Age-Associated Changes in the Vascular Renin-Angiotensin System in Mice

Yoon, Hye Eun,Kim, Eun Nim,Kim, Min Young,Lim, Ji Hee,Jang, In-Ae,Ban, Tae Hyun,Shin, Seok Joon,Park, Cheol Whee,Chang, Yoon Sik,Choi, Bum Soon Hindawi Publishing Corporation 2016 Oxidative medicine and cellular longevity Vol.2016 No.-

<P><I>Background</I>. This study evaluated whether the change in the renin-angiotensin system (RAS) is associated with arterial aging in mice.<I> Methods</I>. Histologic changes and expressions of transforming growth factor-<I>β</I> (TGF-<I>β</I>), collagen IV,<I> fibronectin</I>, angiotensin II (Ang II), angiotensin-converting enzyme (ACE), angiotensin-converting enzyme 2 (ACE2), angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), prorenin receptor (PRR), Mas receptor (MasR), endothelial nitric oxide synthase (eNOS), NADPH oxidase 2 and oxidase 4 (Nox2 and Nox4), 8-hydroxy-2′-deoxyguanosine (8-OHdG),<I> 3-nitrotyrosine</I>, and superoxide dismutase 1 and dismutase 2 (SOD1 and SOD2) were measured in the thoracic aortas from 2-month-old, 12-month-old, and 24-month-old C57/BL6 mice.<I> Results</I>. Twenty-four-month-old mice showed significantly increased aortic media thickness and expressions of TGF-<I>β</I>, collagen IV, and fibronectin, compared to 2-month-old and 12-month-old mice. The expressions of PRR, ACE, and Ang II, and AT1R-positive area significantly increased, whereas expressions of ACE2 and MasR and AT2R-positive area decreased with age. The expressions of phosphorylated serine<SUP>1177</SUP>-eNOS, SOD1, and SOD2 decreased, and the 8-OHdG-positive area and the 3-nitrotyrosine-positive area increased with age. The expression of Nox2 significantly increased with age, but that of Nox4 did not change.<I> Conclusions</I>. The enhanced PRR-ACE-Ang II-AT1R axis and reduced ACE2-MasR axis were associated with arterial aging in mice. </P>

Treatment of alopecia areata with tofacitinib

( Ji Su Lee ),( Min-woo Kim ),( Hyun-sun Park ),( Jung Yoon Ohn ),( Hyun-sun Yoon ),( Chang-hun Huh ),( Ohsang Kwon ),( Soyun Cho ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.2

Background: Treatment of alopecia areata (AA) is often empiric and not yet satisfactory. Recently, Janus kinase inhibitor showed promising results in Caucasians. Objectives: To investigate efficacy, tolerability, and hair growth trajey of oral tofacitinib monotherapy for Korean AA patients over extended period. Methods: This is a retrospective study of adult AA patients treated with tofacitinib monotherapy for least 4 months. Results: Thirty-six Korean patients with median initial SALT score of 98.9 were included; 91.7% had refractory AA. Twenty-nine (80.6%) patients demonstrated more than 5% SALT change. Twenty patients (55.6%) achieved 50% SALT change at median 7.5 months with 5 mg twice dosage. There were significant differences between SALT 50 achievers and non-achievers: duration of current episode (ρ = 0.009), duration of disease since first onset (ρ = 0.026) and age at onset of first episode (ρ = 0.011). Tofacitinib was well tolerated and there was no any serious adverse effect such as malignancy or tuberculosis reactivation. Conclusion: Oral tofacitinib monotherapy may be effective and tolerable in patients with AA. SALT 50 achievement was associated with followings: duration of current episode, duration of disease since first onset, and age at onset of first episode.

Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

Marked Decreases of Foxp3 and CTLA-4 Are Associated with Strong Antiviral Effects of Tenofovir in Patients with Chronic Hepatitis B

( Ji Young Kim ),( Chang Wook Kim ),( Yun Hui Kim ),( Seok Cheon Yeom ),( Su Gyeong Lee ),( Hee Yeon Kim ),( Si Hyun Bae ),( Jong Young Choi ),( Seung Kew Yoon ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Immune regulatory molecules such as forkhead box P3 (Foxp3) on CD4+ T cell and cytotoxic T lymphocyte-associated antigen 4 CTLA-4) on CD8+ T cell are associated with antiviral effector T cell dysfunction, which influences on T cell exhaustion and persistent viral infection in patients with chronic hepatitis B. These Foxp3 and CTLA-4 are up-regulated in chronic hepatitis B. During antiviral therapy with tenofovir, the expressions of Foxp3 and CTLA-4 could be changed. We investigated the relationship between antiviral effects of tenofovir and the expression of Foxp3 and CTLA-4 during tenofovir treatment in chronic hepatitis B. Methods: Eight patients with chronic hepatitis B under tenofovir treatment were enrolled for detection of Foxp3 on CD4+ T cell and CTLA-4 on CD8+ T cell. Peripheral blood mononuclear cells were isolated from these subjects before tenofovir treatment (T0), 3 month (T3) and 6 month (T6) during tenofovir treatment. For antiviral effect analysis, serum HBV DNA levels were checked at same time. The expressions of Foxp3 and CTLA-4 on T cells were monitored by flow cytometry. Results: Three patients (3 of 8) showed marked decreases of Foxp3 and CTLA-4 during tenofovir therapy (group 1). Five patients (5 of 8) showed minimal changes of Foxp3 or CTLA-4 during tenofovir therapy (group 2). Group 1 showed complete virologic response within 6 month therapy regardless of baseline HBV DNA level but, group 2 showed complete virologic response within 6 month therapy only in patients with low baseline HBV DNA level (< 7log HBV DNA). Conclusions: Among the patients with chronic hepatitis B, the patients who showed marked decrease of Foxp3 and CTLA-4 during tenofovir therapy are associated with strong antiviral effects of tenofovir regardless of baseline HBV DNA level. This finding suggests that restoration of HBV-specific T cell strengthens the antiviral effects of tenofovir.

공동주택 발코니 확장에 따른 결로 방지를 위한 창호 디자인의 실험적 연구

윤대원(Yoon Dae-Won),정창헌(Cheong Chang-Heon),김지영(Kim Ji-Yoeng),유선용(Yoo Seon-Yong),김태연(Kim Tae-Yeon),이승복(Leigh Seung-Bok) 대한건축학회 2008 대한건축학회논문집 Vol.24 No.10

According to the legalization of balcony expansions in apartment houses since January 2006, most of the apartment houses now are built with expanded balcony space. The existing balcony space was physically a buffer between the outside environment and the inside environment. In addition, it was a space that functioned to minimize the indoor environmental changes in relation to external weather changes. However, as apartment houses with extended balconies have become more common recently, the possibility of dew condensation increased due to the weakening of the insulation capacity as the space that can carry out these environmental buffer roles have disappeared. Also, the indoor thermal environment has been weakened. Thus, this paper seeks to resolve problems related to the expansion of balconies by presenting a new method that improves the insulation performance and prevents condensation in the window design that an apartment house with an extended balcony should have. also performance evaluation is executed on new window design to prevent condensation.

위용종의 내시경적 생검과 내시경적 절제술 후 병리학적 진단의 불일치

윤원재 ( Won Jae Yoon ),이동호 ( Dong Ho Lee ),이국래 ( Kook Lae Lee ),장동경 ( Dong Kyung Chang ),김병관 ( Byeong Gwan Kim ),김지원 ( Ji Won Kim ),정지봉 ( Ji Bong Jeong ),김나영 ( Na Young Kim ),김진욱 ( Jin Wook Kim ),황진혁 ( 대한내과학회 2005 대한내과학회지 Vol.69 No.5

Background : Gastric polyp is a descriptive term referring to mucosal prominence that protrudes beyond the flat lining of the stomach. Almost 90% of gastric polyps are hyperplastic polyps. Adenomatous polyps may contain focal carcinomatous foci or undergo carcinomatous changes. It is known that there is some degree of discordance between the results of endoscopic forceps biopsy and pathology of resected specimens. The aim of this study was to investigate the discordance in pathologic findings between endoscopic forceps biopsy and endoscopic resection specimen of gastric polyps. Methods : We reviewed endoscopic photographs and medical records of the patients who underwent endoscopic resection from April, 1996 through February, 2003. Results : A total of 85 cases of gastric polyps from 74 patients were reviewed. Male-to-female ratio was 1:1.96. Mean age was 59.9±10.8 years. Multiple polyps were observed in 10.8%. Gastric polyps occurred in the antrum most frequently (58.8%). Pathology results on resected specimens were as follows: tubular adenoma 45.9%, hyperplastic polyp 31.8%, inflammatory polyp 9.4%, hamartoma 3.5%, fundic gland polyp 2.4%, tubulovillous adenoma 2.4%, adenocarcinoma 2.4%, dysplasia 1.1%, and mucosal pseudolipomatosis 1.1%. Discordance rate between endoscopic biopsy and pathology of resected specimens was 27.1%. There was no relationship between the size of the polyp and concordance rate. Conclusions : There was considerable discordance in pathologic findings between endoscopic forceps biopsy and resected specimens. Approaches to review the histology of an entire polyp should be performed, especially when an adenoma is suspected.(Korean J Med 69:481-486, 2005)

A Multicenter Analysis of Clinical Features and Long-Term Outcomes of POEMS Syndrome in Korea

Kook Hye Won,Jang Ji Eun,Min Chang-Ki,Yoon Dok Hyun,Kim Kihyun,Bang Soo-Mee,Park Yong,Lee Jae Hoon,Yoon Sung-Soo,Kim Jin Seok 대한의학회 2024 Journal of Korean medical science Vol.39 No.2

Background: POEMS syndrome is a rare form of plasma cell dyscrasia characterized by polyneuropathy, organomegaly, endocrinopathy, monoclonal proteins, and skin changes. Owing to its low incidence, there are few reports regarding this syndrome. This multicenter study included 84 patients diagnosed with POEMS syndrome in South Korea. Methods: We retrospectively evaluated 84 patients diagnosed with POEMS syndrome at 8 hospitals in South Korea between January 2000 and October 2022. The clinical characteristics and treatment outcomes were analyzed. Results: The median patient age was 53 years (range, 26–77 years), and 63.1% of the patients were male. All patients had peripheral neuropathy, and 81 (96.4%) had monoclonal plasma cell proliferation. Plasma vascular endothelial growth factor levels were available for 32 patients with a median of 821 pg/mL (range, 26–12,900 pg/mL). Other common features included skin changes (54.2%), volume overload (71.4%), and organomegaly (72.6%). Of the 84 patients, 75 received initial treatment (local radiotherapy, 6 [8.0%]; chemotherapy, 17 [22.7%]; both chemotherapy and local radiotherapy, 9 [12.0%]), upfront autologous stem cell transplantation (ASCT), 43 (57.3%; with induction chemotherapy, n = 12, 16.0%; without induction chemotherapy, n = 31, 41.3%). The median follow-up duration was 40.7 months. The 5-year overall survival (OS) was 78%, and the 5-year progression-free survival (PFS) was 55%. Patients who underwent upfront ASCT and were diagnosed after 2014 had a longer OS and PFS. Conclusion: The demographics of Korean patients with POEMS syndrome were similar to those reported previously. Because of the introduction of new treatment agents and the reduced rate of transplant-related mortality related to ASCT, the treatment outcomes of Korean patients with POEMS syndrome have improved in recent years.

Gene Expression Analysis of the Bromobenzene Treated Liver with Non-hepatotoxic Doses in Mice

Lim, Jung-Sun,Jeong, Sun-Young,Hwang, Ji-Yoon,Park, Han-Jin,Cho, Jae-Woo,Song, Chang-Woo,Kim, Yang-Seok,Lee, Wan-Seon,Moon, Jin-Hee,Han, Sang-Seop,Yoon, Seok-Joo The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.4

Bromobenzene (BB) is well known hepatotoxicant. Also, BB is an industrial solvent that arouses toxicity predominantly in the liver where it causes centrilobular necrosis. BB is subjected to Cytochrome P450 mediated epoxidation followed by either conjugation with glutathione, enzymatic hydrolysis or further oxidation. In this study, we focused on BB-induced gene expression at non-hepatotoxic dose. Mice were exposed to two levels of BB, sampled at 24 h, and hepatic gene expression levels were determined to evaluate dose dependent changes. When examining the toxic dose of BB treated group in other previous studies, genes related to heat shock protein, oxidative stress, and drug metabolism are expressed. Compared to these results, our study, in which non-toxic dose of BB was administrated, showed similar patterns as the toxic conditions above. The purpose of the study was to select genes that showed changes in relation to the differing dose through confirmation of the difference within transcriptomic boundaries, but those that are not detected by the existing classic toxicology tools in non-hepatotoxic dose.

나프타분해플랜트의 부탄추출공정에서 부탄증기의 연속누출에 의한 화재사고의 영향평가

윤대건,이헌창,함병호,조지훈,김태옥 韓國火災 ·消防學會 1998 한국화재소방학회논문지 Vol.10 No.4

나프타분해플랜트의 부탄추출공정에서 부탄증기의 연속 누출에 의한 제트화재와 플래쉬화재의 사고결과에 대한 영향을 평가하고 사고결과에 미치는 공정변수들의 영향을 해석하였다. 877 kPa과 346.75K의 공정조건에서 부탄증기가 87.8 kg/s의 속도로 8m의 높이에서 연속누출되는 경우에 표준조건으로 설정한 대기 및 환경조건에서 사고결과를 해석한 결과, 제트화재에서는 화염의 형태와 크기를 예측할 수 있었으며 누출지점으로부터 200m에서 API모델로 산출한 목사열은 약 5kW/m^2이었고 플래쉬화재가 일어날 수 있는 연소범위는 11.2~120.2m이었다. 그리고 사고결과에 미치는 조업압력의 영향은 조업온도보다 크며 압력이 증가할수록 사고결과 값이 증가하였다. 이때 플래쉬화재에서 X_UFL에 미치는 공정압력의 영향은 X_LFL에서보다 약 1/10이하를 나타내었다. The consequence analysis for jet and flash fire accidents by the continuous release of butane vapor was performed and effects of process variables on consequences were analyzed in standard conditions. For the continuous release(87.8 kg/s) of butane vapor at 8 m elevated height in the debutanizing process of the naphtha cracking plant operating at 877 kPa, 346.75 K, we found that for the jet fire accident, shape and size of the flame could be predicted and thermal radiation estimated by API model at 200 m distance from release point was 1.5 kW/m^2, and that for the flash fire accident, effect range was 11.2~120.2m. Also, simulation results showed that effects of operating pressures on consequences were large than those of operating temperatures and results of accidents were increased with increasing operating pressures. At this time, effects of operating pressures on X_UFL. were smaller

골수염으로 발현된 재발성 콕시디오이데스 진균증 1예

백지현,박은영,정윤숙,홍재원,채윤태,진성준,최희경,신소연,한상훈,진범식,김창오,최준용,송영구,조남훈,김준명 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Coccidioidomycosis is a fungal infection that results from inhaling the airborne arthroconidia of the Coccidioides species. It is an endemic disease in the southwest part of North America and rarely diagnosed in Korea. As tourism to endemic areas and the number of immunocompromised patients have been increasing, the incidence of this infection has increased in non-endemic areas. Treatment is usually successful with antifungal agents; however, recurrence is common. It is difficult to decide when to discontinue the antifungal treatment especially in non-endemic areas where doctors are not familiar with the disease. We report a case of recurrent coccidioidomycosis manifesting as osteomyelitis after the treatment of the patient for disseminated coccidioidal infection. The complement fixation test was a useful tool for the assessment of patient response and to evaluate suspected recurrence.