새마을기계화영농단의 적정모델설정에 관한 연구

김철기, 차균도, 유진채, 박명근 忠北大學校 새마을硏究所 1985 새마을 硏究 論文集 Vol.4 No.-

본문참고

청주공업단지조성이 주변농촌의 농업구조에 미친 영향 -농촌공업입지와 농가취업권조사를 중심으로-

김철기, 유진채, 박명근 忠北大學校 새마을硏究所 1985 새마을 硏究 論文集 Vol.4 No.-

본문참고

유방암종에서 p53, cyclin D1 및 cathersin D 단백발현의 의의

문경래,김환정,이미자,기근홍,서재홍,양정원,조현진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives: Breast carcinoma is a heterogenous disease. The prognosis of this carcinoma correlates with various prognostic factors. The aim of this study was to assess the prognostic significance of expression of p53, cyclin Dl and cathepsin D protiens in association with various prognostic factors in invasive breast carcinoma. Materials and Methods: The relationship is presented between expression of p53, cyclin D1 and cathepsin D by immunohistochemistry and various established prognostic factors such as tumor size, histologic grade, lymph node status and extensive intraductal component in 55 cases of invasive breast carcinoma. Results: No significant correlations were observed between expression of p53, cyclin Dl and cathepsin D and tumor size. The expression of p53 correlated with high histologic grade. The expression of p53 and cyclin Dl correlated with lymph node metastasis. Conclusion: These results indicate that expression of p53 and cyclin Dl in invasive ductal carcinoma may be an important prognostic factor, closely related to the high histologic grade and lymph node metastasis.

Cubic Convolution Scaler Optimized for Local Property of Image Data

Jin-Kee Chae,Jae-Yung Lee,Min-Ho Lee,Jong-Ki Han,Nguyen, Truong Q.,Woon-Young Yeo IEEE 2015 IEEE TRANSACTIONS ON IMAGE PROCESSING - Vol.24 No.12

<P>A scaler is one of the most important modules in various video applications, such as ultra-high definition TV and scalable video systems. A variety of scaling techniques have been used to increase the video quality when the resolution of the source image has to be up- and down-scaled. Some conventional schemes exploit the property of local block data. Others consider the edge information of the data to be scaled. In this paper, we formulate a scaling problem to minimize the information loss resulting from the resizing process. The loss is considered in both the spatial and the frequency domains, and then it is minimized to optimize the kernel of the scaler. The simulation results show that the proposed algorithm reduces the information loss more than conventional schemes. When compared with the conventional algorithms, the proposed method outperforms those with similar complexity.</P>

삼해주와 시판 곡주의 생리 기능성 및 세포 독성 효과

임채란(Chae-Lan Lim),손희진(Hee-Jin Son),조인영(In-Young Cho),김계원(Gye-Won Kim),최수진(Soo-Jin Choi),김인선(In-Sun Kim),한기영(Kee Young Han),최진영(Jin Young Choi),노봉수(Bong Soo Noh) 한국식품과학회 2009 한국식품과학회지 Vol.41 No.6

잊혀져가고 있는 전통주의 우수함을 알리고자 한국의 전통 반가주인 삼해주를 제조하고 시판되고 있는 곡주와 비교 실험하며, 생리활성 및 세포독성에 관한 연구를 진행하였다. 기존의 문헌에 소개된 방법을 변형하여 3가지 종류의 삼해주를 제조하였으며, 시중에서 곡주 5종을 구입하여 실험하였다. DPPH 라디칼 제거능과 아질산염 소거능은 곡주 G가 가장 뛰어났으며, ABTS 라디칼 제거능은 삼해주가 가장 우수하였다. 삼해주 중에서는 C 시료가 DPPH 라디칼 제거능, 아질산염 소거능, ABTS 라디칼 소거능이 가장 뛰어났다. 혈전용해능의 경우 삼해주 A-C가 13-17U로 다른 곡주에 비해 그 활성이 높게 나타났다. 각 시료에 대하여 10-160배까지 단계적으로 희석하여 HeLa, A549, L-132 세포에 처리하였을 때, 인체유래 자궁암 세포주인 HeLa 세포의 경우 10배 희석액에서 삼해주가 강한 세포 독성을 보였고, A549 세포의 경우 10배 희석액에서 삼해주의 세포독성 효과가 크나 시료에 따라 그 편차가 심하였다. L-132의 경우 10배 희석액에서 삼해주의 세포독성 효과가 다른 시료에 비해 강하였으나, 다른 암세포에 비해 약한 것을 알 수 있다. 본 연구의 결과에 의하면 삼해주에 존재하는 미지의 약리 성분이 생리 활성 및 암세포의 성장에 영향을 미치는 것으로 판단되었다. 이는 삼해주 제조 시 사용된 누룩과 오랜 발효 기간에 의한 영향으로 생각되며, 누룩에 대한 연구가 더 진행되어야 할 것으로 여겨진다. This study was conducted to investigate the antioxidant activity, fibrinolytic activity and cytotoxic effect of Korean traditional noble rice wine made using different methods (A-C) and commercial rice wine (D-H) on various tumor cell lines. The antioxidant activity of rice wine was measured by DPPH (2,2-dipicryl-1-picrylhydrazyl), ABTS [2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid)] and NO (nitric oxide) radical scavenging assay. In this study, Samhaeju showed the greatest fibrinolytic activity of 13-17U and exhibited the highest antioxidant activity. Among the different Samhaeju, the sample prepared using method C had the highest antioxidant activity. The cytotoxic effect of rice wine were also examined against the human cancer cell line (A549 cells and HeLa cells) based on the results of a WST-1 assay and morphological changes. Rice wine induced the inhibition of cell proliferation and morphological changes in tumor cell lines in a concentration-dependent manner, with Samhaeju diluted 10 fold having the strongest effect on these factors. These findings suggest that Korean rice wine has antioxidant activity and cytotoxic effect, and that these factors are influenced by the method of preparation.

상박골 외과부 골절의 수술적 치료에 대한 고찰

채한기,이광진 충남대학교 의과대학 지역사회의학연구소 1976 충남의대잡지 Vol.3 No.2

Fractures of lateral condyle of humerus occur almostly in childhood and complications such as non-union, malunion, occur commonly in displaced fractures of lateral humeral condyle which were not reducted anatomically. But, such complications and followed problems were preventable by early accurate open or closed anatomicalr reduction. Also open reduction and internal fixation with screw, K-wire cat-gut suture or pin have been recommened as best method by many surgeons on displaced fractures of lateral condyle of humerus. We were carried out surgical treatment of open reduction and interal fixation on 25 cases of displaced lateral condyle fractures of humerus and followed 18 cases from Jan, 1968 to Dec., 1975 at department of orthopedic surgery, Chung-Nam National University Hospital and results were observed as follows. 1. of the 18 cases, the frequency was high in male as 13 males and 5 females, and were founded in the age of 3 to 15 years. 2. of the 18 cases, the causes of trauma were sliding down in 12 cases and fall from height in 4 cases. 3. Milch's type Ⅰ fracture were founded in 4 cases and type Ⅱ fractures in 14 cases. Also right side was affected more than left side. 4. Duration between trauma and treatment were 4 weeks in 13 cases and 3 months over in 2 cases. 5. 9 cases were unioned normally in accurately reducted 11 cases and of poorly reducted 7 cases, 6 cases were revealed poor results. 6. Average duration of cast immobilization were 6 to 9 weeks. 7. Average follow-up length were 34 months. 8. of normally unioned 11 cases, 9 cases received treatment before 4 weeks after trauma. 9. of 18 cases, 11 cases were gained full range of motion, and 2 cases were revealed over 10 degrees limitation of flexion and extension. 10. Surgical complication were nonunions in 5 cases, malunions in 2 cases, lump oven lateral condyle in 5 cases, tardy ulnar nerve palsy in 3 cases and wound infection in 1 case.

High dietary inorganic phosphate increases lung tumorigenesis and alters Akt signaling.

Jin, Hua,Xu, Cheng-Xiong,Lim, Hwang-Tae,Park, Sung-Jin,Shin, Ji-Young,Chung, Youn-Sun,Park, Se-Chang,Chang, Seung-Hee,Youn, Hee-Jeong,Lee, Kee-Ho,Lee, Yeon-Sook,Ha, Yoon-Cheol,Chae, Chan-Hee,Beck, Geo American Lung Association 2009 American journal of respiratory and critical care Vol.179 No.1

<P>RATIONALE: Phosphate (Pi) is an essential nutrient to living organisms. Recent surveys indicate that the intake of Pi has increased steadily. Our previous studies have indicated that elevated Pi activates the Akt signaling pathway. An increased knowledge of the response of lung cancer tissue to high dietary Pi may provide an important link between diet and lung tumorigenesis. OBJECTIVES: The current study was performed to elucidate the potential effects of high dietary Pi on lung cancer development. METHODS: Experiments were performed on 5-week-old male K-ras(LA1) lung cancer model mice and 6-week-old male urethane-induced lung cancer model mice. Mice were fed a diet containing 0.5% Pi (normal Pi) and 1.0% Pi (high Pi) for 4 weeks. At the end of the experiment, all mice were killed. Lung cancer development was evaluated by diverse methods. MEASUREMENT AND MAIN RESULTS: A diet high in Pi increased lung tumor progression and growth compared with normal diet. High dietary Pi increased the sodium-dependent inorganic phosphate transporter-2b protein levels in the lungs. High dietary consumption of Pi stimulated pulmonary Akt activity while suppressing the protein levels of tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 as well as Akt binding partner carboxyl-terminal modulator protein, resulting in facilitated cap-dependent protein translation. In addition, high dietary Pi significantly stimulated cell proliferation in the lungs of K-ras(LA1) mice. CONCLUSIONS: Our results showed that high dietary Pi promoted tumorigenesis and altered Akt signaling, thus suggesting that careful regulation of dietary Pi may be critical for lung cancer prevention as well as treatment.</P>

해양의 Pseudomonas sp. 로부터 분리한 alginate lyase 유전자의 promoter에 의한 대장균 내에서의 β-agarase 유전자의 발현과 catabolite repression의 변화

진철호,박제현,한정현,최윤혁,이종희,이정기,공인수 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.2

Strong promoter로 밝혀진 alginate lyase유전자의 promoter부위에 대한 특성을 검토하기 위해 alginate lyase유전자의 46개 N-terminal amino acid가 포함된 promoter부분과, 같은 균으로부터 분리한 β-agarase의 유전자를 연결시켜 agarase의 activity를 평판배지상에서 보다 쉽게 확인하는 방법으로 promoter의 활성을 측정한 결과 alginate lyase유전자 promoter에 의해서 β-agarase유전자의 대량발현이 유도되고 있었으며 glucose의 존재하에서 β-agarase유전자 발현이 일어나지 않는 catabolite repression양상을 나타내고 있다. PCR로써 alginate lyase 유전자의 promoter하류에 존재하는 alginate lyase의 46개 N-terminal amino acid 부분이 순차적으로 제거된 plasmid를 제조하여 대량발현을 조사한 결과 46개의 아미노산이 제거된 후에도 β-agarase의 활성에는 변화가 없어 46개의 N-말단이 정상적인 상태에서 발현에는 영향을 미치고 있지 않음을 확인할 수 있었다. 또한 alginate lyase유전자의 promoter region에 존재하는 가능한 2개의 promoter consensus sequence PⅠ, PⅡ를 subcloning한 결과 promoter PⅡ만이 존재할 때도 대량발현이 유도되고 있음을 확인할 수 있었으며 동시에 glucose가 존재할때 catabolite repression이 역시 나타나고 있어 이 부분이 발현 및 glucose에 의한 regulation에 매우 중요하게 작용하는 부분이라는 것을 확인할 수 있었다. Promoter is a key factor for expression of the recombinant protein. There many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter and association between the promoter mutants, β-agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids of the alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using PCR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of β-agarase productivity.

Eccrine hidrocystomas of Robinson type improved with intense pulsed Light (초)

( Chae Hwa Kim ),( Woo Jin Yun ),( Chong Hyun Won ),( Sung Eun Chang ),( Ho Seok Suh ),( Mi Woo Lee ),( Jee Ho Choi ),( Kee Chan Moon ) 대한피부과학회 2009 초록집 Vol.47 No.10

알코올 대사 효소들의 유전적 다형성이 음주 행태 및 간경변증 발생에 미치는 영향

기주영,김민옥,유일영,채지영,홍의실,안성철,김헌,박선미,윤세진,채희복 대한간학회 2003 Clinical and Molecular Hepatology(대한간학회지) Vol.9 No.2

목적: 만성적인 다량의 음주에도 불구하고 알코올 중독자의 15%만이 알코올성 간경변증에 이르는데, 유전학적인 차이를 포함한 간질환에 대한 개인적인 감수성의 차이를 포함한 간질환에 대한 개인적인 감수성의 차이가 발병에 중요한 영향으리 미칠 것으로 생각된다. 이에 저자들은 정상 대조군, 과음주력이 있으나 간경변증의 증가가 없는 군, 알코올성 간경변증 환자군 간의 ALDH2, ADH2, CYP2E1, catalase의 네 가지 알코올 대사 효소의 유전자형 빈도 차이가 음주 행태 및 질환 발생을 어느 정도 설명할 수 있는 것인지를 알아보고자 하였다. 대상과 방법: 정상 성인 42명을 정상 대조군(평균연령 52±9세)으로, 임상적 혹은 방사선학적으로 알코올성 간경변증이 확실하게 증명된 30명을 알코올성 간경변증군(평균연령 52±9세)으로, 음주력은 있으나 간질환의 증거가 없는 12명을 과음주자군(평균연령 46±6세)으로 배정하였다. 환자의 말초혈액 백혈구에서 DNA를 추출한 후, ALDH2, CYP2E1, ADH2, catalasel 유전자에 대해 PCR을 시행한 후 제한 효소들로 처리하여 제한 분절길이 다형성으로 유전자형을 나누어 각 유전자형의 출현 빈도를 비교하였다. 결과: 1) 본 연구에서 검사한 정상 성인 남자 42예에서 알코올 대사 효소들의 유전자형 빈도는 ADH2는 (1*1), (1*2)가 38%, 36%, ADLH2 (1*1)(야생형), (1*2), (2*2)이 각각 69%, 28%, 2%의 비율로 나타났다. CYP2E1의 경우 c1/c1(야생형) 유전자형이 55%를 차지하였다. catalase1의 경우 TT(야생형)유전자형이 12%를 차지하였다. 2) 정상 대조군과 음주자군(과음주자군과 알코올성 간경변증 환자군의 합)간에 ALDH2 (1*2) 혹은 (2*2) 유전자형 빈도가 유의하게 음주자군에서 낮음을 알 수 있었다. 음주자군 중에서는 ALDH2 효소의 결핍형 동형접합체 ALDH (2*2)는 한 예도 없었으며 이형접합체만이 5예였으며, 나머지는 모두 야생형 동형접합체(88%)였다. 3) 정상대조군과 음주자 간에 ADH2, CYP2E1, catalase1 유전자의 유전자형 분포의 차이를 관찰할 수 없었다. 결론: ALDH2 결핍 유전자형 존재는 개인의 음주 행태에 영향을 미치는 인자이나, 존재는 개인의 음주 행태에 영향을 미치는 인자이나, 음주자군에서 그 결핍 빈도가 낮아 알코올성 간경변증 발병에 미치는 영향을 임상 연구를 통하여 규명하기에는 제한점이 있었다. ADH2, CYP2E1, CAT1 유전자의 경우에는 세 군간에 서로 의미 있는 차이가 없어 질병 발생과, 음주 행태에 있어서 이 유전자들의 역할은 미미할 것으로 생각된다. Background/Aims: Genetic variations of ethanol-metabolizing enzymes can affect alcohol drinking behavior. The aims of this study were to investigate and compare the distributions of these genetic polymorphisms between a healthy control group and a heavy drinker group which included an alcoholic liver cirrhosis group. Methods: Genotypes of ADH2, ALDH2, CYP2E1, and catalase were identified by polymerase chain reaction and restriction fragment length polymorphism. Genomic DNA was extracted from peripheral leukocytes in 42 healthy controls, 12 heavy drinkers, and 30 alcoholic liver cirrhosis patients. Results: 1) The genotype frequencies of ALDH2 (1*1), ADH2 (1*1), CYP2E1 (c1c1), and catalase1 (TT) were 69%, 55%, 38%, and 12%, respectively in healthy Korean males. 2) There was a significant difference in the distribution of the genetic polymorphism of ALDH2 between the control group and heavy drinker group (12 heavy drinkers and 30 alcoholic liver cirrhosis patients). The genotype frequency of ALDH2 mutant, ALDH2 (1*2) and ALDH2 (2*2) in the heavy drinker group (12%) was significantly lower than that in the control group (30%). 3) We didn’t find anyone with ALDH2 homozygote mutant (DD) in the heavy drinker group. 4) There was no significant difference in the distribution of genetic polymorphisms in ADH2, CYP2E1 and catalase1 between the two groups. Conclusions: These results suggest that the absence of ALDH2 mutant genotype is strongly related to heavy drinking behavior. We can not prove, however, and evidence that the polymorphisms of other ethanol-metabolizing enzymes are associated with the determination of alcohol-drinking behavior.