Protein kinase A-induced phosphorylation at the Thr154 affects stability of DJ-1

Ko, Yeon Uk,Kim, Su-Jeong,Lee, Juhyung,Song, Min-Young,Park, Kang-Sik,Park, Jun Bae,Cho, Hyun-Soo,Oh, Young J. Elsevier 2019 Parkinsonism & related disorders Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Most cases of Parkinson's disease (PD) are sporadic, but genetic variations have been discovered in PD patients. PARK7/DJ-1 is a known cause of early-onset autosomal-recessive PD and is implicated in neuroprotection against oxidative stress. Although several post-translational modifications of DJ-1 have been proposed, phospho-modification of DJ-1 and its functional consequences have been less studied.</P> <P><B>Methods</B></P> <P>Putative phosphorylation sites of DJ-1 were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS analysis). Subsequently, phosphorylation site of DJ-1 was confirmed by <I>in vitro</I> kinase assay and cell-based pull-down assay. Impaired dimer formation of phospho-null mutant was measured using DSS crosslinking assay and immunoprecipitation assay. To evaluate physiological consequences of this event, protein stability of DJ-1 WT and DJ-1 phospho-null mutant were compared using cycloheximide chase assay and ubiquitination assay.</P> <P><B>Results</B></P> <P>Here, we showed that DJ-1 directly bound to the catalytic subunit of protein kinase A (PKAcα). We found that PKAcα is responsible for phosphorylation of DJ-1 at the T154 residue. Interestingly, dimerization of DJ-1 was not detected in a DJ-1 T154A mutant. Furthermore, stability of the DJ-1 T154A mutant was dramatically reduced compared with that of wild-type DJ-1. We found that DJ-1 T154A was prone to degradation by the ubiquitin proteasome system (UPS).</P> <P><B>Conclusion</B></P> <P>We identified a novel phosphorylation site of DJ-1. Furthermore, we determined protein kinase A that is responsible for this posttranslational modification. Finally, we demonstrated physiological consequences of this event focusing on dimerization and protein stability of DJ-1.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PKAcα phosphorylates PARK7/DJ1 at the Thr154 residue. </LI> <LI> Disruption of Thr154 phosphorylation is linked to a lower stability. </LI> </UL> </P>

이중형질전환에 의한 웅성불임유기유전자의 발현억제

박영두,김현욱,박범석,진용문,정태영 慶熙大學校 1997 論文集 Vol.26 No.-

This study was carried out with the purpose of looking into the regulation of gene related to anther development, and applying their promoter to induce male sterile plants and restore their fertility. Progenies of transgenic male sterile tobacco plants containing anther specific promoter (BAN215) and diphteria toxin A (DTx-A) gene were analyzed by danamycin screening and pollen observation. DTx-A gene in male sterile plants was confirmed by PCR with the two synthetic primers, 5'-AACTTTTCTTCGTACCAC-3' and 5'-GCTTTCGCCTGTTCCCAG-3', from the sequence of DTx-A genes. The fragment of 350bp was appeared when the PCR product of 428 bp was digested with Rsal. pDTx-2 containing DTx-A gene was used as a control (DTx). Some of them were double transformed with the construct of BAN215 promoter and antisense DTxA (pKDA215b) to test restoration of fertility.

보건관리 대행사업 평가도구의 타당성, 신뢰도 및 인과관계 검증에 관한 연구

박정일,조병희,구정완,김광종,김현욱,원종욱,이명숙,정문희 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.1

In order to verify the validity, reliability and relativeness of preliminary evaluation tool for the group occupational health services(GOHS), a questionnaire survey for doctors, nurses and hygienists of 18 GOSH institutions, and employers, health managers and employees of 340 enterprises was conducted. A factor analysis and correlation test for the statistical validity of the variables in the evaluation tool, a Chronbach's Alpha value for the reliability in the evaluation indices and a regression analysis for the relations of the evaluation model were performed. The results were as follows: 1.In factor analysis, thirty-three variables among 43 variables were selected as significant variables, ie 9 out of 11 for input index, 11 out of 14 for throughout index and 13 out of 18 for out put index. 2.Three evaluation(outcome) scales(evaluation by employer, health manager and employee) which composed of 16 identical items were used. 3.The Chronbach's Alpha values of input, throughout, out put and outcome indices were 0.5221, 0.5473, 0.7122 and 0.8546 respectively. 4.Regression equations of independent variables on the evaluation scales of employer, health manager and employee were all statistically significant.

스포츠 사회학적 관점으로 본 한국문학의 형태 연구 : 1990년대 전·후 스포츠 소설을 중심으로

박현욱 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study has examined the value and historical significance of sport novels that began to occur from late 1970's, and reviewed the state of sport literature in Korea comparing and analyzing it with foreign sports literature. Then we have analyzed comprehensively the characteristics of characters, structural properties and themes appearing in sports novels written around 1990's. The results are as follows. 1. Observing the sports novel in prior and post 1990's era from a literary social view, it directly reflects the periodic state of the written era along with the sports form, 2. Studying the common personalities of characters appeared in prior and post 1990's era, it shows the sportsmanship such as the sacrificial spirit and poverty for others, harsh training and combativeness, strict victory or defeat, love, and an attitude of giving the utmost best overcoming the difficulty and depression of which sublimates the natural environment of sports appeared in the sports novel of USA and Japan. 3. Looking into the structural characteristics of the sports novel in prior and post 1990's era, it appropriately introduces of the foreshadow and struggle of the long novels of which by using the complex and dimensional structure method as in sports literature of Japan and USA, the literary artistic side and prfessionalism of sports are well expressed therein. 4. The sports novels of prior and post 1990's era, as through the lifes scale-down called the sport, clearly reveas the authors prfound subjective identity in trying to overcome the modern societys structural hypocrisy and restriction along with the nature of humans dreaming about the self-realization. Therefore, it is seen that the Japan and USA sports novels projected respectable sportsmanship and the serious self-examination on life are included in them.

호스트기반 라우팅을 이용한 마이크로 이동성 관리

朴昡旭,安弘榮 弘益大學校 科學技術大學 大學院, 2004 産業技術 Vol.14 No.-

Mobile IP provides convenient and extensible global mobility management protocol. However It does not support fast Handoff and Paging. Local mobility management protocols like Cellular IP, HAWAI and Hierarchical Mobile IP are proposed to support frequent Handoff in intra IP domain. In this paper, we propose a new host-based routing algorithm which maximizes the advantage of extra links using controlled flooding. Simulation results show that a new routing algorithm has a better performance in packet loss and throughput metric.

양궁선수의 경쟁불안 수준에 대한 조사 연구

박현욱,윤혜영 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The research had a conclusion as a result of investigation that is competitive anxiety level before match each sex, capacity, object, religion about 123 players of middle school, high school, university students who particiate in the whole country tournament by 2003. First, as a result of research that is competitive anxiety level each sex, a somatic state anxiety of low grade factor is inspected alike in 1% level statistically. And women(M=1.1846) are inspected higher than man (M=1.0590). In a state self-confidence, it is inspected alike much in 1% level statistically, and man(M=3.8733) are inspected higher than women (M=3.7589). But in a cognitive state anxiety, alike contrast is not inspected. Second, in a each capacity level, cognitive state anxiety was inspected alike much in 1% level statistically, superiority group (M=1.082) is higher than inferiority group(M=1.043). But, there wasn't similar gap in somatic state anxiety and state self-confidence. Third, at each archer's competitive anxiety level, we can find statistically 1% similar gap in cognitive state anxiety. and the result of Duncan's multiplex range test showed that There was wide difference of averages between university students and high school, between university students and middle school students, between high school students and middle school students. but in somatic state anxiety and state self-confidence, there wasn't similar gap statistically. Fourth, The research of archer's competitive anxiety level on a basis of religion said that cognitive state anxiety and state self-confidence was proved to have some gap about 1% statistically, and the level of cognitive state anxiety was most high in the group of none religious and the next was buddism, catholic, christian, the state self-confidence of none religious group was higher than that of Christian group, but There wasn't similar gap in somatic state anxiety level on a basis of religion.

한약재의 중금속 평가 연구 : 경북북부지역 한약재를 중심으로 Cultivated Herbal Medicines at North Gyeongbuk Area

박문기,김승영,황현욱 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

We compared with heavy metal concentration of herbal medicines in products of Gyeongbuk and the other area of Korea. The concentration of heavy metal were studied for the estimation of quality in herbal medicines which is Bupleuri Radix, Paeoniae Radix, Dioscoreae Rhizoma and Astragali Radix in products of north Gyeongbuk area. The average levels of heavy metal of herbal medicines in Gyeongbuk area are as follows : Mercury is 0.037mg/kg, Chromium is 0.093mg/kg, Nickel is 0.108mg/kg, Copper is 0.475mg/kg, Zinc is 3.14mg/kg, Manganese is 1.52mg/kg, Iron is 7.83mg/kg, and Cadmium, Lead and Arsenic is not detected. It was very lower in concentrations than those of average Korea area. Therefore, Gyeongbuk area appear to be more proper to cultivated land than other area. Minerals such as Ca and K were extracted more than Na and P in herbal medicines products of Gyeongbuk respectively. Minerals and heavy metal concentrations distributed in herbal medicines were analyzed. Little or no relationship was observed between minerals and heavy metals.

복합레진의 지연충전이 미세인장 결합강도에 미치는 영향

박현식,조영곤,박병철,김종욱,최희영,김종진,진철희,유상훈,기영재 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.3

The purpose of this study was to evaluate the effect of immediate or delayed composite resin filling on dentinal microtensile bond strength (μTBS) after applied the adhesive. The coronal dentin of human third molars was exposed. Single-Bond or One-Step was applied on the dentin surfaces, and composite resin were constructed immediately (group 1) or 5 min., 10 min., 15 min., 20 min. and 30 min. (groups 2-6) after an adhesive was applied. The specimens were sectioned and made bar-shaped. Each surface area of them was about l ㎟. The RBS test was performed by EZ test. The results were analysed by One-way ANOVA and Tukey's test at 95 % significance level. The results suggested that the μTBS of Single-Bond to dentin was decreased when the composite resin was constructed 20 min. and 30 min. after Single-Bond was applied. But the μTBS of One-Step was not affected by delayed composite resin filling.

초기 성공적인 심폐소생술후 혈청 Interleukin-6 농도의 발현양상

박규남,박승현,황두영,김형국,이환,최경호,이원재,정욱성,김학주,김세경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Objective : To investgate patterns of serial serum interleukin-6(IL-6) concentrations after initial successful cardiopulmonary resuscitation, and to access differences in serum IL-6 concentrations in patients with Cerebral Performance Category(CPC, 1-2) and CPC(3-5) at the 5th day after cardiacpulmonary resuscitation(CPR). Methods : We studied prospectively 12 patients with spontaneous circulation after cardiopulmonary resuscitation. Venous blood samples were taken 2, 4, 12, 24, 48 and 72 hours after restoration of spontaneous circulation to measure serum IL-6 levels by the enzyme-linked immunosorbent assay. Results : Serum IL-6 concentrations in the severe neurolgically disabled group(CPC, 3-5) were significantly increased at 2, 24 and 48 hours after restoration of spontaneous circulation compared with the less neurolgically disabled group. Also, serum IL-6 concentrations in the severe neurolgic disabled group showed dual peak at 4 and 24 hours. Conclusion : Our study demonstrates significant increase of serum IL-6 in patients with CPC(3-5) after successful cardiopulmonary resuscitation and the significant correlation between early increase of serum IL-6 and poor neurologic outcome. It suggests that early increase of serum IL-6 can be used as a prognostic marker after successful cardiopulmonary resuscitation.

리스페리돈과 올란자핀이 투여된 정신과 환자들에서 간 효소 변화에 대한 후?적 연구

임현국,배치운,박석준,김정진,이창욱,이수정,이철,백인호 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.3