형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

농촌 집단검진시 재검수진 행위이행에 관한 연구 : Papanicolau 세포진 검사를 중심으로 In Case of Papanicolau Smear

유지소,염용태 고려대학교 의과대학 1992 고려대 의대 잡지 Vol.29 No.3

Among females in Korea, cervical cancer is one of the most common malignant tumors Exfoliative cytology is a highly sensitive and specific test with low expense for early detection of cervical cancer and its precursors. This is why the test is widely used for screening purpose. However, in case of screening, the validity is lowered, and so it should be compensated for by recheck to confirm. From the viewpoints above, the Korea University performed prescheduled cervical cancer screening tests from 1987 to June 1990 for a total of 1,761 women living in Yeoju Gun, Kyong Kee Province where K.U. Hospital is located. This research includes the results of the tests and the discussion on the participation in second stage examination for the confirmation purpose. This can be summarized as follows. 1. Cytological result of the Papanicolau smear revealed 0.7% of C.Ⅲ and 0.1% of C.Ⅳ category. And 42.2% of cases showed some signs of gynecological inflammation which were subjected to recheck. 2. A total of 209 (27.9%) among 750 inflammatory category participated in the second stage confirmation test. 3. It was confirmed that geographical accessibility in terms of distance from hospital to residential area could not afford to influence the rate of participating in confirmation test. 4. As the result of stepwise multiple regression analysis of the subjects to be rechecked. accumulative R square of symptom level. occupation, economic status, age and numbers of children was 46.8% in relation to the participation in the second stage examination for confirmation purpose.

Treatment of Radioactive Liquid Waste by the Forced-AirCirculation System

Tae-κuk Kim,IL Sik Kang,Jong-Sik Shon,Young-Gerl Ryu,Won-Hyuk Jang,Sang-Min Bae,Young-Yong Ji,Dae-Seok Hong,Kyung-Kil Kwak 한국방사성폐기물학회 2011 한국방사성폐기물학회 학술논문요약집 Vol.9 No.2

近視性 屈折異常의 疫學的 硏究

劉世煥,廉容泰 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

The distribution of ametropia whether refractive or axial in communities is variable according to factors such as ethnicon, age, living environment, and working begavior among others. Among young adults in Korea whose eves are fully matured most ametropia results form myopic refractive errors. It is well known that the prevalence of myopia among college students in Korea is very high, more than 60%. Nevertheless, community based epidemiological research on the causalities of myopia has: not been adequately performed to provide preventive or control measures for myopia for the young in Korea. In view of this, the author attempted an epidemiological study on myopia using the distribution of personal characteristics, and analyzing these factors on a total of 8,555(4,006 in 1981 and 4,549 in 1982) newly enrolled college students of Korea University The summarized results were as follows: 1. A total sample of 115 among 3,337 ametropic cases discovered during screening by visual acuity examination were selected for confirmation by objective refraction tests. One hundred percent of these were discovered to be of the myopic type. Statistical analysis of visual acuity and refraction revealed: R=-3.172+3.407V, r=-0.781, p<0.001 V=0.748+0.179R, r=-0.781, p<0.001 Using simple linear regression; and log(-R)=0.543-1.050V, R^(2)=0.673, F=419.478, p<0.001 logV=-0.042+0.629R, R^(2)=0.760, f=588.559, P<0.001 using logarithmic transformation of exponential curve linear regression, where V= visual acuity, and R=refraction in diopter. 2. The prevalence of ametropia was 65% in 1981 and 73% in 1982. Most of the ametropic cases were predicted to be the myopic type of refractive errors. No significant difference in prevalence by sex was found. However, the rates among males raised in urban areas, and among high economic females found to be significantly higher than other groups. 3. Factors related to causalities of myopia were analysed in terms of work credit, reading behavior, heredity, and food habits as shown in the followings; (1) The correlation between the variables of visual acuity and score on college entrance examinations was V=-0.01S+1.52, r=-0.14, p<0.001(where S=score) Showing a low interpretation capacity but very high reliability. (2) Average distance between the eyes and a book while reading was significantly shorter in myopia than the emetropia group. Distance in the majority of myopia cases were less than 25cm. (3) Attack rate of myopia of siblings born from myopic parents(one or both eyes) was found to be significantly higher(doubled) than that from emetropic parents. This provides substantial evidence that heredity plays an important role in revealing myopia. (4) There were more vegetarians and persons with seriously unbalanced diets in the myopia group. However, rates by visual acuity were too low to be tested for significance. 4. Clinical findings included the relationship between the site of major eye and myopic eye, data on the correction of visual acuity, and onset on visual disturbances. (1) In the majority of the emetropic group the major eye was the right eye, while in persons whose right eyes were myopic the majority had left eyes as the major ones. Similarly, in case where the left eye was myopic the right eye tended to be the major eye. In cases where both eyes were myopic, the pattern followed the emetropic group in that the majority had the right eyes as the major eye. (2) The more severe the myopic status was, the higher the rate of wearing glasses was. About 50% of glasses wearers got their glasses from opticians without an ophthalmologist’s prescription. (3) The onset of visual disturbance in the majority(47%) of myopic cases was during highschool.

단면의 성질을 적용한 크기와 회전 변화에 불변인 영상 검사 시스템

이용중,김태원,김기대,류재엽 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

The purpose of this paper is to develop image inspection system endows an automatic operating and measuring that the moment values are invariant with respect to variable object size and rotation. In this paper, using these moment feature vector with Hu's 7 invariant moment is also given. The charasteristics of section which is applied in the mechanics used moment descriptor of invariant moment detection algorithm for image inspection system. Corresponding rates between 94% and 96% have been achived for all object tested.

미국의 식물유래약용물질(Plant-Made Pharmaceuticals, PMPs)의 안전성 규제

김태산,원소윤,이근표,류태훈,진용문,이길복,김현준 한국국제농업개발학회 2005 韓國國際農業開發學會誌 Vol.17 No.1

1. 식물유래 약용물질 (PMP)은 살아있는 식물에서 생산된 치료용 단백질로, 발효조에서 세포계통을 이용하는 것보다 적은 비용으로 높은 순도의 단백질을 생산할 수 있어 이에 대한 연구개발이 확대될 전망이다. 2. 식물유래 약용물질의 재배시험을 감독하는 주관부서는 미농무부(USDA)의 동식물 검역청(APHIS)이다. 3. PMP의 생산에는 알팔파. 옥수수, 벼 잇꽃과 담배 등이 이용되고 있다. 4. 현재 상업화된 PMP는 없으나 ProdiGene, Inc사 등 몇몇 생명공학 회사에서 이들의 연구개발이 진행되고 있다. 5. 2002년에 PMP 재배시험은 130에이커 규모로 34개 시험장에서 20건이 허가 되었으며 주별로는 애리조나, 네브라스카 등 14주에서 시험이 이루어 졌다. 6. 미국 USDA의 PMP 재배 지침에 의하면 PMP의 재배생산에는 안전성확보를 위한 별도의 전용장비를 필요로 하며 파종에서 생산까지 엄격하게 관리된다. 7. 미국 USDA의 APHIS는 2002년 PMP 옥수수를 생산하는 ProdiGene, Inc사에 대하여 2건의 포장시험허가 위반사항을 적발하여 벌금 조치를 한바있다. Plant-made pharmaceuticals(PMPs) are therapeutic agents(pharmaceutical proteins) produced in genetically engineered plants. To yield these valuable products plants offer several advantages that include large-scale production capacity at a reduced cost, equivalent purity/activity to other manufacturing systems, and freedom from contamination with animal pathogens, prions, or disease-causing germs. But there are risks of contamination of the food supply and unintended effects on ecosystems. In the U.S. PMPs are regulated under stringent requirement of the U.S. Department of Agriculture(USDA) and the Food and Drug Administration(FDA). In this article, contamination incident with PMP corn engineered by ProdiGene, Inc will be briefly introduced and regulations of PMPs will be discussed.

地方職化 以後 農村指導事業의 推進 方向

황대용,류진춘,하태일 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

As a result of the execution of the import liberalization and local autonomy with WTO system launching, centralized rural extension service converted to localized rural extension service. This advent of globalization and localization era affects our over all agricultural condition, moreover the duty and role of rural extension organization which take complete of rural extension, is compelled to diversified and specified technology. In this study, first we will investigate the change of agricultural, rural community, and local rural extension service in globalization and localization era. Second we will analyze the confronted problems of local rural extension service. With this study's outcome for basis, we will present new role and direction of local rural extension service which can contribute to local agricultural development.

DBD 반응기에서 플라즈마 방전형태에 따른 PFC_(s) 가스의 분해 특성

김관태,김용호,차민석,송영훈,김석준,류정인 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.5

Perfluorocompounds(PFC_(s)), such as tetrafluoromethane (CF₄) and hexafluoroethane (C₂F_(6)), have been widely used as plasma etching and chemical vapor deposition (CVD) gases for semiconductor manufacturing processes. Since these PFC_(s) are known to cause a greenhouse effect intensively, there has been a growing interest in reducing PFC_(s) emissions. Among various CF₄ decomposing techniques, a dielectric barrier discharge (DBD) is considered as one of a promising candidate because it has been successfully used for generating ozone (O₃) and decomposing nitrogen oxide (NO). Firstly, optimal concentration of oxygen for CF₄ decomposition was found to figure out how many primary and secondary reactions are associated with DBD process. Secondary, to find effective discharge method for CF₄ decomposition, a streamer and a glow mode in DBD are experimentally compared, which includes (ⅰ) coaxialcylinder DBD, (ⅱ) DBD reactor packed with glass beads. and (ⅲ) a glow mode operation with a helium gas. The test results showed that optimal concentration of oxygen was ranged 500 ppm~1% for treating 500 ppm of CF₄ and helium glow discharge was the most efficient one to decompose CF₄.

알루미늄 연속주조 용탕의 탈 가스 일체화 장치 개발

이용중,김태원,김기대,류재엽,이형우 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

It is necessary for managing a perfect process for degasing aluminum molten metal according to the increase of a grade of aluminum and its alloy products. There are some methods that have been used to manage a degasing process in recent years, such as an injection method that uses aluminum molten metal powder and chemicals supplier and input method that supplies argon and nitrogen, or chlorine gas by using a gas blow-tube. However, these methods show some problems, and it shows that it is a difficult process to handle, pollution due to the producing a lot of toxic gases like chlorine and fluoride gas, irregular effects, and lowering work efficiency due to the excessive processing time. The problems that are the most fatal are the producing a lot of sludge due to the reaction of aluminum molten metal with chemicals, loss of metals, and decreasing the life of refractory materials. In order to solve these problems, this paper develops a technology that is related to aluminum continuous casting molten metal and monolithic degasing apparatus. A degasing apparatus developed in this study improved the existing methods and prevented environmental pollution with smokeless, odorless, and harmlessness by using a new method that applies argon and nitrogen gas in which the methods used in the West and Japan are eliminated. The developed method can significantly reduce product faults that are caused by the production of gas and oxidation because it uses a preprocessed molten metal with chemicals. In addition, the amount of the produced sludge can also be reduced by 60~80% maximum compared with the existing methods. Then, it makes it possible to minimize the loss of metals. Moreover, the molten metal processing and settling time is also shortened by comparing it with the existing methods that are applied by using chemicals. In addition, it does much to improve the workers' health, safety and environment because there is no pollution. The improvement of productivity and prevention effects of disaster from the results of the development can be summarized as follows. It will contribute to the process rationalization because it does not have any unnecessary processes that the molten metal will be moved to an agitator by using a ladle and returned to process for degasing like the existing process due to the monolithic configuration. There are no floating impurities due to the oxidation caused by the contact with the air as same as the existing process. In addition, it can protect the blending of precipitation impurities. Because it has a monolithic configuration, it can avoid the use of additional energy to compensate the temperature decreasing about 60? that is caused by the moving of molten metal. It is not necessary to invest an extra facilities in order to discharge the gas generated from a degasing process by using an agitator. The working environment can be improved by the hospitable air in the factory because the molten metal is almost not exposed in the interior of the area.

해양미생물로부터 카로테노이드 색소의 생산을 위한 최적조건

정영기,박정욱,주우홍,류병호,정명주,김태수 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The optimal medium composition for the production of carotenoid pigment from Haloarcular sp. EH-1 as a dietary for fishes were 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate(pH7.0). The incubation temperate, aeration rate and agitation speed were 40℃, 100ml medium/500ml vol. shaking flask, and 180 rpm/min. The carotenoid pigment production was highest after 5 days of incubation with the light.