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갈근에서 분리한 화합물의 $\alpha$-glucosidase와 $\alpha$-amylase 활성 저해 효과
박지희,백목련,이병회,연규환,유시용,김영섭,박상언,홍경식,Park, Jee-Hee,Baek, Mok-Ryeon,Lee, Byung-Hoi,Yon, Gyu-Hwan,Ryu, Shi-Yong,Kim, Young-Sup,Park, Sang-Un,Hong, Kyung-Sik 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.5
Compounds of isolated from roots extract of Pueraria thunbergiana were tested their inhibitory effects on $\alpha$-glucosidase and $\alpha$-amylase. Inhibitory activity of methylene chloride (MC) fraction and ethyl acetate (EA) fraction against $\alpha$-glucosidase showed more than 60% at a concentration of $500{\mu}g/m{\ell}$. Among the nine compounds tested on $\alpha$-glucosidase, biochanin A, (-)-tuberosin and calycosin from MC fraction and daidzein from EA fraction were stronger inhibitors than acarbose ($IC_{50}=530{\mu}g/m{\ell}$), and their $IC_{50}$ were 9, 144, 328 and $20{\mu}g/m{\ell}$, respectively. Biochanin A and (-)-tuberosin also inhibited $\alpha$-amylase activity as like as acarbose $IC_{50}=20.5{\mu}g/m{\ell}$), and their $IC_{50}$ were 22 and $348{\mu}g/m{\ell}$, respectively. Although daidzein was already known $\alpha$-glucosidase inhibitory effects, it was newly evaluated that biochanin A and (-)-tuberosin inhibited $\alpha$-glucosidase as well as $\alpha$-amylase, and that calycosin did $\alpha$-glucosidase.
김현기,최재석,유대석,최연희,연규환,홍경식,이병회,김혜진,김은주,박병근,정영철,김영섭,유시용 한국생약학회 2007 생약학회지 Vol.38 No.2
A rapid and practical HPLC assay was developed for quantitative analysis of saponins in Platycodi Radix. Seven saponin components in Platycodi Radix, i.e., deapioplatycoside E, platycoside E, deapioplatycodin D3, platycodin D3, polygalacin D2, platycodin D2 and platycodin D were successfully resolved on C18 column and detected by ELSD. Standard curves were linear over the concentration range 1~2,000 μg/ml (r 2 >0.992). Intra- and inter-day coefficients for variation of seven saponin components were <10% and limit of quantification of them were around 0.7~1.5 μg/ml, respectively. Using this method, contents of seven saponins in various plant materials under different cultivating conditions were estimated.
최춘환,Joo-Won Yoon,연규환,김영섭,유시용,석순자,Sunny Kang,김영호 한국생약학회 2012 Natural Product Sciences Vol.18 No.4
Mushrooms have a long history of use in traditional medicine, and hundreds of novel constituents in mushrooms with miraculous biological properties have been identified recently. Although diverse effects for medicinal use of mushrooms such as anticancer activity are proven, their reversal activities of drug resistance in cancer cells was rarely reported so far. In the search for novel medicinal use of mushrooms, we tested the multidrug resistance (MDR) reversal activities of diverse mushrooms collected from Korea. Among, the mushroom extracts tested, Cantharellus cibarius (M02) and Russula emetica (M12) revealed MDR reversal activities of paclitaxel in the P-glycoprotein (Pgp)-positive HCT15 and MES-SA/dX5 cancer cells, but not in the Pgp-negative A549 and MES-SA cancer cells. In addition, these mushrooms also enhanced the cytotoxicity of doxorubicin, another well-kwown Pgp-associated anticancer drug against MES-SA/DX5 cells, but not against MES-SA cells. Meanwhile, the cytotoxicity of cisplatin, a well-known Pgp-non-associated anticancer drug, was not affected by the mushrooms all the cells tested. From these results, we suspected that some ingredients of M02 and M12 have Pgp-associated MDR reversal activities.
Streptomyces scopuliridis KR-001의 분리 동정 및 잡초 방제효과
이보영,김재덕,김영숙,고영관,연규환,김창진,구석진,최정섭 한국잔디학회 2013 Weed & Turfgrass Science Vol.2 No.1
With increasing environmental issues from synthetic chemical herbicides, microbe-originated herbicides could be a fascinating alternative in current agriculture. We isolated Streptomyces strains that produced herbicidal active metabolite(s) against a grass weed Digitaria sanguinalis. According to the result from 16S rDNA sequence comparison with the close strains, the best isolate (Code name MS-80673) was identified as Streptomyces scopuliridis KR-001. The closest type strain was Streptomyces scopuliridis RB72 which was previously reported as a bacteriocin producer. The optimal culture condition of S. scopuliridis KR-001 was 28oC, pH 7.0 and culture period 4 to7 days. Both of soil and foliar application of the crude culture broth concentrate was effective on several troublesome or noxious weed species such as a Sciyos angulatus in a greenhouse and field condition. Phytotoxic symptoms of the culture broth concentrate of S. scopuliridis KR-001 by foliar application were wilting and burn-down of leaves, and stems followed by discoloration and finally plant death. In crops such as rice, wheat, barley, hot pepper and tomato, growth inhibition was observed. These results suggest that the new S. scopuliridis KR-001 strain producing herbicidal metabolites may be a new bio-herbicide candidate and/or may provide a new lead molecule for a more efficient herbicide. 토양 방선균 유래의 천연 제초활성 후보소재를 발굴하고, 이 후보소재의 온실 및 포장 실증 평가를 통해 보다친환경적이고 효율적인 잡초 관리를 위한 잡초방제제로의실용화 가능성을 검토하고자 본 연구를 수행하였다. 3,000여 종의 토양 방선균으로부터 강력하고 독특한 제초활성을 발현하는 후보 방선균 2종을 선발하였으며, 선발된 후보 방선균의 16S rDNA 서열을 분석한 결과 S. scopuliridisRB72와 가장 유사도가 높아 선발 균주를 S. scopuliridisKR-001이라 명명하였다. 후보 방선균의 최적 배양조건을확립하였고, 온실 및 포장조건에서 주요 문제잡초와 난방제 잡초, 환경 위해 잡초 가시박 방제효과를 평가를 통해친환경적인 천연물 제초제로서의 개발 가능성을 확인하였다. 한편, 후보 방선균 S. scopuliridis KR-001 배양액은 작물에 대한 선택성은 없었기 때문에 비선택성 경엽처리제로의 개발 가능성을 고려한 처리 농도 및 시기 등에 대한진전단계 연구를 진행할 필요성이 제기되었다. 향후 배양액 수준에서 제형 연구를 통해 약효증진기술과 대량생산성 기술 개발을 통해 실용화 가능성을 검토하는 한편, 배양액 내의 살초성분에 대한 화학구조 구명을 통해 방선균유래의 천연 제초제를 개발 할 수 있는 선도물질 발굴을위한 추가 연구가 필요한 것으로 판단하였다.