경계성 난소 종양의 보존적 수술 후 불임 환자에서 체외수정시술의 유용성

이현주,안가영,한호섭,박찬우,양광문,이인호,김태진,임경택,이기헌,강인수,Lee, Hyun-Joo,Ahn, Ka-Yougng,Hahn, Ho-Suap,Park, Chan-Woo,Yang, Kwang-Moon,Lee, In-Ho,Kim, Tae-Jin,Lim, Kyung-Taek,Lee, Ki-Heon,Kang, Inn-Soo 대한생식의학회 2007 Clinical and Experimental Reproductive Medicine Vol.34 No.2

목 적: 경계성 난소 종양 환자에 있어서 보존적 수술 후 임신에 성공한 군과 비임신 군간 환자의 특성에 차이가 있는지 알아 보았으며, 경계성 난소 종양의 보존적 수술 후 체외수정시술의 유용성에 대해 알아 보았다. 연구방법: 1995년 1월부터 1999년 10월까지 경계성 난소 종양으로 보존적 술을 시행 받은 가임 여성 가운데 임신에 성공한 군 (14명)과 비임신 군 (30명)간 환자의 특성에 차이가 있는지 알아 보았으며, 비임신 군 가운데 불임을 주소로 내원하여 체외수정시술을 시행 받은 5명의 10주기를 대상으로 체외수정시술 결과 및 체외수정시술 후 난소 종양의 재발 유무를 추적 관찰하였다. 결 과: 경계성 난소 종양의 보존적 수술 후 임신에 성공한 군과 비임신 군간 환자의 평균 나이는 25.9세와 27.0세이었으며 혈청 CA-125 수치는 59.7 U/m와 72.0 IU/ml로 비임신 군에서 높은 경향을 보였으나 통계적 유의성은 없었다. 불임을 주소로 체외수정시술을 시행 받은 5명의 10주기 (attempt IVF cycle) 가운데 취소된 2주기를 제외하고 (attempt cycle cancellation rate, 20.0%) 채취된 난자 수는 평균 5.6개 (2$\sim$16개)이었으며 수정율은 14.5% (50.0$\sim$100.0%)이었다. 평균 2.4개 (1$\sim$4개)의 배아를 이식하였으며 배아이식 주기당 임신율과 착상율 및 출생율은 각각 50.0% (4/8 cycle), 31.6% 및 50.0% (4/8 cycle)이었다. 체외수정시술 후 평균 29.6개윌 (14$\sim$61개월)의 추적 관찰 기간 동안 난소 종양이 재발된 경우는 없었다. 결 론: 경계성 난소 종양의 보존적 수술 후 임신에 성공한 군과 비임신 군간 환자의 특성에 차이가 없으며, 보존적 수술 후 체외수정시술의 결과는 비교적 만족할 만한 수준으로 체외수정시술은 경계성 난소 종양의 보존적 수술후 고려될 수 있으나 향후 많은 환자를 대상으로 한 장기간의 추적 관찰을 필요로 한다. Objective: To examine determinants of successful pregnancy and evaluate COH-IVF outcomes of infertile patients after conservative surgical treatment of borderline ovarian tumor (BOT). Methods: In women of BOT (n=93), from January 1995 to December 1999, 44 of 93 women underwent conservative surgical treatment. From theses 44 women, patients characteristics, surgical and histological parameters were compared between 14 women who conceived and 30 women who failed to conceive. For 5 infertile women of 30 women who failed to conceive, 10 attempt IVF cycles were analysed; clinical pregnancy rate (CPR), implantation rate (IR) and live birth rate (LBR). Results: Women who conceived tend to be younger (25.9 vs 27.0 years) and lower serum CA-125 level (59.7 vs 72.9) compared to women who failed to conceive without significant difference. For 8 cycles out of 10 attempt IVF cycles, except for 2 cancellation cycles, the mean number of oocytes retrieved was 5.6 (range 2$\sim$16) with a mean fertilization rate of 74.4%. The CPR, IR and LBR per embryo transfer were 50.0% (4/8 cycles), 31.6% (6/19) and 50.0% (4/8 cycles) respectively. During the mean follow-up period after COH-IVF initiation, 29.6 (range 14$\sim$61) months, no recurrence was found. Conclusion: No determinant of successful pregnancy was found after conservative treatment for BOT. COH-IVF may be considered for infertile patients after conservative treatment of BOT. However, larger clinical studies with longer follow-up are necessary to evaluate the safety and efficacy of COH-IVF. All patients should be informed of the potential risks associated with ovarian hyperstimulation and close follow-up is necessary after COH-IVF.

해방 후 李承燁의 ‘統一民族國家’ 건설운동(1945~1953)

이현주(Lee, Hyun-Joo) 인천대학교 인천학연구원 2007 인천학연구 Vol.6 No.-

It was through the process of a merger of three parties that Lee Seung-Yeop rose as a leader of the socialist state movement in South Korea. The inauguration ceremony of the South Korean Worker's Party (남조선노동당) was held under his leadership in November, 1946 after Park Hun-young went to North Korea. He visited secretly PeyongYang accompanied by Kim Sam-yong and achieved the principal that the Opposing Parties individually had to join the South Korean Worker's Party established by centralizing the Korean Communist party. After crossing over the border into North Korea, Lee Seung-Yeop was appointed as a head of judicature of the First Cabinet, North Korea. However, his role was prominent in the Parties. Lee Seung-Yeop rose to the position supervising operations against the South as second secretary of the Party as well as was elected an member of Nine Politics Committeemen of the Korean Workers' Party(조선노동당) which was born due to the merger of Parties. Since then, his activities were concentrated on the leadership for guerrilla war by a partisan. He reorganized a guerrilla unit into three groups in order to develop systematically the guerrilla that was being sporadically progressed in South Korea in July, 1947 and the first group was dispatched to the South in his order. The Korean War was a watershed deciding his fate. He who sent to the South as plenipotentiary of liberated area as soon as the war occurred suddenly was nominated a representative of Seoul city of the Armed Services Committee, North Korea and Seoul temporary Council of People's Commissars the minute that Seoul was occupied. He, an chairman of Council People's Commissars, had to support the People's Army advancing South, recovering ruined product facilities, roads, bridges etc. The management for relief programs, citizens' mobilization, letting volunteer armies go to the war, cultural propaganda programs, land reform, health movement, product power increase was encouraged for about two months. Orders for a guerilla continued. At the same time, Lee Seung-Yeop made people sympathize with the People's Army by infiltrating espionage operators each province, landing the mustered guerrilla to the South through the East Sea on June 25, 1950. Even though his power decreased in the situation an armistice was argued, the first half of 1951, his leadership to the guerrilla was well. The end of the war with Incompletion brought a tragedy to him. Lee Seung-Yeop was arrested with other 11 fellows of the South Korean Worker's Party faction on August 3, 1952. He disappeared in the North, 'a forever strange land', after being sentenced to death and property confiscation upon a quick trial for 4 days, on August from 3 to 6, 1953. The event was not ended in Lee's death. The Purge against the South Korean Worker's Party continued until 1955 and became an overture of the purge against Soviet Union Group(소련파) and Yeon Ahn Group(연안파). His tragic death was not resulted from 'conspiracy of armament riots for the plot against government', but the fact he couldn't clean himself of suspicion even in North Korea, not Kim Il-Sung but Pak Hung-Young whom he assisted as the chief until he died, even if shouting many times 'General Kim Il Sung cheers!' at the official meeting. He failed to free himself from 'a second man of the South Korean Worker's Party until the last moment.

추황배(Pyrus pyrifolia Nakai cv. Chuhwangbae) 과피로부터 1종의 Sterol과 3종의 배당체 화합물의 단리·동정

이유건(Yu Geon Lee1),조정용(Jeong-Yong Cho),이현주(Hyun Joo Lee),이용현(Yong Hyun Lee),이상현(Sang-Hyun Lee),한태호(Tae-Ho Han),김월수(Wol-Soo Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.5

본 연구에서는 배의 유용성 증명을 위한 일환으로 배의 화학성분을 분자수준에서 밝히고자 하였다. 이에 배 과피 MeOH 추출물을 용매분획하여 얻은 EtOAc-산성 분획과 EtOAc-중성분획을 대상으로 Sephadex LH-20, silica gel, 그리고 ODS colmn chromatography와 HPLC를 이용하여 정제 및 단리하였다. 그 결과, EtOAc-산성 분획과 EtOAc-중성 분획으로부터 각각 2종씩의 화합물을 단리하였다. 단리된 화합물 1-4는 1H- 및 13C-NMR 분석을 통하여 각각 (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (화합물 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone(piceoside, 화합물 2), β-sitosterol (화합물 3), 그리고 β-sitosteryl 3-O-β-D-glucopyranoside (화합물 4)로 동정되었다. 단리된 3종의 배당체 화합물(화합물 1, 2, 4)들은 본 연구에 의해 배로부터 처음 동정되었으며, 화합물 3은 추황배로부터 처음 동정되었다. 본 연구결과가 배 함유 성분연구는 물론 배의 기능성 해명 연구에도 추후 중요한 기초자료로 활용되길 기대한다. We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.

광역학요법 후 발생한 맥락막비관류로 인한 급격한 시력저하 1예

이현주,강지은,이정희,Hyun-Joo Lee Ji-Eun Kang Jeong-Hee Lee 대한안과학회 2005 대한안과학회지 Vol.46 No.8

Purpose: We report a case of visual recovery in 11 weeks after acute, severe visual acuity decrease without significant fundus change following photodynamic therapy (PDT) for occult choroidal neovascularization (CNV) related to age-related macular degeneration. Methods: A 79-year-old female patient visited our clinic because of decreased vision which had developed 1 week previously in her left eye. Best corrected visual acuity was 0.4 in the left eye. On fundus examination, there were multiple drusen around the fovea, hyperpigmentation and irregular elevation of retinal pigment epithelium, and serous pigment epithelium detachment on the macula. Fluorescein angiogram showed stippled hyperfluorescence with indistinct border in the early phase and hyperfluorescence due to the leakage from CNV of diffuse type ooze in the late phase. ICG angiogram showed focal hyperfluorescence in the mid and late phases. PDT with verteporfin was performed for regression of CNV. The day after PDT, visual acuity decreased to counting fingers at 20 cm and on fundus examination, there was no increase of serous pigment epithelium detachment or subretinal hemorrhage, but only mild retinal edema in the inferonasal area to the fovea. Fluorescein angiogram and ICG angiogram showed only round-shaped hypofluorescence due to choroidal nonperfusion localized to the PDT site. Eleven weeks after PDT, choroidal circulation returned to normal and visual acuity was 0.6. Results: Acute, severe visual acuity decrease after PDT can be developed by choroidal nonperfusion and can be recovered with the improvement of choroidal perfusion.

집단검진 환자에서 측정한 안구회선의 범위

이현주,임기환,Hyun-Joo Lee,M,D,Key-Hwan Lim,M,D 대한안과학회 2005 대한안과학회지 Vol.46 No.10

Purpose: To determine the range of cyclotorsion in the mass screening of patients by studying the spatial relationship between the optic disc and the fovea. Methods: We examined the horizontal and vertical distances from the center of the optic disc to the fovea using the fundus photographs of 249 subjects (498 eyes) without a past history of strabismus through mass screening and then calculated the optic disc-foveal angle. We studied the differences in all the measured parameters between the right and left eyes and between men and women. Results: The horizontal distance from the center of the optic disc to the fovea was 2.59±0.26 DD (disc diameter) in the right eye and 2.58±0.27 DD in the left eye. The mean horizontal distance was 2.59±0.26 DD. The vertical distance from the center of the optic disc to the fovea was 0.28±0.14 DD in the right eye and 0.30±0.13 DD in the left eye. The mean vertical distance was 0.29±0.14 DD. The calculated disc-foveal angle was 6.26±2.98 degrees in the right eye and 6.69±2.84 degrees in the left eye (mean, 6.50±2.92 degrees). There were no significant differences between the right and left eyes or between males and females. The 95% range of the location of the fovea was from 0.01 DD to 0.57 DD inferior to the optic disc center. Conclusions: We concluded that the clinical location of the fovea, as measured in the mass screening, extends from the center of the optic disc to the lower edge of the optic disc.

말초혈액 자연살해세포가 증가된 반복유산 환자의 탈락막 자연살해세포의 발현

이현주,김선행,양광문,박동욱,박찬우,홍성란,조동희,송인옥,궁미경,강인수,Lee, Hyun-Joo,Kim, Sun-Haeng,Yang, Kwang-Moon,Park, Dong-Wook,Park, Chan-Woo,Hong, Sung-Ran,Cho, Dong-Hee,Song, In-Ok,Koong, Mi-Kyoung,Kang, Inn-Soo 대한생식의학회 2009 Clinical and Experimental Reproductive Medicine Vol.36 No.3

목 적: 말초혈액 자연살해세포가 증가된 반복유산 환자군과 증가되지 않은 반복유산 환자군에서 다음 번 유산 시탈락막의 자연살해세포의 발현양상을 관찰하여 말초혈액 자연살해세포와 상관관계가 있는지 보고자 하였다. 연구방법: 말초혈액 자연살해세포가 15% 이상 증가된 반복유산 환자군 14명을 실험군으로, 15% 이하인 군 12명을 대조군으로 하여 연구를 진행하였다. 이 환자들에게서 다음 번 유산 시 착상부위를 포함한 자궁 탈락막 세포를 확인하여 면역조직화학염색을 통해 $CD56^+$와 $CD16^+$ 자연살해세포의 수를 세어 실험군과 대조군을 비교하였다. 결 과: 실험군과 대조군은 탈락막 내 $CD56^+$ 자연살해세포의 수 간에 통계적으로 유의한 차이 ($170.1{\pm}132.1$ vs. $68.3{\pm}66.1$, p=0.02)를 보였으나 둘 간의 상관관계 (r=0.229, p=0.261)는 없었다. 또한 두 군간에 $CD16^+$ 자연살해세포는 통계적으로 유의한 차이 ($25.70{\pm}11.72$ vs. $31.17{\pm}22.67$)를 보이지 않았다. 결 론: 본 연구는 말초혈액 $CD56^+$ 자연살해세포가 증가된 반복유산 환자군에서 말초혈액 자연살해세포가 증가되지 않은 군에 비하여 탈락막의 $CD56^+$ 자연살해세포가 증가되어 있음을 알 수 있었다. 이는 탈락막 자연살해세포가 반복유산의 면역학적 기전에서 중요한 역할을 할 것이라는 가능성을 제시해 주는 연구 결과이며 또한 말초혈액 자연살해세포의 측정이 반복유산 환자에서 다음 번 유산을 예측할 수 있는 유용한 지표임을 알 수 있다. Objectives: The purpose of this study was to compare the decidual NK cell populations between increased pre-conceptional peripheral NK cell population and normal pre-conceptional peripheral NK cell population in women with a history of recurrent abortion. Methods: Fourteen women with history of recurrent abortion and elevated pre-conceptional peripheral NK cell, above 15% of peripheral lymphocyte population were included in this study. As a control, twelve women with history of recurrent abortion and their peripheral NK cell percentage showed below 15% were included. Distribution of $CD56^+$ and $CD16^+$ NK cells in paraffin embedded decidual tissues including implantation sites were examined by immunohistochemical staining using anti-CD56, 16 monoclonal antibodies. After immuohistochemical staining, the numbers of decidual NK cells were counted and compared these results between study and control groups. Results: There was significant difference in decidual $CD56^+$ NK cell count ($170.1{\pm}132.1$ vs. $68.3{\pm}66.1$, p=0.02) between increased peripheral $CD56^+$ NK cell group and control group. But, there showed no statistically significant correlation between decidual $CD56^+$ NK cell count and peripheral $CD56^+$ NK cell percentage (r=0.229, p=0.261). Also there was no statistically difference decidual $CD16^+$ NK cell count between study and control group ($25.70{\pm}11.72$ vs. $31.17{\pm}22.67$), and no correlation between decidual $CD16^+$ NK cell and peripheral $CD16^+$ NK cell percentage (r=-1.40, p=0.535). Conclusions: This study shows that decidual $CD56^+$ NK cell are significantly increased in decidua of women exhibiting a history of recurrent abortion with increased $CD56^+$ peripheral NK cell. This study suggests that the percentage of peripheral NK cell reflect the expression of decidual NK cell. Consequently, pre-conceptional peripheral blood NK cell population can be the useful marker for detecting the risk of subsequent miscarriage.

실험계획법을 이용한 동축 에어블로운 전기방사 공정의 최적화

이현주,최민기,박진희,김주용,Lee, Hyun Joo,Choi, Min Ki,Park, Jin Hee,Kim, Jooyong 한국섬유공학회 2014 한국섬유공학회지 Vol.51 No.1

In this study, a coaxial air-blown spinning system has been optimized for the variables of air flow rate, solution concentration, and voltage using the design of experiment (DOE) method. Both response surface analysis (RSA) and Taguchi's method (TM) were employed and compared to obtain the optimal process conditions. A total of 41 spinning trials were utilized and fitted with RSA and TM; they were then used to obtain mathematical prediction models. The resulting optimum parameters were an air flow rate of 20 l/min, a concentration of 8 wt%, and a voltage of 35 kV, predicting an average diameter of 335.76 nm with an estimated error of 18.85 nm. In TM, the SN ratio was calculated on randomly chosen data. The optimal process conditions that gave the largest SN ratio were an air flow rate of 20 l/min, a concentration of 8 wt%, and a voltage of 30 kV, representing the estimated SN ratio and diameter, -52.03 and 309.11 nm, respectively. For coaxial air-blown electrospinning under the same conditions, the experimental SN ratio and diameter were -52.0269 and 376.75 nm, respectively.

난치성 녹내장에서 아메드 녹내장밸브 삽입술의 임상적 고찰

이현주,최규룡,Hyun Joo Lee,Kyu Ryong Choi 대한안과학회 2006 대한안과학회지 Vol.47 No.6

Purpose: To assess the efficacy, safety, and clinical outcome of the Ahmed glaucoma valve implant in refractory glaucoma. Methods: We retrospectively studied 55 eyes of 55 patients who underwent Ahmed glaucoma valve implantation for refractory glaucoma unresponsive to conventional management. The mean follow-up period was 26.3±11.5 months. Surgical success was defined as an intraocular pressure between 5 and 21 mmHg regardless of glaucoma medication. Results: The reduction of intraocular pressure was statistically significant (p<0.05) and the number of glaucoma medications was decreased. The 1-year and 2-year success rates were 83.0% and 75.8%, respectively. Complications included hyphema in 9 eyes, hypotony with shallow anterior chamber in 2 eyes, choroidal detachment in 4 eyes, tube obstruction in 1 eye, tube migration in 2 eyes, and bullous keratopathy in 2 eyes. Conclusions: Ahmed glaucoma valve implantation may provide good intraocular pressure control in patients with refractory glaucoma unresponsive to conventional treatments, and may be effective for reducing the incidence of hypotony in the early postoperative period.

여성 간호사의 근무형태 선호도에 따른 조직몰입 및 이직의도 비교연구

이현주,Lee, Hyun-Joo 대한산업경영학회 2021 산업융합연구 Vol.19 No.5

본 연구는 여성 간호사가 선호하는 근무형태에 따른 조직몰입도와 이직의도를 비교하여 여성 간호사의 근무 환경을 개선하고자 수행되었다. 본 연구는 서술적 조사연구로서 7개 의료기관에서 3교대 근무중인 여성 간호사 465명에게 온라인 설문조사를 통해 자료수집을 하였다. 수집된 자료는 SPSS WIN 24.0를 이용하여 χ² test, two sample independent t-test, pearson correlation, MANCOVA로 분석한 결과 고정근무 선호집단의 연령이 많을수록 조직몰입도가 높았으며, 이직경험이 있는 경우 이직의도가 높았다. 그리고 고정근무 선호집단이 교대근무 선호집단 보다 조직몰입도가 낮았으며 이직의도가 높았다. 고정근무를 희망하는 여성 간호사들의 조직몰입도를 높이고, 이직을 줄이기 위해서는 다양한 근무형태를 개발하여 여성 간호사의 근무환경을 개선하기 위한 노력이 필요하다. This study was performed to improve the working conditions of female nurses by comparing their organizational commitment and turnover intention according to the type of work shift they prefer. This study is a descriptive survey study, and data were collected by conducting an online survey of 465 female nurses working in three shifts at seven medical institutions. The collected data were analyzed using SPSS WIN 24.0 in a χ² test, a two-sample independent t-test, a pearson correlation and MANCOVA. The result shows that the group of nurses preferring fixed work shift had higher organizational commitment at higher ages and had higher turnover intention if they had an experience of transferring to another medical institution. The study also found that the group that prefers fixed work shift had lower organizational commitment and higher turnover intention than the group of nurses preferring shift work. The nursing department in hospital should strive to improve the working environment in female nurses by developing various types of work, with an aim to increase the organizational commitment of female nurses preferring fixed work and reduce their turnover.

분열효모에서 sphpr1 유전자의 결실이 생장 및 mRNA Export에 미치는 영향

이현주,윤진호,Lee, Hyun-Joo,Yoon, Jin-Ho 한국미생물학회 2012 미생물학회지 Vol.48 No.2

THOC1/Hpr1는 mRNA가 전사되는 동안 mRNP의 포장과 mRNA 방출에 관여하는 진화적으로 잘 보존된 THO 복합체의 한 소단위이다. 분열효모 Schizosaccharomyces pombe에서도 THOC1/Hpr1과 유사한 단백질을 암호화하는 유전자(sphpr1로 명명)를 찾아 그 특성을 조사하였다. 이배체 S. pombe 균주에 하나의 sphpr1 유전자만을 결실시킨 후 4분체 분석을 수행한 결과, 이 유전자는 생장에 필수적이었다. 티아민에 의해 발현이 조절되는 강력한 nmt1 프러모터를 이용하여 sphpr1를 과발현시키더라도 세포의 생장과 mRNA 방출에는 전혀 영향이 없었다. 하지만, sphpr1의 발현을 억제하면 생장이 억제되었으며 poly$(A)^+$ RNA가 핵 안에 축적되었다. 이와 같은 결과들은 sphpr1 유전자가 생장과 mRNA의 핵에서 세포질로의 방출에 관여하고 있음을 시사한다. THOC1/Hpr1 is one subunit of THO complex that is an evolutionally conserved assembly involved in the mRNP packaging and mRNA export during transcription elongation. In fission yeast Schizosaccharomyces pombe, an ortholog (spHpr1) of THOC1/Hpr1 was identified based on sequence alignment. A deletion mutant in a diploid strain was constructed by replacing one of spHpr1-coding region with a $kan^r$ gene using one-step gene disruption method. Tetrad analysis showed that the sphpr1 is essential for growth. Over-expression of sphpr1 from strong nmt1 promoter caused no defects of growth and mRNA export. However, repression of the sphpr1 expression resulted in growth inhibition accompanied by accumulation of poly$(A)^+$ RNA in the nucleus. These results suggest that spHpr1 is involved in mRNA export from the nucleus to cytoplasm.