Nitrogen Catabolite Repression of Asparaginase II in Saccharomyces cerevisiae: I. Secretion and Purification of Asparaginase II from Yeast Spheroplasts

정유진,김규원,Jeong, Yoo-Jin,Kim, Kyu-Won Korean Society for Biochemistry and Molecular Biol 1990 한국생화학회지 Vol.23 No.3

효모의 질소대사에 관여하는 asparaginase II의 분비와 Nitrogen Catabolite Repression(NCR)의 조절작용을 효모 스페로플라스트를 이용하여 조사하였다. 즉 효모 스페로플라스트에서도 배지에 질소원이 부족하면 asparaginase II 생성의 억제가 제거되고, 그 억제의 제거에 필요한 시간과 효소의 생성량도 정상 효모 세포와 유사한 양상을 나타내었다. 또한 스페로플라스트에 의한 효모의 생성이 정상 세포와 동일하게 NCR의 조절기작에 의해 이루어짐이 밝혀졌다. 아울러 이 시스템을 이용하여 asparaginase II를 용이하게 정제할 수 있었으며, SDS-PAGE에서 정상세포와 동일한 두 개의 단백질 밴드를 얻었다. We have studied the synthesis and secretion of the exoenzyme asparaginase II by yeast spheroplasts. When spheroplasts are starved for nitrogen they derepress the synthesis of asparaginase II. The time course of the derepression and the level of enzyme synthesis were comparable for spheroplast and whole cell cultures. Furthermore, spheroplast enzyme synthesis was as sensitive to nitrogen catabolite repression as was synthesis by whole cells. We have used the purification procedure in the spheroplast to show that two protein components resolved by SDS gel electrophoresis are associated with asparaginase II.

실시간 수질모니터링시스템에 대한 제언

정유진,Jeong, Yu-Jin 한국수자원학회 2010 물과 미래(한국수자원학회지) Vol.43 No.4

식용색소에 의한 CEROMER 수복물의 색안정성

정유진,임주환,조인호,임헌송,Jeong Yu-Jin,Lim Ju-Hwan,Cho In-Ho,Lim Heon-Song 대한치과보철학회 2003 대한치과보철학회지 Vol.41 No.2

Statement of problem : Ceramic and composite resin have been used to fulfill the demand for esthetic prosthesis. However, ceramic is easy to break and wears off the opposite natural teeth. Conventional composite resin also has low abrasive resistance and color stability. Ceramic Optimized Polymer (ceromer) was developed in mid-1990s to overcome the shortfalls of ceramic and composite resin. Ceromer has similar abrasiveness with the natural tooth and has relatively high strength. Color stability affects esthetics and long-term prognosis of the prosthesis. Purpose The purpose of this study was to compare color stability of ceromers(2 types : $Artglass^{(R)}$. $Targis^{(R)}$) with ceramics ($Vintage^{(R)}$-polishing, $Vintage^{(R)}$-glazing). Material and Method : The color difference(${\Delta}E^*$) was measured by spectrophotometer with different immersion time. Twenty disks, 3mm in thickness and 10mm in diameter, were fabricated for each specimen in shade A2(Vita Lumin shade guide), Specimens (5 samples in each group) were immersed in the food colorants (Red no.3. Yellow no.4, Blue no.1, Distilled water) for 24 hours, 48 hours and 72 hours respectively. $L^*,\;a^*$ and $b^*$ value were measured with spectrophotometer (CM 503i : Minolta Co., Japan) and mean ${\Delta}E^*$ value was calculated for statistical analysis Results : The results of this study were obtained as follows. 1. The ${\Delta}E^*$ values of all test samples increased with the time of immersion. 2. The ${\Delta}E^*$ values of all materials increased in order of Distilled Water, Yellow no.4, Blue no.1 and fed no.3. There was significant difference between Red no.3 and the other food colorants(p<0.05). 3. The ${\Delta}E^*$ values increased in order of $Vintage^{(R)}$-glazing, $Vintage^{(R)}$-polishing. $Artglass^{(R)}$ and $Targis^{(R)}$. There was significant difference between $Vintage^{(R)}$-glazing and the other materials (p<0.05). Conclusion : By means of the above results, immersion time was found to be a critical factor for color stability of ceromer. For the long-term color stability of prosthesis it is recommended patients having ceromer prosthesis ($Artglass^{(R)},\;Targis^{(R)}$) to reduce the habitual intake of Red no.3 colorants con taming foods.

수중 환경에서 효율적인 무선 통신을 위한 매체접근제어 메커니즘

정유진,신수영,박수현,Jeong, Yoo-Jin,Shin, Soo-Young,Park, Soo-Hyun 한국시뮬레이션학회 2006 한국시뮬레이션학회 논문지 Vol.15 No.4

수중과 같은 에러의 변동이 크고 무선 통신에 제약이 따르는 환경에서 데이터 전송을 실행하게 되는 경우에는 상당히 심각한 지연율과 에러 발생률을 고려하지 않을 수 없다. 따라서 이러한 환경에서 사용되는 메커니즘은 가능한 한 효율적이면서 간단해야만 한다. 본 논문에서 제안하는 Pervasive Block ACK(PBA) 기법은 각 프레임마다 전송되던 ACK를 Set 단위로 묶어서 한꺼번에 보내주는 방법으로, 수중 등 열악한 환경에서 ACK 전송으로 인해 발생하는 트래픽 발생빈도 감소를 위해 제안되었다. Pervasive Block ACK 기법은 전송 횟수를 최소로 줄임으로써 오버헤드를 감소시켜 네트워크의 효율성을 높여 지연율은 줄일 뿐만 아니라 그에 부가적으로 전력 소비 절감의 효과까지도 기대할 수 있다. 본 논문에서는 Numerical Result를 통하여 제안에 관한 효율성 및 연구 가치를 증명하였다. Data transmission in poor environment like underwater has considerably serious delay rate and ewer rate. Like this environment usually has heavy fluctuation of error rate and limited wireless communication state. Therefore, mechanism using in such environment has to be efficient and simple. This paper suggests a new block ack mechanism, called the Pervasive Block ACK (PBA), which transmits aggregated ACKs. This mechanism takes effect on reducing number of traffic, decreasing overhead and delay rate in poor environment networks like underwater. Additionally, we can expect energy consumption. We verify propriety and efficiency of PBA through describing numerical result based analytical formula in this paper.

통합 의학 치료로 5년 생존 및 완전 관해에 도달한 췌장암 증례 보고 1례

정유진,김지수,박경덕,오유나,정범진,방선휘,Yu-jin Jung,Jisoo Kim,Kyung-Dug Park,Yoona Oh,Beom-Jin Jeong,Sunhwi Bang 대한한방내과학회 2023 大韓韓方內科學會誌 Vol.44 No.3

Objectives: This is a five-year survival and complete response (CR) report on pancreatic cancer treated with western medicine and Korean traditional medicine. Method: A 59-year-old woman diagnosed with pancreatic cancer visited ○○ Korean traditional medicine hospital after neoadjuvant chemotherapy and pylorus-preserving pancreaticoduodenectomy. She was treated with Korean traditional medicine, including acupuncture, abdominal moxibustion, wild ginseng pharmacopuncture, and herbal medicine, which was based on integrated medicine therapy (IMT), from March 2018 to September 2022. The tumor size was measured by scanning with computed tomography (CT), magnetic resonance imaging, and positron-emission tomography/CT. Adverse events were evaluated using laboratory conclusion and National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0. Result: During four years and three months of treatment, IMT maintained safety. The patient finally reached five-year survival without any recurrence or complication (CR) on October 26, 2022. Conclusion: We suggest that an integrative approach including Korean traditional medicine can be a meaningful treatment option for pancreatic cancer. Further studies should be performed to establish the proper treatment protocol of integrative medicine for pancreatic cancer.

효모 ( S . cerevisiae ) asparaginase Ⅱ 의 Nitrogen catabolite repression 에 관한 연구 : Ⅰ. 효모 스페로플라스트로부터 asparaginase Ⅱ의 분비와 정제

정유진,김규원 ( Yoo Jin Jeong,Kyu Won Kim ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.3

We have studied the synthesis and secretion of the exoenzyme asparaginase II by yeast spheroplasts. When spheroplasts are starved for nitrogen they derepress the synthesis of asparaginase II. The time course of the derepression and the level of enzyme synthesis were comparable for spheroplast and whole cell cultures. Furthermore, spheroplast enzyme synthesis was as sensitive to nitrogen catabolite repression as was synthesis by whole cells. We have used the purification procedure in the spheroplast to show that two protein components resolved by SDS gel electrophoresis are associated with asparaginase II.

소아 종양 환아의 중추 신경계 합병증

정유진,서연경,홍승아,김흥식,김준식,이희정,Jeong, Yoo Jin,Seo, Yeon Kyong,Hong, Seung-A,Kim, Heung Sik,Kim, Jun Sik,Lee, Hee Jung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.11

목 적 : 소아의 악성 종양에 대한 새로운 치료법의 개발로 환아의 생존 기간이 증가하는 추세에 있으나 이로 인한 신경계 합병증의 발생도 증가하고 있다. 저자들은 항암 치료를 시행한 소아 종양 환아에서 나타난 중추 신경계 합병증에 대한 임상 양상을 알아보고자 하였다. 방 법 : 1995년 1월부터 2002년 11월까지 동산병원 소아과에서 항암치료를 시행한 환아 중 신경계 합병증이 동반되었던 15례의 환아를 대상으로 그 임상상을 후향적으로 검토하였다. 결 과 : 전체 15례 중에서 방사선학적 검사로 원인 질환을 밝힌 경우는 모두 13례로 백질뇌병증이 7례, 무기질화 미세혈관병증이 4례, 뇌경색이 3례, 뇌출혈이 1례, 저산소성 허혈성 뇌증이 1례, 2가지 이상의 합병증을 함께 나타낸 경우는 2례였으며, 혈액학적 방사선학적 검사상에서 이상 소견이 없이 MTX의 부작용으로 생각되는 경련성 질환이 2례였다. 뇌출혈과 뇌경색이 나타낸 경우는 예후가 나빴으며 전해질 불균형으로 인한 저산소성 허혈성 뇌증에서는 원인 교정 후 후유증 없이 회복되었다. 결 론 : 소아 종양 환아에서 치료 중 발생한 신경계 합병증은 질환의 예후에 큰 영향을 미치고 신경인지 발달에 장애를 남기므로 이들 합병증에 대한 예방, 진단 및 즉각적인 치료와 추적관찰이 필요할 것이다. Purpose : Recent advances in the methods of treating cancer in young patients have led to both an increased frequency of CNS complications as well as prolonged life expectancy. We intend to analyze the clinical aspects and laboratory findings of patients with CNS complications during and after treatment. Methods : We reviewed the medical records of 174 childhood cancer patients treated with chemotherapy admitted to the Dept. of Pediatrics, Keimyung University Dongsan Hospital, from January 1995 to November 2002. Among them, 15 cases with CNS complications were investigated in this study. Results : CNS abnormalities were found in 13 patients by CT or MRI during treatment such as leukoencephalopathy(n=7), mineralizing microangiopathy(n=4), brain infarction(n=3), intracranial hemorrhage(n=1), and hypoxic ischemic encephalopathy(n=1). It was found that two patients had two or more CNS abnormalities. Two patients who had no imaging abnormalities had convulsions, possibly after the addition of intrathecal methotrexate. The patients with intracranial hemorrhage and brain infarction had rapid and fatal clinical courses. The hypoxic ischemic encephalopathy following electrolyte imbalance completely recovered after correction of electrolyte. Conclusion : The CNS complications that occur during and after chemotherapy influence prognoses significantly, and remain neurologic sequelae. Therefore early diagnosis and prophylaxis for CNS complications and regular physical examination of patients who have recieved cancer therapy are strongly recommended.

병원균 및 생물독소 탐지시스템을 위한 원자외선 LED 기술동향

정유진,정영수,최기봉,Chong, Eugene,Jeong, Young-Su,Choi, Kibong 한국전기전자재료학회 2015 전기전자재료학회논문지 Vol.28 No.5

The humans are under attack involving the hazardous environment and pathogen/biotoxin aerosol that is realistic concerned. A portable, fast, reliable, and cheap Pathogen and Biotoxin Aerosol threat Detection(PBAD) trigger is an important technology for detect-to-protect and detect-to-treat system because the man-made biological terror is a fast and lethal infection. The ultraviolet C(UVC) wavelengths light source is key issue for PBAD that is sensitive because of strong fluorescence cross section from fluorescent amino acids in proteins such as tryptophan and tyrosine. The UVC-light emitting diode(LED) is emerging light source technology as alternative to laser or lamps as they offer several advantages. This paper discussed about the design consideration of UVC-LED for the PBAD system. The UVC-LED and PBAD technology, currently available or in development, are also discussed.

신경모세포종 환아에서 레티노익산 치료 중 발생한 급성 췌장염 1례

정유진,서연경,김흥식,이희정,Jeong, Yoo Jin,Seo, Yeon Kyong,Kim, Heung Sik,Lee, Hee Jung 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.11

신경모세포종 환아에서 레티노익산 치료 중 급성 췌장염이 합병되어 사망한 1례를 경험하여 보고하는 바이다. 레티노익산을 사용하는 환아에서 췌장염을 예방하기 위해서는 지속적인 혈중 지방 수치 추적이 필요할 것으로 생각되고 치료 중 호전을 보이더라도 지속적인 감시와 관찰이 필요할 것으로 생각된다. Retinoic acid has been used successfully as a differentiating agent in acute promyelocytic leukemia and neuroblastoma. However, some adverse effects have been recognized, such as headaches, dry skin and retinoic acid syndrome, a life threatening acute cardiorespiratory disorder. Acute pancreatitis with hyperlipidemia has rarely been reported. We experienced a case of acute pancreatitis with hyperlipidemia in a neuroblastoma patient after retinoic acid therapy for 21 months. Although the patient was ordered nothing by mouth and total parenteral nutrition was administrated, she died of disseminated intravascular coagulopathy and pulmonary hemorrhage, possibly because of oral intake during her recovery period.

MAC 계층 소프트웨어의 구현 환경을 제공하기 위한 SystemC 기반의 가상 MCU 모듈의 설계 및 구현

정유진 ( Jeong Yoo Jin ),박수진 ( Park Soo Jin ),이호응 ( Lee Ho Eung ),박현주 ( Park Hyun Ju ) 한국인터넷정보학회 2009 인터넷정보학회논문지 Vol.10 No.6

무선통신 프로토콜의 구현에서 MAC 계층은 하드웨어와 소프트웨어를 결합한 시스템-온-칩으로 출시하는 것이 일반적이다. 하지만 이러한 시스템 개발에서 하드웨어의 개발에 많은 시간이 소요되므로 하드웨어의 개발 완료 이전에 소프트웨어의 개발 및 검증하기 위한 환경이 필요하다. 하드웨어와 소프트웨어의 통합 개발에서 하드웨어는 HDL(Hardware Description Level)을 이용한 RTL(Register Transfer Level) 로의 하드웨어 모델링을 통해서, 소프트웨어는 ISS를 통해 시뮬레이션 환경을 제공할 수 있다. 시스템의 개발 복잡도가 점차 증가함에 따라 기존 RTL(Register Transfer Level) 보다 높은 추상 레벨에서의 모델링을 이용하는 ESL(Electronic System Level) 설계가 이루어지고 있다. ESL 설계는 비시간 모델과 시간 모델로 나눌 수 있다. 본 논문에서는 시간 모델이 아닌 비시간 모델 시뮬레이션을 위한 MCU를 설계 및 구현한다. 제안하는 MCU는 비시간 모델에서 정확한 시간이 요구되는 부분 보다는 시스템의 동작을 쉽고 빠르게 검증함으로써 시스템 설계 초기 단계에 시스템의 최적화뿐만 아니라 설계 완료 시점을 앞당길 수 있다. 또한 운영체제를 구동할 수 있는 MCU 모듈을 설계함으로써 MAC 계층의 소프트웨어 부분을 실시간 운영체제 상에서 구현할 수 있는 환경을 제공할 수 있다. 따라서 본 논문에서는 SystemC 기반의 MCU 모듈과 실시간 운영체제 동작을 지원하는 UC/OS-II 모듈을 제안한다. The development of wireless communication MAC layer is usually released as SoC which is a combination in hardware and software. In this system development environment, an environment for software development and verification is necessary because the hardware development takes a lot of time priori to completion. In integrated development of hardware and software, simulation environment of hardware and software provided by hardware modeling using HDL at RTL and ISS respectively. By increasing the development complexity of system, ESL design modeling systems at higher abstraction level than RTL has already prompted. The ESL design is divided untime model and time model. This paper present design and implementation of MCU for untime model simulation, not time model. Proposed MCU can optimize the system at early step of system development and move up the development completion time by verifying the system function easily and rapidly than part required exact time in untime model. In this paper, we present an MCU module based on SystemC and UC/OS-II Module providing real-time operate system.