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RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
유한 요소법을 이용한 중소하천 유역에서의 이동호우에 대한 유출특성 분석
조현경,이영화,최윤영 한국환경과학회 1998 한국환경과학회지 Vol.7 No.4
In the rainfall-runoff relation, consideration of the spatial movement of storms is very important in designing a hydraulic structure or evaluating an environmental influence for land usage. Because of this reason, this study has suggested the finite element model which consider the spatial movement of a stome and it was applied on a small river basin(Wi stream basin). In the application of the model the basin was treated as a pivot point and the storms are simulated with movement in each directions. As a result, it shows that the storms moving from north to south have bigger peak discharge and faster peak time than the storms moving in other directions. So these characteristics have to be considered in the designation of a hydraulic structure or evaluation of an environmental influence.
S.D. Rats를 이용 1, 1-Dichloro-1-fluoroethane의 아민성 흡입독성 연구
김현영,이성배,임철홍,김철우,점용현,한정희,전윤석,최수영,강대봉,이용묵 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1
There were no specific effects for test materials on Sprague-Dawley (S.D.) rats in clinical symptoms, amounts of food intakes, weight changes, laboratory findings, and pathology after whole body l, l-Dichloro-1 -fluoroethane (used as coolant, metal cleaner and solvents) exposure(0, 1,500, 3,000, and 6,000 ppm) for 13 weeks (6 hour/day, 5 days/week). However, the loss of capillary vessels in eyeball (pupil) was observed in a female rat among 6,000 ppm group. Though there was a tendency for MCHC (Mean Corpuscular Hemoglobin Concentration) in rat to be decreased (p<0.05), it was not regarded as abnormal because the values were within normal limits. In asthma-stimulation related evaluations, there was also a tendency for inflammatory cell counts in bronchoalveolar lavages to be increased. But it had no statistical significance, and also no dependency on sex and the exposed concentration. Based on this result, the non observed effect level (NOEL) induced by 1, 1-Dichloro-l -fluoroethene inhalation was evaluated in groups with 3,000 ppm below (S .D. Rats, 13 weeks). Finally, it was concluded that the short term exposal of 1, 1-Dichloro-l-fluoroethane is not considered as a asthma stimulant by inhalation despite of some study limitations such as test animals use and short-term exposure .
다발성 골수종에서 저용량 thalidomide, cyclophosphamide, dexamethasone (TCD) 요법의 효과
류충헌,정재현,고정해,장제혁,박영진,최규남,박봉수,이상민,주영돈 인제대학교 2008 仁濟醫學 Vol.29 No.-
Background and Objectives : The immunomodulatory drug thalidomide can inhibit angiogenesis and induce apoptosis in experimental models. It can also induce marked and durable response in newly diagnosed myeloma patients. Thalidomide has been used at doses ranging from 200 to 800 mg with significant toxicity. No data are available on the impact of low-dose thalidomide, cyclophosphamide and dexamethasone as initial therapy for myeloma patients. Design and Methods : To address this issue, newly diagnosed myeloma patients were treated with 50 mg/day thalidomide continuously and cyclophosphamide 150 mg/m², days 1-4 and dexamethasone 20 mg/m², days 1-5 and day 15-19, every month. Between October 2005 and October 2006, 14 patients (median age 54.5 years) were treated with low-dose thalidomide, cyclophosphamide and dexamethasone. Results : After a minimum of two cycles of treatment, 5 patients (55.5%) showed a partial remission. After four cycles of treatment, 10 patients (83.3%) showed a partial remission (n=6) and complete remission (n=4). After a median follow-up of 15.4 months, 1 year overall survival rate was 82.0%. Thalidomide was well tolerated without serious toxic effects. Conclusions : The combination of low-dose thalidomide, cyclophosphamide and dexamethasone demonstrates favorable response rate and 1 year overall survival rate in newly diagnosed myeloma. Severe toxicities were not seen with this combination.
Yeong-Hyeon Choi,Kyu-Hye Lee 복식문화학회 2020 服飾文化硏究 Vol.28 No.2
This study aimed to analyze the performance of Disney-collaborated fashion lines based on online consumer reviews. To do so, the researchers employed text mining and network analysis to identify key words in the reviews of these products. Blogs, internet cafes, and web documents provided by Naver, Daum, and YoutTube were selected as subjects for the analysis. The analysis period was limited to one year after for the 2019. Data collection and analysis were conducted using Python 3.7, Textom, and NodeXL. The research terms in question were as follows: ‘Disney fashion collaboration’ and ‘Frozen fashion collaboration’. Preliminary survey results indicated that ‘Elsa’s dress’ was the most frequently mentioned term and that the domestic fashion brand Eland Retail was the most active in selling Disney branded clothing through its own brand. The writers of reviews for Disney-collaborated fashion products were primarily mothers with daughters. Their decision to purchase these products was based upon the following factors; price, size, stability of decoration, shipping, laundry, and retailer. The motives for purchasing the product were the positive response of the consumer’s child and the satisfaction of the parents due to the child’s response. The problems to be solved included insufficient quantity of supply, delay in delivery, expensive price considering the number of times children’s clothes are worn, poor glitter decoration, faded color, contamination from laundry, and undesirable smells immediately after the purchase.
보안성이 강화된 무선 공유기 개발에 관한 연구 - 공공장소와 홈 네트워크를 중심으로 -
최가영(Choi, Ga Yeong),김지현(Kim, Ji Hyeon),박지현(Park, Ji Hyeon),이진아(Lee, Jin-A),최다혜(Choe, Da Hye),손종모(Sohn, Jong Mo) 한국IT서비스학회 2019 한국IT서비스학회 학술대회 논문집 Vol.2019 No.2
개인용 IT 기기의 보급률이 높아짐에 따라 무선 네트워크에 대한 수요는 굉장히 높은 수준을 유지하고 있다. 따라서 어떤 장소에 방문해도 편리하게 무선 네트워크를 사용할 수 있도록, 우리나라 대다수의 장소에 무선 공유기가 설치되어 있다. 하지만 이들 공유기는 대부분 보안적인 측면에서 전혀 관리되고 있지 않으며, 공유기 관리용 웹사이트 역시 방치되는 경우가 많다. 이렇듯 위험성이 높은 현 상황의 무선 공유기 관리에 대해 개선점을 찾고자 본 논문에서는 무선 공유기로서 라즈베리파이3를 AP모드로 설정한 후, 방화벽과 침입탐지차단시스템을 이용해 보안 기능이 탑재된 공유기를 개발하는 연구를 진행하였다. 이 때 사용자 중심의 무선 공유기 관리용 모바일 애플리케이션을 함께 개발하여, 누구나 쉽게 무선 공유기의 정보를 확인하고 관리할 수 있도록 서비스를 제공할 방법을 모색하였다.
영화 의상에 나타난 사회 계급의 표현 -봉준호 감독의 <기생충>을 중심으로-
최영현 ( Yeong-hyeon Choi ),이규혜 ( Kyu-hye Lee ) 한국의류학회 2020 한국의류학회지 Vol.44 No.5
This study examines how class differences are expressed through costumes based on the costumes of characters in Bong Joon-Ho's film < Parasite >. Based on Video on Demand (VOD), the main situations in which characters' costumes change in the play were captured and used as analysis data. Colors, textures, and color symbol were analyzed to find the formative properties shown in the characters' costumes. The results are as follows. The home wear of Kim's family were unsuitable for the top and bottom, faded clothes, and the vague boundary between outdoor and indoor clothes appeared. In comparison, Park's family's home wear was featured a modern and elegant design, a clear distinction that suited purpose and situation. Analysis of street wear showed that the Kim's family had a big difference before and after getting a job, and Park's family wore different costumes for the purpose of going out. Social class expressed in the film costumes were shown to have the characteristics of intrinsic class invariance, temporary class changes, differences in class expression by age, and differences in costume choice by class.