ICP-AES를 이용한 대기분진 중 중금속 분석에 대한 정도관리

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2003 環境硏究 Vol.21 No.-

In this study, factors of quality control such as laboratory environment, capability of instrument/equipment, method of analysis, calibration, and standard solution were investigated to obtain quality assurance when analyzing airborne heavy metals (e.g., Pb, Cr, Cd, Cu, Mn, Ni, Fe) using ICP-AES. A procedure of quality assurance and evaluation method of uncertainty are also suggested by applying ISO Guide. The suggested process of quality assurance will be helpful for the national monitoring network to produce better data.

폐철을 이용한 크롬(VI)의 환원적 제거

임우택,정용식,유건상,김종현,김영훈 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

Cr(VI) is a known toxic heavy metal which is found frequently in many industrial sites. Contaminates soil and ground water with Cr(VI) is a big enviromental concern due to the high toxicity. Permeable Reactive Barrier(PRB)is an innovative remedial technology intensively studied and developed recenty. The most popular PRB material is zero-valent iron which is environmentally favorable and cheap. Scrapped steels and precleaned scrapped steels were tested in this study for Cr(VI). Scrapped steels were precleaned wuth detergent, acetone, hexane.The precleaned scrapped steel showed relatively high reactivity for Cr(VI). The results indicate that the scrapped steel could be used as PRB material after proper pretreatments.

관상동맥질환의 중증도와 혈중지질치 및 아포지단백과의 상관관계

임현주,황종현,류재근,정병천,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

목적 : 관상동맥질환의 위험인자로서 혈청지질 및 혈장 지단백에 대한 관심이 높아지고 있고 또 아포지단백이 관상동맥 질환을 일으키는데 다른 지질보다 더 중요하다는 연구가 많이 발표되고 있으나 우리나라에서는 아포지단백에 대한 연구가 그리 많지 않은 편이다. 이에 저자등은 혈중지질치와 새로운 위험인자로 알려지고 있는 아포지단백치를 측정하고 이들과 관상동맥질환 중증도와의 상관 관계를 알아보았다. 대상 및 방법 : 관상동맥질환이 의심되어 관상동맥조영술을 실시한 69명의 환자를 대상으로 하였으며 12시간 이상 공복후 혈청을 채취하여 총콜레스테롤, 중성지방, HDL-콜레스테롤, 아포지단백 AI (ape AI), 아포지단백 B (ape B)를 측정하였다. 관상동맥조영상 50%이상의 협착이 있는 혈관의 수 (lesion number), 최대협착을 합한 값 (lesion score), 각 분절의 최대협착을 합한 값 (total score), 가장 심한 협착 값 (peak stenosis) 등으로 관상동맥 중증도를 알아본 다음 이들과 혈청지질치, 아포지단백과의 상관 관계를 비교해 보았다. 상관 관계는 Spearman's correlation coefficient를 구하였다. 결과 : 관상동맥질환의 중증도와 각 지질치 간의 상관 관계를 비교해 보았을때 lesion number와 관계있는 것은 연령 (r=0.2789), 중성지방치 (r=0.2829)이며 lesion score와 관계있는 것은 연령(r=0.2911), 중성지방치0 (r=0.3316), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2361), apo B 치(r=0.2759), apo AI과 apo B의 비 (-0.3154)로 나타났다. Total score는 연령 (r=0.3100), 총콜레스테롤치 (r=0.2452), 중성지방치 (r=0.3022), 총콜레스테롤과 HDL-콜레스테롤의 비 (r=0.2770), apo B치 (r=0.2927), apo AI과 apo B의 비 (r=-0.3408)와 연관이 있었고 peak stenosis는 중성지방 치(r=0.3305), ape B 치 (r=0.2968), apo AI과 apo B의 비 (r=-0.3977)와 관계가 있었다. 연령으로 조정하여 partial correlation을 좌면, 관상동맥조영술상 중증도는 중성지방치 (r=0.3408), apo B 치(r=0.2877), apo AI과 apo B의 비 (r=-0.3460)와 유의한 상관 관계가 있었다. 이중 ape AI과 ape B의 비는 lesion score (r=-0.2640), total score (r=-0.3057), peak stenosis (r=-0.3460)와 유의한 상관관계를 보였다. 결론 : 이상의 결과로 보아 apo B치 그리고 apo AI과 apo B의 비가 관상동맥 중증도의 예견인자로 다른 혈중 지질 치보다 더 유용하다는 것을 알 수 있었다. Objectives : Recently, it was reported that the measurements of apolipoprotein levels may be valuable in the clinical assessment of coronary artery disease severity. However there are a few reports regarding to the relationship between coronary artery disease severity and apolipoprotein levels in Korea. Thus, we measured serum lipid and apolipoprotein levels and studied the relationship between coronary artery disease severity and serum lipids and apolipoproteins levels. Subjects and Methods : The 69 patients who underwent coronary angiography to evaluate chest pain were subjected to this study. We measured the levels of serum cholesterol, triglycerides, HDL cholesterol, apolipoprotein AI(apo AI) and apolipoprotein B(Apo B) and measured the severity of coronary artery disease by lesion number, lesion score, total score and peak stenosis. Then, we studied the relationship between coronary artery disease severity and apolipoproteins and calculated the correlation coefficient. Results : The results were as follows. There are significant correlation(r=0.3) between age, triglycerides, apo B levels, apo AI/B ratio and coronary artery disease severity. In controlling for age, tyiglycerides, apo B levels and apo AI/B ratio shows significant correlation(r=0.3) with coronary artery disease severity. Especially, apo AI/B ratio shows significant correlation(r=0.3) with lesion number, total score and peak stenosis. Conclusion : These results suggest that the apo B levels and the ratio of apo AI/B can be used as significant independent predictor for coronary artery disease severity rather than other serum lipid levels.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

자각성 이명 환자에서 Ginkgo biloba extracts(Ginexin-F)의 제 3상 임상 효과

임현호,정근,최종욱 釜算慶南耳鼻咽喉科學會 1996 임상이비인후과 Vol.7 No.2

갈근(葛根)으로부터 분리된 puerarin의 항당뇨 효과

임현애,임지선,김정상 경북대학교 농업과학기술연구소 2006 慶北大農學誌 Vol.24 No.-

본 연구에서는 갈근 및 그의 주 이소플라본인 puerarin의 활성을 세포수준에서 분석하였다. 먼저 갈근에 함유된 이소플라본의 양을 분석한 결과, puerarin이 총 이소플라본의 90 % 차지하였다. 다음으로는 puerarin의 항당뇨 활성을 검정한 결과 먼저 탄수화물 및 지방소화효소저해활성에 대해서는 거의 미비한 것으로 나타났으나 인슐린 감수성 및 지방세포의 분화의 유도에 대해서는 농도의존적으로 작용하는 것으로 관찰되었다. 따라서 puerarin은 지방조직내로 포도당의흡수를 촉진함으로서 항당뇨 효능을 발휘하는 것으로 추정된다. In this study we evaluated the anti-diabetic potential of Puerariae Radix and its isoflavone (puerarin) by investigating their inhibitory activities against digestive enzymes, α-amylase, α-glucosidase and lipase and effect on glucose uptake and PPAR γ expression. The activities of carbohydrate digestive enzymes were not inhibited by puerarin. Glucose uptake in differentiated adipocytes was stimulated by puerarin. Furthermore, puerarin enhanced the differentiation of preadipocytes as evaluated by triacylglycerol (TG) accumulation, which is specific for differentiated adipocytes. The effect of puerarin on expression of peroxisome proliferator-activated receptor γ (PPAR-γ) gene, which is associated with obesity and dyslipidemia, was examined by both real-time PCR and reverse transcriptase PCR. The study demonstrated that puerarin increased the expression of PPAR-γ. In conclusion, puerarin showed potential to exert anti-diabetic action by enhancing cellular glucose uptake and thereby TG accumulation in adipocyte tissue.

터키안와 및 상부 종양의 임상적 고찰

임재현,하은주,이종수,이승재,박효일 대한신경외과학회 1993 Journal of Korean neurosurgical society Vol.22 No.2

Tumorous lesion of intra- and suprasellar area showed unique clinical pictures and also was very hazardous and difficult to remove surgically because of the surrounding important anatomical structures. Here, authors analyzed 28 confirmed tumor of this area during last two and half years retrospctively. Histopathologically, 28 cases were classified in 14 pituitary adenoma cased6 cases of non-functioning adenoma, 1 GH-secreting adenoma and 7 cases of prolactinoma), 7 craniopharyngioma cases, 3 meninigioma cases and each one case of germinoma, Rathke's cleft cyst, pituitary abscess and hamartoma. Sexual incidence was 6 : 22 in male vs female ratio. Twenty two cases were measured its diameter over 2cm and they extended to suprasellar area in all cases. Five cases of microadenoma were analyzed and they were all enclosed in sella turcica. Visual impairment was the most frequent and prominent clinical symptom. of which incidence was 78.57% (22 cases). The next frequent symptoms were headache and generalized lethargy. Diabetes insipidus was a presenting symptom in 4 cases and hydrocephalus was noted in 4 cases preoperatively. Surgical intervention was carried out in 23 cases, by the way, the performed type of surgery in 21 cases was a conventional transcranial frontotemporal(pterional) microsurgical method. Post-operatively, diabetes insipidus was complicated in 78.26 % and pituitary hypofunction was noted 34.78%. Two cases of intracranial hemorrhage was developed after surgery and one was died. Through 6 to 28 months follow up. 82.1 % of cases were improved in its clinical status including 7 cured cases.

다발성 외상환자에서 필수적 단순 방사선검사의 의의

임경수,황성오,임종천,이부수,이강현,이진웅 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

In the guidelines of Advanced Trauma Life Support(ATLS), essential plane roentgenograms (chest AP vIew, cervical spine lateral view and pelvis AP view) are recommanded when primary survey and resuscitation phase are finished in care of multiple injured patients. A prospective study was carried on 376 multiple trauma patients who admitted to Wonju Christian Hospital from July, 1995 to October. We analyzed clinical significance of essential X-ray in multiple trauma patients, and classified the patients by their status of mentality. The one is alert group when their Glasgow Coma Scale(GCS) is over 13 point, the other is disoriented mentality group when their GCS are below 12 point. The alert group was redivided by presence of clnical symptoms and/or signs, and clinical significances of essential X-ray were analyzed. Among the 376 patients, 330 patients are alert group that their GCS score is over 13 point and 46 patients are disoriented group who's GCS were below 12 point. The detection rate of abnormality in essential X-ray is higher in disoriented group than alert group(cervical-spine lateral view : 13.5% vs 3.0% cheat AP view : 0.4%, pelvis AP view : 0%). This study suggests that their are no needs of taking essential X-ray in patients who is alert in mental state and have no clinical symptoms and/or signs.

흰쥐 중격측좌핵의 아미노산 함량에 대한 복측 또는 배측 뇌 해마 제거 영향

임종현,김종규 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

A study was planned to analyze amino acid in accumbens septal tissue of dorsal hippocampectomized rats by high-performance liquid chromatography (HPLC). Male albino rats were used. Under chloral hydrate anesthesia (20 %, 2 ㎖/㎏ body weight), a hole was made in each parietal bone about 4 ㎜ lateral to the sagital suture, and 4 ㎜ rostral to the lambda suture. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue anterodorsal to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the dorsal hippocampal animal. The cortical tissue on each side was removed and, through this opening, the hippocampal tissue posteroventral to the hippocampal flexures on both sides was removed by aspiration. This preparation served as the ventral hippocampal animal. The cortical control animal received the same surgery short of hippocampectomy. The normal rats served as normal control animal. One week later, the animals were sacrificed by decapitation in the cold room. two to three milligrams of tissue was obtained from the accumbens septi in one side of the brain. The tissue samples were homogenized in 200 ㎕ of 0.5 M perchloric acid in 1 mM EDTA with ground-glass homogenizers. After centrifugation at 3,500 rpm for 15 min, an aliquot of 80 ㎕ supernatant was neutralized with 20 ㎕ of 2 M KHCO₃, and then centrifuged again at 3,500 rpm for 10 min. An aliquot of 20 ㎕ was taken and diluted with 350 ㎕ of β-mercaptopropionic acid in 10 ml of 100 mM sodium borate buffer (PH 9.1) and 350 ㎕ of OPA solved in ethanol which was diluted with 10 ml of 100 mM sodium borate buffer (PH 9.1). Then 200 ㎕ of the resulting sample was injected with auto injector. Peak areas were automatically integrated and calculated by chromatopac. 1) The contents of aspartate were increased significantly more in the dorsal hippocampal group (dorsal hippocampal group vs normal control group : P < 0.02, dorsal hippocampal group vs cortical control group : P < 0.025), and in the ventral hippocampal group (ventral hippocampal group vs normal control group : P < 0.02, ventral hippocampal group vs cortical control group : P < 0.005) than in the normal control and the cortical control group, while there were no significant differences between the 2 control groups. 2) The contents of glutamate were reduced significantly more in the dorsal hippocampal group (dorsal hippocampal group vs normal control group : P < 0.001, dorsal hippocampal group vs cortical control group : P < 0.001) than in the normal control group and the cortical control group, while there were no significant differences between the 2 control groups. The contents of glutamate were increased significantly more in the ventral hippocampal group (ventral hippocampal group vs normal control group : P < 0.001, ventral hippocampal group vs cortical control group : P < 0.001) than in the normal control group and the cortical control group, while there were no significant differences between the 2 control groups. The contents of glutamate were increased significantly in the ventral hippocampal group than in the dorsal hippocampal group. 3) The contents of alanine were reduced significantly more in the dorsal hippocampal group than in the normal control group (P < 0.001) and the cortical control group (P < 0.001), while there were no significant differences between the 2 control groups. 4) The differences in the contents of serine, glycine and threonine among the 3 group were nonsignificant. It is inferred from the above mentioned results that excitatory transmitter substances employ the glutamate and aspartate in accumbens septi, and that the dorsal hippocampus was facilitatory to the accumbens septi and that the ventral hippocampus was inhibitory to the accumbens septi, and that there was a functional differentiation within the hippocampus.

土地公槪念과 私有財産制와의 法的 葛藤에 관한 硏究

林正平,石琮顯 단국대학교 1986 論文集 Vol.20 No.-