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스트론튬(Strontium)이 도핑된 다공성 BCP 뼈 이식제가 조골세포에 미치는 영향
변인선,서형석,이병택,송호연,Byun, In-Seon,Sarkar, Swapan Kumar,Seo, Hyung-Seok,Lee, Byong-Taek,Song, Ho-Yeon 한국재료학회 2010 한국재료학회지 Vol.20 No.3
In this study, we investigated primary biocompatibility and osteogenic gene expression of porous granular BCP bone substitutes with or without strontium (Sr) doping. In vitro biocompatibility was investigated on fibroblasts like L929 cells and osteoblasts like MG-63 cells using a cell viability assay (MTT) and one cell morphological observation by SEM, respectively. MTT results showed a cell viability percent of L929 fibroblasts, which was higher in Sr-BCP granules (98-101%) than in the non-doped granules (92-96%, p < 0.05). Osteoblasts like MG-63 cells were also found to proliferate better on Sr-doped BCP granules (01-111%) than on the non-doped ones (92-99%, p < 0.05) using an MTT assay. As compared with pure BCP granules, SEM images of MG-63 cells grown on sample surfaces confirmed that cellular spreading, adhesion and proliferation were facilitated by Sr doping on BCP. Active filopodial growth of MG-63 cells was also observed on Sr-doped BCP granules. The cells on Sr-doped BCP granules were well attached and spread out. Gene expression of osteonectin, osteopontin and osteoprotegrin were also evaluated using reverse transcriptase polymerase chain reaction (RT-PCR), which showed that the mRNA phenotypes of these genes were well maintained and expressed in Sr-doped BCP granules. These results suggest that Sr doping in a porous BCP granule can potentially enhance the biocompatibility and bone ingrowth capability of BCP biomaterials.
Morphology of the Dart and the Dart Sac in the Land Snail Nesiohelix samarangae
변인선,정계헌,이용석,조용훈 한국패류학회 2004 The Korean Journal of Malacology Vol.20 No.1
An anatomical and ultrastructural study on the dart sac and the dart of a Korean snail Nesiohelix samarangae was conducted to understand their morphological characteristics with the help of the light microscope and electron microscopes, TEM and SEM.Nesiolelix samarangae had two darts which are pure white structures 6-8 mm in length, tapering from 0.6-0.8 mm to 0.15-0.3 mm. The dart sac had a long conic lumen subdivided into two by a septal wall, and the darts were centrally embedded in the thick muscular layers of the sac. The darts occupied each of the two luminal spaces one per each.The convexed surfaces of the darts had many crystal buds in the shape of the petals. Otherwise, the convexed surfaces of the darts had numerous crystal buds in the shape of candle or topaz. The luminal surface of the dart sac was covered with a single columnar epithelium. The epithelial cells possessed microvilli on their free surface.
대식세포가 β-tricalcium Phosphate 뼈이식제의 생분해에 미치는 영향
김영희,변인선,오익현,민영기,양훈모,이병택,송호연,Kim, Young-Hee,Jyoti, Anirban,Byun, In-Sun,Oh, Ik-Hyun,Min, Young-Ki,Yang, Hun-Mo,Lee, Byong-Taek,Song, Ho-Yeon 한국세라믹학회 2008 한국세라믹학회지 Vol.45 No.10
Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of $\beta$-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders $\beta$-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type $\beta$-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with $\beta$-TCP nano powders synthesized by microwave-assisted process. We confirmed the phagocytosis of osteoclasts by observing $\beta$-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the $\beta$-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type $\beta$-TCP synthesized by fibrous monolithic process. The $\beta$-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type $\beta$-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of $\beta$-TCP bone graft substitutes.
바지락 (Ruditapes philippinarum) 의 중금속 축적에 관한 연구
이용석,조용훈,변인선,강세원,조은미,한연수,최상행,박홍석,고원규,안인영,정계헌 한국패류학회 2006 The Korean Journal of Malacology Vol.22 No.2
The present study was conducted to confirm that a bivalve Ruditapes philippinarum can be used as a biomarker for the monitoring of the heavy metal pollution in the silt of the marine environment. The clams were collected from the silt of Cheonsu-bay, Buheung-ri, and Tan-island of the West Sea, Korea. To observe the normal structures of the target organs (hepatopancreas and gill), they were dissected out for the immunohistochemical study and the electron microscopy with TEM, SEM, and SEM-EDS. The immunohistochemical study showed that the interdiverticular connective tissues of the hepatopancreas, and the outer epithelium of the gill lamellae was strongly reacted to anti-metallothionein (MT), indicating the presence of MT, a metal-binding protein, involved in metal detoxifying process. According to the examinations under the TEM, the epithelial cells of the hepatopancreas of the clams collected from polluted area (Tan-island) showed certain changes such as swollen rER, swollen nuclear envelope and inclusion bodies in the nulcei. In the SEM-EDS analysis, tissue of the hepatopancreas showed relatively higher concentration of S, Zn, and Cd. These elements are supposed to be concerning with the MT-reaction in the hepatopancreas. Considering that the coastal bivalve R. philippinarum showed immediate subcellular responses to heavy metal pollution in the overall experiments conducted, this species might act as one of efficient biomarkers for the heavy metal contamination in the marine environment.
왼돌이물달팽이(Physa acuta)의 Metallothionein 유전자 클로닝 및 발현양상
조용훈,백문기,강세원,이재봉,변인선,최상행,채성화,강정하,한연수,박홍석,이용석 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.3
Metallothioneins (MTs) play a key role in metallic homeostasis and detoxification in most living organisms. In an attempt to study the biological functions and significance of MT in a snail, we cloned and partially characterized the MT gene from the left-handed snail, Physa acutawhich has been regarded as a potential biomonitering species for fresh water. The complete cDNA sequence of PaMT cDNA was identified from the expressed sequence tag (EST) sequencing project of Physa acuta. The coding region of 180 bp gives 60 amino acid residues including the initiation methionine and termination codon. Clustering and phylogenic analysis of PaMT with other MT amino acid sequences show that it has some identities to Helix pomatia (60%), Arianta arbustorum (58%), Perna viridis (49%), Mytilus edulis (49%), Bathymodiolus azoricus (49%), Bathymodiolus azoricus (48%) and Bathymodiolus sp. FD-2002 (48%). Time dependent induction for PaMT from P. acuta exposed with cadmium (50 ppb) indicated that PaMT was induced at 4-8 hr after exposure. It remains to further develop PaMTas a potential biomarker for water contamination in fresh water.
Effects of Macrophage on Biodegradation of β-tricalcium Phosphate Bone Graft Substitute
김영희,Anirban Jyoti,변인선,오익현,민영기,양훈모,이병택,송호연 한국세라믹학회 2008 한국세라믹학회지 Vol.45 No.10
Various calcium phosphate bioceramics are distinguished by their excellent biocompatibility and osteoconductivity. Especially, the exceptional biodegradability of β-TCP makes it a bone graft substitute of choice in many clinical applications. The activation of osteoclasts, differentiated from macrophage precursor cells, trigger a cell-mediated resorption mechanism that renders β-TCP biodegradable. Based on this evidence, we studied the biodegradation process of granular-type β-TCP bone graft substitute through in vitro and in vivo studies. Raw 264.7 cells treated with RANKL and M-CSF differentiated into osteoclasts with macrophage-like properties, as observed with TRAP stain. These osteoclasts were cultured with β-TCP nano powders synthesized by microwaveassisted process. We confirmed the phagocytosis of osteoclasts by observing β-TCP particles in their phagosomes via electron microscopy. No damage to the osteoclasts during phagocytosis was observed, nor did the β-TCP powders show any sign of cytotoxicity. We also observed the histological changes in subcutaneous tissues of rats implanted with granule-type β-TCP synthesized by fibrous monolithic process. The β-TCP bone graft substitute was well surrounded with fibrous tissue, and 4 months after implantation, 60% of its mass had been biodegraded. Also, histological findings via H&E stain showed a higher level of infiltration of lymphocytes as well as macrophages around the granule-type β-TCP. From the results, we have concluded that macrophages play an important role in the biodegradation process of β-TCP bone graft substitutes.
이상룡,오경석,이건호,설상영,변상준,윤혜경,고원규,김오길,전진숙,김학준,안용준,하준호,김순관,전인선,김 형 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.1
A 69-year-old male was admitted to the neurosurgery department for traumatic intracra-nial hemorrhage in both frontal lobes. After 2 months, he complained of epigastric dis-comfort, nausea, vomiting, and loose stools. The gastric endoscopic examination found acute hemorrhagic gastritis and there were rhabditoid nematode larvae in the gastric fluid and biopsy sections. The filariform larvae of Strongyloides sp. were discovered from a fecal culture. The patient was treated with albendazole (200 mg, po bid, for 4 weeks). The epigastric discomfort disappeared and endoscopic findings improved after treatment.