Rat 전지 상완의 근섬유형 구성에 대한 연구

정명래,김홍선,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

This study was to determine the fiber-type populations and calculate the cross-sectional areas in each fiber-type in the muscle of the brachium of the rat. For 20 rats, the flexors (brachialis and biceps brachii) and extensors (triceps brachii) of the elbow taken from dissection. The fibers were classfied histochemically as fast-twitch oxidative glycolytic fiber(FOG), fast-twitch glycolytic fiber (FG), or slow-twitch oxidative fiber(SO), and their populations were determined. The cross-sectional areas of each type muscle fibers were calculated with microcomputer image analyzer. The results were as follows ; I. The variations in fiber-type population of various muscles with which their positions were observed as well as those reported in ot4er studies. 2. In both extensor and flexor groups, the deep region of the muscles was consisted of higher populaions of SO fibers, while more superficial regions of the same muscles were more FG fibers. 3. The percentage of SO fibers were larger in the extensors than that of flexors. 4. Except the SO fibers, the cross-sectional areas of FOG and FG fibers in the extensors were larger than that of flexors.

SEK1 카이네이즈 과발현(overexpression)생쥐 대식세포주(RAW264,7)의 Nitric Oixde(NO)유도성 세포고사 (apoptosis) 기전에 관한 연구

정병학,소홍섭,박래길,정헌택 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Nitric oxide (NO) induces apoptotic cell death in murine RAW 264.7 macrophages. To A elucidate the NO-induced apoptotic mechanisms in SEK1/MKK4 overexpressed RAW 264.7 cells, we generated clones of RAW 264.7 cells which stably overexpressd kinase inactive SEK1 (RAW/SEK1-KI) or wild type SEK1 (RAW/SEK1-WT). Treatment of kinase inactive SEK1 transfected RAW 264.7 cells (RAW/SEK1-KI) with sodium nitroprusside (SNP), a NO donor, significantly decreased the cell viability than that of RAW control cells which were treated with the same amount of SNP. However, RAW/SEK1-WT cells were less susceptible to NO induced apoptosis. Furthermore, the treatment of NO with farnesyltransferase inhibitor (FTI) of Ras or MEK inhibitor (PD098059) significantly increased the apoptotic death of RAW/SEK1-KI. However, SB203580, a specific p38 inhibitor, did not affect NO-induced apoptosis of kinase inactive SEK1 transfected RAW 264.7 cells. For a while, caspase 3-like protease activity in NO plus FTI treated RAW/SEK1-KI cells were more increased than that of NO only. In addition, nuclear transcription factor kB (NFkB) was significantly activated in NO-treated RAW/SEK1-KI cells, whereas these transcriptional factor was not markedly activated in NO-treated RAW/SEK1-WT cells. Supershift analysis demonstrates that NFkB was composed of mainly p50 homodimer. Also pyrrolidine dithiocarbamate (PDTC), a strong inhibitor of NFkB, significantly inhibits the NO-induced apoptosis in RAW/SEK1-KI cells. Taken together, we suggest that SEK1 may play anti-apoptotic role in RAW cells from NO-induced apoptosis via the modulation of NFkB. In addition, in the absence of SEK1 kinase cascade activation, the viability of RAW cells may be mainly dependent on Ras/Raf/MEK/ERK pathway.

유전자 알고리즘을 이용한 이동로봇의 지능제어

정동연,김홍래,한성현 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

This paper proposed trajectory tracking control of Mobile Robot. Trajectory tracking control scheme are Real coding Genetic-Algorithm and Back-propergation Algorithm. Control scheme ability experience proposed simulation.

모바일 로봇의 견실제어를 위한 제네틱 알고리즘 개발

김홍래,배길호,정경규,한성현 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

This paper proposed trajectory tracking control of mobile robot. Trajectory tracking control scheme are real coding genetic-algorithm and back-propergation algorithm. Control scheme ability experience proposed simulation. Stable tracking control problem of mobile robots have been studied in recent years. These studies have guaranteed stability of controller, but the performance of transient state has not been guaranteed. In some situations, constant gain controller shows overshoots and oscillations. So we introduce better control scheme using Real coding Genetic Algorithm(RCGA) and neural network. Using RCGA, we can find proper gains in several situations and these gains are generalized by neural network. The generalization power of neural network will give proper gain in untrained situation. Performance of proposed controller will verify numerical simulations and the results show better performance than constant gain controller.

청각장애자 운동지도를 위한 프로그램 고찰

김정수,박정래,노일환,원충희,김흥식,강신일,박찬홍,김달영,이철원,이찬규 公州大學校스포츠科學硏究所 1987 스포츠科學硏究所論文集 Vol.- No.1

Hearing impairement in varying degrees constitutes one of the most common disabilites affecting school aged children. Eight out of every 1,000 students under the age of twenty have significant hearing loss. Hearing disabilities range from slight to total loss of sound perception. Statistics indicate that only one out of every ten hearing impaired children will become deaf. Most motor activities can be included students may possess low levels of static and/ or dynamic balance, but the greatest majority possess normal motor and physical capabilities. All the hearing impaired youngsters should be properly assessed and given the identical testing batteries as normal children. Hearing impaired students have, essentialy, a communication handicap and suffer from inability to understand verbal instructions. All physical educators should be able to use minimal finger spelling and signing for hearing impaired students.

Primary Empty Sella 증후군 1례

이홍준,손일진,정은경,문경래,박상기,박영봉 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

The pimapty sella syndrome results from and extension of the subarachnoid space into and intrasellar position with subsequent remodeling of the sella turcica and flattening of the pituitary gland. We experienced a case of primary emty sella syndrome in a 14-year-old male patient who was hospitalized because of short stature and micropenis. The endocrinologic studies revealed growth hormone and gonadotropin hormone deficiencies. The magnetic resonance imaging showed sella turica filled with CSF and flattened pituitary glad. The patient was followed-up with growth hormone therapy and the result was favorable.

초등학교용 웹 기반 교수-학습 자료 개발 전략

김홍래,정인기,신준식,이대형 춘천교육대학교 과학교육연구소 2001 과학교육연구 Vol.25 No.-

두개하악장애환자의 임상적 평가를 위한 컴퓨터적외선체열검사 : CLINICAL EVALUATION

조익주,홍정표,이상래 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to assess the thermographic differences of craniomandibular area between normal indivisuals and patients with craniomandibular disorders and to compare the differences between clinical and thermographical assessment. The author had used 50 subjects as materials for this study, which was divided into 2 groups (first group included 15 healthy subjects and second group included 35 patients) with craniomandibular disorders; 17 subjects had normal disc-condyle relationship, 13 subjects had disc displacement with reduction and 5 subjects had disc displacement without reduction. Agema870 thermovision(D.I.T.I.) was used to take thenuographs with 0.1t difference of gradual temperature shift. The. results were as follows 1. Of 34'patients with craniofacial pain, 15(44.1%) subjects showed hyperthermia on the pain site in the theiniography, 8(23.5%) exhibited hyperthermia on the site opposed to the pain site, and 11(32.4%) did not show any significant thermal change. One patient without craniofacial pain showed hyperthermia on the site opposed to the site of disc displacement without reduction. 2. Of 35 patients with craniofacial pain or disc dispalcement, 24(68.6%) subjects showed a significant thermal difference between symptomatic and asymptomatic sides of the face, but 11(31.4%) did not show any difference. 3. Of 17 patients with pain but with normal disc-condyle relationship, 8(47.1%) subjects- showed hyperthermia on the pain site, 4(23.5%) showed on the site opposed to the pain site, and 5(29.4%) did not show any significant thermal change. 4. Of 13 patients with pain and disc dispacement with reduction, 6(46.2%) subjects showed hyperthermia on the pain site, 3(23.1%) showed on the site opposed to the pain site, and 4(30.8%) did not show any significant thermal change. 5. 15 healthy subjects did not show any thermal differences between the both sides of the face.

형질변형성장인자가 손상골 재생능에 미치는 영향에 과한 실험적 연구

이병도,황의환,이상래,홍정표 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the effect of TGF-81, which promotes differentiation and proliferation of osteoblasts, on bone regeneration. Experimental bone defects that measured 3 mm in diameter were created on the mandibles of guinea pig by removal of bone with the use of trephine burs. In one side of mandibular body, the experimental groups, bone defects were grafted with Biogran(Orthovita Co., U.S.A.) and TGF 131(R&D System Co., U.S.A.). In the remaining side of the mandiblar body, the control groups, bone defects were grafted with only Biogran. Guinea pigs in the control and experimental groups were serially terminated by fours on the 3 days, the 1 week, the 2 weeks, the 3 weeks, and the 4 weeks after experiment, and both sides of the mandibular bodies were removed and fixed with 10% neutral formalin. They were decalcified and embedded in paraffin as using the usual method. The specimen sectioned and stained with hematoxylin and eosin. Also, they were radiographed with a soft X-ray apparatus. The Obtained results.=were as follows; 1. Hemorrhagic condition, observed in the granulation tissues, disappeared on the 1 week after experiment in both groups, and more prominent in the experimental group. The granulation tissues of the experimental group had larger number of cells than those of the control group. 2. Osteoblastic differentiation in the margin of grafted material and adjacent bone was observed on the 1 week after experiment in both groups. Also, bone formation was observed in immature form on the 1 week after experiment, and more prominent in the experimental group. 3. In the polarizing microscopic examination, bone matrix was very loose on the 1 week after experiment, but increase in density with time, and more prominent in the experimental group. 4. In the microradiographic examination, newly formed bone was observed in the experimental group on the 2 weeks after experiment, and this was observed earlier than in the control group. Newly formed bone was increased with time and defected area was markedly decreased on the 4 weeks after experiment.

Streptozotocin유도 당뇨병이 백서하악 절단과두 치유에 미치는 영향의 실험적 연구

황의환,이상래,홍정표,김원철 大韓口腔顎顔面 放射線學會 1994 Imaging Science in Dentistry Vol.24 No.2

The purpose of this study was to investigate the remodeling process of the streptozotocin-induced diabetic rat's resected condyle. This experiment was performed with male Sprague-Dawly strain rats weighing approximately 250gm, which were rendered diabetic by an intravenous injection of streptozotocin(70 ㎎/㎏ body weight). After condylectomy, experimental rats were serially terminated on the 1st week, the 2nd week, the 3rd week, and the 4th week. The following termination, the mandibles were dissected out to make specimens. Each mandibular condyle was radiographed with Hitex HA-80(Hitex Co., Ltd. Japan). In addition to radiographic observation, the mandibular condyles, further decalcified and embedded in paraffin, were sectioned and stained with Hematoxylin and Eosin, Toluidine blue and Masson's trichrome. The were observed with a light microscope and a polarizing microscope. The results were as follows. 1.Soft X-ray radiograms revealed proliferation of bone after 1 week in both groups. Irregularly repaired bones and dense trabeculae were clearly observed in experimental group. 2.The resected condyles were repaired by intramembraneous and endochondral bone formation in both groups. 3.Bone tissue repair was initiated from the adjacent margin of resected bone, and cartilginous tissues were observed at the top of repaired one in both groups. 4.The number of osteoblasts of experimental group was small, compared with control group. Each osteoblast was small and flap. The thin trabeculae were irregularly formed. 5.Collagens of bone were gradually matured in both groups, but the degree of maturation was lower in experimental group. 6.Fibrous tissues covered the upper parts of repaired bone were densely arranged in the both groups. Conclusively, atrophied osteoblasts, immature collagen of bone, and this and irregular trabeculae which were characterized in the diabetes experimental group showed diabetes disturbed osteoblastic function and caused disturbance of remodeling process of bone.