RBL-2H3 세포에서 Spermine에 의한 히스타민 유리와 칼슘유입에 미치는 상백피의억제효과

채옥희,강경진,신인호,김영기,이무삼,전병득 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.2

Giuseppe Verdi의 오페라 무대의상 연구 : Concerning to La Traviata La Traviata 의 경우

현선희,김옥진 全南大學校家政科學硏究所 1998 生活科學硏究 Vol.8 No.-

The purpose of this study was to investigate how to express wearing style through characters and images of dramatis personae based on 「La Traviata」s played by 3 different directors : Mario Lanfranchi's performance, Franco Zeffirelli's performance and Richard Eyre's performance, with actual approach and costume expression of Opera by considering expressional characteristics of wearing style on Stage costume. First, stage costumes of each performance have the common expressional characteristics ; Silhouette shows historical setting, material and color shows characters and images of dramatis personae, details and trimming to make images more effective express characteristics ·social class ·personality ·images of the cast. As the plot progresses, main subject and atmosphere are expressed with darker color. It brings catastrophe. That means that even if each performance has been made in different year and by different director, whole atmosphere, color and materials of all stage costumes are closely similar. Second, stage costumes of the Opera has a tendency to be simplified around the late 20 century. Works of the middle 20 century use gay details and excessive trimming while works of the late 20 century use silhouette to express the historial setting and use the simple design but detail and trimming. Third, the recent stage costumes emphasize simple wearing style and symbolic representation of the cast including historical circumstances. That means unique clothes, stage costumes, follows simple and functional trend of daily dress. As the stage costume applies the popular trend, costumes style is getting simplified and adornment is getting disappeared. Modernism of the late 1900's might effect this trend. As conclusion, stage costume expression of Opera shows the current and the background which the symbol applies to and expresses characteristics of the cast. The stage costume plays a symbolic role in delivering the plot like dumb lines of Opera. That is, stage costume of composite art, Opera, plays a very important role as one of visual factors to elevate the atmosphere

옥수수 수염의 휘발성 성분

곽재진,이재곤,장희진,김옥찬 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.4

옥수수 수염에서 purge와 trap headspace 장치를 이용하여 분리된 휘발성 성분으로부터 표준품과의 mass spectrum 및 GC 머무름 시간의 비교에 의해 44종의 휘발성 성분을 확인하였다. 확인된 성분을 기능기별로 나누어 보면 alcohol 성분들이 9종, aldehyde와 ketone의 carbonyl 화합물이 7종, terpene 관련 화합물이 14종, pyrazine계 화합물이 3종, hydrocarbon 화합물 5종 및 기타 성분이 6종으로 나타났다. Peak의 면적비(%)에 의한 양적인 면을 비교하여 2-propanol(8.08%), pentanol(1.82%), hexanol(2.86%), heptanol(1.26%), nonanol(1.28%) 등의 비점이 낮고 분자량이 작은 alcohol 화합물, alcohol의 산화 생성물인 hexanal (3.68%), heptanal(7.40%), nonanal(7.93%), decanal(2.04%) 등의 aldehyde 화합물, limonene(1.68%), α-copaene(2.20%), β-caryophyllene(1.43%), β-selinene(1.03%), α-selinene(1.03%) 등의 terpene 관련 화합물과 methyl pyrazine(0.28%), 2,5-dimethyl pyrazine(0.09%), 2,6-dimethyl pyrazine(0.41%) 등의 pyrazine 화합물 등이 주요 향기성분으로 확인되었다. Volatile components of Cornsilk(Zea mays L.) were isolated by purge and trap headspace method and were analyzed by GC and GC/MSD. A total of 44 components were identified in the cornsilk volatile components including 9 alcohols, 7 aldehydes and ketones, 14 terpenes and terpene alcohols, 3 pyrazines, 5 hydrocarbons and 6 miscellaneous components. The major components were 2-propanol(8.08%), pentanol(1.82%), hexanol(2.86%), hexanal(3.68%), heptanal(7.40%), nonanal(7.93%), decanal(2.04%), α-copaene(2.20%), limonene(1.68%), α-selinene(1.03%), β-selinene(1.03%).

민간부문 건설사업관리 활성화방안

이정희,장홍진,구교진,김옥규,현창택,김양현 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

Since, korean government introduced Construction Management system by the law in 1997, only a few public construction projects have been adopted the system Nevertheless there are many laws which are related in public construction projects, Construction Management is positively carried out by non-government. About the activation plan of Construction Management questionnaire was inquired by experts in construction. The purpose of this study is to give a solution by finding out the problem of the laws, reconstructions remodelings.

吸光度測定에 대한 DDVP·Fenitrothion製劑의 分離定量에 관한 硏究

嚴東玉,金眞姬 又石大學校 1990 論文集 Vol.12 No.-

It is studied that the differential determination of DDVP in phosphate(combination of P=O) and Fenitrothion in thiophosphate(combination of P=S) by absorbance spectrophotometry. DDVP is determined by absorbance of colored reaction products that is produced to reaction of acetophenone under the basic condition and thiophosphate compounds is determined by absorbance of Pd·fenitrothion complex that is colored NDA. It examined the optimum reaction condition while DDVP reacted acetophenone under the basic condition and fenitrothion reacted Pd(Ⅱ)and NDA. It obtained maximum absorbance wave-length, it examined with regard to effect by acetophenone concentration, effect of concentration of PdCl_2, effect by concentration of NDA, effect of reaction temperature and heating time and effect of pH on absorbance of reaction products. Determination of DDVP and fenitrothion were possible to the range of DDVP 25-125㎍/㎖ and fenitrothion 1.0-5.0㎍/㎖. The analytical result were mean, 100.15 and standard deviation, 0.47 to DDVP, mean, 100.07 and standard deviation, 0.41 to fenitrothion. The method was applicable to the determination of DDVP and fenitrothion preparations.

노인 건강증진을 위한 운동프로그램의 적용 효과

안옥희,,남미라,서윤진,정은옥 노인간호학회 2005 노인간호학회지 Vol.7 No.2

Purpose: This study was to develop and to evaluate a health promotion program elderly. Method: This study was a quasi-experimental research. The participants were 62 elderly(experimental group:31, control group:31). Data were collected between September 10 and November 5, 2005. The data were analyzed by descriptive statistics, Independent t-test and ANCOVA. Result: 1. In designing the program, experimental group was given recreation program and pilates excercise 2 time a week, for 8 week. 2. Pilates excercise including recreation, significantly affected the BP, life satisfaction and depression. 3. Total cholesterol and blood sugar was not significantly affected by the pilates excercise. Conclusion: The Program was tested to promote the health of elderly and verified as an effective nursing intervention program, because the outcome of this program as certain that this program enhances life satisfaction, reduces depression and BP. Therefore this program is verified as a suitable exercise for the old people. The reason why firstly, there are no restricts of time and space. secondly, anyone can easily follow this as far as he can thirdly, this program is a soft and warm exercise.

TNF-α 유전자로 형질전환된 효모의 성장양태 및 단백질 생산

심두희,이진옥,이동근,이재화 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

사람 TNF-a를 생산하도록 GPD promoter와 AG promoter로 형질전환된 S. cerevisiae가 보이는 성장양상, 총분비단백질, pretease 활성을 측정하였다. GPD promoter로 형질전환 된yeast는 63.6 g/L의 성장이 일어났고 총분비단백질은 9 mg/L이며, pretease 활성은 2.55 X10^(4) unit/L 였다. AG promoter로 형질전환된 yeast의 경우엔 68 g/L의 성장이 측정되고 총분비단백질은 27 mg/L, pretease 활성1.55 X10^(4) unit/L였다. 단백질과 protease 분비량은 세포의 성장과 비례하는 것을 알 수 있었고, GPD promoter로 발현되는 TNF-a의 양은 배양초기에는 검출 한계 이하였고 15시간 이후에 증가하는 양상을 보여 배양 후 35시간째에 가장 많은 양 약 2535 ng/ml 정도가 분비되는 것으로 나타났다. We measured growth, total secreted protein and total secreted protease of transformed Saccharomyces cerevisiae with human TNF-α gene. Two different promoters, GPD promoter and AG promoter, were used for heterologous expression of human TNF-α gene. The growth which transformed TNF-α:GPD promoter was 63.9 g/L and total secreted protein was 9mg/L, protease activity was 2.55 x 10^(4) unit/L. In another case at TNF-α:AG promoter the growth was 68 g/L, total secreted protein was 27 mg/L and protease activity was 1.55 X 10^(4) unit/L. We could know total secreted protein and protease were directly proportional increase with growth. In initial time of culture, we could not see the TNF-α content in GPD promoter but after 15hours the yeast produced TNF-α. The highest level of product of TNF-a was 2535 ng/ml at 35hours after culture.

대구지역 실내 외 공기중 주요 대기오염물질의 농도조사에 관한 연구

최진수,백성옥,김영민,박상곤,정점희,황승만 嶺南大學校 環境問題硏究所 1995 環境硏究 Vol.15 No.1

최근 현대인의 실내거주시간이 일상생활시간의 80% 이상을 차지할 정도로 크게 증가하면서 보건·위생학적 측면에서 실내공기질은 매우 중요한 관심사로 등장하게 되었다. 본 연구는 1994년 8월의 여름철과 1994년 12월 ∼1995년 1월의 겨울철에 대구를 중심으로 가정집, 사무실, 식당과 같은 3가지 유형의 일반주거환경 중 실내·외 공기를 대상으로 실행되었다. 측정항목으로는 RSP, CO, CO₂, NO₂등의 주요 기준성 오염물질과 Bioaerosol을 선정하였다. 본 연구의 결과, 각 성분대상물질의 실내·외 농도는 실내거주환경 및 여름과 겨울, 두 계절에 따라 영향을 받는 것으로 나타났으며 실내·외 농도비교에서 RSP, CO, CO₂의 실내농도는 대부분의 지역에서 실외에 비하여 높은 농도를 나타냈다. 각 실내거주환경에 따른 실내·외의 농도는 가정, 사무실에 비하여 식당에서 상대적으로 높은 농도를 나타냈으며 실내·외 농도비 또한 식당에서 상대적으로 큰 값을 나타냈다. 여름과 겨울의 두 계절에 따른 RSP, CO, CO₂의 실내·외 농도는 여름철에 비하여 겨울철에 더욱 높은 농도를 나타냈으나 Bioaerosol의 실내·외 colony/plate는 여름철에 더욱 높은 수치를 나타냈다. The study was carried out to investigate the concentrations of indoor air pollutants such as respirable suspended particulate (RSP), carbon monoxide (CO), carbon dioxide (CO₂), nitrogen dioxide (NO₂) and bioaerosol at homes, offices and restaurants in Taegu city. Indoor and outdoor concentrations of these pollutants were observed simultaneously from August 1994 for summer to January 1995 for winter. The results were summarized as follows : 1. Both in summer and in winter, the indoor concentrations of RSP, CO, and CO₂; were higher than the outdoor concentrations, the indoor concentrations of major air pollutants (RSP, CO, CO₂, NO₂) in restaurants were higher than those in homes and offices. 2. The indoor and outdoor concentrations of RSP, CO, and CO₂; in winter were significantly higher than those in summer, on the other hand. The CFU/plate of bioaerosol in winter was lower than that in summer. These results suggested that indoor levels of air pollutants were affected by various indoor characteristics such as smoking, cooking, ventilation rate, winter heating systems, and behavioral activity of occupants.

백서 뇌의 정상 노화와 병적 노화 과정의 조직학적 차이 및 약물효과

전진숙,한호성,장희경,길영기,김순옥 대한생물치료정신의학회 1997 생물치료정신의학 Vol.3 No.1

Objectives : Besides of malnutrition and neurotoxic effect of alcohol on the prefrontal cortex or subcortical structures, premature aging may be involved in alcohol-induced cognitive disorders. The aims of this study were to evaluate the effect of alcohol on short-term memory function and histology, and to identify the drug responses and an association with aging process to understand a biological mechanism underlying alcoholic dementia. Methods: In experiment 1, T-maze tests were done in 5 aged controls and 5 atropine-treated rats. In experiment 2, T-maze tests were repeated on every week for a month in 5 normal adult and 5 ethanol-treated rats. In experiment 3, T-maze tests were repeated on every week for a month in seven groups of 5 ethanol-treated rats injected with normal saline, fluoxetine, bromocriptine, bethacholine, nimodipine, clonidine and ketamine. After completion of behavioral tests rats were sacrificed by the intracardiac perfusion with phosphate buffered 10% formaldehyde solution, and the brain specimen was stained with hematoxylin-eosin to count cells in prefrontal cortex and hippocampus. Results: 1) Cell numbers of hippocampus(CA1, CA3 and dentate gyrus) and prefrontal cortex were reduced in ethanol-treated rats(P<0.05) without significant changes on T-maze tests. 2)Cell numbers of hippocampus(CA1, CA3 and dentate gyrus) and prefrontal cortex were recovered by bethacholine(P<0.05), while those of hippocampus raised by bromocriptine and clonidine(P<0.05 respectively). There were no significant changes on T-maze tests. 3) Cell numbers of prefrontal cortex in ethanol-treated rats were correlated with those of atropine-treated(r=0.977,P<0.001), and of normal aged(r=0.448, P<0.05) rats. Conclusions : Alcohol-induced memory disorder might be mainly related with cholinergic system as well as adrenergic or dopaminergic ones. Pathological aging process could be involved in a mechanism underlying alcoholic dementia.

지하철 출입구 주변의 어메니티 개선에 관한 연구 : A Case Study of the Pusan Subway 1st Line 부산시 지하철 1호선을 중심으로

도창희,옥진아,김승환 東亞大學校生命資源科學大學附設 農業資源硏究所 1997 農業生命資援硏究 Vol.6 No.1

This study covers amenity analysis of the subway 1st line and improvements focusing on the easiness, amenity, safeties, and elements of amenity. When people come to the entrance of a subway station, they pay careful attention to easiness of use, amenity and locality of the area. Therefore, by getting rid of unnecessary stairways, we can minimize the economical loss secure the easiness of use and stability. At the same time, well designed planters and trees dedicated to boulevards which can increase the ratio of green space. Furthermore, each gate of subway stations should have the identity of the area. Finally, we suggest those facts which are mentioned above should not be repeated that when designing the Pusan subway line 2nd line which is under construction.