Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

초등학교 교사들의 직무스트레스와 건강 수준

조현숙,이종태,손혜숙,박진국 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objective : The purpose of this study was to assess the effect of job strain on stress and health status among elementary school teachers. Methods and Material : The subjects in this study were 300 teachers who were selected from among 588 teachers in 13 out of 42 elementary schools in the city of Gimhae. Through the questionnaire, demographic characteristics, Karasek's Job Content Questionnaire(JCQ) and the Psychosocial Well-being Index (PWI) to assess their occupational characteristics and psychosocial stress level were surveyed. Medical checkup results of them were adopted to measure their Health status. Results : 24.9 percent of the general teachers were low strain group, and 16.3 percent of them were high strain group. In the senior teachers, 19.7 percent belonged to a low strain group and 21.3 percent belonged to a high strain group. In regard to health status, more senior teachers than junior teachers suffered from obesity(24.6%) and diabetes(11.5%) (p<0.O5), and they also were higher in DBP(p<0.057), total cholesterol(p=0.039) and blood sugar level(p=0.000), the low strain group perceived more social support(p=0.000), and the high strain group felt bigger physical overload(p=0.000). Concerning psychosocial stress, the low strain group was more aware of teacher social role and put more confidence in themselves(p=0.004). The high strain group was more depressed(p=0.001), but no difference was detected in health status. The health status difference between the high strain and low strain groups was significant in depression of psychosocial stress(OR=3.823, 95%CI=1.584-9.224). Conclusion : The senior teachers group was more morbid to obesity, diabetes and hyperlipidemia, which implies that some measure should be studied to help senior teachers manage those diseases properly. The high strain group suffered more from physical overload and was high 'Social support'. Therefore it's required for high strain group to be given more support from colleagues and supervisors, and a stress management program should be implemented to reduce their psychosocial stress.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

파노라마방사선사진 지수와 임플란트 실패와의 관계에 관한 연구

조현정,이원진,허민석,안창현,이진구,이삼선,최순철 大韓口腔顎顔面 放射線學會 2004 Imaging Science in Dentistry Vol.34 No.1

Purpose : Several panoramic indices have been suggested to assess bone quality from the morphology and width of mandibular cortex on panoramic radiography. The purpose of this study was to compare dental implant failure group with control group in panoramic mandibular index (PMI), mandibular cortical index (MCI), and gonion index (GI) and to determine the effect of these panoramic indices on dental implant failure. Materials and Methods : A case-control study was designed. Test group (n = 42) consisted of the patients who had their implants extracted because of peri-implantitis. Control group (n = 139) consisted of the patients who retained their implants over one year without any pathologic changes and had been followed up periodically. They had dental implants installed in their mandibles without bone augmentation surgery from 1991 to 2001. The following measures were collected for each patients: 1) PMI, MCI, and GI were measured twice at one-week interval on preoperative panoramic views; and 2) age, sex, implant length, implant type, installed location, occluding dentition state, and complication were investigated from the chart record. Results : The PMI showed moderate level of repeatability. The intra-observer agreement of MCI and GI were good. There was statistically significant difference in PMI between two groups. There were significant different patterns of distribution of MCI and GI between two groups. Among the panoramic indices, PMI and MCI showed significant correlation with dental implant failure. Conclusion : Panoramic indices can be used as reference data in estimating bone quality of edentulous patients who are to have implants installed in their mandibles.

엘도스 캡슐(에르도스테인 300mg)에 대한 엘브론 캡슐의 생물학적 동등성

조혜영,이석,강현아,문재동,이용복 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

Erdosteine, the thiol derivatives chemically related to cysteine, is a mucolytic and mucoregulator agent which modulated mucus production and viscosity and increases mucoiliary transport. The purpose of the present study was to evaluate the bioequivalence of two erdosteine capsules, Erdos (Dae Woong Pharmaceutical Co., Korea) and Erblon (Kuhn Ⅱ Pharmaceutical Co., Korea), according to the guidelines of Korea Food and Drug Administration (KFDA). The erdosteine release from the two erodisteine capsules in vitro was tested using KP Ⅷ Apparatus Ⅱ method with various different kinds of dissolution media (pH 1.2, 4.0, 6.8 buffer solution and water). Twenty four healthy male subject, 23.33±2.06 years in age and 66.18±8.19 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After three capsules containing 300 ㎎ as erdostein were orally administered, blood was taken at predetermined time intervals and the concentrations of erdostein in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using log-arithmically transformed AUC_(t) and C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Erdos were 0.20%, 1.10% and -9.44% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of long(0.8) to log(1.25)(e.g., long(0.94)∼long(1.22) and log(0.92)∼log(1.20) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Erblon capsule and Erdos capsule are bioequivalent.

서비스의 개념과 척도에 관한 연구

조현철,강석후 漢陽大學校 産業經營硏究所 1992 産業經營硏究 Vol.4 No.-

개구리(Rana nigromaculata)胃 粘液質의 組織化學的 硏究

趙顯旭,朴重錫,河在淸 慶尙大學校 1985 論文集 Vol.24 No.2

개구리 위 점막내에 존재하는 점액질의 성분을 변태 시기에 따라서 조직화학적으로 관찰하였다. 원주세포 내 점액질은 변태 XVI와 XXIV 시기에는 바황화점액질로 된 산성점액질에 비해서 중성점액질이 주를 이루었고 변태 XX 시기에서는 산성 및 중성점액질이 혼재하였다. 점액선 세포내 점액질은 관찰된 변태 세 시기에서는 주로 중성점액질로 되어 있었다 This study was-performed histochemically to observe the responses of mucus secreting cells in the gastric mucosa of frog, Rana nigromaculata during metamorphosis. The obtained results were as follows: 1. Columnar cells contained a small amount of acidic mucosubstances including nonsulfated. mucin and a large amount of neutral mucosubstances at XVI and XXIV stages of metamorphosis. At XX stage, columnar cells contained acidic and neutral mucosubstances. 2. Mucous glands contained mainly neutral mucosubstances at XVI, XX and XXIV stages.

瓦松의 알콜 抽出物이 心筋 및 腸筋收縮에 미치는 影響

曺石鉉,金光鎭 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Orostachys malacophyllus F. is a kind of ,Chinese medicine to grow on the tiled roof of the house or on the rock of the mountain. The chemical compositions and actions have not been reported, but, sometimes, it was used as a folk remedy for the treatment of cancerous diseases. This study was attempted to investigate the effects of water fraction separated from alcohol extract of Orostachys malacophyllus F. on the contractilities of cardiac and intestinal muscles comparing with those of the water extract. The muscles used for this study were the ventricular muscle of turtle and the intestinal muscle of mouse, and their contractilities were represented as a change of the muscle tension detected by myograph. The results were summarized as follows: 1. The water fraction decreased the contractility of ventricular muscle, and increased the intestinal contractility. But the water extract was observed the opposite effects on the contractility of each muscle. 2. Sympathetic or parasympathetic blocker and alpha-or beta-blocker had no effect on the decreased or increased contractility of each muscle. 3. Calcium antagonist(verapamil) inhibited significantly the contractility in the case of the water fraction to the intestinal muscle and the water extract to the ventricular muscle. From the above results, it was suggested that the action of water fraction or the water extact affected to calcium transport: release into the sarcoplasmic fluid or active influx into the sarcoplasmic reticulum in the case of the cardiac muscle, and release from ECF or cisternae of reticulum into the muscle cell in the smooth muscle.

아마릴 정(글리메피리드 2㎎)에 대한 글리메드 정의 생물학적 동등성

조혜영,박은자,강현아,백승희,이석,김세미,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

The purpose of the present study was to evaluate the bioequivalence of two glimepiride tables, Amaryl^(?)(Handok/Aventis Pharm. Co., Ltd.) and Glimed (Kuhn Ⅱ Pharm. Co., Ltd.), according to the guidelines of Korea Food and Drug Administration (KFDA). The glimepiride release from the two glimepiride formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with a variety of dissolution media (pH 1.2, 4.0, 6.8 buffer solution, water and blend of PSB 80 into each dissolution medium). Twenty six healthy male subjects, 22.65±2.19 years in age and 66.55±8.85 kg in body weight, were divided into two groups and randomized 2×2 cross-over study was employed. After one tablet containing 2 ㎎ as glimepiride was orally administered, blood was taken at predetermined time intervals and the concentrations of glimepiride in serum were determined using HPLC method with UV detctor. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Amaryl were -3.70, -8.28 and 0.61% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25)(e.g., log(0.84)∼log(1.04) and log(0.82)∼log(1.03) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guideline for the bioequivalence were satisfied, indicating Glimed tablet and Amaryl tablet were bioequivalent.

리스페달 정(리스페리돈 2㎎)에 대한 리스펜 정의 생물학적 동등성

조혜영,박은자,강현아,백승희,이석,박찬호,문재동,이용복 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.2

The purpose of the present study was to evaluate the bioequivalence of two risperidone tablets, Risperdal (Janssen Korea Co., Ltd) and Rispen (Myung In Pharm. Co., Ltd), according to the guidelines of Korea Food and Drug Administration (KFDA). The risperione release from the two risperidone formulations in vitro was tested using KP Ⅷ Apparatus Ⅱ method with various of dissolution media (pH 1.2, 4.0, 6.8 butter solution and water). Twenty four healthy male subjects, 23.33±2.10 years in age and 69.24±8.05 kg in body weight, were divided into two groups and a randomized 2×2 crossover study was employed. After one tablet containing 2 ㎎ as risperidone was orally administered, blood was taken at predetermined time intervals and the concentration of risperidone in serum were determined using HPLC method with UV detector. The dissolution profiles of two formulations were similar at all dissolution media. Besides, the pharmacokinetic parameters such as AUC_(t), C_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t), C_(max) and untransformed T_(max). The results showed that the differences between two formulations based on the Risperdal were 0.20, -1.29 and -11.09% for AUC_(t), C_(max) and T_(max), respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.25) (e.g., log(0.90)∼log(1.03) and log(0.84)∼log(1.09) for AUC_(t) and C_(max), respectively). Thus, the criteria of the KFDA guideline for the bioequivalence were satisfied, indicating Rispen tablet and Risperdal tablet were bioequivalent.