http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
진철호,박제현,한정현,최윤혁,이종희,이정기,공인수 한국산업미생물학회 2001 한국미생물·생명공학회지 Vol.29 No.2
Strong promoter로 밝혀진 alginate lyase유전자의 promoter부위에 대한 특성을 검토하기 위해 alginate lyase유전자의 46개 N-terminal amino acid가 포함된 promoter부분과, 같은 균으로부터 분리한 β-agarase의 유전자를 연결시켜 agarase의 activity를 평판배지상에서 보다 쉽게 확인하는 방법으로 promoter의 활성을 측정한 결과 alginate lyase유전자 promoter에 의해서 β-agarase유전자의 대량발현이 유도되고 있었으며 glucose의 존재하에서 β-agarase유전자 발현이 일어나지 않는 catabolite repression양상을 나타내고 있다. PCR로써 alginate lyase 유전자의 promoter하류에 존재하는 alginate lyase의 46개 N-terminal amino acid 부분이 순차적으로 제거된 plasmid를 제조하여 대량발현을 조사한 결과 46개의 아미노산이 제거된 후에도 β-agarase의 활성에는 변화가 없어 46개의 N-말단이 정상적인 상태에서 발현에는 영향을 미치고 있지 않음을 확인할 수 있었다. 또한 alginate lyase유전자의 promoter region에 존재하는 가능한 2개의 promoter consensus sequence PⅠ, PⅡ를 subcloning한 결과 promoter PⅡ만이 존재할 때도 대량발현이 유도되고 있음을 확인할 수 있었으며 동시에 glucose가 존재할때 catabolite repression이 역시 나타나고 있어 이 부분이 발현 및 glucose에 의한 regulation에 매우 중요하게 작용하는 부분이라는 것을 확인할 수 있었다. Promoter is a key factor for expression of the recombinant protein. There many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter and association between the promoter mutants, β-agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids of the alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using PCR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of β-agarase productivity.
진철호,윤영원,이재학 사단법인 유공압건설기계학회 2018 드라이브·컨트롤 Vol.15 No.1
This research describes the development model and testing of a hybrid damper which can be applicable to a vehicle suspension. The hybrid damper is devised to improve the performance of a conventional passive oil damper using a magneto-rheological (MR) accumulator which consists of a gas accumulator and a MR device. The level of damping is continuously variable by the means of control in the applied current in a MR device fitted to a floating piston which separates the gas and the oil chamber. A simple MR device is used to resist the movement of floating piston. At first a mathematical model which describes all flows within the conventional oil damper is formulated, and then a small MR device is also devised and adopted to a mathematical model to characterize the performance of the device.
Indomethacin이 가토의 신장기능에 미치는 영향
진철호,장석종 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2
A number of anti-inflammatory agents such as indomethacin can inhibit prostaglandin synthesis in renal tissue. This study was undertaken to evaluate the effects of indomethcin on the renal function in rabbit. Blood and urine sample were collected during control period(saline infusion 30 ml/hr), period of indomethacin infusion(0.42 or 0.83 ㎎/min for 30 min. in saline) and recovery period for 45 min. Plasma clearance of various substances were obtained by measurements of plasma and urinary concentration of inulin, PAH, osmolarity, sodium, potassium, and chloride. The results are summarized as follows. 1. Urine flow rate during control period was 1.83±0.25 ml/10 min. 2. Urine flow rate during indomethacin infusion were 1.04±0.27(0.42 ㎎/min) and 0.90±0.18(0.83 ㎎/min) ml/10 min. Urine flow rate was reduced significantly (p<0.025) by infusion of indomethacin. 3. Renal plasma flow and glomerular filtration rate were decreased by infusion of indomethacin but they were not significant statistically. 4. Osmolar clearance and negative free water clearance decreased significantly (p<0.01) while osmolarity of urine increased by infusion of indomethacin. 5. Percent reabsorption of potassium in the renal tubule was decreased significantly (p<0.05) while any changes in sodium and chloride reabsorption were not observed by infusion of indomethacin. In the above results, it is suggested that indomethacin greatly potentiate the hycroosmotic action of vasopressin with decrease in medullary blood flow.
진철호(CH Jin),정지윤(JY Chung),김지근(JK Kim),김주옥(JO Kim),이상배(SB Lee) 대한산부인과학회 1988 Obstetrics & Gynecology Science Vol.31 No.1
저자들은 비교적 희귀한 난소의 기형종을 동반한 명세포암 1례를 경험하였기에 문헌적 고찰 과 함께 보고하는 바이다. Clear cell carcinoma of the ovary was described initially as being of mesonephric origin, but is thought to originate from the pluripotential surface epithelium of the ovary and to be a varient of the endometriod ovarian carcinoma. This clear cell carcinoma disclosed a high incindence of pelvic endometriosis. Survival rates are same as those of the other malignant ovarian tumors in stage I, but they are much lower than those in beyond stage I. A case of clear cell carcinoma with teratoma of the ovary in a 44 year old woman was presented and reviewed briefly.
원형빌렛으로부터 인볼루트 헬리컬 핀을 가진 제품의 비틀림 압출가공공정에 관한 연구
박대윤,진철호,진인태 釜慶大學校 1999 釜慶大學校 論文集 Vol.4 No.1
The twisting and extrusion process of the product with involute helical fin from the round billet is developed by the upper bound analysis. The twisting of extruded product is caused by the twisted die surface connecting the die entrance section and the die exit section linearly. In the analysis, the internal shear surface is defined as the curved twisted plane from the taisting of die surface and the shear work is calculated by the consumption of shear energy The increase rate of angular velocity is determined by the minimization of plastic work. The results of the analysis. It is shown that the angular velocity of the extruded product increases with the die twisting angle, the reduction of area, and decreases with the die length, the friction condition.
정지윤(JY Chung),진철호(CH Jin),김진근(JK Kim),이상배(SB Lee) 대한산부인과학회 1987 Obstetrics & Gynecology Science Vol.30 No.7
임신 39주의 25세된 초산모에서 제왕절개술로 분만된 흉복부유합 중복기형여아를 경험하였기에 문헌 고찰과 함께 보고하는 바이다 Conjoined twinning is a rare anomaly with an incidence estimated at 1 in 50,000 deliveries This paper presents a case of female thracoventropagus twin(dicephalus dipus dibrachius) delivered by cesarean section with brief review of Literature