shin,Shin Hyo Lee,Ki Seok Koh,Wu Chul Song 대한해부학회 2017 Anatomy & Cell Biology Vol.50 No.1

This study investigated the topographic relationships among the eyeball and four orbital margins with the aim of identifying the correlation between orbital geometry and eyeball protrusion in Koreans. Three-dimensional (3D) volume rendering of the face was performed using serial computed-tomography images of 141 Koreans, and several landmarks on the bony orbit and the cornea were directly marked on the 3D volumes. The anterior-posterior distances from the apex of the cornea to each orbital margin and between the orbital margins were measured in both eyes. The distances from the apex of the cornea to the superior, medial, inferior, and lateral orbital margins were 5.8, 5.8, 12.0, and 17.9 mm, respectively. Differences between sides were observed in all of the orbital margins, and the distances from the apex of the cornea to the superior and inferior orbital margins were significantly greater in females than in males. The anterior-posterior distance between the superior and inferior orbital margins did not differ significantly between males (6.3 mm) and females (6.2 mm). The data obtained in this study will be useful when developing practical guidelines applicable to forensic facial reconstruction and orbitofacial surgeries.