키토산 구슬을 이용한 가토 연골세포의배양

장의찬,김경환,저호중,이기현,정상인,손태일 대한정형외과연구학회 2003 대한정형외과연구학회지 Vol.6 No.2

대장선암에서 p53 과 P-glycoprotein의 분포에 관한 연구

강상균,김의한,장인성,김대중,양승하 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Adenocarcinoma of colon and rectum express many oncogenes. The p53 acts as tumor suppressor in normal tissue but mutant forms are known as doing important role in carcinogenesis of many malignant tumors, such as colorectal cancer, gastric cancer, and breast cancer. P-glycoprotein shows distribution in many normal tissues and malignant tumor. This protein occurs multidrug resistance to therapeutic anticancer drug, resulting in poor prognosis of patients. The expression of p53 and P-glycoprotein has been studied by many authors according to clinicopthologic findings of colorectal adenocarcinoma, but these results are controversial yet. To observe the relationship between both antigen and pathologic findings, including prognostic factos, of colorectal adenocarcinoma, we analysed 13 cases adenoma and 62 cases adenocarcinoma then processed for immunohistochemical stain by SLAB method using mouse monoclonal anti-p53 and JSB-1. The results were as followings; 1) The expression rate of p53 in adenoma and adenocarcinoma are 38.5% and 71.0%, respectively, and P-glycoprotein are 46.6% and 58.1%, respectively. 2) The p53 revealed higher expression rate in case of well differnetiated form, invasion to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of moderately and poorly differentiated form, invasion to muscle, no lymphatic metastasis, but no difference accoding to site. 3) P-glycoprotein revealed higher positive rate in case of right side, invasion to surrounding adipose tissue, lymphatic metastasis compared to that of left side, invasion to muscle and serosa, no lymphatic metastasis, but no difference according to differentiation. 4) Both of p53 and P-glycoprotein revealed higher ex pression in case of right side, well and moderately differentiated form, invation to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of left side, poorly differentiated form, invasion to muscle, no lymphatic metastasis. Above results revealed that the expression of p53 begins in adenoma and shows close relation with development of colorectal carcinoma. p53 and P-glycoprotein may reveal the relation with poor prognosis. Adenocarcinoma showing both positive reaction of p53 and P-glycoprotein is also similar prognostic relation.

All-in-one 접착제에서 초음파진동이 법랑질과 상아질의 결합강도와 레진침투에 미치는 영향

이범의,장기택,이상훈,김종철,한세현 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.1

초기의 접착 시스템은 여러 단계의 술식을 필요로 하였으며 술자의 기술과 재료의 성질에 크게 좌우되었으나 최근 산부식, priming, adhesive를 한번에 적용할 수 있는 a11-in-one adhesive system이 등장하였다. 치과에서의 vibration의 이용은 치석의 제거 및 접착제의 점도를 낮추는데 이용되어왔으며 vibration은 접착제의 흐름성을 향상시켜 film thickness를 낮추어 수복물 주위의 미세누출을 줄이는데 도움을 준다. 이에 저자들은 all-in-one adhesive system에서 vibration이 법랑질과 상아질의 접착강도와 레진침투에 미치는 효과를 알아보고자 하였다. 법랑질 시편은 발거 후 실온에서 0.1% thymol 용액에서 보관된 30개의 건전한 사람의 대구치를 무작위로 10개씩 세군으로 나누고 근원심 방향으로 두 부분으로 분리하여 각각은 같은 접착제를 사용하고 초음파진동여부를 다르게 하였고, 아크릴 레진을 이용하여 직경 1-inch의 PVC관에 매몰한 후 협설면이 아크릴봉과 동일한 높이가 되도록 220-, 600-grit 연마지로 순차적으로 연마하였고 군당 10개씩 여섯 군으로 분류하였다. 1군과 2군은 Prompt L-Pop(3M-ESPE, Seefeld, Germany), 3군과 4군은 One-Up Bond F(Tokuyama Corp., Tokyo, Japan). 5군과 6군은 AQ bond(Sun Medical Co., Kyoto, Japan)를 제조사의 지시에 따라 도포하였다. 2군, 4군, 6군은 초음파 치석제거기를 이용하여 치면에 대고 15초간 진동을 가한 후 광중합하였다. 상아질 시편은 치관부 법랑질을 제거한 후 상아질면을 아크릴 봉과 동일한 높이가 되도록 하고 법랑질 시편과 동일하게 처리하였다. 이후 직경 2mm, 높이 3mm의 Teflon mold(Ultradent, U.S.A.)를 이용하여 복합레진을 충전한 후 40초씩 두 번에 나누어 광중합한 후 24시간동안 실온에서 증류수에 보관하였다. 열순환 시행한 후, 만능측정 기(Instron4465)로 전단결합강도를 측정하였으며 Resin tag의 양상을 비교하기 위해 각 군의 시편의 치질을 완전히 용해 시킨 후 표면을 주사전자현미경사진으로 관찰하여 다음과 같은 결과를 얻었다. 1. 법랑질에서 초음파 진동을 가한 군(2,4,6군)은 가하지 않은 군(1,3,5군)에 비해 평균 전단결합강도가 높게 나타났다. 그 차이는 AQ bond 군을 제외하고 통계적으로 유의하였다(p<0.05). 2. 상아질에서 초음파 진동을 가한 군(2,4,6군)은 가하지 않은 군(1,3,5군)에 비해 평균 전단결합강도가 높게 나타났다. 그러나 그 차이는 One-Up Bond F군을 제외하고는 통계적으로 유의한 차이가 없었다. 3. 전자 현미경 관찰에서 초음파 진동을 가한 군에서 더 많은 법랑질의 소실과 상아질에서 resin tag의 길이가 길었고 lateral branch의 수도 많이 관찰되었다. The objective of this study was to apply the vibration technique to reduce the viscosity of bonding adhesives and thereby compare the bond strength and resin penetration in enamel and dentin achieved with those gained using the conventional technique and vibration technique. For enamel specimens, thirty teeth were sectioned mesio-distally. Sectioned two parts were assigned to same adhesive system but different treatment(vibration vs non-vibration) Each specimen was embedded in 1-inch inner diameter PVC pipe with a acrylic resin. The buccal and lingual surfaces were placed so that the tooth and the embedding medium were at the same level. The samples were subsequently polished silicon carbide abrasive papers. Each adhesive system was applied according to its manufacture's instruction. Vibration groups were additionally nitrated for 15 seconds before curing. For dentin specimen, except removing the coronal part and placing occlusal surface at the mold level, the remaining procedures were same as enamel specimen. Resin composite(Z250 3M. U.S.A.) was condensed on to the prepared surface in two increments using a mold kit(Ultradent Inc., U.S.A.) Each increments was light cured for 40 seconds. After 24 hours in tap water at room temperature, the specimens were thermocycled for 1000cycles.. Shear bond strengths were measured with a universal testing machine(Instron 4465, England). To investigate infiltration patterns of adhesive materials, the surface of specimens was examined with scanning electron microscope. The results were as follows : 1. In enamel, the mean values of shear bond strengths in vibration groups(group 2,4,6) were greater than those of non-vibration group(group 1,3,5). The differences were statistically significant except AQ bond group. 2. In dentin, the mean values of shear bond strengths in vibration groups(group 2 4, 6) were greater than those of non-vibration groups(group 1,3,5). But the differences were not statistically significant except One-up Bond F group. 3. The vibration group showed more mineral loss in enamel and longer resin tag and greater number of lateral branches in dentin under SEM examination.

씨름 들배지기 기술의 키네메틱 특성분석

김의환,홍장표,우성돈,정재욱 龍仁大學校 武道硏究所 2001 武道硏究所誌 Vol.12 No.1

The purpose of this study was to analyze the kinematic variables Dul-baejiki, the most frequently used and crucial technique in Ssireum(Korean Traditional Westling) with 3dimensional analysis technique and show the kinematic characteristics of it. The subjects of this experiment were two best skilled players who have been plying in professional Ssireum team and whose weights are over 100kg(subject A) and under 100kg(subject B) each. Kinematic variables analyzed here include time, posture, and center of gravity. The mean values and the standard deviations for each variables were variables were obtained and used as basic factors for examing characteristics of Dul-baejiki motion From the data analysis discussion, the following conclusions were drawn: 1) Temporal variables The average time elapsed(TE) by phases of Dul-baejuki motion are the following. For subject A(over 100kg), TE during 1st phase was 0.69 sec(53.5%), TE during 2nd phase was 0.44 sec(34.1%), TE during 3rd phase was 0.16 sec(12.4%). For subject B(under 100kg), TE during 1st phase was 0.69 sec(55.6%). TE during 2nd phase was 0.36 sec(29.1%), TE during 3rd phase was 0.19 sec(15.3%). In the TE by phases of both subjects, the longest TE was the 1st phase, next the 2nd phase, and then the 3rd phase. 2) Center of Gravity(COG) variables Frontal Plane for both subjects showed a continuous movement rightward from pulling phase to falling phase. For Sagital Plane, subject Q moved backward in pulling phase, but subject B moved 6 cm forward until falling phase and then moved backward. For Height, subject A was situated 87.3 cm ∼106.7 cm high from the mat from initial phase throughout falling phase, subject B was situated 75 cm ∼97.1 cm.

정중과잉치로 인해 회전된 상악 중절치의 치험례

이범의,김영재,김정욱,장기택,이상훈,김종철,한세현 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

정중과잉치의 원인은 잘 알려지지는 않았지만 dental lamina의 hyperactivity에 의해 발생한다는 가설이 가장 많이 받아들여지고 있다. 이러한 정중과잉치의 병발증으로는 주변 영구절치의 맹출 장애, 만곡 치근, 치근흡수, primordial 혹은 follicular cyst의 형성, 회전, 이환된 부위의 총생 및 비정상적인 정중이개를 일으킬 수 있다. 이러한 이유로 영구전치의 회전이 야기되면 보통 공간의 부족은 적거나 없으며 재발이 흔하므로 과개선이 요구된다. 재발의 방지를 위해서는 조기치료, 과개선, 긴 보정기간, 적절한 접촉면의 형성, 짝힘의 사용, 그리고 수술적인 방법 등이 제안되어왔다. 이에 저자는 정중과잉치로 인하여 심하게 회전된 상악중절치를 주소로 내원한 환아에서 교정적, 외과적 술식을 이용하여 양호한 결과를 얻은 두 증례를 보고하는 바이다. The etiology of mesiodens is unknown but the most widely accepted theory is the hyperactivity of the dental lamina. Complications of mesiodens are delayed or prevented eruption of maxillary central incisors, displacement or rotation of permanent teeth, crowding of affected region, abnormal diastema or permanent space closure, dilaceration or abnormal root development of permanent teeth, primordial or follicular cyst formation, root resorption of adjacent teeth, eruption into nasal cavity. If mesiodens rotate the maxillary central incisors, space deficiency is not common and relapse is very common. So overcorrection is needed. To prevert the rotational relapse, early treatment, overcorrection, long retention period, properly formed proximal surface, use of coupled force, and surgical techniques have been suggested. The authors present two cases, whose chief complain were severely rotated maxillary incisors by mesiodens, treated by orthodontic and surgical technique and showed good results.

질산화 작용이 있는 Aeromonas hydrophila의 동정 및 특성

엄미나,장재철,유영희,지의상 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.6

폐수처리 중 생물학적 처리에 활용할 수 있는 질소분해 능력을 가진 미생물을 분리하여 동정하고자 경기도내 하천 6지점에서 채취한 시료로부터 50개 균주를 선별하였다. 형태학적, 생화학적 및 배양학적 실험결과 Bergey's mannual of systematic bacteriology의 색인을 통하여 Aeromonas hydrophila로 동정하였다. Aeromonas hydrophila(AH-1). (AH-3), (AH-4), (AH-6) 균이 질산화 능력이 우수하였다. 4개 균주 모두 amoxillin, ampicillin, cephalothin과 ticarcillin에 내성을 나타내었다. 본 실험에서 분리한 .Aeromonas hydrophila의 질산화의 최적조건은 균 농도 1.0×l0exp(6) cells/ml, 배양온도 37℃로 나타났다. For the purpose of the isolation of microorganisms which have the capability of nitrification, we isolated the microorganisms in 6 samples collected from the stream of Kyonggi area. 60 strains were isolated. The selected strain were identified as a Aeromonas hydrophila based on the data obtained from the morphological, biochemical and cultural characteristics defined experiments. Among them Aeromonas hydrophila (AH-1), (AH-3), (AH-4), (AH-6) showed the highest nitrification capability. All isolates were resistant to amoxillin, ampicillin, cephalothin and ticarcillin. Optimum culture conditions of isolates were 37℃ and 1×10exp(8) cells/ml for 4 hours in the nitrate medium.

성인의 만성 기침에 대한 감별진단으로서의 백일해

박완범,박상원,이기덕,이창섭,장희창,김홍빈,김의종,오명돈,최강원 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 백일해는 소아기에 발생하는 전염병으로 오랫동안 알려져 왔다. 그러나, 미국과 유럽에서는 1990년대에 들면서 성인에서도 백일해가 유행하며, 만성 기침 환자의 20% 정도가 백일해를 앓는 것으로 보고하고 있다. 저자들은 국내에서 성인 만성기침의 원인으로 백일해의가능성을 확인하고자 하였다. 재료 및 방법 : 2002년 9월부터 2003년 5월까지 서울의 일개 대학보건진료소 또는 일개 시립병원 외래를 방문한 환자 중 기저 폐질환 없이 1주 이상 기침을 하는 성인을 대상으로 Bordetetlla pertussis에 대한 배양검사와 중합효소 연쇄반응검사(PCR)를 시행하였다. 결과 : 1주 이상의 기침을 하는 성인 102명 중 배양검사에서 양성인 환자는 없었으나 3명(2.7%)에서 PCR 양성이었으며 이들 모두 3주 이상 기침이 지속되었고 백일해의 특징적인 증상을 호소하였다. 결론 : 국내에도 성인에서 백일해가 발생하고 있음을 확인하였고 따라서 성인 만성기침의 원인으로 백일해를 감별 진단해야 한다. Background : Pertussis was long considered a childhood illness. However, in the last two decades, it has been reported as a cause of prolonged cough in adolescents and adults in other countries. Infection of Bordetella pertussis was prospectively searched among adults with a persistent cough. Materials and Methods : Adult patients, who visited either the outpatient clinic of a municipal hospital or a university health service center due to cough of more than six days' duration without underlying pulmonary disease, from September 2002 to May 2003, were enrolled. The culture and polymerase chain reaction (PCR) were used for confirming of B. pertussis infection. Results : 102 adult patients with persistent cough were evaluated. 3 (2.9%) patients were PCR positive for B. pertussis. There were no patients with positive culture. All patients with positive PCR had one or more classic symptoms of pertussis and their cough persisted for 3-7 weeks. Conclusion : We confirmed the morbidity of pertussis in Korean adults with persistent cough. Pertussis should be considered as a differential diagnosis of chronic cough in adults.

공터키안(Empty sella)을 동반한 급성췌장염으로 발현된 부갑상선 선종 1예

전언주,오지혜,배경륜,장샛별,전승운,정의달,손호상,원규장 영남대학교 의과대학 2009 Yeungnam University Journal of Medicine Vol.26 No.1

The incidence of coexisting hyperparathyroidism and empty sella syndrome is rare and the etiology and incidence of their coexistence is not known. The association of hyperparathyroidism and the empty sella syndrome may be related to multiple endocrine neoplasia (MEN) syndrome due to a genetic disorder. We experienced a rare case of hyperparathyroidism presenting as acute pancreatitis combined with empty sella. We report here a 37-year old female who manifested epigastric pain because of acute pancreatitis. She had hypercalcemia due to parathyroid adenoma. A pituitary gland was not visible in the sella turcica on MRI scans. On genetic analysis, she did not show a mutation of the MENIN gene. Empty sella is thought to be a coincidental finding with hyperparathyroidism.

Antibiotic Resistance and Genetic Diversity of Listeria monocytogenes Isolated from Chicken Carcasses in Korea

( Sung Sik Jang ),( Eui Young Choo ),( Ki Seon Han ),( Takahisa Miyamoto ),( Sung Gi Heu ),( Sang Ryeol Ryu ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.8

Very Efficient Nucleophilic Aromatic Fluorination Reaction in Molten Salts: A Mechanistic Study

Jang, Sung-Woo,Park, Sung-Woo,Lee, Byoung-Se,Chi, Dae-Yoon,Song, Choong-Eui,Lee, Sung-Yul Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.3

We report a quantum chemical study of an extremely efficient nucleophilic aromatic fluorination in molten salts. We describe that the mechanism involves solvent anion interacting with the ion pair nucleophile $M^+F^-$(M = Na, K, Rb, Cs) to accelerate the reaction. We show that our proposed mechanism may well explain the excellent efficiency of molten salts for SNAr reactions, the relative efficacy of the metal cations, and also the observed large difference in rate constants in two molten salts $(n-C_4H_9)_4N^+\;CX_3SO_3^-$, (X=H, F) with slightly different sidechain ($-CH_3$ vs. $-CF_3$).