Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Immunity to melanoma mediated by 4‐1BB is associated with enhanced activity of tumour‐infiltrating lymphocytes

Ju, Seong‐,A,Lee, Sang‐,Chul,Kwon, Tae‐,Hyoung,Heo, Sook‐,Kyoung,Park, Sang‐,Min,Paek, Ha‐,Na,Suh, Jae‐,Hee,Cho, Hong Rae,Kwon, Byungsuk,Kwon, Byoung S,Kim, B Nature Publishing Group 2005 Immunology and Cell Biology Vol.83 No.4

<P>4‐1BB costimulates T cells to carry out effector functions such as eradication of established tumours. 4‐1BB (CD137) is a member of the TNF receptor family, and its triggering by either 4‐1BB ligand or antibody ligation induces T‐cell activation and growth. We analysed tumour‐infiltrating lymphocytes (TIL) in the experimental B16F10 melanoma model to determine the mechanisms involved in 4‐1BB‐mediated tumour suppression. 4‐1BB<SUP>+/+</SUP> mice survived longer than 4‐1BB<SUP>–/–</SUP> mice, and survival was further prolonged by triggering 4‐1BB with an agonistic mAb. The number of metastatic B16F10 colonies in the lung was much greater in 4‐1BB<SUP>–/–</SUP> mice than in their 4‐1BB<SUP>+/+</SUP> littermates. Administration of agonistic anti‐4‐1BB mAb increased the number of TIL in the tumour masses in the lungs of 4‐1BB<SUP>+/+</SUP> mice. The numbers of CD4<SUP>+</SUP> T, CD8<SUP>+</SUP> T and CD11b<SUP>+</SUP> TIL increased in these mice. Anti‐4‐1BB mAb induced not only CD8<SUP>+</SUP> 4‐1BB<SUP>+</SUP> T cells but also a CD8<SUP>+</SUP> IFN‐γ<SUP>+</SUP> T‐cell population. B16F10 cells from the lungs of anti‐4‐1BB‐treated mice showed enhanced expression of MHC class Ι and II antigens compared with the same cells from control IgG‐treated mice. Thus, the increase in number of CD8<SUP>+</SUP> T cells and enhanced MHC Ι and II expression in B16F10 cells that result from augmented IFN‐γ production in response to anti‐4‐1BB mAb may lead to suppression of tumour growth and metastasis.</P>

The inability of Bacillus licheniformis perR mutant to grow is mainly due to the lack of PerR-mediated fur repression

Kim, J. H.,Yang, Y. M.,Ji, C. J.,Ryu, S. H.,Won, Y. B.,Ju, S. Y.,Kwon, Y.,Lee, Y. E.,Youn, H.,Lee, J. W. MICROBIOLOGICAL SOCIETY OF KOREA 2017 JOURNAL OF MICROBIOLOGY -SEOUL- Vol. No.

<P>PerR, a member of Fur family protein, is a metal-dependent H2O2 sensing transcription factor that regulates genes involved in peroxide stress response. Industrially important bacterium Bacillus licheniformis contains three PerR-like proteins (PerR(BL), PerR2, and PerR3) compared to its close relative Bacillus subtilis. Interestingly, unlike other bacteria including B. subtilis, no authentic perR (BL) null mutant could be established for B. licheniformis. Thus, we constructed a conditional perR (BL) mutant using a xylose-inducible promoter, and investigated the genes under the control of PerR(BL). PerR(BL) regulon genes include katA, mrgA, ahpC, pfeT, hemA, fur, and perR as observed for PerR(BS). However, there is some variation in the expression levels of fur and hemA genes between B. subtilis and B. licheniformis in the derepressed state. Furthermore, katA, mrgA, and ahpC are strongly induced, whereas the others are only weakly or not induced by H2O2 treatment. In contrast to the B. subtilis perR null mutant which frequently gives rise to large colony phenotype mainly due to the loss of katA, the suppressors of B. licheniformis perR mutant, which can form colonies on LB agar, were all catalase-positive. Instead, many of the suppressors showed increased levels of siderophore production, suggesting that the suppressor mutation is linked to the fur gene. Consistent with this, perR fur double mutant could grow on LB agar without Fe supplementation, whereas perR katA double mutant could only grow on LB agar with Fe supplementation. Taken together, our data suggest that in B. licheniformis, despite the similarity in PerR(BL) and PerR(BS) regulon genes, perR is an essential gene required for growth and that the inability of perR null mutant to grow is mainly due to elevated expression of Fur.</P>

Variations in DBTT and CTOD within weld heat-affected zone of API X65 pipeline steel

Ju, J.B.,Kim, W.s.,Jang, J.i. Elsevier Sequoia 2012 Materials science & engineering. properties, micro Vol.546 No.-

<P>The fracture resistance of heat-affected zones (HAZs) in girth welded joint of API X65 steel pipeline was systematically investigated. While the change in Charpy impact energy has been typically evaluated in previous studies, here the variations in ductile-to-brittle temperature (DBTT) and crack-tip opening displacement (CTOD) within HAZ were explored. A series of experiments revealed that both values vary dramatically (i.e., DBTT increases and CTOD decreases) as the location approaches the fusion line (FL) and thus the region adjacent to FL exhibited the lowest CTOD and highest DBIT, possibly due to the increasing portion of coarse-grained HAZ. Interestingly, however, even the FL regions still showed moderate toughness at -40 degrees C similar to room temperature. Microstructural analysis and additional impact tests using simulated HAZ specimens suggested a possibility that fine-grained HAZs with higher toughness may suppress the brittle fracture from neighboring coarse-grained region. (C) 2012 Elsevier B.V. All rights reserved.</P>

Genetic diversity and natural selection of Duffy binding protein of Plasmodium vivax Korean isolates

Ju, H.L.,Kang, J.M.,Moon, S.U.,Bahk, Y.Y.,Cho, P.Y.,Sohn, W.M.,Park, Y.K.,Park, J.W.,Kim, T.S.,Na, B.K. Verlag für Recht und Gesellschaft ; Elsevier 2013 Acta tropica Vol.125 No.1

Plasmodium vivax Duffy binding protein (PvDBP) is a micronemal type I membrane protein that plays an essential role in erythrocyte invasion of merozoites. PvDBP is a prime blood stage vaccine candidate antigen against P. vivax, but its polymorphic nature represents a major obstacle to the successful design of a protective vaccine against vivax malaria. In this study, we analyzed the genetic polymorphism and natural selection at the N-terminal cysteine-rich region of PvDBP (PvDBPII) among 70 P. vivax isolates collected from Korean patients during 2005-2010. Seventeen single nucleotide polymorphisms (SNP), which resulted in 14 non-synonymous and 3 synonymous mutations, were found in PvDBPII among the Korean P. vivax isolates. Sequence analyses revealed that 13 different PvDBPII haplotypes, which were clustered into 3 distinct clades, were identified in Korean P. vivax isolates. The difference between the rates of nonsynomyous and synonymous mutations suggested that the region has evolved under natural selection. High selective pressure preferentially acted on regions identified or predicted to be B- and T-cell epitopes and MHC binding regions of PvDBPII. Recombination may also contribute to genetic diversity of PvDBPII. Our results suggest that PvDBPII of Korean P. vivax isolates display a limited genetic polymorphism and are under selective pressure. These results have significant implications for understanding the nature of the P. vivax population circulating in Korea and provide useful information for development of malaria vaccines based on this antigen.

“Grafting-From” Polymerization inside a Polyelectrolyte Hollow-Capsule Microreactor

Choi, Won San,Park, Jeong-Ho,Koo, Hye Young,Kim, Ju-Young,Cho, B. K.,Kim, Dong-Yu WILEY-VCH Verlag 2005 Angewandte Chemie Vol.44 No.7

<B>Graphic Abstract</B> <P>Interior decorating: Polymerization of styrene sulfonate monomers on the inner surface of polyelectrolyte hollow capsules results in polymers having molecular weights an order of magnitude higher than those produced in solution. The shape of the capsules after polymerization can be manipulated by varying the styrene sulfonate content (see SEM images; a: 25 wt %, b: 40 wt %). <img src='wiley_img/14337851-2005-44-7-ANIE200460971-content.gif' alt='wiley_img/14337851-2005-44-7-ANIE200460971-content'> </P>

간호대학생의 영적안녕과 정신건강이 영적간호역량에 미치는 영향

조은주B(Eun-Ju CHO),조은주A(Eun-Joo JO) 한국상담심리교육복지학회 2021 상담심리교육복지 Vol.8 No.6

본 연구는 간호대학생의 영적안녕과 정신건강이 영적간호역량에 미치는 영향을 알아보고자 시도 되었다. B시 소재 K대학의 간호학과 3, 4학년 학생 116명을 대상으로 자료를 수집하였고, 자료수집 기간은 2020년 10월 5일에서 8일까지이었다. 자료분석은 SPSS WIN 20.0 program을 이용하여 기술 통계, t-test, ANOVA, Scheffé test, Pearson s correlation coefficients, multiple regression으로 분석 하였다. 연구결과, 영적간호역량과 영적안녕 및 영적안녕의 하위요인인 종교적 안녕과 실존적 안녕 은 양의 상관관계가 있었고, 영적간호역량과 정신건강도 양의 상관관계가 있었다. 영적간호역량에 가장 큰 영향을 미치는 요인은 정신건강이었으며 다음으로 영적안녕과 종교기간 순이었고, 설명력은 29.4%이었다. 따라서 간호대학생의 영적간호역량을 증진하기 위해서는 정신건강 증진 프로그램과 영적안녕 중재프로그램의 개발이 필요하다. This study was conducted to identify the influence of Spiritual Well-being, Mental Health on Spiritual Care Competence in Nursing Students. The subjects were 116 nursing college students in B city. Data were collected from Oct. 5th to 8th, 2020 through the survey of the participants. Then, this study statistically processed the data using the t-test, ANOVA, Scheffe’s test, Pearson’s correlation coefficient analysis and the multiple regression analysis in accordance with the SPSS WIN 20.0 Program. There was a significant positive correlation between Spiritual Care Competence and Spiritual Well-being. Also, there was a significant positive correlation between Spiritual Care Competence and Mental Health. The meaningful variables which influence the Spiritual Care Competence were Mental Health, Spiritual Well-being and period of religion in order. These factors were responsible for 29.4% of the total variance in Spiritual Care Competence in Nursing Students. In conclusion, to increase the Spiritual Care Competence of nursing students, it is necessary to develop Mental Health promotion program and Spiritual Well-being intrevention program.

Protective effects of Bacillus subtilis against Salmonella infection in the microbiome of Hy-Line Brown layers

Oh, Ju Kyoung,Pajarillo, Edward Alain B.,Chae, Jong Pyo,Kim, In Ho,Kang, Dae-Kyung Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

Objective: This study investigated the effects of Bacillus subtilis CSL2 (B. subtilis CSL2) administration before Salmonella challenge on the fecal microbiota and microbial functionality of Hy-line Brown (HLB) laying hens. Methods: Fecal samples were collected from control (CON), Salmonella-infected (SAL) and Salmonella-infected, probiotic-treated (PRO) groups before and after Salmonella challenge for microbiome analysis using 16S rRNA gene pyrosequencing. Results: Infection with Salmonella led to decreased microbial diversity in hen feces; diversity was recovered with Bacillus administration. In addition, Salmonella infection triggered significant alterations in the composition of the fecal microbiota. The abundance of the phylum Firmicutes decreased while that of Proteobacteria, which includes a wide variety of pathogens, increased significantly. Bacillus administration resulted in normal levels of abundance of Firmicutes and Proteobacteria. Analysis of bacterial genera showed that Salmonella challenge decreased the population of Lactobacillus, the most abundant genus, and increased populations of Pseudomonas and Flavobacterium genera by a factor of 3 to 5. On the other hand, Bacillus administration caused the abundance of the Lactobacillus genus to recover to control levels and decreased the population of Pseudomonas significantly. Further analysis of operational taxonomic units revealed a high abundance of genes associated with two-component systems and secretion systems in the SAL group, whereas the PRO group had more genes associated with ribosomes. Conclusion: The results of this study indicate that B. subtilis CSL2 administration can modulate the microbiota in HLB laying hens, potentially acting as a probiotic to protect against Salmonella Gallinarum infection.

Differential cytotoxic effects of sodium meta-arsenite on human cancer cells, dental papilla stem cells and somatic cells correlate with telomeric properties and gene expression.

Jeon, Byeong-Gyun,Kumar, B Mohana,Kang, Eun-Ju,Maeng, Geun-Ho,Lee, Yeon-Mi,Hah, Young-Sool,Ock, Sun-A,Kwack, Dae-Oh,Park, Bong-Wook,Rho, Gyu-Jin Potamitis Press 2011 Anticancer research Vol.31 No.12

<P>We investigated the effects of sodium meta-arsenite (NaAsO(2)) on human cancer cells (MDA-MB-231, MCF-7 and U-87 MG), dental papilla tissue stem cells (DPSCs) and somatic cells [MRC-5 fetal fibroblasts and adult muscle cells (MCs)] by examining telomeric properties, endogenous reverse transcriptase (RT) activity and the expression of tumorigenesis-linked genes. Half maximal inhibitory concentration (IC(50)) values were higher in DPSCs and MCs, possessing longer telomere lengths when compared to cancer cells. Levels of telomerase and RT activity, and the expression of protein 53 (p53), B-cell lymphoma 2 (BCL2), nuclear factor kappa-light-chain-enhancer of activated B-cells (NFκB), transforming growth factor beta (TGFβ) and vascular endothelial growth factor (VEGF) were significantly lower in cancer cells following sodium meta-arsenite treatment, whereas the effect was absent or marginally detected in DPSCs and somatic cells. Collectively, sodium meta-arsenite effectively induced cellular cytotoxicity by inhibiting telomerase and RT activity, and down-regulating transcript levels in cancer cells with shorter telomere lengths, whereas more tolerance was evident in DPSCs and somatic cells possessing longer telomere lengths.</P>

전라북도 아동의 예방접종실태에 관한 조사연구

정주미,김정철,은소희,황평한,김정수,Choung, Ju Mi,Kim, Jung Chul,Eun, So Hee,Hwang, Pyoung Han,Nyhambat, B.,Kilgore, P.,Kim, Jung Soo 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.10

목 적: 전라북도지역의 어린이 예방접종의 실태를 파악함으로써 문제점을 알아보고 예방접종률과 접종 시기의 적절성을 향상시키고자 하였다. 방 법: 2000년 3월부터 6월까지 전북지역의 5세 이하의 소아 850명을 대상으로 하였으며 조사방법은 보호자의 면담과 예방접종수첩 등 의무기록을 근거로 이루어 졌다. 예방접종 종류에 따른 접종여부, 접종시기와 횟수, 접종장소에 대해 조사하였다. 결 과 : 1) 조사방법은 전체 850명 중 362명(43%)은 예방접종수첩을 통해 이루어졌으며, 488명(57%)은 보호자의 기억을 통한 면담으로 이루어졌다. 2) 전체 예방접종의 50.4%는 보건소에서, 44.3%는 개인의원에서, 5.3%는 대학병원과 종합병원에서 이루어졌다. 기본접종인 BCG(49%), B형 간염(47%), DTaP(61%), MMR(55%), 일본뇌염(73%)은 보건소에서 주로 접종되었고, 수두(62%)와 Hib백신(88%)등 선별접종은 개인의원에서 더 많이 이루어졌다. 3) 예방접종률은 BCG는 99.2%, B형 간염은 93.5%, DTaP & TOPV는 96.1%로 비교적 접종률이 높았으나, 선별접종인 MMR은 83.7%, 수두는 72.5%, 일본뇌염은 50.2%, Hib는 15.8%로 낮은 접종률을 보였다. 4) 예방접종 시기의 적절성은 B형간염은 88.4%, DTaP는 72.8%, 일본뇌염은 18.5%로 각각의 접종률인 93.5%, 94.6%, 50.2%와 큰 차이를 보였다. 결 론 : 1세 미만에 실시하는 예방접종들의 접종률은 비교적 높았으나 1세 이후에 실시하거나, 여러 차례 추가접종이 필요한 경우는 접종률과 적절성이 모두 낮았다. 특히, MMR, 수두, 일본뇌염, Hib 백신의 경우 지역사회에서의 유행을 예방하기에는 아직 낮은 수준으로 평가된다. 이러한 예방접종률을 높이기 위해서는 무엇보다도 부모들의 백신 접종에 대한 인식을 높이는 것이 중요하고, 예방접종기록을 표준화하고 전산화하며, 예방접종 기록을 초등학교 입학시에 제출하는 것을 의무화하는 것이 바람직할 것이다. 그리고, 예방접종 사업에 대한 평가와 이를 환류 할 수 있는 감시체계가 필요할 것으로 생각된다. Purpose : This study was carried out to obtain the vaccination rate and age appropriateness of vaccination in children under five years of age in Jeonbuk province. Methods : Eight hundred and fifty infants and children were enrolled in this study. Vaccination rate(Number of vaccinees/Number of subjects), places of vaccination, age appropriateness of vacination were examined by either vaccine record review or interview with parents or guardians. Results : The salient features of the findings were as follows : All subjects were aged under five and mostly under two years of age(68.8%). Places of vaccination were health centers or subcenters( 50.4%), private clinics(44.3%), and general hospitals(5.3%). Routine vaccinations such as BCG, hepatitis B vaccine(HBV), diphtheria, tetanus, acellular pertussis(DTaP) and Trivalent Oral Polio Vaccine(TOPV), measles, mumps, rubella(MMR), and Japaneses B encephalitis(JBE) were vaccinated mostly in health centers or subcenters, while chickenpox(CHP) and haemophilus influenzae vaccines(Hib), which are not routine in Korea, were vaccinated in private clinics. The vaccination rates of BCG(99.2%), HBV(93.5%) and DTaP(96.1%) were very high. But those of MMR(83.7%), CHP(72.5%), JBE(50.2%), and Hib(15.8%) were lower than expected. Considering the age appropriateness of vaccination, some infants and children were not appropriately vaccinated(vaccination rate/age appropriateness of vaccine; HBV, 93.5%/88.4% : DTaP, 94.6%/73.1% : JBE, 50.2%/ 18.5%). Conclusion : The vaccination rate of BCG, HBV, DTaP and TOPV was very high, but MMR, CHP, JBE, and Hib vaccination rate was not high enough to be able to protect against epidemic. We should pay more attention to vaccinating children, and there is a need for a program that will enhance coverage for vaccines.