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박수현,정진서,최양도,김주곤 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.6
Here, we report characterization of an RbcS promoter and its transit peptide for use in chloroplast-targeted expression. RbcS3 that encodes a small subunit of ribulose bisphosphate carboxylase/oxygenase is of great interest due to its green tissue-specific expression such as leaf and leaf sheaths. We isolated the RbcS3 promoter and its transit peptide sequence and linked to GFP for characterization in transgenic plants. Our qRT-PCR analysis on leaves and roots of transgenic plants revealed that GFP transcript levels were higher by fivefold in leaves of RbcS3:TP3:GFP transgenic lines than in those of the RbcS3:GFP transgenic lines, suggesting that the transcript levels were increased by chloroplast-targeted expression, as shown in previously characterized RbcS1:TP1 system. The transit peptide sequence TP3 was sufficient to target GFP to chloroplasts as observed in protoplasts of RbcS3:TP3:GFP transgenic lines. GFP fluorescence in RbcS3:GFP plants was detected only in the cytosol. The GFP protein levels in leaves of RbcS3:TP3:GFP plants were 0.12–0.23 % of the total soluble leaf proteins. These levels were higher or comparable to those of PGD1:GFP plants (GFP driven by a constitutive promoter PGD1). Taken together, these results indicate that the fusion of the RbcS3 promoter to its transit peptide, RbcS3:TP3, provides a chloroplast-targeting expression system.
일제말 파시즘기(1937~1945) 『매일신보』의 대중선동 양상과 논리 -지원병ㆍ징병제도를 중심으로-
박수현 한국민족운동사학회 2011 한국민족운동사연구 Vol.0 No.69
The Japanese imperialism introduced the conscription system in Korea after the Sino-Japanese war. The enforcement of conscription was not that they trusted the Korean but that they had no choice for the reinforcement of lack troops. Therefore they did everything for the establishment of the conscription as quickly as possible. Above all, it was necessary for the Korean to be the subjects of Japanese empire. It was risk bearing to supply the Korean infused with the ideas of subjects unilaterally with weapon by force. That was why they put the most emphasis on the mass instigation pushing forward the conscription system. There were many ways of mass instigation, but Mail Shinbo was the key measure. It was the Japanese Government-General of Korea’s organ which reported the content, method and logic of mass instigation. Though the subscription was not high at that time, the content and logic of Maeil Shinbo were spread in the whole country by various means and media. Instigation by Maeil Shinbo was to create the festive atmosphere at the day of conscription promulgation or enforcement date by focusing on glorifying and exaggerating the Koreans’ response. And instigation logic were ‘the Korean were greatly benefitted(dispensation theory)’, ‘We should return the Japanese emperor’s favor(gratitude theory)’, and ‘Korea and Japan were one nation(theory of integration between Japan and Korea)’. As the war protracted, mass instigation had gradually lost its effects. However mass instigation was so influential in daily life and the idea of the Korean that some people voluntarily participated in the war mobilization. 일제 말 파시즘체제는 다양한 양상으로 운용되었으며, 대중사회 통제 또한 다양한 방법으로 이루어졌다. 강제적 특성만으로 일제 말 파시즘체제의 실체를 이해하는데 한계가 있을 수밖에 없고, 식민지 대중들의 다양한 삶의 모습을 이해하는데도 많은 한계가 있다. 이 글은 파시즘체제의 또 하나의 특성인 선동정치에 주목해, 일제가 지원병제도와 징병제도를 추진하면서 어떠한 양상으로 조선인들을 선동했고 그 논리는 무엇인가를 매일신보를 통해 파악하는데 중점을 두었다. 이에 덧붙여 식민지 대중들의 반응을 살폈다. 일제는 불안한 가운데 조선에 지원병ㆍ징병제도를 실시했다. 조선인을 신뢰할 수 없을 뿐더러 일본어 습득이나 사상 등 병역을 담당할 수 있는 조건, 즉 황민화도 미흡했기 때문이다. 그러나 조선인 병역문제는 조선통치의 근간을 흔드는 중대 사안이었고 또한 전황이 급박했기 때문에 일제는 어떻게든 제도를 정착시켜 황민화를 진전시키는 계기로 삼고자 했다. 그러나 황민화는 강압적 방법으로 이루어 질 수 없으며, 더욱이 불신이 가시지 않는 조선인을 강제로 군대로 내모는 것은 위험부담이 컸다. 일제가 선동정치에 총력을 기울인 이유가 여기에 있었다. 대중선동의 중요성과 필요성은 징병제도가 예상보다 크게 앞당겨지면서 더욱 커졌다. 전쟁동원을 위한 선동은 모든 수단 방법을 동원해 전방위적으로 이루어졌다. 『매일신보』는 조선총독부 기관지이자 한글신문으로서 대중선동의 중추적인 역할을 담당하며 여론을 몰아갔다. 『매일신보』가 대중들을 선동하기 위해 가장 중점적으로 다룬 기사는 병역제도의 선전ㆍ홍보와 조선인들의 동향과 관련된 내용이었으며, 이 내용들을 대부분 시혜론ㆍ보은론ㆍ내선일체론 등으로 포장해 조선인들의 전쟁협력과 참여를 독려했다. 이러한 『매일신보』의 선동 내용과 논리는 다시 다양한 수단과 매체를 통해 전국 구석구석까지 확산되었다. 대중선동이 일제 말 조선사회와 대다수 조선인의 삶 전체를 규정할 정도의 영향력은 아니었지만, 일상사가 되어버린 끊임없는 선동에 많은 조선인들이 영향을 받았고 그 중에는 선동에 휩쓸려 적극적으로 일제의 침략전쟁에 동참하는 조선인들도 적지 않았다. 대표적인 예가 지원병 지원 현상이다. 그러나 이러한 광란의 선동정치도 전쟁이 장기화 되면서 점차 본질이 드러나게 되었고, 영향력도 한계에 부딪치게 되었다.
호텔 직원의 직업소명의식과 조직기반 자긍심, 경력몰입 및 직무헌신행동간의 구조적 관계
박수현,이형룡 (사)한국관광레저학회 2019 관광레저연구 Vol.31 No.2
The purpose of this study is to analyze the structural relationships among the factors of job calling, OBSE, career commitment and job devotion behavior. A total of 371 employees working in ten five-star hotels in Seoul and Incheon were surveyed, and the results were empirically analyzed. The data were analyzed using frequence, reliability, confirmatory factor analysis and structural equation modeling with SPSS statistical program and AMOS 18.0. The results can be summarized as follows. First, hotel employees’ job calling caused a positive influence on OBSE, career commitment and job devotion behavior. Second, hotel employees’ OBSE no significant positive influence on career commitment. Third, hotel employees’ OBSE led to a positive influence on job devotion behavior. Fourth, hotel employees’ career commitment led to a positive influence on job devotion behavior. Furthermore, OBSE played a mediating role in the relationship among hotel employees’ job calling and job devotion behavior.
박수현,김예림,김여진,배종석,이주헌,김우경,송홍기 대한신경과학회 2023 대한신경과학회지 Vol.41 No.3
Neurocritical patients who can self-report pain use the 0-10 numerical rating scale (NRS, verbal or visual form). However, critically ill patients whose nervous systems cannot express pain use the behavioral pain scale (BPS) and the critical care pain observation tool (CPOT) behavioral pain assessment tools. These tools reveal pain-related changes in movement, facial expression, posture, and physiological indicators such as heart rate, blood pressure, and respiratory rate. In pain control, it is first essential to reduce unnecessary painkillers through non-drug therapy and maximize the effect of the administered analgesics. For nonneuropathic pain, narcotic analgesics such as fentanyl, hydromorphone, morphine, and remifentanil are administered intravenously. Gabapentin, pregabalin, and carbamazepine are recommended along with narcotic analgesics for neuropathic pain control. In addition, nonnarcotic analgesics for multi-modal analgesia are used to reduce the use of narcotic analgesics or the side effects of narcotic analgesics. In the intensive care unit (ICU), the sedationagitation scale (SAS) and the Richmond agitation-sedation scale (RASS) are used to determine the depth of sedation to be maintained during shallow or deep sedation, considering the condition of the critically ill patient. When selecting sedatives for critically ill patients, preferentially consider nonbenzodiazepines such as propofol or dexmedetomidine rather than benzodiazepines such as midazolam or lorazepam. In addition, patients use painkillers or sedatives for over a week, and neurological changes or physiological dependence may occur. Therefore, clinicians should evaluate the critically ill patient’s condition, and sedatives and painkillers should be reduced or discontinued.
박수현,김현기,조맹효,Park Soo-Hyun,Kim Hyun-Gi,Cho Maeng-Hyo 한국전산구조공학회 2006 한국전산구조공학회논문집 Vol.19 No.2
위상 최적화 문제는 다양한 밀도 분포를 가지는 설계영역에서 목적함수와 요소단위의 설계 민감도의 반복적인 계산을 요구한다. 최근 제안된 2단계 축소기법은 축소 시스템을 구축하는데 매우 효과적이며 고유치 문제와 동적 문제의 해석에 정확도와 효율성을 동시에 제공한다. 본 논문에서는 구조 위상 최적화 문제에서 해석 부분과 민감도 계산 부분에 2단계 동적 축소기법을 사용한다. 축소시스템에 대한 위상 최적화 결과는 축소되지 않은 전체 시스템에 대한 최적화 결과와 비교하여도 공학적으로 요구되는 정확도 범위 내에서 2단계 축소기법이 높은 정확도와 계산 효율을 보장하는 것을 보여준다. Topology optimization problem requires numerous repeated evaluations of objective function and design sensitivity for elements within design domain with various density distributions. The recently proposed two-level condensation scheme(TLCS) is very promising for the construction of reduced system and for an accurate and efficient analysis concerned about eigenvalue and dynamic problems. We used the two-level dynamic condensation scheme for the analysis and sensitivity computation part in the structural topology optimization problem. The results of the topology optimization for the reduced system show the TLCS provides high accuracy and computation efficiency compared to the full scale system within engineering accuracy.
Attitudes toward Korean Americans
박수현,Marilyn A. Cornish,Mijin Chung,Jingyo Suh 한국상담학회 2018 Journal of Asia Pacific counseling Vol.8 No.2
The objective of the current study is to understand Americans’ attitudes towards Koreans in the communities to which Koreans and Korean Americans have moved in increasing numbers over the last 15 years. Three hundred fifty- nine non-Koreans residing in the Southeastern region of the United States participated in the study. We conducted a cross-sectional survey study by using a modified version of the Attitudes toward Asians (ATA) Scale originally developed by Ho and Jackson. The results of this study validate the results of some previous studies investigating attitudes of Americans towards Asian Americans. However, the results also provide information that might be unique to Koreans/Korean Americans. Counseling, educational, and research implications as well as limitations were discussed.