韓國人에서 重合酵素反應으로 分析한 DXS52 遺傳座位의 遺傳的 多樣性

황적준,박희경,이경,이희석,송은섭 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The DXS52 locus was studied in a Korean population sample(n=142) for its allele and genotype frequencies and applicability to identity and paternity testing, using polymerase chain reaction with electrophoresis of PCR products and subsequent detection of allelic fragments by ethidium bromide staining. In Korean population, 15 alleles with the frequency range of 0.319-0.005 are detected in the DXS52 locus, and the highest allele frequency among them is found to be allele 1(0.319). The correlation coefficient between gene frequencies detected in female in mali is 0.9334, indicating that the gene frequency in males follows that of females in Korean population. This locus studied meets Hardy-Weinberg expectation : there is good agreement between the observed and expected values under Hardy-Weinberg equilibrium. The genotypes 1-12(0.118) are mostly frequent found among the 29 genotypes detected in Korean female, but the 91 genotypes are not found. The observed heterozygosity for this locus is 0.809, while the unbiased expected estimate of heterozygosity is 0.827 calculated by the expectation of Hardy-Weinberg equilibrium(p>0.05). The power of discrimination(PD) and power of exclusion(PEX) calculated for this locus is 0.9770(97.70%) and is 0.7068(70.67%), respectively. Thus, the allelic and genotypic data estimated for DXS52 locus can be used as the database of PCR-based DNA profile to human identity testing.

표백된 법랑질의 표면처리방법에 따른 복합레진과의 전단결합강도에 관한 연구

황호길,김은희,조영곤 大韓齒科保存學會 1995 Restorative Dentistry & Endodontics Vol.20 No.1

평양 남정리 53호분 출토 토제칠기의 보존 및 제작기법 연구 : 토제칠이배, 토제칠반, 토제칠안

황현성,임수경,김희진,이태진,고민정,윤은영 국립중앙박물관 2013 고고학지 Vol.19 No.-

본고는 일제 강점기 조사 미등록 유물 정리사업의 일환으로 수행한 평양 남정리 53호분 출토 토제칠이배, 토제칠반, 토제칠안에 관한 연구이다. 남정리 출토 유물은 소지가 목심이 아닌 토제라는 것과 칠이배와 칠반을 얹은 칠안이 함께 출토되었다는 점에서 주목할 만하다. 토제칠이배 및 칠반 그리고 칠안의 보존처리를 통해 이들의 형태와 문양 등에 대해 알 수 있었다. 또한 칠기에 사용된 붉은색 안료의 성분을 분석하여 안료의 주성분이 진사(HgS)였음을 확인하였다. 이번 연구에서 기존에 잘 알려지지 않았던 한반도 내 위치한 낙랑의 토제칠기에 관해 살펴볼 수 있었으며 이러한 제작방법이 한나라 고유의 문화인지 아니면 재지세력이 한나라의 영향을 받아 현지화한 문화의 산물인지에 대해 생각해 볼 수 있는 기회라 생각된다. This research paper examines the lacquered pottery cup, plate and table unearthed from Tomb No. 53 at Namjeong-ri in Pyongyang, which were under conservation treatment as a part of the Project of Unregistered Artefacts Uncovered in the Japanese Colonial Era. It is very rare to have a lacquered pottery cup and plate, lying on a lacquered pottery table, as a set of grave goods. In particular, they are not wooden but pottery items. Through conservation treatment for lacquered pottery cup, plate and table, it was possible to find out their shapes and decoration patterns. The resulting analysis of the red pigment used indicates that cinnabar (HgS) is the main element. This research is significant because it examines lacquered pottery wares produced in Lelang, which has rarely been conducted. Further research will need to prove whether their production techniques were in the unique Chinese Han style or were produced by indigenous craftsmen who adopted the Chinese Han technique.

셀룰로오스, 펙틴 및 알긴산 수준이 흰쥐의 혈청지질 농도에 미치는 영향

황은희,서은숙,이형자 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.1

This study was carried out to investigate the effects of the kinds and the amounts of dietary fiber on serum lipid composition in rats. Seventy-two male Sprague-Dawley rats weighting 160±10g were fed diets containing 1.0%, 3.6%, 6.0% and 10.0% α-cellulose, pectin and alginic acid for 4 weeks. Daily food intake of experimental groups was significantly higher in the α-cellulose 10.0% group than in the other groups. Increasing the dietary fiber level decreased the food efficiency ratio of the α-cellulose, pectin and alginic acid groups. Serum triglyceride concentration was significantly lower in the rats fed the alginic acid diet. Serum total cholesterol concentration showed no significant difference in the kind and the amount of three dietary fibers. Serum HDL-cholesterol concentration was higher in α-cellulose and pectin diet. HDL-cholesterol/total cholesterol ratio was higher in α-cellulose and pectin diet than alginic acid diet. In conclusion, alginic acid is good for the serum triglyceride decrease, pectin is good for HDL-cholesterol increase, α-cellulose and alginic acid are good for HDL-cholesterol/total cholesterol ratio increase. But the different effects according to the dietary fiber's contents did not show a significant trend.

원자력 사고 중 핵종의 건·습침적에 따른 농작물 오염 영향

황원태,김은한,서경석,한문희,최용호,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

핵종의 지표 침적 모델의 고찰과 함께 방사성물질의 공기중 농도로부터 건침적 뿐 아니라 습침적에 따른 농작물 오염 영향을 분석, 고찰하였다. 이를 위해 방사성물질의 지표 침적량으로부터 농작물의 오염을 평가하는 기존 동적 섭식경로모델을 공기중 농도 또는 지표 침적량으로부터 평가할 수 있도록 개선하였다. 평가결과, 방사성물질의 지표 침적량은 습침적에 의한 영향이 건침적에 의한 영향보다 뚜렷이 높으나, 농작물의 오염정도는 핵종, 강우율 등에 따라 다르게 나타났다. 이러한 현상은 방사성물질의 지표 침적과 농작물에로의 차단중 어느 과정이 농작물 오염에 보다 지배적으로 작용하는가에 기인한다. Combined with deposition model onto the ground of radionuclides, the influence of radioactive contamination to agricultural products was analyzed due to wet deposition as well as dry deposition from radioactive air concentration during a nuclear emergency. The previous dynamic food chain model, in which initial input parameter is only radionuclide concentrations on the ground, was improved for the evaluating of radioactive contamination to agricultural products from either radionuclide concentrations in air or radionuclide concentrations on the ground. As the results, in case of deposition onto the ground, wet deposition was more dominant process than dry deposition. While the contamination levels of agricultural products were dependent on the a variety of factors such as radionuclides and rainfall rate. It means that the contamination levels of agricultural products are determined from which is more dominant process between deposition on the ground and interception onto agricultural plants.

Allium 속 향신채 첨가가 찐어묵의 저장성과 품질에 미치는 영향

황지희,조은자 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.1

The effects of the addition of herbs belonging to Allium species on the quality and storage characteristics of kamaboko were studied, The herbs employed in the study were garlic(Allium sativum for, Pekinenese Makino), leek(Allium tuberosum Roth), onion(Allium cepa Linnaeus), and onion skin. water activity(Aw), pH, TBA, VBN, microbial load, textural characteristics and sensory evaluation were tested. 1. Aw of all the samples decreased on storage, the Aw on 10th day ranged from 0.937~0.950. All the samples containing herbs retained the pH 6~7 during the entire storage period even though it decreased gradually on storage. 2. In general, TBA and VBN of the samples containing herbs were lower than the control. The sample containing 3% onion skin showed the lowest TBA and VBN value. 3. The total plate count of the samples containing herbs was low compared to the control even though the total count increased during the storage. The samples containing 3% garlic and 3% onion skin showed the lowest total plate count on 20 days of storage. 4. The sample with garlic showed remarkably low value in sensory evaluation. The samples containing onion and onion skin, however, reached to the high sensory points as storage period increased. They received high points in taste as well as overall acceptance.

쌍발 복합재 비행기의 속도, 고도 보정 및 상승성능에 관한 연구

황명신,박윤진,이정모,김칠영,은희봉 한국항공운항학회 1997 한국항공운항학회지 Vol.5 No.1

Airspeed and altimeter calibration of "win Mee was conducted by the flight test. We have adopted system to system method. Flight test data is corrected for instrumented error and position error, and the resultin data was satisfied. Climb Performance flight test also was conducted. But we could not have all data because of limited flight time. The resulted data was satisfied compare with calculated dab.

원전 중대사고시 피폭경로 및 핵종의 방사선 피폭에 대한 상대적 중요도 해석

황원태,김은한,김병우,서경석,한문희 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3

원자력발전소의 중대 사고시 대기로 방출된 방사성물질에 의해 피폭자가 사고후 일생동안 받게 될 전신 피폭선량의 핵종의 상대적 중요도를 방출점으로부터 거리에 따라 각 피폭결로에 대해 평가하였다. 방사능운과 지표에 침적된 방사성물지에 의한 외부피폭,호흡과 오염된 음식물섭취에 의한 내부피폭이 피폭경로로 고려되었다. 오염된 음식물섭취에 의한 영향은 우리나라 환경을 고려하여 개발된 동적 섭식경로모델 KORFOOD를 사용하여 침적시점과 침점후 시간에 따른 음식물내 방사성물질의 농도 변화를 고려하였다. 방출점으로부터 80 km까지 피폭선량을 평가한 결과, 오염된 음식물섭취에 의한 영향이 가장 놓았다. 핵종별 기여도는 방사능운에 의한 외부피폭과 호흡에 의한 내부피폭의 경우 I, 침적된 방사성물질에 의한 외부피폭의 경우 Cs에 의한 영향이 가장 높았다. 오염된 음식물섭취에 의한 내부피폭의 경우 Cs은 여름철 침적, Sr은 겨울철 침적에 보다 중요한 영향을 미쳤다. In the case of a severe accident of a nuclear power plant, the whole body does and the relative importance of the radionuclides during the lifetime of an exposed person were estimated for each exposure pathway with distances from the release point. The external exposure pathways due to immersion of radioactive could and deposition of radioactive materials on the ground, and the internal exposure pathways due to inhalation and ingestion of contaminated foodstuffs were estimated considering the variation of radioactive concentration in the foodstuffs according to deposition time and elapsed time after deposition using a dynamic ingestion pathway model applicable to Korean environment, named "KORFOOD". As the results up to 80 km from the release point, the effects due to ingestion of contaminated foodstuffs showed the highest contribution to total exposure does. The contribution of I isotopes was the highest in the case of the external does due to immersion of radioactive cloud and internal does due to inhalation. The contribution of Cs isotopes was highest in the case of the external does due to deposition of radioactive materials on the ground. In the case of the internal does due to ingestion of contaminated foodstuffs, Cs deposition in summer and Sr deposition in winter, respectively, were the most dominant radionuclide to whole body.

공동주택 서포트의 공간가변성능 평가모델 작성연구

황은경,이강희,김수암 대한건축학회 2004 대한건축학회논문집 Vol.20 No.6

In recently, housing market has changed from new construction to building' stocks maintenance, and from centered on the supplier to users. It suggests that the occupant should change the space form to adapt the life stage at any time. For this, open building is very effective to satisfy the occupant's requirements. Open housing is proposed as a alternative design methods not only to compromise user's various needs but also to be a means of sustainable development by adapting long-life of buildings. Open building could be required to evaluate the capacity-to-change at design stage whether it adapts or copes with the occupant's needs. The evaluation of capacity-to-change is very important to grasp how much open building has the function or performance. The capacity-to-change of open building can be measured through model which is organized and explained the support's attributed. This paper aimed at providing the model to grasp the capacity-to-change of open building with support's components and attributes. For this, it selected the support's factor explaining the capacity-to-change of open building, and the effective number of sample is 81. Various factors would be integrated or controlled through multidimensional scaling. The evaluation model would be set up, utilizing the backward regression method.

폴리우레탄을 이용한 Thiobacillus sp. IW의 고정화

황은상,이광우,박돈희 전남대학교 촉매연구소 2001 觸媒硏究 論文集 Vol.22 No.-

The immobilization of Thiobacillus sp. IW, the hydrogen sulfide removing bacterium, was studied by using the adsorption and physical capture methods. In the case of immobilization by the adsorption, the type, size, and amount of polyurethane foam were affected by the cell immobilization capacity of supporter. Among supporters, MC-70 was better immobilizing material. The optimum size and amount of polyurethane foam were 5∼8mm and 8g/L, respectively. In the case of immobilization by the physical capture, polyurethane foam could be prepared with the same ratio of modified MDI prepolymer, polyol, and medium with better condition. The optimum size and amount of polyurethane foam were 6∼7mm and 8g/L, respectively. Considering the cell immobilization capacity, the immobilization by physical capture was more effective than the immobilization by adsorption. The immobilization intensity by adsorption was 85% after shaking for 24hrs, whereas the immobilization by physical capture was 92% under the same condit